Abstract:
:Apoptotic cell volume decrease (AVD) and exposure of phosphatidylserine (PtdSer) at the cell surface are early events in apoptosis. However, the ion channels responsible for AVD, and their relationship to PtdSer translocation and cell death are poorly understood. Real-time analysis of calcium-induced apoptosis in lymphocytes and thymocytes showed that AVD occurs rapidly, and precedes PtdSer translocation. Blockers of the K(+) channel IKCa1 completely inhibited AVD. Blockade of IKCa1, and hence AVD, also completely prevented PtdSer translocation and cell death. Thus, IKCa1-mediated AVD is the earliest-defined essential step in calcium-induced apoptosis, required for both PtdSer translocation and cell death.
journal_name
EMBO Repjournal_title
EMBO reportsauthors
Elliott JI,Higgins CFdoi
10.1038/sj.embor.embor722keywords:
subject
Has Abstractpub_date
2003-02-01 00:00:00pages
189-94issue
2eissn
1469-221Xissn
1469-3178pii
embor722journal_volume
4pub_type
杂志文章相关文献
EMBO REPORTS文献大全abstract::Microbes exert a major impact on human health and disease by either promoting or disrupting homeostasis, in the latter instance leading to the development of infectious diseases. Such disparate outcomes are driven by the ever-evolving genetic diversity of microbes and the countervailing host responses that minimize th...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.15252/embr.201540558
更新日期:2015-11-01 00:00:00
abstract::WDR11 has been implicated in congenital hypogonadotropic hypogonadism (CHH) and Kallmann syndrome (KS), human developmental genetic disorders defined by delayed puberty and infertility. However, WDR11's role in development is poorly understood. Here, we report that WDR11 modulates the Hedgehog (Hh) signalling pathway ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201744632
更新日期:2018-02-01 00:00:00
abstract::Differentiation of most cell types requires both establishment of G1 arrest and the induction of a program related to achieving quiescence. We have chosen to study the differentiation of oligodendrocyte cells to determine the role of p27 and p21 in this process. Here we report that both p27 and p21 are required for th...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1093/embo-reports/kve008
更新日期:2001-01-01 00:00:00
abstract::Intercellular communication orchestrates effective immune responses against disease-causing agents. Extracellular vesicles (EVs) are potent mediators of cell-cell communication. EVs carry bioactive molecules, including microRNAs, which modulate gene expression and function in the recipient cell. Here, we show that for...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201948925
更新日期:2020-04-03 00:00:00
abstract::At gastrulation, a subpopulation of epiblast cells constitutes a transient posteriorly located structure called the primitive streak, where cells that undergo epithelial-mesenchymal transition make up the mesoderm and endoderm lineages. Mouse embryo epiblast cells were labelled ubiquitously or in a mosaic fashion. Cel...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.202050944
更新日期:2020-11-05 00:00:00
abstract::Tie2, an endothelial cell-specific receptor kinase, has an important role in tumour angiogenesis. In an attempt to identify peptides that specifically interact with and block the Tie2 pathway, a phage-displayed peptide library was screened on a recombinant Tie2 receptor. One peptide, NLLMAAS, completely abolished the ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400100
更新日期:2004-03-01 00:00:00
abstract::Cleavage of the cohesin subunit Scc1p/Mcd1p/Rad21 permits sister chromatid separation and is considered to trigger anaphase onset. It has also been suggested that the cohesin complex is essential for chromosome condensation and for assembling fully functional kinetochores. Here, we used vertebrate cells conditionally ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400077
更新日期:2004-02-01 00:00:00
abstract::Histone demethylases-both lysine-specific demethylase 1 (LSD1) and Jumonji-C (JmjC) domain-containing proteins-are broadly implicated in the regulation of chromatin-dependent processes. In Arabidopsis thaliana, histone marks directly affect DNA methylation, and mutations in LSD1 homologues show reduced DNA methylation...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2010.158
更新日期:2010-12-01 00:00:00
abstract::Mammalian artificial chromosomes (MACs) provide a new tool for the improvement of our knowledge of chromosome structure and function. Moreover, they constitute an alternative and potentially powerful tool for gene delivery both in cultured cells and for the production of transgenic animals. In the present work we desc...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1093/embo-reports/kve018
更新日期:2001-02-01 00:00:00
abstract::The high-resolution spatial induction of ultraviolet (UV) photoproducts in mammalian cellular DNA is a goal of many scientists who study UV damage and repair. Here we describe how UV photoproducts can be induced in cellular DNA within nanometre dimensions by near-diffraction-limited 750 nm infrared laser radiation. Th...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400028
更新日期:2003-12-01 00:00:00
abstract::One limitation for the study of chromosomal fragile sites is that they must be studied on metaphase spreads, after the breakage. We show here that bacterial lac operator (lacO) repeats are prone to spontaneous breakage, which when combined with a fluorescent lac repressor (lacR) has allowed us to track a fragile site ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2011.158
更新日期:2011-09-30 00:00:00
abstract::The intracellular modification of proteins by the addition of a single O-linked N-acetylglucosamine (O-GlcNAc) molecule is a ubiquitous post-translational modification in eukaryotic cells. It is catalysed by O-linked N-acetylglucosaminyltransferase, which attaches O-GlcNAc to serine/threonine residues, and it is count...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.1038/embor.2008.129
更新日期:2008-08-01 00:00:00
abstract::Myc is an oncoprotein transcription factor that promotes cell proliferation and apoptosis. Analysis of highly conserved elements within vertebrate Myc proteins has been instrumental in defining the functions of the Myc protein. Here, we probe the role of a highly conserved, but little studied, element within the centr...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400333
更新日期:2005-02-01 00:00:00
abstract::The Ark/Prk serine/threonine kinases initiate phosphorylation cycles that control the endocytic machinery in mammalian cells and in yeast, and the actin cytoskeleton in yeast. The members of this protein family are unified by homologies in their kinase domain, but are generally diverse in their other domains. The evol...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.1038/sj.embor.embor776
更新日期:2003-03-01 00:00:00
abstract::The crucial role of individual Notch receptors and the mechanism by which they maintain intestinal crypt progenitor cells were assessed by using a series of inducible gut-specific Notch mutant mice. We found that Notch1 and Notch2 receptors function redundantly in the gut, as only simultaneous loss of both receptors r...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2008.7
更新日期:2008-04-01 00:00:00
abstract::Activation of the elongation factor 2 kinase (eEF2K) leads to the phosphorylation and inhibition of the elongation factor eEF2, reducing mRNA translation rates. Emerging evidence indicates that the regulation of factors involved in protein synthesis may be critical for controlling diverse biological processes includin...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201642194
更新日期:2016-10-01 00:00:00
abstract::Scrapie, bovine spongiform encephalopathy and chronic wasting disease are orally communicable, transmissible spongiform encephalopathies (TSEs). As zoonotic transmissions of TSE agents may pose a risk to human health, the identification of reservoirs for infectivity in animal tissues and their exclusion from human con...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.embor827
更新日期:2003-05-01 00:00:00
abstract::In multicellular epithelial tissues, the orientation of polarity of each cell must be coordinated. Previously, we reported that for Madin-Darby canine kidney cells in three-dimensional collagen gel culture, blockade of beta1-integrin by the AIIB2 antibody or expression of dominant-negative Rac1N17 led to an inversion ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2008.135
更新日期:2008-09-01 00:00:00
abstract::Despite its limited resources, Portugal has gained a prominent position in research on malaria. Several historical and personal factors have contributed to this achievement. ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.202051692
更新日期:2021-01-10 00:00:00
abstract::When our time comes to die most people would probably opt for a quick, peaceful and painless exit. But the manner and timing are rarely under our direct control. Hence the Ars moriendi, literally, "The Art of Dying", two texts written in Latin around the 15th century that offered advice on how to die well according to...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.1002/embr.201337970
更新日期:2014-02-01 00:00:00
abstract::Anopheles gambiae, the major vector of human malaria parasite, is an important insect model to study vector-parasite interactions. Here, we developed a simple in vivo double-stranded RNA (dsRNA) knockout approach to determine the function of the mosquito antimicrobial peptide gene Defensin. We injected dsRNA into adul...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1093/embo-reports/kvf180
更新日期:2002-09-01 00:00:00
abstract::The tumor suppressor Smad4, a key mediator of the TGF-β/BMP pathways, is essential for development and tissue homeostasis. Phosphorylation of Smad4 in its linker region catalyzed by the mitogen-activated protein kinase (MAPK) plays a pivotal role in regulating its transcriptional activity and stability. In contrast, r...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201948693
更新日期:2020-05-06 00:00:00
abstract::The ubiquitin ligase Highwire restrains synaptic growth and promotes evoked neurotransmission at NMJ synapses in Drosophila Highwire regulates synaptic morphology by downregulating the MAP3K Wallenda, but excess Wallenda signaling does not account for the decreased presynaptic release observed in highwire mutants. Hen...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201846975
更新日期:2019-03-01 00:00:00
abstract::In Drosophila, the piRNA cluster, flamenco, produces most of the piRNAs (PIWI-interacting RNAs) that silence transposable elements in the somatic follicle cells during oogenesis. These piRNAs are thought to be processed from a long single-stranded precursor transcript. Here, we demonstrate that flamenco transcription ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1002/embr.201337898
更新日期:2014-04-01 00:00:00
abstract::Epidermal growth factor (EGF) activates the EGF receptor (EGFR) and stimulates its internalization and trafficking to lysosomes for degradation. However, a percentage of EGFR undergoes ligand-independent endocytosis and is rapidly recycled back to the plasma membrane. Importantly, alterations in EGFR recycling are a c...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201744034
更新日期:2017-12-01 00:00:00
abstract::Subcellular organization is yielding to large-scale analysis. Researchers are now applying robust mass-spectrometry-based proteomics methods to obtain an inventory of biochemically isolated organelles that contain hundreds of proteins. High-resolution methods allow accurate protein identification, and novel algorithms...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.1038/sj.embor.7400780
更新日期:2006-09-01 00:00:00
abstract::Parkin, a product of the causative gene of autosomal-recessive juvenile parkinsonism (AR-JP), is a RING-type E3 ubiquitin ligase and has an amino-terminal ubiquitin-like (Ubl) domain. Although a single mutation that causes an Arg to Pro substitution at position 42 of the Ubl domain (the Arg 42 mutation) has been ident...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.embor764
更新日期:2003-03-01 00:00:00
abstract::The receptor-evoked Ca(2+) signal is sensed and translated by mitochondria. Physiological cytoplasmic Ca(2+) ([Ca(2+)]c) oscillations result in mitochondrial Ca(2+) ([Ca(2+)]m) oscillations, while large and sustained [Ca(2+)]c increase results in a pathologic increase in basal [Ca(2+)]m and in Ca(2+) accumulation. The...
journal_title:EMBO reports
pub_type: 评论,杂志文章
doi:10.1002/embr.201438446
更新日期:2014-03-01 00:00:00
abstract::Although many signal transduction pathways have been implicated in the development of human disease, the identification of pathway targets and the biological processes that mediate disease progression remains challenging. One such disease-related pathway is the Janus kinase (JAK)/signal transducer and activator of tra...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2010.1
更新日期:2010-03-01 00:00:00
abstract::Hypoxic and oxidant stresses can coexist in biological systems, and oxidant stress has been proposed to activate hypoxia pathways through the inactivation of the 'oxygen-sensing' hypoxia-inducible factor (HIF) prolyl and asparaginyl hydroxylases. Here, we show that despite reduced sensitivity to cellular hypoxia, the ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2012.9
更新日期:2012-03-01 00:00:00