Abstract:
:The high-resolution spatial induction of ultraviolet (UV) photoproducts in mammalian cellular DNA is a goal of many scientists who study UV damage and repair. Here we describe how UV photoproducts can be induced in cellular DNA within nanometre dimensions by near-diffraction-limited 750 nm infrared laser radiation. The use of multiphoton excitation to induce highly localized DNA damage in an individual cell nucleus or mitochondrion will provide much greater resolution for studies of DNA repair dynamics and intracellular localization as well as intracellular signalling processes and cell-cell communication. The technique offers an advantage over the masking method for localized irradiation of cells, as the laser radiation can specifically target a single cell and subnuclear structures such as nucleoli, nuclear membranes or any structure that can be labelled and visualized by a fluorescent tag. It also increases the time resolution with which migration of DNA repair proteins to damage sites can be monitored. We define the characteristics of localized DNA damage induction by near-infrared radiation and suggest how it may be used for new biological investigations.
journal_name
EMBO Repjournal_title
EMBO reportsauthors
Meldrum RA,Botchway SW,Wharton CW,Hirst GJdoi
10.1038/sj.embor.7400028keywords:
subject
Has Abstractpub_date
2003-12-01 00:00:00pages
1144-9issue
12eissn
1469-221Xissn
1469-3178pii
7400028journal_volume
4pub_type
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