Abstract:
:Mammalian artificial chromosomes (MACs) provide a new tool for the improvement of our knowledge of chromosome structure and function. Moreover, they constitute an alternative and potentially powerful tool for gene delivery both in cultured cells and for the production of transgenic animals. In the present work we describe the molecular structure of MC1, a human minichromosome derived from chromosome 1. By means of restriction and hybridization analysis, satellite-PCR, in situ hybridization on highly extended chromatin fibres, and indirect immunofluorescence, we have established that: (i) MC1 has a size of 5.5 Mb; (ii) it consists of 1.1 Mb alphoid, 3.5 Mb Sat2 DNA, and telomeric and subtelomeric sequences at both ends; (iii) it contains an unusual region of interspersed Sat2 and alphoid DNAs at the junction of the alphoid and the Sat2 blocks; and (iv) the two alphoid blocks and the Sat2-alphoid region bind centromeric proteins suggesting that they participate in the formation of a functional kinetochore.
journal_name
EMBO Repjournal_title
EMBO reportsauthors
Auriche C,Donini P,Ascenzioni Fdoi
10.1093/embo-reports/kve018keywords:
subject
Has Abstractpub_date
2001-02-01 00:00:00pages
102-7issue
2eissn
1469-221Xissn
1469-3178journal_volume
2pub_type
杂志文章相关文献
EMBO REPORTS文献大全abstract::RNA silencing is a complex of mechanisms that regulate gene expression through small RNA molecules. The microRNA (miRNA) pathway is the most common of these in mammals. Genome-encoded miRNAs suppress translation in a sequence-specific manner and facilitate shifts in gene expression during developmental transitions. He...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.1038/embor.2010.102
更新日期:2010-08-01 00:00:00
abstract::Plexins are receptors for the axonal guidance molecules known as semaphorins, and the semaphorin 4D (Sema4D) receptor plexin-B1 induces repulsive responses by functioning as an R-Ras GTPase-activating protein (GAP). Here we characterized the downstream signalling of plexin-B1-mediated R-Ras GAP activity, inducing grow...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400737
更新日期:2006-07-01 00:00:00
abstract::Nuclear components are functionally linked with the dynamic temporal and spatial compartmentalization, sorting and integration of regulatory information to facilitate its selective use. For example, the subnuclear targeting of transcription factors to punctate sites in the interphase nucleus mechanistically couples ch...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.1038/sj.embor.7400337
更新日期:2005-02-01 00:00:00
abstract::Histone demethylases-both lysine-specific demethylase 1 (LSD1) and Jumonji-C (JmjC) domain-containing proteins-are broadly implicated in the regulation of chromatin-dependent processes. In Arabidopsis thaliana, histone marks directly affect DNA methylation, and mutations in LSD1 homologues show reduced DNA methylation...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2010.158
更新日期:2010-12-01 00:00:00
abstract::The discovery that adult somatic cells can be reprogrammed into pluripotent cells by expressing a combination of factors associated with pluripotency holds immense promise for a wide range of biotechnological and therapeutic applications. However, some hurdles-such as improving the low reprogramming efficiencies and e...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2010.47
更新日期:2010-05-01 00:00:00
abstract::Epidermal growth factor (EGF) activates the EGF receptor (EGFR) and stimulates its internalization and trafficking to lysosomes for degradation. However, a percentage of EGFR undergoes ligand-independent endocytosis and is rapidly recycled back to the plasma membrane. Importantly, alterations in EGFR recycling are a c...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201744034
更新日期:2017-12-01 00:00:00
abstract::Intercellular communication orchestrates effective immune responses against disease-causing agents. Extracellular vesicles (EVs) are potent mediators of cell-cell communication. EVs carry bioactive molecules, including microRNAs, which modulate gene expression and function in the recipient cell. Here, we show that for...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201948925
更新日期:2020-04-03 00:00:00
abstract::Estrogen receptors (ERs) orchestrate both transcriptional and non-genomic functions in response to estrogens, xenoestrogens and signals emanating from growth factor signalling pathways. The pleiotropic and tissue-specific effects of estrogens are likely to be mediated by the differential expression of distinct estroge...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.1093/embo-reports/kve185
更新日期:2001-09-01 00:00:00
abstract::The ubiquitin ligase Highwire restrains synaptic growth and promotes evoked neurotransmission at NMJ synapses in Drosophila Highwire regulates synaptic morphology by downregulating the MAP3K Wallenda, but excess Wallenda signaling does not account for the decreased presynaptic release observed in highwire mutants. Hen...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201846975
更新日期:2019-03-01 00:00:00
abstract::We employed electron cryo-tomography to visualize cytosolic ribosomes on the surface of mitochondria. Translation-arrested ribosomes reveal the clustered organization of the TOM complex, corroborating earlier reports of localized translation. Ribosomes are shown to interact specifically with the TOM complex, and nasce...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201744261
更新日期:2017-10-01 00:00:00
abstract::The crystal structure of the ligand-binding domain of RARbeta, a suspect tumour suppressor, reveals important features that distinguish it from the two other RAR isotypes. The most striking difference is an extra cavity allowing RARbeta to bind more bulky agonists. Accordingly, we identified a ligand that shows RARbet...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400235
更新日期:2004-09-01 00:00:00
abstract::The high-mobility-group B (HMGB) chromosomal proteins are characterized by the HMG box, a DNA-binding domain that both introduces a tight bend into DNA and binds preferentially to a variety of distorted DNA structures. The HMGB proteins seem to act primarily as architectural facilitators in the manipulation of nucleop...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.1038/sj.embor.embor741
更新日期:2003-02-01 00:00:00
abstract::Reading the academic literature is an essential part of a scientist's job. David Smith argues that reading fiction can be even more important for creativity and productivity than papers and reviews. ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.202052206
更新日期:2021-01-04 00:00:00
abstract::Hydrogen sulfide (H2 S), an endogenous signaling gaseous molecule, is involved in various physiological activities, including vessel relaxation, regulation of cellular bioenergetics, inflammation, and angiogenesis. By using xenograft orthotopic implantation of prostate cancer PC3 cells and subsequently comparing bone ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201845986
更新日期:2019-10-04 00:00:00
abstract::Defects in protein folding and the proteasomal pathway have been linked with many neurodegenerative diseases. PLIC-1 (protein linking IAP to the cytoskeleton) is a ubiquitin-like protein that binds to the ubiquitin-interacting motif (UIM) of the proteasomal subunit S5a. Here, we show that PLIC-1 also binds to the UIM ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400823
更新日期:2006-12-01 00:00:00
abstract::Vascular endothelial growth factor (VEGF) is the master regulator of angiogenesis, whose best-understood mechanism is sprouting. However, therapeutic VEGF delivery to ischemic muscle induces angiogenesis by the alternative process of intussusception, or vascular splitting, whose molecular regulation is essentially unk...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201745054
更新日期:2018-05-01 00:00:00
abstract::Ubiquitin is a post-translational modifier with proteolytic and non-proteolytic roles in many biological processes. At mitochondria, it performs regulatory homeostatic functions and contributes to mitochondrial quality control. Ubiquitin is essential for mitochondrial fusion, regulates mitochondria-ER contacts, and pa...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.1002/embr.201338225
更新日期:2014-03-01 00:00:00
abstract::Protein kinase D (PKD) mediates the actions of stimuli that promote diacylglycerol (DAG) biogenesis. By phosphorylating effectors that regulate transcription, fission and polarized transport of Golgi vesicles, as well as cell migration and survival after oxidative stress, PKDs substantially expand the range of physiol...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.1038/embor.2011.139
更新日期:2011-07-08 00:00:00
abstract::Alternative RNA processing of LMNA pre-mRNA produces three main protein isoforms, that is, lamin A, progerin, and lamin C. De novo mutations that favor the expression of progerin over lamin A lead to Hutchinson-Gilford progeria syndrome (HGPS), providing support for the involvement of LMNA processing in pathological a...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1002/embr.201338126
更新日期:2014-05-01 00:00:00
abstract::In Drosophila, the piRNA cluster, flamenco, produces most of the piRNAs (PIWI-interacting RNAs) that silence transposable elements in the somatic follicle cells during oogenesis. These piRNAs are thought to be processed from a long single-stranded precursor transcript. Here, we demonstrate that flamenco transcription ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1002/embr.201337898
更新日期:2014-04-01 00:00:00
abstract::Sister chromatid cohesion is the basis for the recognition of chromosomal DNA replication products for their bipolar segregation in mitosis. Fundamental to sister chromatid cohesion is the ring-shaped cohesin complex, which is loaded onto chromosomes long before the initiation of DNA replication and is thought to hold...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2009.207
更新日期:2009-10-01 00:00:00
abstract::Sulphatases undergo a unique post-translational modification that converts a highly conserved cysteine located within their active site into formylglycine. This modification is necessary for the catalytic activities of the sulphatases, and it is generated by the protein product of sulphatase-modifying factor 1 (SUMF1)...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400454
更新日期:2005-07-01 00:00:00
abstract::SUMOylation is a post-translational modification that positively regulates monoallelic expression of the trypanosome variant surface glycoprotein (VSG). The presence of a highly SUMOylated focus associated with the nuclear body, where the VSG gene is transcribed, further suggests an important role of SUMOylation in re...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201948029
更新日期:2019-12-05 00:00:00
abstract::The developmental and oncogenic roles of MYC proteins are well established, but the transcriptional targets mediating their functions remain elusive. Using small interfering RNA-mediated knockdown in breast and cervix carcinoma cell lines, which overexpress c-MYC, we show that c-MYC independently controls metabolism a...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400849
更新日期:2007-01-01 00:00:00
abstract::Given that ligand binding is essential for the rapid internalization of epidermal growth factor receptor (EGFR), the events induced by ligand binding probably contribute to the regulation of EGFR internalization. These events include receptor dimerization, activation of intrinsic tyrosine kinase activity and autophosp...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400491
更新日期:2005-10-01 00:00:00
abstract::The receptor-evoked Ca(2+) signal is sensed and translated by mitochondria. Physiological cytoplasmic Ca(2+) ([Ca(2+)]c) oscillations result in mitochondrial Ca(2+) ([Ca(2+)]m) oscillations, while large and sustained [Ca(2+)]c increase results in a pathologic increase in basal [Ca(2+)]m and in Ca(2+) accumulation. The...
journal_title:EMBO reports
pub_type: 评论,杂志文章
doi:10.1002/embr.201438446
更新日期:2014-03-01 00:00:00
abstract::The accuracy of large-scale DNA sequencing is difficult to estimate without redundant effort. We have found that the mobile genetic element IS10, a component of the transposon Tn10, has contaminated a significant number of clones in the public databases, as a result of the use of the transposon in bacterial cloning st...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1093/embo-reports/kvd015
更新日期:2000-07-01 00:00:00
abstract::Accumulation of unfolded proteins in the endoplasmic reticulum triggers the unfolded protein response (UPR). How the UPR is downregulated is not well understood. Inositol requirement 1 (Ire1) is an endoplasmic reticulum transmembrane UPR sensor in Saccharomyces cerevisiae. When the UPR is triggered, Ire1 is autophosph...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400813
更新日期:2006-11-01 00:00:00
abstract::Ligand-dependent transcriptional activation of retinoic acid receptors (RARs) is a multistep process culminating in the formation of a multimeric co-activator complex on regulated promoters. Several co-activator complexes harbor an acetyl transferase activity, which is required for retinoid-induced transcription of re...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1093/embo-reports/kvf066
更新日期:2002-04-01 00:00:00
abstract::Cardiac dysfunctions dramatically increase with age. Revealing a currently unknown contributor to cardiac ageing, we report the age-dependent, cardiac-specific accumulation of the lysosphingolipid sphinganine (dihydrosphingosine, DHS) as an evolutionarily conserved hallmark of the aged vertebrate heart. Mechanisticall...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201847407
更新日期:2019-04-01 00:00:00