Abstract:
:In Drosophila, the piRNA cluster, flamenco, produces most of the piRNAs (PIWI-interacting RNAs) that silence transposable elements in the somatic follicle cells during oogenesis. These piRNAs are thought to be processed from a long single-stranded precursor transcript. Here, we demonstrate that flamenco transcription is initiated from an RNA polymerase II promoter containing an initiator motif (Inr) and downstream promoter element (DPE) and requires the transcription factor, Cubitus interruptus. We show that the flamenco precursor transcript undergoes differential alternative splicing to generate diverse RNA precursors that are processed to piRNAs. Our data reveal dynamic processing steps giving rise to piRNA cluster precursors.
journal_name
EMBO Repjournal_title
EMBO reportsauthors
Goriaux C,Desset S,Renaud Y,Vaury C,Brasset Edoi
10.1002/embr.201337898subject
Has Abstractpub_date
2014-04-01 00:00:00pages
411-8issue
4eissn
1469-221Xissn
1469-3178pii
embr.201337898journal_volume
15pub_type
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