Abstract:
:Post-translational protein arginylation is essential for cardiovascular development and angiogenesis in mice and is mediated by arginyl-transfer RNA-protein transferases Ate1-a functionally conserved but poorly understood class of enzymes. Here, we used sequence analysis to detect the evolutionary relationship between the Ate1 family and bacterial FemABX family of aminoacyl-tRNA-peptide transferases, and to predict the functionally important residues in arginyltransferases, which were then used to construct a panel of mutants for further molecular dissection of mouse Ate1. Point mutations of the residues in the predicted regions of functional importance resulted in changes in enzymatic activity, including complete inactivation of mouse Ate1; other mutations altered its substrate specificity. Our results provide the first insights into the mechanisms of Ate1-mediated arginyl transfer reaction and substrate recognition, and define a new protein superfamily called Dupli-GNAT to reflect its origin by the duplication of the GNAT acetyltransferase domain.
journal_name
EMBO Repjournal_title
EMBO reportsauthors
Rai R,Mushegian A,Makarova K,Kashina Adoi
10.1038/sj.embor.7400747subject
Has Abstractpub_date
2006-08-01 00:00:00pages
800-5issue
8eissn
1469-221Xissn
1469-3178pii
7400747journal_volume
7pub_type
杂志文章相关文献
EMBO REPORTS文献大全abstract::The influence of circadian rhythms on memory has long been studied; however, the molecular prerequisites for their interaction remain elusive. The hippocampus, which is a region of the brain important for long-term memory formation and temporary maintenance, shows circadian rhythmicity in pathways central to the memor...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.1038/embor.2009.123
更新日期:2009-06-01 00:00:00
abstract::Nicotinamide adenine dinucleotide (NAD) and its phosphorylated form (NADP) are vital for cell function in all organisms and form cofactors to a host of enzymes in catabolic and anabolic processes. NAD(P) transhydrogenases (NTHs) catalyse hydride ion transfer between NAD(H) and NADP(H). Membrane-bound NTH isoforms resi...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201947832
更新日期:2020-03-04 00:00:00
abstract::X inactivation is the mechanism by which mammals adjust the genetic imbalance that arises from the different numbers of gene-rich X-chromosomes between the sexes. The dosage difference between XX females and XY males is functionally equalized by silencing one of the two X chromosomes in females. This dosage-compensati...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.1038/sj.embor.7400871
更新日期:2007-01-01 00:00:00
abstract::Hypoxic and oxidant stresses can coexist in biological systems, and oxidant stress has been proposed to activate hypoxia pathways through the inactivation of the 'oxygen-sensing' hypoxia-inducible factor (HIF) prolyl and asparaginyl hydroxylases. Here, we show that despite reduced sensitivity to cellular hypoxia, the ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2012.9
更新日期:2012-03-01 00:00:00
abstract::Blood cells play a crucial role in both morphogenetic and immunological processes in Drosophila, yet the factors regulating their proliferation remain largely unknown. In order to address this question, we raised antibodies against a tumorous blood cell line and identified an antigenic determinant that marks the surfa...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1093/embo-reports/kvf242
更新日期:2002-12-01 00:00:00
abstract::Like many transcription regulators, histone methyltransferases G9a and G9a-like protein (GLP) can act gene-specifically as coregulators, but mechanisms controlling this specificity are mostly unknown. We show that adjacent post-translational methylation and phosphorylation regulate binding of G9a and GLP to heterochro...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201744060
更新日期:2017-08-01 00:00:00
abstract::Pair-rule genes in Drosophila integrate the positional information provided by the transcription factor gradients of gap genes and set the framework for the expression of the segment polarity genes. Pair-rule genes are also expressed during the segmentation of the short germ-band embryo of the flour beetle Tribolium, ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400148
更新日期:2004-06-01 00:00:00
abstract::The angiotensin II type 1 (AT(1)) receptor is a G protein-coupled receptor that has a crucial role in the development of load-induced cardiac hypertrophy. Here, we show that cell stretch leads to activation of the AT(1) receptor, which undergoes an anticlockwise rotation and a shift of transmembrane (TM) 7 into the li...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7401157
更新日期:2008-02-01 00:00:00
abstract::The human XAB1/MBDin GTPase and its close homologues form one of the ten phylogenetically distinct families of the SIMIBI (after signal recognition particle, MinD and BioD) class of phosphate-binding loop NTPases. The genomic context and the partners identified for the archaeal and eukaryotic homologues indicate that ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400958
更新日期:2007-06-01 00:00:00
abstract::The developmental and oncogenic roles of MYC proteins are well established, but the transcriptional targets mediating their functions remain elusive. Using small interfering RNA-mediated knockdown in breast and cervix carcinoma cell lines, which overexpress c-MYC, we show that c-MYC independently controls metabolism a...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400849
更新日期:2007-01-01 00:00:00
abstract::The cytosolic pathogen sensor RIG-I is activated by RNAs with exposed 5'-triphosphate (5'-ppp) and terminal double-stranded structures, such as those that are generated during viral infection. RIG-I has been shown to translocate on dsRNA in an ATP-dependent manner. However, the precise role of the ATPase activity in R...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2013.102
更新日期:2013-09-01 00:00:00
abstract::HOIL-1L and its binding partner HOIP are essential components of the E3-ligase complex that generates linear ubiquitin (Ub) chains, which are critical regulators of NF-κB activation. Using crystallographic and mutational approaches, we characterize the unexpected structural basis for the specific interaction between t...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2012.24
更新日期:2012-05-01 00:00:00
abstract::Initiation of DNA replication from the Escherichia coli chromosomal origin is highly regulated, assuring that replication occurs precisely once per cell cycle. Three mechanisms for regulation of replication initiation have been proposed: titration of free DnaA initiator protein by the datA locus, sequestration of newl...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400467
更新日期:2005-08-01 00:00:00
abstract::The impact of the mitochondrial permeability transition (MPT) on cellular physiology is well characterized. In contrast, the composition and mode of action of the permeability transition pore complex (PTPC), the supramolecular entity that initiates MPT, remain to be elucidated. Specifically, the precise contribution o...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201643602
更新日期:2017-07-01 00:00:00
abstract::N6-methyladenosine (m6A) is a highly dynamic RNA modification that has recently emerged as a key regulator of gene expression. While many m6A modifications are installed by the METTL3-METTL14 complex, others appear to be introduced independently, implying that additional human m6A methyltransferases remain to be ident...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201744940
更新日期:2017-11-01 00:00:00
abstract::Microbes exert a major impact on human health and disease by either promoting or disrupting homeostasis, in the latter instance leading to the development of infectious diseases. Such disparate outcomes are driven by the ever-evolving genetic diversity of microbes and the countervailing host responses that minimize th...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.15252/embr.201540558
更新日期:2015-11-01 00:00:00
abstract::Nuclear-pore complexes (NPCs) are large protein channels that span the nuclear envelope (NE), which is a double membrane that encloses the nuclear genome of eukaryotes. Each of the typically 2,000-4,000 pores in the NE of vertebrate cells is composed of multiple copies of 30 different proteins known as nucleoporins. T...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.1038/embor.2009.147
更新日期:2009-07-01 00:00:00
abstract::The energetic metabolism of photosynthetic organisms is profoundly influenced by state transitions and cyclic electron flow around photosystem I. The former involve a reversible redistribution of the light-harvesting antenna between photosystem I and photosystem II and optimize light energy utilization in photosynthes...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1093/embo-reports/kvf047
更新日期:2002-03-01 00:00:00
abstract::The final irreversible step in the duplication and distribution of genomes to daughter cells takes place at the metaphase to anaphase transition. At this point aligned sister chromatid pairs split and separate. During metaphase, cohesion between sister chromatids is maintained by the chromosomal multi-subunit cohesin ...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.1093/embo-reports/kve113
更新日期:2001-06-01 00:00:00
abstract::Essential aspects of innate immune responses to microbial infections appear to be conserved between insects and mammals. In particular, in both groups, transmembrane receptors of the Toll superfamily play a crucial role in activating immune defenses. The Drosophila Toll family member 18-Wheeler had been proposed to se...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1093/embo-reports/kvf130
更新日期:2002-07-01 00:00:00
abstract::Somatic hypermutation of immunoglobulin variable genes, which increases antibody diversity, is initiated by the activation-induced cytosine deaminase (AID) protein. The current DNA-deamination model posits that AID deaminates cytosine to uracil in DNA, and that mutations are generated by DNA polymerases during replica...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.1038/sj.embor.7400582
更新日期:2005-12-01 00:00:00
abstract::The ubiquitin-like protein Urm1 can be covalently conjugated to other proteins, such as the yeast thioredoxin peroxidase protein Ahp1p, through a mechanism involving the ubiquitin E1-like enzyme Uba4. Recent findings have revealed a second function of Urm1 as a sulphur carrier in the thiolation of eukaryotic cytoplasm...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.1038/embor.2008.209
更新日期:2008-12-01 00:00:00
abstract::The imprint at the mat1 locus of Schizosaccharomyces pombe acts to initiate the replication-coupled recombination event that underlies mating-type switching. However, the nature of the imprint has been an area of dispute. Two alternative models have been proposed: one stated that the imprint is a nick in the DNA, wher...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400576
更新日期:2006-01-01 00:00:00
abstract::The orphan GluD2 receptor belongs to the ionotropic glutamate receptor family but does not bind glutamate. Ligand-gated GluD2 currents have never been evidenced, and whether GluD2 operates as an ion channel has been a long-standing question. Here, we show that GluD2 gating is triggered by type 1 metabotropic glutamate...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1002/embr.201337371
更新日期:2014-01-01 00:00:00
abstract::The ER-bound kinase/endoribonuclease (RNase), inositol-requiring enzyme-1 (IRE1), regulates the phylogenetically most conserved arm of the unfolded protein response (UPR). However, the complex biology and pathology regulated by mammalian IRE1 cannot be fully explained by IRE1's one known, specific RNA target, X box-bi...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.202051462
更新日期:2020-11-02 00:00:00
abstract::Activation of the elongation factor 2 kinase (eEF2K) leads to the phosphorylation and inhibition of the elongation factor eEF2, reducing mRNA translation rates. Emerging evidence indicates that the regulation of factors involved in protein synthesis may be critical for controlling diverse biological processes includin...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201642194
更新日期:2016-10-01 00:00:00
abstract::The delta2 glutamate receptor (GluRdelta2) has a crucial role in cerebellar functions; disruption of GluRdelta2 alleles in mice (delta2(-/-)) impairs synapse formation and long-term depression, which is thought to underlie motor learning in the cerebellum, and consequently leads to motor discoordination. However, it h...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400312
更新日期:2005-01-01 00:00:00
abstract::Recent work has brought to light many different mechanisms of translation initiation that function in cells in parallel to canonical cap-dependent initiation. This has important implications for cancer. Canonical cap-dependent translation initiation is inhibited by many stresses such as hypoxia, nutrient limitation, p...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.15252/embr.201845947
更新日期:2018-10-01 00:00:00
abstract::The primary cilium, critical for morphogenic and growth factor signaling, is assembled upon cell cycle exit, but the links between ciliogenesis and cell cycle progression are unclear. KV10.1 is a voltage-gated potassium channel frequently overexpressed in tumors. We have previously reported that expression of KV10.1 i...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201541082
更新日期:2016-05-01 00:00:00
abstract::Alternative RNA processing of LMNA pre-mRNA produces three main protein isoforms, that is, lamin A, progerin, and lamin C. De novo mutations that favor the expression of progerin over lamin A lead to Hutchinson-Gilford progeria syndrome (HGPS), providing support for the involvement of LMNA processing in pathological a...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1002/embr.201338126
更新日期:2014-05-01 00:00:00