RNA to the rescue: RNA is one of the most promising targets for drug development given its wide variety of uses.

abstract：:In Saccharomyces cerevisiae, genome stability depends on RNases H1 and H2, which remove ribonucleotides from DNA and eliminate RNA-DNA hybrids (R-loops). In Schizosaccharomyces pombe, RNase H enzymes were reported to process RNA-DNA hybrids produced at a double-strand break (DSB) generated by I-PpoI meganuclease. Howe...

journal_title：EMBO reports

authors： Zhao H,Zhu M,Limbo O,Russell P

更新日期：2018-05-01 00:00:00

abstract：:The developmental and oncogenic roles of MYC proteins are well established, but the transcriptional targets mediating their functions remain elusive. Using small interfering RNA-mediated knockdown in breast and cervix carcinoma cell lines, which overexpress c-MYC, we show that c-MYC independently controls metabolism a...

journal_title：EMBO reports

authors： Cappellen D,Schlange T,Bauer M,Maurer F,Hynes NE

更新日期：2007-01-01 00:00:00

abstract：:At gastrulation, a subpopulation of epiblast cells constitutes a transient posteriorly located structure called the primitive streak, where cells that undergo epithelial-mesenchymal transition make up the mesoderm and endoderm lineages. Mouse embryo epiblast cells were labelled ubiquitously or in a mosaic fashion. Cel...

journal_title：EMBO reports

authors： Mathiah N,Despin-Guitard E,Stower M,Nahaboo W,Eski ES,Singh SP,Srinivas S,Migeotte I

更新日期：2020-11-05 00:00:00

abstract：:gamma-Secretase-dependent regulated intramembrane proteolysis of amyloid precursor protein (APP) releases the APP intracellular domain (AICD). The question of whether this domain, like the Notch intracellular domain, is involved in nuclear signalling is highly controversial. Although some reports suggest that AICD reg...

journal_title：EMBO reports

authors： Hébert SS,Serneels L,Tolia A,Craessaerts K,Derks C,Filippov MA,Müller U,De Strooper B

更新日期：2006-07-01 00:00:00

abstract：:Although Niban is highly expressed in human cancer cells, the cellular functions of Niban remain largely unknown. We demonstrate here that ultraviolet irradiation induces phosphorylation of Niban at S602 by AKT, which increases the association of Niban with nucleophosmin and disassociation of nucleophosmin from the MD...

journal_title：EMBO reports

authors： Ji H,Ding Z,Hawke D,Xing D,Jiang BH,Mills GB,Lu Z

更新日期：2012-06-01 00:00:00

abstract：:The Drosophila immune system is able to discriminate between classes of bacteria. Detection of Gram-positive bacteria involves a complex of two pattern recognition receptors: peptidoglycan recognition protein SA (PGRP-SA) and Gram-negative binding protein 1 (GNBP1). These activate the Toll signalling pathway. To defin...

journal_title：EMBO reports

authors： Filipe SR,Tomasz A,Ligoxygakis P

更新日期：2005-04-01 00:00:00

abstract：:Sirtuins (SIRTs) are a class of lysine deacylases that regulate cellular metabolism and energy homeostasis. Although sirtuins have been proposed to function in nutrient sensing and signaling, the underlying mechanism remains elusive. SIRT7, a histone H3K18-specific deacetylase, epigenetically controls mitochondria bio...

journal_title：EMBO reports

authors： Yan WW,Liang YL,Zhang QX,Wang D,Lei MZ,Qu J,He XH,Lei QY,Wang YP

更新日期：2018-12-01 00:00:00

abstract：:Adenosine deaminase acting on RNA (ADAR)-catalyzed adenosine-to-inosine RNA editing is potentially dysregulated in neoplastic progression. However, how this transcriptome recoding process is functionally correlated with tumorigenesis remains largely elusive. Our analyses of RNA editome datasets identify hypoxia-relate...

journal_title：EMBO reports

authors： Ma CP,Liu H,Yi-Feng Chang I,Wang WC,Chen YT,Wu SM,Chen HW,Kuo YP,Shih CT,Li CY,Tan BC

更新日期：2019-05-01 00:00:00

abstract：:Ligand-dependent transcriptional activation of retinoic acid receptors (RARs) is a multistep process culminating in the formation of a multimeric co-activator complex on regulated promoters. Several co-activator complexes harbor an acetyl transferase activity, which is required for retinoid-induced transcription of re...

journal_title：EMBO reports

authors： Lefebvre B,Ozato K,Lefebvre P

更新日期：2002-04-01 00:00:00

abstract：:The female sex steroid hormone oestrogen stimulates both cell proliferation and cell differentiation in target tissues. These biological actions are mediated primarily through nuclear oestrogen receptors (ERs). The ligand-dependent transactivation of ERs requires several nuclear co-regulator complexes; however, the ce...

journal_title：EMBO reports

authors： Okada M,Takezawa S,Mezaki Y,Yamaoka I,Takada I,Kitagawa H,Kato S

更新日期：2008-06-01 00:00:00

abstract：:Human immunodeficiency virus 1 (HIV-1) expresses several accessory proteins that manipulate various host-cell processes to achieve optimum replicative efficiency. One of them, viral protein U (Vpu), has been shown to interfere with the cellular degradation machinery through interaction with SCF(beta-TrCP) complexes. T...

journal_title：EMBO reports

authors： Leulier F,Marchal C,Miletich I,Limbourg-Bouchon B,Benarous R,Lemaitre B

更新日期：2003-10-01 00:00:00

abstract：:The Lgr5 receptor is a marker of intestinal stem cells (ISCs) that regulates Wnt/b-catenin signaling. In this study, phenotype analysis of knockin/knockout Lgr5-eGFP-IRES-Cre and Lgr5-DTReGFP embryos reveals that Lgr5 deficiency during Wnt-mediated cytodifferentiation results in amplification of ISCs and early differe...

journal_title：EMBO reports

authors： Fernandez Vallone V,Leprovots M,Ribatallada-Soriano D,Gerbier R,Lefort A,Libert F,Vassart G,Garcia MI

更新日期：2020-07-03 00:00:00

abstract：:RNA silencing is a complex of mechanisms that regulate gene expression through small RNA molecules. The microRNA (miRNA) pathway is the most common of these in mammals. Genome-encoded miRNAs suppress translation in a sequence-specific manner and facilitate shifts in gene expression during developmental transitions. He...

journal_title：EMBO reports

authors： Svoboda P,Flemr M

更新日期：2010-08-01 00:00:00

abstract：:Elongation of the telomeric overhang by telomerase is counteracted by synthesis of the complementary strand by the CST complex, CTC1(Cdc13)/Stn1/Ten1. Interaction of budding yeast Stn1 with overhang-binding Cdc13 is increased by Cdc13 SUMOylation. Human and fission yeast CST instead interact with overhang-binding TPP1...

journal_title：EMBO reports

authors： Garg M,Gurung RL,Mansoubi S,Ahmed JO,Davé A,Watts FZ,Bianchi A

更新日期：2014-08-01 00:00:00

abstract：:The COP9 signalosome (CSN) is a highly conserved protein complex that was originally described as a repressor of light-dependent growth and transcription in Arabidopsis. The most studied CSN function is the regulation of protein degradation, which occurs primarily through the removal of the ubiquitin-like modifier Ned...

journal_title：EMBO reports

authors： Chamovitz DA

更新日期：2009-04-01 00:00:00

abstract：:Oncogenic signals contribute to enhanced glycolysis and mTORC1 activity, leading to rapid cell proliferation in cancer. Regulation of glycolysis and mTORC1 by PI3K/Akt signaling is well established, but how KRAS-induced MEK signaling regulates these pathways remains poorly understood. Here, we report a role for MEK-dr...

journal_title：EMBO reports

authors： Byun JK,Park M,Yun JW,Lee J,Kim JS,Cho SJ,Lee YM,Lee IK,Choi YK,Park KG

更新日期：2019-06-01 00:00:00

abstract：:GlpF, the glycerol facilitator protein of Escherichia coli, is an archetypal member of the aquaporin superfamily. To assess its structure, recombinant histidine-tagged protein was overexpressed, solubilized in octylglucoside and purified to homogeneity. Negative stain electron microscopy of solubilized GlpF protein re...

journal_title：EMBO reports

authors： Braun T,Philippsen A,Wirtz S,Borgnia MJ,Agre P,Kühlbrandt W,Engel A,Stahlberg H

更新日期：2000-08-01 00:00:00

abstract：:The hypoxic response in humans is mediated by the hypoxia-inducible transcription factor (HIF), for which prolyl hydroxylases (PHDs) act as oxygen-sensing components. The evolutionary origins of the HIF system have been previously unclear. We demonstrate a functional HIF system in the simplest animal, Trichoplax adhae...

journal_title：EMBO reports

authors： Loenarz C,Coleman ML,Boleininger A,Schierwater B,Holland PW,Ratcliffe PJ,Schofield CJ

更新日期：2011-01-01 00:00:00

abstract：:Histone deacetylases (HDACs)-an enzyme family that deacetylates histones and non-histone proteins-are implicated in human diseases such as cancer, and the first-generation of HDAC inhibitors are now in clinical trials. Here, we report the 2.0 A resolution crystal structure of a catalytically inactive HDAC8 active-site...

journal_title：EMBO reports

authors： Vannini A,Volpari C,Gallinari P,Jones P,Mattu M,Carfí A,De Francesco R,Steinkühler C,Di Marco S

更新日期：2007-09-01 00:00:00

abstract：:One limitation for the study of chromosomal fragile sites is that they must be studied on metaphase spreads, after the breakage. We show here that bacterial lac operator (lacO) repeats are prone to spontaneous breakage, which when combined with a fluorescent lac repressor (lacR) has allowed us to track a fragile site ...

journal_title：EMBO reports

authors： Jacome A,Fernandez-Capetillo O

更新日期：2011-09-30 00:00:00

abstract：:Various aerolysin-like pore-forming proteins have been identified from bacteria to vertebrates. However, the mechanism of receptor recognition and/or pore formation of the eukaryotic members remains unknown. Here, we present the first crystal and electron microscopy structures of a vertebrate aerolysin-like protein fr...

journal_title：EMBO reports

authors： Jia N,Liu N,Cheng W,Jiang YL,Sun H,Chen LL,Peng J,Zhang Y,Ding YH,Zhang ZH,Wang X,Cai G,Wang J,Dong MQ,Zhang Z,Wu H,Wang HW,Chen Y,Zhou CZ

更新日期：2016-02-01 00:00:00

abstract：:High expression levels of SLFN11 correlate with the sensitivity of human cancer cells to DNA-damaging agents. However, little is known about the underlying mechanism. Here, we show that SLFN11 interacts directly with RPA1 and is recruited to sites of DNA damage in an RPA1-dependent manner. Furthermore, we establish th...

journal_title：EMBO reports

authors： Mu Y,Lou J,Srivastava M,Zhao B,Feng XH,Liu T,Chen J,Huang J

更新日期：2016-01-01 00:00:00

abstract：:The tubby loci provide a unique opportunity to study adult-onset obesity. Mutation in either mammalian tubby or its homologue in Caenorhabditis elegans, tub-1, results in increased fat storage. Previously, we have shown that TUB-1 interacts with a new Rab GTPase-activating protein, RBG-3, for the regulation of fat sto...

journal_title：EMBO reports

authors： Mukhopadhyay A,Pan X,Lambright DG,Tissenbaum HA

更新日期：2007-10-01 00:00:00

abstract：:Cleavage of the cohesin subunit Scc1p/Mcd1p/Rad21 permits sister chromatid separation and is considered to trigger anaphase onset. It has also been suggested that the cohesin complex is essential for chromosome condensation and for assembling fully functional kinetochores. Here, we used vertebrate cells conditionally ...

journal_title：EMBO reports

authors： Vagnarelli P,Morrison C,Dodson H,Sonoda E,Takeda S,Earnshaw WC

更新日期：2004-02-01 00:00:00

abstract：:Loss of the retinoblastoma protein, pRB, leads to apoptosis, and several results have suggested that this is dependent on the E2F transcription factors. However, so far, the ability of the different E2F family members to contribute to apoptosis is controversial. Here, we show that ectopic expression of E2F3 results in...

journal_title：EMBO reports

authors： Lazzerini Denchi E,Helin K

更新日期：2005-07-01 00:00:00

abstract：:The angiotensin II type 1 (AT(1)) receptor is a G protein-coupled receptor that has a crucial role in the development of load-induced cardiac hypertrophy. Here, we show that cell stretch leads to activation of the AT(1) receptor, which undergoes an anticlockwise rotation and a shift of transmembrane (TM) 7 into the li...

journal_title：EMBO reports

authors： Yasuda N,Miura S,Akazawa H,Tanaka T,Qin Y,Kiya Y,Imaizumi S,Fujino M,Ito K,Zou Y,Fukuhara S,Kunimoto S,Fukuzaki K,Sato T,Ge J,Mochizuki N,Nakaya H,Saku K,Komuro I

更新日期：2008-02-01 00:00:00

abstract：:Embryonic stem cell (ESC) cultures display a heterogeneous gene expression profile, ranging from a pristine naïve pluripotent state to a primed epiblast state. Addition of inhibitors of GSK3β and MEK (so-called 2i conditions) pushes ESC cultures toward a more homogeneous naïve pluripotent state, but the molecular unde...

journal_title：EMBO reports

authors： Welling M,Chen HH,Muñoz J,Musheev MU,Kester L,Junker JP,Mischerikow N,Arbab M,Kuijk E,Silberstein L,Kharchenko PV,Geens M,Niehrs C,van de Velde H,van Oudenaarden A,Heck AJ,Geijsen N

更新日期：2015-07-01 00:00:00

abstract：:Most solid human tumours are aneuploid, that is, they contain an abnormal number of chromosomes. Paradoxically, however, aneuploidy has been reported to induce a stress response that suppresses cellular proliferation in vitro. Here, we review the progress in our understanding of the causes and effects of aneuploidy in...

journal_title：EMBO reports

authors： Holland AJ,Cleveland DW

更新日期：2012-06-01 00:00:00

abstract：:We have identified a novel component, Helicase89B, that is required for the inducible antimicrobial response in Drosophila larvae by means of a P-element insertional genetic screen. Helicase89B belongs to the Mot1p/BTAF1 subfamily of SNF2-like ATPases. This subfamily can interact with TATA-binding proteins, but whethe...

journal_title：EMBO reports

authors： Yagi Y,Ip YT

更新日期：2005-11-01 00:00:00

abstract：:Retinoic acid (RA) signalling ensures that vertebrate mesoderm segmentation is bilaterally synchronized, and corrects transient interferences from asymmetric left-right (L-R) signals involved in organ lateralization. Snail genes participate in both these processes and, although they are expressed symmetrically in the ...

journal_title：EMBO reports

authors： Morales AV,Acloque H,Ocaña OH,de Frutos CA,Gold V,Nieto MA

更新日期：2007-01-01 00:00:00