Abstract:
:The p53 tumour suppressor protein has a crucial role in cell-cycle arrest and apoptosis. Previous reports show that the p53 messenger RNA is translated to produce an amino-terminal-deleted isoform (DeltaN-p53) from an internal initiation codon, which acts as a dominant-negative inhibitor of full-length p53. Here, we show that two internal ribosome entry sites (IRESs) mediate the translation of both full-length and DeltaN-p53 isoforms. The IRES directing the translation of full-length p53 is in the 5'-untranslated region of the mRNA, whereas the IRES mediating the translation of DeltaN-p53 extends into the protein-coding region. The two IRESs show distinct cell-cycle phase-dependent activity, with the IRES for full-length p53 being active at the G2-M transition and the IRES for DeltaN-p53 showing highest activity at the G1-S transition. These results indicate a novel translational control of p53 gene expression and activity.
journal_name
EMBO Repjournal_title
EMBO reportsauthors
Ray PS,Grover R,Das Sdoi
10.1038/sj.embor.7400623keywords:
subject
Has Abstractpub_date
2006-04-01 00:00:00pages
404-10issue
4eissn
1469-221Xissn
1469-3178pii
7400623journal_volume
7pub_type
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