Secured cutting: controlling separase at the metaphase to anaphase transition.

Abstract:

:The final irreversible step in the duplication and distribution of genomes to daughter cells takes place at the metaphase to anaphase transition. At this point aligned sister chromatid pairs split and separate. During metaphase, cohesion between sister chromatids is maintained by the chromosomal multi-subunit cohesin complex. Here, I review recent findings as to how anaphase is initiated by proteolytic cleavage of the Scc1 subunit of cohesin. Scc1 is cleaved by a site-specific protease that is conserved in all eukaryotes, and is now called 'separase'. As a result of this cleavage, the cohesin complex is destroyed, allowing the spindle to pull sister chromatids into opposite halves of the cell. Because of the final and irreversible nature of Scc1 cleavage, this reaction is tightly controlled. Several independent mechanisms seem to impose regulation on Scc1 cleavage, acting on both the activity of separase and the susceptibility of the substrate.

journal_name

EMBO Rep

journal_title

EMBO reports

authors

Uhlmann F

doi

10.1093/embo-reports/kve113

keywords:

subject

Has Abstract

pub_date

2001-06-01 00:00:00

pages

487-92

issue

6

eissn

1469-221X

issn

1469-3178

pii

2/6/487

journal_volume

2

pub_type

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