Abstract:
:The final irreversible step in the duplication and distribution of genomes to daughter cells takes place at the metaphase to anaphase transition. At this point aligned sister chromatid pairs split and separate. During metaphase, cohesion between sister chromatids is maintained by the chromosomal multi-subunit cohesin complex. Here, I review recent findings as to how anaphase is initiated by proteolytic cleavage of the Scc1 subunit of cohesin. Scc1 is cleaved by a site-specific protease that is conserved in all eukaryotes, and is now called 'separase'. As a result of this cleavage, the cohesin complex is destroyed, allowing the spindle to pull sister chromatids into opposite halves of the cell. Because of the final and irreversible nature of Scc1 cleavage, this reaction is tightly controlled. Several independent mechanisms seem to impose regulation on Scc1 cleavage, acting on both the activity of separase and the susceptibility of the substrate.
journal_name
EMBO Repjournal_title
EMBO reportsauthors
Uhlmann Fdoi
10.1093/embo-reports/kve113keywords:
subject
Has Abstractpub_date
2001-06-01 00:00:00pages
487-92issue
6eissn
1469-221Xissn
1469-3178pii
2/6/487journal_volume
2pub_type
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