Abstract:
:Mini-chromosome maintenance (MCM) proteins form a conserved family found in all eukaryotes and are essential for DNA replication. They exist as heteromultimeric complexes containing as many as six different proteins. These complexes are believed to be the replicative helicases, functioning as hexameric rings at replication forks. In most archaea a single MCM protein exists. The protein from Methanobacterium thermoautotrophicum (mtMCM) has been reported to assemble into a large complex consistent with a dodecamer. We show that mtMCM can assemble into a heptameric ring. This ring contains a C-terminal helicase domain that can be fit with crystal structures of ring helicases and an N-terminal domain of unknown function. While the structure of the ring is very similar to that of hexameric replicative helicases such as bacteriophage T7 gp4, our results show that such ring structures may not be constrained to have only six subunits.
journal_name
EMBO Repjournal_title
EMBO reportsauthors
Yu X,VanLoock MS,Poplawski A,Kelman Z,Xiang T,Tye BK,Egelman EHdoi
10.1093/embo-reports/kvf160keywords:
subject
Has Abstractpub_date
2002-08-01 00:00:00pages
792-7issue
8eissn
1469-221Xissn
1469-3178pii
embor098journal_volume
3pub_type
杂志文章相关文献
EMBO REPORTS文献大全abstract::The ubiquitous inositol 1,4,5-trisphosphate receptor (InsP(3)R) intracellular Ca(2+) release channel is engaged by thousands of plasma membrane receptors to generate Ca(2+) signals in all cells. Understanding how complex Ca(2+) signals are generated has been hindered by a lack of information on the kinetic responses o...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7401087
更新日期:2007-11-01 00:00:00
abstract::When our time comes to die most people would probably opt for a quick, peaceful and painless exit. But the manner and timing are rarely under our direct control. Hence the Ars moriendi, literally, "The Art of Dying", two texts written in Latin around the 15th century that offered advice on how to die well according to...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.1002/embr.201337970
更新日期:2014-02-01 00:00:00
abstract::Given that ligand binding is essential for the rapid internalization of epidermal growth factor receptor (EGFR), the events induced by ligand binding probably contribute to the regulation of EGFR internalization. These events include receptor dimerization, activation of intrinsic tyrosine kinase activity and autophosp...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400491
更新日期:2005-10-01 00:00:00
abstract::In mammals, immediate-early transcription of the Period 1 (Per1) gene is crucial for resetting the mammalian circadian clock. Here, we show that CLOCK is a real signalling molecule that mediates the serum-evoked rapid induction of Per1 in fibroblasts through the Ca2+-dependent protein kinase C (PKC) pathway. Stimulati...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400920
更新日期:2007-04-01 00:00:00
abstract::During the past years, yeasts have been successfully established as models to study the mechanisms of apoptotic regulation. We recently showed that mutations in the LSM4 gene, which is involved in messenger RNA decapping, lead to increased mRNA stability and apoptosis in yeast. Here, we show that mitochondrial functio...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400514
更新日期:2005-11-01 00:00:00
abstract::The imprint at the mat1 locus of Schizosaccharomyces pombe acts to initiate the replication-coupled recombination event that underlies mating-type switching. However, the nature of the imprint has been an area of dispute. Two alternative models have been proposed: one stated that the imprint is a nick in the DNA, wher...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400576
更新日期:2006-01-01 00:00:00
abstract::The Nedd4 family E3 ligases are key regulators of cell growth and proliferation and are often misregulated in human cancers and other diseases. The ligase activities of Nedd4 E3s are tightly controlled via auto-inhibition. However, the molecular mechanism underlying Nedd4 E3 auto-inhibition and activation is poorly un...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201744454
更新日期:2017-09-01 00:00:00
abstract::RNA modifications represent a novel layer of regulation of gene expression. Functional experiments revealed that N6 -methyladenosine (m6 A) on messenger RNA (mRNA) plays critical roles in cell fate determination and development. m6 A mark also resides in the decoding center of 18S ribosomal RNA (rRNA); however, the bi...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201949863
更新日期:2020-10-05 00:00:00
abstract::Senescence is a stable proliferative arrest induced by various stresses such as telomere erosion, oncogenic or oxidative stress. Compelling evidence suggests that it acts as a barrier against tumour development. Describing new mechanisms that favour an escape from senescence can thus reveal new insights into tumorigen...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2008.255
更新日期:2009-03-01 00:00:00
abstract::Research into the extreme feats of endurance that humans and some animals achieve has uncovered a range of metabolic and physiological adaptations along with their evolutionary origins. ...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.15252/embr.201949396
更新日期:2019-11-05 00:00:00
abstract::The hypoxic response in humans is mediated by the hypoxia-inducible transcription factor (HIF), for which prolyl hydroxylases (PHDs) act as oxygen-sensing components. The evolutionary origins of the HIF system have been previously unclear. We demonstrate a functional HIF system in the simplest animal, Trichoplax adhae...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2010.170
更新日期:2011-01-01 00:00:00
abstract::Defects in protein folding and the proteasomal pathway have been linked with many neurodegenerative diseases. PLIC-1 (protein linking IAP to the cytoskeleton) is a ubiquitin-like protein that binds to the ubiquitin-interacting motif (UIM) of the proteasomal subunit S5a. Here, we show that PLIC-1 also binds to the UIM ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400823
更新日期:2006-12-01 00:00:00
abstract::Pair-rule genes in Drosophila integrate the positional information provided by the transcription factor gradients of gap genes and set the framework for the expression of the segment polarity genes. Pair-rule genes are also expressed during the segmentation of the short germ-band embryo of the flour beetle Tribolium, ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400148
更新日期:2004-06-01 00:00:00
abstract::PTIP regulates gene transcription by controlling the methylation of histone H3, and also has important roles in cellular responses to DNA damage or to perturbed DNA replication. The available data suggest that the functions of PTIP in transcription and preserving genome stability might be independent and mediated by f...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.1038/embor.2009.21
更新日期:2009-03-01 00:00:00
abstract::Retinoic acid (RA) signalling ensures that vertebrate mesoderm segmentation is bilaterally synchronized, and corrects transient interferences from asymmetric left-right (L-R) signals involved in organ lateralization. Snail genes participate in both these processes and, although they are expressed symmetrically in the ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400867
更新日期:2007-01-01 00:00:00
abstract::A variety of nuclear localization signals (NLSs) are experimentally known although only one motif was available for database searches through PROSITE. We initially collected a set of 91 experimentally verified NLSs from the literature. Through iterated 'in silico mutagenesis' we then extended the set to 214 potential ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1093/embo-reports/kvd092
更新日期:2000-11-01 00:00:00
abstract::Paxillin is a protein containing four LIM domains, and functions in integrin signaling. We report here that two transcripts are generated from the paxillin gene locus in Drosophila; one encodes a protein homolog of the vertebrate Paxillin (DPxn37), and the other a protein with only three LIM domains, partly encoded by...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1093/embo-reports/kve178
更新日期:2001-09-01 00:00:00
abstract::Howy Jacobs reminisces about his many editorials and asks whether they have ever been read by anyone. ...
journal_title:EMBO reports
pub_type: 社论
doi:10.15252/embr.202050838
更新日期:2020-07-03 00:00:00
abstract::We have identified a novel component, Helicase89B, that is required for the inducible antimicrobial response in Drosophila larvae by means of a P-element insertional genetic screen. Helicase89B belongs to the Mot1p/BTAF1 subfamily of SNF2-like ATPases. This subfamily can interact with TATA-binding proteins, but whethe...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400542
更新日期:2005-11-01 00:00:00
abstract::Many human diseases owe their pathology, to some degree, to the erroneous conversion of proteins from their soluble state into fibrillar, β-structured aggregates, often referred to as amyloid fibrils. Neurodegenerative diseases, such as Alzheimer and spongiform encephalopathies, as well as type 2 diabetes and both loc...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.1038/embor.2011.116
更新日期:2011-07-01 00:00:00
abstract::The major DNA methyltransferase, Dnmt1, associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand. In view of the slow kinetics of Dnmt1 in vitro versus the fast progression of the replication fork, we have tested whether Dnmt1 associates with chromatin beyond...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400295
更新日期:2004-12-01 00:00:00
abstract::Small non-coding miRNA appear to be vital in brain development and function by organising complex patterns of gene expression. These molecules are important for the regulation of synaptically localised mRNAs that encode proteins involved in neurotransmission and behaviour. In this issue of EMBO Reports, Lackinger et a...
journal_title:EMBO reports
pub_type: 评论,杂志文章
doi:10.15252/embr.201947663
更新日期:2019-02-01 00:00:00
abstract::Cleavage of the cohesin subunit Scc1p/Mcd1p/Rad21 permits sister chromatid separation and is considered to trigger anaphase onset. It has also been suggested that the cohesin complex is essential for chromosome condensation and for assembling fully functional kinetochores. Here, we used vertebrate cells conditionally ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400077
更新日期:2004-02-01 00:00:00
abstract::Ageing is an unavoidable corollary to being alive; the most intuitive interpretation of ageing being that it is the consequence of progressive body degeneration. In agreement with this, current models propose that ageing occurs through a stepwise accumulation of DNA damage, which ultimately limits the regenerative cap...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2009.262
更新日期:2010-01-01 00:00:00
abstract::Comment on "Close loop peer review" by Michael Hill. ...
journal_title:EMBO reports
pub_type: 评论,信件
doi:10.15252/embr.201948313
更新日期:2019-08-01 00:00:00
abstract::There is a great interest in determining the impact of p53 on ageing and, for this, it is important to discriminate among the known causes of ageing. Telomere loss is a well-established source of age-associated damage, which by itself can recapitulate ageing in mouse models. Here, we have used a genetic approach to in...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400667
更新日期:2006-05-01 00:00:00
abstract::Alternative RNA processing of LMNA pre-mRNA produces three main protein isoforms, that is, lamin A, progerin, and lamin C. De novo mutations that favor the expression of progerin over lamin A lead to Hutchinson-Gilford progeria syndrome (HGPS), providing support for the involvement of LMNA processing in pathological a...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1002/embr.201338126
更新日期:2014-05-01 00:00:00
abstract::Mitochondrial respiration generates an electrochemical proton gradient across the mitochondrial inner membrane called protonmotive force (PMF) to drive diverse functions and synthesize ATP. Current techniques to manipulate the PMF are limited to its dissipation; yet, there is no precise and reversible method to increa...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201949113
更新日期:2020-04-03 00:00:00
abstract::Miro is a highly conserved calcium-binding GTPase at the regulatory nexus of mitochondrial transport and autophagy. Here we present crystal structures comprising the tandem EF hand and carboxy terminal GTPase (cGTPase) domains of Drosophila Miro. The structures reveal two previously unidentified 'hidden' EF hands, eac...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2013.151
更新日期:2013-11-01 00:00:00
abstract::Stat1 (signal transducer and activator of transcription 1) regulates transcription in response to the type I interferons IFN-alpha and IFN-beta, either in its dimerized form or as a subunit of the interferon-stimulated gene factor 3 (Isgf3) complex (consisting of Stat1, Stat2 and interferon-regulating factor 9). Full-...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.embor802
更新日期:2003-04-01 00:00:00