Abstract:
:During the past years, yeasts have been successfully established as models to study the mechanisms of apoptotic regulation. We recently showed that mutations in the LSM4 gene, which is involved in messenger RNA decapping, lead to increased mRNA stability and apoptosis in yeast. Here, we show that mitochondrial function and YCA1, which encodes a budding yeast metacaspase, are necessary for apoptosis triggered by stabilization of mRNAs. Deletion of YCA1 in yeast cells mutated in the LSM4 gene prevents mitochondrial fragmentation and rapid cell death during chronological ageing of the culture, diminishes reactive oxygen species accumulation and DNA breakage, and increases resistance to H2O2 and acetic acid. mRNA levels in lsm4 mutants deleted for YCA1 are still increased, positioning the Yca1 budding yeast caspase as a downstream executor of cell death induced by mRNA perturbations. In addition, we show that mitochondrial function is necessary for fast death during chronological ageing, as well as in LSM4 mutated and wild-type cells.
journal_name
EMBO Repjournal_title
EMBO reportsauthors
Mazzoni C,Herker E,Palermo V,Jungwirth H,Eisenberg T,Madeo F,Falcone Cdoi
10.1038/sj.embor.7400514keywords:
subject
Has Abstractpub_date
2005-11-01 00:00:00pages
1076-81issue
11eissn
1469-221Xissn
1469-3178pii
7400514journal_volume
6pub_type
杂志文章相关文献
EMBO REPORTS文献大全abstract::Hypoxic and oxidant stresses can coexist in biological systems, and oxidant stress has been proposed to activate hypoxia pathways through the inactivation of the 'oxygen-sensing' hypoxia-inducible factor (HIF) prolyl and asparaginyl hydroxylases. Here, we show that despite reduced sensitivity to cellular hypoxia, the ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2012.9
更新日期:2012-03-01 00:00:00
abstract::Cardiac dysfunctions dramatically increase with age. Revealing a currently unknown contributor to cardiac ageing, we report the age-dependent, cardiac-specific accumulation of the lysosphingolipid sphinganine (dihydrosphingosine, DHS) as an evolutionarily conserved hallmark of the aged vertebrate heart. Mechanisticall...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201847407
更新日期:2019-04-01 00:00:00
abstract::DNA repair by homologous recombination is involved in maintaining genome stability. Previous data report that wild-type p53 suppresses homologous recombination and physically interacts with Rad51. Here, we show the in vivo binding of wild-type p53 to a p53 response element in the promoter of Rad51 and the downregulati...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400587
更新日期:2006-02-01 00:00:00
abstract::Codon bias has been implicated as one of the major factors contributing to mRNA stability in several model organisms. However, the molecular mechanisms of codon bias on mRNA stability remain unclear in humans. Here, we show that human cells possess a mechanism to modulate RNA stability through a unique codon bias. Bio...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201948220
更新日期:2019-11-05 00:00:00
abstract::Acetaminophen/paracetamol-induced liver failure--which is induced by the binding of reactive metabolites to mitochondrial proteins and their disruption--is exacerbated by fasting. As fasting promotes SIRT3-mediated mitochondrial-protein deacetylation and acetaminophen metabolites bind to lysine residues, we investigat...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2011.121
更新日期:2011-07-01 00:00:00
abstract::The race for a vaccine against SARS-CoV-2 has accelerated research on RNA-based therapeutics. Beyond vaccines, RNA also shows great potential for cancer therapies. ...
journal_title:EMBO reports
pub_type: 评论,杂志文章
doi:10.15252/embr.202051013
更新日期:2020-07-03 00:00:00
abstract::The natural progressive dysfunction of most living organisms-ageing-has captured the attention of several studies, with the intention to develop rejuvenation strategies. Evidence is emerging of a positive correlation between natural ageing and chromosomal instability (CIN). In this issue of EMBO Reports, Barroso-Vilar...
journal_title:EMBO reports
pub_type: 评论,新闻
doi:10.15252/embr.202050322
更新日期:2020-05-06 00:00:00
abstract::Myc is an oncoprotein transcription factor that promotes cell proliferation and apoptosis. Analysis of highly conserved elements within vertebrate Myc proteins has been instrumental in defining the functions of the Myc protein. Here, we probe the role of a highly conserved, but little studied, element within the centr...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400333
更新日期:2005-02-01 00:00:00
abstract::Senescence is a stable proliferative arrest induced by various stresses such as telomere erosion, oncogenic or oxidative stress. Compelling evidence suggests that it acts as a barrier against tumour development. Describing new mechanisms that favour an escape from senescence can thus reveal new insights into tumorigen...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2008.255
更新日期:2009-03-01 00:00:00
abstract::Spatio-temporal regulation of signalling pathways plays a key role in generating diverse responses during the development of multicellular organisms. The role of signal dynamics in transferring signalling information in vivo is incompletely understood. Here, we employ genome engineering in Drosophila melanogaster to g...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201947999
更新日期:2019-12-05 00:00:00
abstract::The cellular response to DNA breaks is influenced by chromatin compaction. To identify chromatin regulators involved in the DNA damage response, we screened for genes that affect recovery following DNA damage using an RNAi library of chromatin regulators. We identified genes involved in chromatin remodeling, sister ch...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201948460
更新日期:2020-01-07 00:00:00
abstract::Loss of the retinoblastoma protein, pRB, leads to apoptosis, and several results have suggested that this is dependent on the E2F transcription factors. However, so far, the ability of the different E2F family members to contribute to apoptosis is controversial. Here, we show that ectopic expression of E2F3 results in...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400452
更新日期:2005-07-01 00:00:00
abstract::The tubby loci provide a unique opportunity to study adult-onset obesity. Mutation in either mammalian tubby or its homologue in Caenorhabditis elegans, tub-1, results in increased fat storage. Previously, we have shown that TUB-1 interacts with a new Rab GTPase-activating protein, RBG-3, for the regulation of fat sto...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7401055
更新日期:2007-10-01 00:00:00
abstract::The cytosolic pathogen sensor RIG-I is activated by RNAs with exposed 5'-triphosphate (5'-ppp) and terminal double-stranded structures, such as those that are generated during viral infection. RIG-I has been shown to translocate on dsRNA in an ATP-dependent manner. However, the precise role of the ATPase activity in R...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2013.102
更新日期:2013-09-01 00:00:00
abstract::Regulated intramembrane proteolysis of the amyloid precursor-protein (APP) produces both a characterstic amyloid-β peptide that contributes to neuritic plaque formation and neurodegeneration in Alzheimer disease and a small APP intracellular domain (AICD) that transcriptionally activates genes implicated in Alzheimer ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2010.210
更新日期:2011-03-01 00:00:00
abstract::Oncogenic signals contribute to enhanced glycolysis and mTORC1 activity, leading to rapid cell proliferation in cancer. Regulation of glycolysis and mTORC1 by PI3K/Akt signaling is well established, but how KRAS-induced MEK signaling regulates these pathways remains poorly understood. Here, we report a role for MEK-dr...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201847451
更新日期:2019-06-01 00:00:00
abstract::Mitochondria play a central role in stem cell homeostasis. Reversible switching between aerobic and anaerobic metabolism is critical for stem cell quiescence, multipotency, and differentiation, as well as for cell reprogramming. However, the effect of mitochondrial dysfunction on neural stem cell (NSC) function is uns...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201540982
更新日期:2015-11-01 00:00:00
abstract::Plasma membrane PI4P is an important direct regulator of many processes that occur at the plasma membrane and also a biosynthetic precursor of PI(4,5)P2 and its downstream metabolites. The majority of this PI4P pool is synthesized by an evolutionarily conserved complex, which has as its core the PI 4-kinase PI4KIIIα (...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201439151
更新日期:2015-03-01 00:00:00
abstract::Cortical pulling forces on astral microtubules are essential to position the spindle. These forces are generated by cortical dynein, a minus-end directed motor. Previously, another dynein regulator termed Spindly was proposed to regulate dynein-dependent spindle positioning. However, the mechanism of how Spindly regul...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201541143
更新日期:2016-03-01 00:00:00
abstract::Uropathogenic Escherichia coli (UPEC) is the primary cause of symptomatic urinary tract infection. The P-pili, a bacterial surface organelle, mediates the bacterial host--cell adhesion. The PapG adhesin has generated much interest in recent years, not only because of its clinical value, i.e. in the prevention of micro...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1093/embo-reports/kve133
更新日期:2001-07-01 00:00:00
abstract::Scrapie, bovine spongiform encephalopathy and chronic wasting disease are orally communicable, transmissible spongiform encephalopathies (TSEs). As zoonotic transmissions of TSE agents may pose a risk to human health, the identification of reservoirs for infectivity in animal tissues and their exclusion from human con...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.embor827
更新日期:2003-05-01 00:00:00
abstract::The histone H3 N-terminal protein domain (N-tail) is regulated by multiple posttranslational modifications, including methylation, acetylation, phosphorylation, and by proteolytic cleavage. However, the mechanism underlying H3 N-tail proteolytic cleavage is largely elusive. Here, we report that JMJD5, a Jumonji C (Jmj...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201743892
更新日期:2017-12-01 00:00:00
abstract::The accuracy of large-scale DNA sequencing is difficult to estimate without redundant effort. We have found that the mobile genetic element IS10, a component of the transposon Tn10, has contaminated a significant number of clones in the public databases, as a result of the use of the transposon in bacterial cloning st...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1093/embo-reports/kvd015
更新日期:2000-07-01 00:00:00
abstract::The role of cytochrome c (Cyt c) in caspase activation has largely been established from mammalian cell-culture studies, but much remains to be learned about its physiological relevance in situ. The role of Cyt c in invertebrates has been subject to considerable controversy. The Drosophila genome contains distinct cyt...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400773
更新日期:2006-09-01 00:00:00
abstract::Plexins are receptors for the axonal guidance molecules known as semaphorins, and the semaphorin 4D (Sema4D) receptor plexin-B1 induces repulsive responses by functioning as an R-Ras GTPase-activating protein (GAP). Here we characterized the downstream signalling of plexin-B1-mediated R-Ras GAP activity, inducing grow...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400737
更新日期:2006-07-01 00:00:00
abstract::The highly homologous Cl(-) channels CLC-Ka and CLC-Kb are important for water and salt conservation in the kidney and for the production of endolymph in the inner ear. Mutations in CLC-Kb lead to Bartter's syndrome and mutations in the small CLC-K subunit barttin lead to Bartter's syndrome and deafness. Here we show ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400169
更新日期:2004-06-01 00:00:00
abstract::Parkin, a product of the causative gene of autosomal-recessive juvenile parkinsonism (AR-JP), is a RING-type E3 ubiquitin ligase and has an amino-terminal ubiquitin-like (Ubl) domain. Although a single mutation that causes an Arg to Pro substitution at position 42 of the Ubl domain (the Arg 42 mutation) has been ident...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.embor764
更新日期:2003-03-01 00:00:00
abstract::In yeast, three small nucleolar RNAs (snoRNAs) are essential for the processing of pre-ribosomal RNA--U3, U14 and snR30--whereas 72 non-essential snoRNAs direct site-specific modification of pre-rRNA. We applied a quantitative screen for alterations in the pre-ribosome association to all 75 yeast snoRNAs in strains de...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2008.184
更新日期:2008-12-01 00:00:00
abstract::The p53 tumour suppressor protein has a crucial role in cell-cycle arrest and apoptosis. Previous reports show that the p53 messenger RNA is translated to produce an amino-terminal-deleted isoform (DeltaN-p53) from an internal initiation codon, which acts as a dominant-negative inhibitor of full-length p53. Here, we s...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400623
更新日期:2006-04-01 00:00:00
abstract::It is widely accepted that the large trinucleotide repeat expansions observed in many neurological diseases occur during replication. However, genetic recombination has emerged as a major source of instability for tandem repeats, including minisatellites, and recent studies raise the possibility that it may also be re...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.1093/embo-reports/kvd031
更新日期:2000-08-01 00:00:00