Abstract:
:The ubiquitous inositol 1,4,5-trisphosphate receptor (InsP(3)R) intracellular Ca(2+) release channel is engaged by thousands of plasma membrane receptors to generate Ca(2+) signals in all cells. Understanding how complex Ca(2+) signals are generated has been hindered by a lack of information on the kinetic responses of the channel to its primary ligands, InsP(3) and Ca(2+), which activate and inhibit channel gating. Here, we describe the kinetic responses of single InsP(3)R channels in native endoplasmic reticulum membrane to rapid ligand concentration changes with millisecond resolution, using a new patch-clamp configuration. The kinetics of channel activation and deactivation showed novel Ca(2+) regulation and unexpected ligand cooperativity. The kinetics of Ca(2+)-mediated channel inhibition showed the single-channel bases for fundamental Ca(2+) release events and Ca(2+) release refractory periods. These results provide new insights into the channel regulatory mechanisms that contribute to complex spatial and temporal features of intracellular Ca(2+) signals.
journal_name
EMBO Repjournal_title
EMBO reportsauthors
Mak DO,Pearson JE,Loong KP,Datta S,Fernández-Mongil M,Foskett JKdoi
10.1038/sj.embor.7401087subject
Has Abstractpub_date
2007-11-01 00:00:00pages
1044-51issue
11eissn
1469-221Xissn
1469-3178pii
7401087journal_volume
8pub_type
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