Abstract:
:The major DNA methyltransferase, Dnmt1, associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand. In view of the slow kinetics of Dnmt1 in vitro versus the fast progression of the replication fork, we have tested whether Dnmt1 associates with chromatin beyond S phase. Using time-lapse microscopy of mammalian cells expressing green-fluorescent-protein-tagged Dnmt1 and DsRed-tagged DNA Ligase I as a cell cycle progression marker, we have found that Dnmt1 associates with chromatin during G2 and M. This association is mediated by a specific targeting sequence, shows strong preference for constitutive but not facultative heterochromatin and is independent of heterochromatin-specific histone H3 Lys 9 trimethylation, SUV39H and HP1. Moreover, photobleaching analyses showed that Dnmt1 is continuously loaded onto chromatin throughout G2 and M, indicating a replication-independent role of Dnmt1 that could represent a novel and separate pathway to maintain DNA methylation.
journal_name
EMBO Repjournal_title
EMBO reportsauthors
Easwaran HP,Schermelleh L,Leonhardt H,Cardoso MCdoi
10.1038/sj.embor.7400295keywords:
subject
Has Abstractpub_date
2004-12-01 00:00:00pages
1181-6issue
12eissn
1469-221Xissn
1469-3178pii
7400295journal_volume
5pub_type
杂志文章相关文献
EMBO REPORTS文献大全abstract::Individuals affected by the autosomal recessive disorder Werner's syndrome (WS) develop many of the symptoms characteristic of premature ageing. Primary fibroblasts cultured from WS patients exhibit karyotypic abnormalities and a reduced replicative life span. The WRN gene encodes a 3'-5' DNA helicase, and is a member...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1093/embo-reports/kvd004
更新日期:2000-07-01 00:00:00
abstract::Paxillin is a protein containing four LIM domains, and functions in integrin signaling. We report here that two transcripts are generated from the paxillin gene locus in Drosophila; one encodes a protein homolog of the vertebrate Paxillin (DPxn37), and the other a protein with only three LIM domains, partly encoded by...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1093/embo-reports/kve178
更新日期:2001-09-01 00:00:00
abstract::Histone demethylases-both lysine-specific demethylase 1 (LSD1) and Jumonji-C (JmjC) domain-containing proteins-are broadly implicated in the regulation of chromatin-dependent processes. In Arabidopsis thaliana, histone marks directly affect DNA methylation, and mutations in LSD1 homologues show reduced DNA methylation...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2010.158
更新日期:2010-12-01 00:00:00
abstract::The intracellular modification of proteins by the addition of a single O-linked N-acetylglucosamine (O-GlcNAc) molecule is a ubiquitous post-translational modification in eukaryotic cells. It is catalysed by O-linked N-acetylglucosaminyltransferase, which attaches O-GlcNAc to serine/threonine residues, and it is count...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.1038/embor.2008.129
更新日期:2008-08-01 00:00:00
abstract::The thymus provides a specialized microenvironment in which distinct subsets of thymic epithelial cells (TECs) support T-cell development. Here, we describe the significance of cortical TECs (cTECs) in T-cell development, using a newly established mouse model of cTEC deficiency. The deficiency of mature cTECs caused a...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201540096
更新日期:2015-05-01 00:00:00
abstract::The role of cytochrome c (Cyt c) in caspase activation has largely been established from mammalian cell-culture studies, but much remains to be learned about its physiological relevance in situ. The role of Cyt c in invertebrates has been subject to considerable controversy. The Drosophila genome contains distinct cyt...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400773
更新日期:2006-09-01 00:00:00
abstract::The Vav family of proteins are guanine nucleotide exchange factors (GEFs) for the Rho family of GTPases, which regulate various cellular functions, including T-cell activation. They contain a catalytic Dbl homology (DH) domain that is invariably followed by a pleckstrin homology (PH) domain, which is often required fo...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2008.80
更新日期:2008-07-01 00:00:00
abstract::Foxp3(+) regulatory T cells (Tregs) exhibit plasticity, which dictates their function. Secretion of the inflammatory cytokine IFNγ, together with the acquisition of a T helper 1 (Th1)-like effector phenotype as observed in cancer, infection, and autoimmune diseases, is associated with loss of Treg suppressor function ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201541905
更新日期:2016-08-01 00:00:00
abstract::The Carma1-Bcl10-Malt1 (CBM) complex connects T-cell receptor (TCR) signalling to the canonical IkappaB kinase (IKK)/NF (nuclear factor)-kappaB pathway. Earlier studies have indicated that the COP9 signalosome (CSN), a pleiotropic regulator of the ubiquitin/26S proteasome system, controls antigen responses in T cells....
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2009.64
更新日期:2009-06-01 00:00:00
abstract::Oncogenic signals contribute to enhanced glycolysis and mTORC1 activity, leading to rapid cell proliferation in cancer. Regulation of glycolysis and mTORC1 by PI3K/Akt signaling is well established, but how KRAS-induced MEK signaling regulates these pathways remains poorly understood. Here, we report a role for MEK-dr...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201847451
更新日期:2019-06-01 00:00:00
abstract::Tie2, an endothelial cell-specific receptor kinase, has an important role in tumour angiogenesis. In an attempt to identify peptides that specifically interact with and block the Tie2 pathway, a phage-displayed peptide library was screened on a recombinant Tie2 receptor. One peptide, NLLMAAS, completely abolished the ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400100
更新日期:2004-03-01 00:00:00
abstract::The cytosolic pathogen sensor RIG-I is activated by RNAs with exposed 5'-triphosphate (5'-ppp) and terminal double-stranded structures, such as those that are generated during viral infection. RIG-I has been shown to translocate on dsRNA in an ATP-dependent manner. However, the precise role of the ATPase activity in R...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2013.102
更新日期:2013-09-01 00:00:00
abstract::The mechanisms that regulate the transcription of the tumour suppressor genes p53 and IRF-1 are poorly understood. We have characterized a 68-kDa transcription factor, GAAP-1 (gatekeeper of apoptosis activating proteins), encoded by an alternative splice product of the PRDII-BF1 gene, that recognizes a novel regulator...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1093/embo-reports/kvf032
更新日期:2002-02-01 00:00:00
abstract::Epidermal growth factor (EGF) activates the EGF receptor (EGFR) and stimulates its internalization and trafficking to lysosomes for degradation. However, a percentage of EGFR undergoes ligand-independent endocytosis and is rapidly recycled back to the plasma membrane. Importantly, alterations in EGFR recycling are a c...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201744034
更新日期:2017-12-01 00:00:00
abstract::Immune signaling networks must be tunable to alleviate fitness costs associated with immunity and, at the same time, robust against pathogen interferences. How these properties mechanistically emerge in plant immune signaling networks is poorly understood. Here, we discovered a molecular mechanism by which the model p...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201643051
更新日期:2017-03-01 00:00:00
abstract::Mitochondrial respiration generates an electrochemical proton gradient across the mitochondrial inner membrane called protonmotive force (PMF) to drive diverse functions and synthesize ATP. Current techniques to manipulate the PMF are limited to its dissipation; yet, there is no precise and reversible method to increa...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201949113
更新日期:2020-04-03 00:00:00
abstract::Biasing the sex ratio of populations of different organisms, including plants, insects, crustacean, and fish, has been demonstrated by genetic and non-genetic approaches. However, biasing the sex ratio of mammalian populations has not been demonstrated genetically. Here, we provide a first proof of concept for such a ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201948269
更新日期:2019-08-01 00:00:00
abstract::We have designed a doxycycline-regulated form of the H1 promoter of RNA polymerase III that allows the inducible knockdown of gene expression by small interfering RNAs (siRNAs). As a proof-of-principle, we have targeted beta-catenin in colorectal cancer (CRC) cells. T-cell factor (TCF) target-gene expression is induce...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.embor865
更新日期:2003-06-01 00:00:00
abstract::HOIL-1L and its binding partner HOIP are essential components of the E3-ligase complex that generates linear ubiquitin (Ub) chains, which are critical regulators of NF-κB activation. Using crystallographic and mutational approaches, we characterize the unexpected structural basis for the specific interaction between t...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2012.24
更新日期:2012-05-01 00:00:00
abstract::Hydrogen sulfide (H2 S), an endogenous signaling gaseous molecule, is involved in various physiological activities, including vessel relaxation, regulation of cellular bioenergetics, inflammation, and angiogenesis. By using xenograft orthotopic implantation of prostate cancer PC3 cells and subsequently comparing bone ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201845986
更新日期:2019-10-04 00:00:00
abstract::Acetaminophen/paracetamol-induced liver failure--which is induced by the binding of reactive metabolites to mitochondrial proteins and their disruption--is exacerbated by fasting. As fasting promotes SIRT3-mediated mitochondrial-protein deacetylation and acetaminophen metabolites bind to lysine residues, we investigat...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2011.121
更新日期:2011-07-01 00:00:00
abstract::PTIP regulates gene transcription by controlling the methylation of histone H3, and also has important roles in cellular responses to DNA damage or to perturbed DNA replication. The available data suggest that the functions of PTIP in transcription and preserving genome stability might be independent and mediated by f...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.1038/embor.2009.21
更新日期:2009-03-01 00:00:00
abstract::IQGAP scaffold proteins are evolutionarily conserved in eukaryotes and facilitate the formation of complexes that regulate cytoskeletal dynamics, intracellular signaling, and intercellular interactions. Fungal and mammalian IQGAPs are implicated in cytokinesis. IQGAP1, IQGAP2, and IQGAP3 have diverse roles in vertebra...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.15252/embr.201439834
更新日期:2015-04-01 00:00:00
abstract::In response to a variety of stresses, mammalian cells undergo a persistent proliferative arrest known as cellular senescence. Many senescence-inducing stressors are potentially oncogenic, strengthening the notion that senescence evolved alongside apoptosis to suppress tumorigenesis. In contrast to apoptosis, senescent...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.15252/embr.201439245
更新日期:2014-11-01 00:00:00
abstract::Vascular endothelial growth factor (VEGF) is the master regulator of angiogenesis, whose best-understood mechanism is sprouting. However, therapeutic VEGF delivery to ischemic muscle induces angiogenesis by the alternative process of intussusception, or vascular splitting, whose molecular regulation is essentially unk...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201745054
更新日期:2018-05-01 00:00:00
abstract::Initiation of DNA replication from the Escherichia coli chromosomal origin is highly regulated, assuring that replication occurs precisely once per cell cycle. Three mechanisms for regulation of replication initiation have been proposed: titration of free DnaA initiator protein by the datA locus, sequestration of newl...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400467
更新日期:2005-08-01 00:00:00
abstract::The final irreversible step in the duplication and distribution of genomes to daughter cells takes place at the metaphase to anaphase transition. At this point aligned sister chromatid pairs split and separate. During metaphase, cohesion between sister chromatids is maintained by the chromosomal multi-subunit cohesin ...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.1093/embo-reports/kve113
更新日期:2001-06-01 00:00:00
abstract::Dynactin has been proposed to link the microtubule-associated motor cytoplasmic dynein with membranous cargo; however, the mechanism by which dynactin-membrane interaction is regulated is unknown. Here we show that dynein and dynactin exist in discrete cytosolic and membrane-bound states in the filamentous fungus Neur...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1093/embo-reports/kve202
更新日期:2001-10-01 00:00:00
abstract::Like many transcription regulators, histone methyltransferases G9a and G9a-like protein (GLP) can act gene-specifically as coregulators, but mechanisms controlling this specificity are mostly unknown. We show that adjacent post-translational methylation and phosphorylation regulate binding of G9a and GLP to heterochro...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201744060
更新日期:2017-08-01 00:00:00
abstract::During the past years, yeasts have been successfully established as models to study the mechanisms of apoptotic regulation. We recently showed that mutations in the LSM4 gene, which is involved in messenger RNA decapping, lead to increased mRNA stability and apoptosis in yeast. Here, we show that mitochondrial functio...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400514
更新日期:2005-11-01 00:00:00