Abstract:
:Individuals affected by the autosomal recessive disorder Werner's syndrome (WS) develop many of the symptoms characteristic of premature ageing. Primary fibroblasts cultured from WS patients exhibit karyotypic abnormalities and a reduced replicative life span. The WRN gene encodes a 3'-5' DNA helicase, and is a member of the RecQ family, which also includes the product of the Bloom's syndrome gene (BLM). In this work, we show that WRN promotes the ATP-dependent translocation of Holliday junctions, an activity that is also exhibited by BLM. In cells arrested in S-phase with hydroxyurea, WRN localizes to discrete nuclear foci that coincide with those formed by the single-stranded DNA binding protein replication protein A. These results are consistent with a model in which WRN prevents aberrant recombination events at sites of stalled replication forks by dissociating recombination intermediates.
journal_name
EMBO Repjournal_title
EMBO reportsauthors
Constantinou A,Tarsounas M,Karow JK,Brosh RM,Bohr VA,Hickson ID,West SCdoi
10.1093/embo-reports/kvd004keywords:
subject
Has Abstractpub_date
2000-07-01 00:00:00pages
80-4issue
1eissn
1469-221Xissn
1469-3178pii
embor632journal_volume
1pub_type
杂志文章相关文献
EMBO REPORTS文献大全abstract::The highly homologous Cl(-) channels CLC-Ka and CLC-Kb are important for water and salt conservation in the kidney and for the production of endolymph in the inner ear. Mutations in CLC-Kb lead to Bartter's syndrome and mutations in the small CLC-K subunit barttin lead to Bartter's syndrome and deafness. Here we show ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400169
更新日期:2004-06-01 00:00:00
abstract::Ubiquitylation is an essential post-translational modification that regulates numerous cellular processes, most notably protein degradation. Ubiquitin can itself be phosphorylated at nearly every serine, threonine, and tyrosine residue. However, the effect of this modification on ubiquitin function is largely unknown....
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201540298
更新日期:2015-09-01 00:00:00
abstract::Synaptic vesicles fuse with the plasma membrane in response to Ca(2+) influx, thereby releasing neurotransmitters into the synaptic cleft. The protein machinery that mediates this process, consisting of soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) and regulatory proteins, is well kno...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1002/embr.201337807
更新日期:2014-03-01 00:00:00
abstract::The active and inactive X chromosomes have distinct epigenetic marks in somatic nuclei, which undergo reprogramming after transplantation into oocytes. We show that, despite the disappearance of Xist RNA coating in 30 min, the epigenetic memory of the inactive X persists with the precocious appearance of histone H3 tr...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400461
更新日期:2005-08-01 00:00:00
abstract::The components and pathways that regulate and execute developmental cell death programmes in plants remain largely unknown. We have found that the PROMOTION OF CELL SURVIVAL 1 (PCS1) gene in Arabidopsis, which encodes an aspartic protease, has an important role in determining the fate of cells in embryonic development...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400357
更新日期:2005-03-01 00:00:00
abstract::The EMBO/EMBL symposium 'Human Variation: Cause and Consequence' highlighted advances in understanding the molecular basis of human genetic variation and its myriad implications for biology, human origins and disease. As high-throughput sequencing allows us to define genetic variation and its functional consequences a...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/embor.2010.126
更新日期:2010-09-01 00:00:00
abstract::Polyploidization frequently precedes tumorigenesis but also occurs during normal development in several tissues. Hepatocyte ploidy is controlled by the PIDDosome during development and regeneration. This multi-protein complex is activated by supernumerary centrosomes to induce p53 and restrict proliferation of polyplo...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.202050893
更新日期:2020-11-23 00:00:00
abstract::The COVID-19 crisis has further highlighted the challenges for open science and data sharing in biomedical research and the need for more traceability and transparency. ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.202051773
更新日期:2020-11-05 00:00:00
abstract::Toll-like receptor 2 (TLR2) has been shown to recognize several classes of pathogen-associated molecular patterns including peptidoglycan (PG). However, studies linking PG with TLR2 recognition have relied mainly on the use of commercial Staphylococcus aureus PG and have not addressed TLR2 recognition of other PG type...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400248
更新日期:2004-10-01 00:00:00
abstract::Sirtuins (SIRTs) are a class of lysine deacylases that regulate cellular metabolism and energy homeostasis. Although sirtuins have been proposed to function in nutrient sensing and signaling, the underlying mechanism remains elusive. SIRT7, a histone H3K18-specific deacetylase, epigenetically controls mitochondria bio...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201846377
更新日期:2018-12-01 00:00:00
abstract::Codon bias has been implicated as one of the major factors contributing to mRNA stability in several model organisms. However, the molecular mechanisms of codon bias on mRNA stability remain unclear in humans. Here, we show that human cells possess a mechanism to modulate RNA stability through a unique codon bias. Bio...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201948220
更新日期:2019-11-05 00:00:00
abstract::The ubiquitin-binding zinc finger (UBZ) domain of human DNA Y-family polymerase (pol) eta is important in the recruitment of the polymerase to the stalled replication machinery in translesion synthesis. Here, we report the solution structure of the pol eta UBZ domain and its interaction with ubiquitin. We show that th...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400901
更新日期:2007-03-01 00:00:00
abstract::The cytoplasm is the main place for protein translation from where nascent proteins are transported to their working areas, including the inside, outside, and membrane of the cell. The majority of newly synthesized membrane proteins is co-translationally inserted into the membrane by the evolutionary conserved Sec tra...
journal_title:EMBO reports
pub_type: 评论,杂志文章
doi:10.15252/embr.201949034
更新日期:2019-10-04 00:00:00
abstract::Extracellular vesicles (EVs) have been shown to carry microbial components and function in the host defense against infections. In this study, we demonstrate that Mycobacterium tuberculosis (M.tb) RNA is delivered into macrophage-derived EVs through an M.tb SecA2-dependent pathway and that EVs released from M.tb-infec...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201846613
更新日期:2019-03-01 00:00:00
abstract::Activation of the elongation factor 2 kinase (eEF2K) leads to the phosphorylation and inhibition of the elongation factor eEF2, reducing mRNA translation rates. Emerging evidence indicates that the regulation of factors involved in protein synthesis may be critical for controlling diverse biological processes includin...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201642194
更新日期:2016-10-01 00:00:00
abstract::The role of long noncoding RNAs (lncRNAs) in viral infection is poorly studied. We have identified hepatitis C virus (HCV)-Stimulated lncRNAs (CSRs) by transcriptome analysis. Interestingly, two of these CSRs (PVT1 and UCA1) play relevant roles in tumorigenesis, providing a novel link between HCV infection and develop...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201541763
更新日期:2016-07-01 00:00:00
abstract::The Lgr5 receptor is a marker of intestinal stem cells (ISCs) that regulates Wnt/b-catenin signaling. In this study, phenotype analysis of knockin/knockout Lgr5-eGFP-IRES-Cre and Lgr5-DTReGFP embryos reveals that Lgr5 deficiency during Wnt-mediated cytodifferentiation results in amplification of ISCs and early differe...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201949224
更新日期:2020-07-03 00:00:00
abstract::Estrogen receptors (ERs) orchestrate both transcriptional and non-genomic functions in response to estrogens, xenoestrogens and signals emanating from growth factor signalling pathways. The pleiotropic and tissue-specific effects of estrogens are likely to be mediated by the differential expression of distinct estroge...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.1093/embo-reports/kve185
更新日期:2001-09-01 00:00:00
abstract::RNA silencing is a complex of mechanisms that regulate gene expression through small RNA molecules. The microRNA (miRNA) pathway is the most common of these in mammals. Genome-encoded miRNAs suppress translation in a sequence-specific manner and facilitate shifts in gene expression during developmental transitions. He...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.1038/embor.2010.102
更新日期:2010-08-01 00:00:00
abstract::Streptococcus pneumoniae causes more than one million deaths every year, mostly of young children in developing countries, due to pneumonia, bacteremia and meningitis. The emergence and dissemination of drug-resistant pneumococcal strains, coupled to changing patterns of virulence and the inadequacy of available vacci...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.1093/embo-reports/kvf152
更新日期:2002-08-01 00:00:00
abstract::The Wnt signaling pathway can be grouped into two classes, the β-catenin-dependent and β-catenin-independent pathways. Wnt5a signaling through a β-catenin-independent pathway promotes microtubule (MT) remodeling during cell-substrate adhesion, cell migration, and planar cell polarity formation. Although Wnt5a signalin...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201745471
更新日期:2018-07-01 00:00:00
abstract::The developmental and oncogenic roles of MYC proteins are well established, but the transcriptional targets mediating their functions remain elusive. Using small interfering RNA-mediated knockdown in breast and cervix carcinoma cell lines, which overexpress c-MYC, we show that c-MYC independently controls metabolism a...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400849
更新日期:2007-01-01 00:00:00
abstract::Global climate change and the increasing human population require crop varieties with higher yield and draught resistance. But meeting both goals is not an easy task for breeders and plant science. ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.202051598
更新日期:2020-11-29 00:00:00
abstract::While the genome sequence and gene content are available for an increasing number of organisms, eukaryotic selenoproteins remain poorly characterized. The dual role of the UGA codon confounds the identification of novel selenoprotein genes. Here, we describe a comparative genomics approach that relies on the genome-wi...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.1038/sj.embor.7400036
更新日期:2004-01-01 00:00:00
abstract::IQGAP scaffold proteins are evolutionarily conserved in eukaryotes and facilitate the formation of complexes that regulate cytoskeletal dynamics, intracellular signaling, and intercellular interactions. Fungal and mammalian IQGAPs are implicated in cytokinesis. IQGAP1, IQGAP2, and IQGAP3 have diverse roles in vertebra...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.15252/embr.201439834
更新日期:2015-04-01 00:00:00
abstract::Despite its limited resources, Portugal has gained a prominent position in research on malaria. Several historical and personal factors have contributed to this achievement. ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.202051692
更新日期:2021-01-10 00:00:00
abstract::Reading the academic literature is an essential part of a scientist's job. David Smith argues that reading fiction can be even more important for creativity and productivity than papers and reviews. ...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.202052206
更新日期:2021-01-04 00:00:00
abstract::The systematic recording and management of experimental data in academic life science research remains an open problem. EPFL engaged in a program of ELN/LIMS deployment 6 years ago, encountering a host of fundamental questions at the institutional level and in each single laboratory. Here, based on our experience we a...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201949862
更新日期:2020-03-04 00:00:00
abstract::Protein kinase D (PKD) mediates the actions of stimuli that promote diacylglycerol (DAG) biogenesis. By phosphorylating effectors that regulate transcription, fission and polarized transport of Golgi vesicles, as well as cell migration and survival after oxidative stress, PKDs substantially expand the range of physiol...
journal_title:EMBO reports
pub_type: 杂志文章,评审
doi:10.1038/embor.2011.139
更新日期:2011-07-08 00:00:00
abstract::The Nedd4 family E3 ligases are key regulators of cell growth and proliferation and are often misregulated in human cancers and other diseases. The ligase activities of Nedd4 E3s are tightly controlled via auto-inhibition. However, the molecular mechanism underlying Nedd4 E3 auto-inhibition and activation is poorly un...
journal_title:EMBO reports
pub_type: 杂志文章
doi:10.15252/embr.201744454
更新日期:2017-09-01 00:00:00