Abstract:
:Initiation of DNA replication from the Escherichia coli chromosomal origin is highly regulated, assuring that replication occurs precisely once per cell cycle. Three mechanisms for regulation of replication initiation have been proposed: titration of free DnaA initiator protein by the datA locus, sequestration of newly replicated origins by SeqA protein and regulatory inactivation of DnaA (RIDA), in which active ATP-DnaA is converted to the inactive ADP-bound form. DNA microarray analyses showed that the level of initiation in rapidly growing cells that lack datA was indistinguishable from that in wild-type cells, and that the absence of SeqA protein caused only a modest increase in initiation, in agreement with flow-cytometry data. In contrast, cells lacking Hda overinitiated replication twofold, implicating RIDA as the predominant mechanism preventing extra initiation events in a cell cycle.
journal_name
EMBO Repjournal_title
EMBO reportsauthors
Camara JE,Breier AM,Brendler T,Austin S,Cozzarelli NR,Crooke Edoi
10.1038/sj.embor.7400467keywords:
subject
Has Abstractpub_date
2005-08-01 00:00:00pages
736-41issue
8eissn
1469-221Xissn
1469-3178pii
7400467journal_volume
6pub_type
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