Optimizing an ultrasound contrast agent's stability using in vitro attenuation measurements.

Abstract:

RATIONALE AND OBJECTIVES:To evaluate the changes in the microbubble population of a currently available ultrasound contrast agent (USCA) through attenuation measurements to optimize its clinical use. MATERIALS AND METHODS:The microbubble population from a galactose-based USCA (Levovist, Schering AG, Germany) was characterized in vitro using attenuation measurements. The effect of dose (0.1, 0.5, and 1 mL), concentrations (200, 300, and 400 mg mL(-1)) and time since reconstitution (2, 12, 22, and 32 minutes) was evaluated using two broadband pulses at different peak negative pressures (0.39 and 0.49 MPa) for a total of 72 injections. RESULTS:Two minutes after reconstitution, a linear relationship was found between attenuation measurements and the amount of USCA (slope 0.92 dB x mg x cm(-1) ml(-1), R = 0.86). For a given dose and concentration, the microbubble stability was significantly reduced with the increase of the time since reconstitution, particularly for the lower concentrations. CONCLUSIONS:The persistence and contrast effect of Levovist can be improved by using recommended minimum time since reconstitution and maximum concentration.

journal_name

Invest Radiol

journal_title

Investigative radiology

authors

Correas JM,Kurtisovski E,Bridal SL,Amararene A,Hélénon O,Berger G

doi

10.1097/00004424-200212000-00006

keywords:

subject

Has Abstract

pub_date

2002-12-01 00:00:00

pages

672-9

issue

12

eissn

0020-9996

issn

1536-0210

journal_volume

37

pub_type

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