Abstract:
:The stimulatory effect of lipopolysaccharide (LPS) on human macrophages was found to be neutralized by the detergent octylglucoside (OG). Both macrophage stimulation and reactivity in a limulus amebocyte lysate test were suppressed by suspension of LPS in OG at concentrations between 0.25 and 2.5 mM, whereas other stimulatory lipopeptides and lipid containing stimulants were unaffected by OG. LPS at concentrations causing maximal stimulation of macrophages could be completely neutralized by non-toxic concentrations of OG. In addition, it was found that the neutralization in complex mixtures of macromolecules, such as bacterial cell lysate, was specific for LPS and that the stimulatory activity of the other substances in the mixture was not affected by the OG.
journal_name
Med Microbiol Immunoljournal_title
Medical microbiology and immunologyauthors
Henrich B,Guadarrama R,Zähringer U,MacKenzie CRdoi
10.1007/s430-001-8019-2keywords:
subject
Has Abstractpub_date
2001-04-01 00:00:00pages
133-6issue
3eissn
0300-8584issn
1432-1831journal_volume
189pub_type
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