当前位置： SCI文献检索 > MEDICAL MICROBIOLOGY AND IMMUNOLOGY期刊下所有文献 > In vitro binding of Campylobacter jejuni surface proteins to murine small intestinal cell membranes.

In vitro binding of Campylobacter jejuni surface proteins to murine small intestinal cell membranes.

Abstract：

:Surface proteins of nine Campylobacter jejuni strains belonging to three different serovia were extracted with lysozyme/ethylenediamine-tetraacetic acid. The preparations bound to isolated murine small intestinal cells and to a membrane fraction (MF) of isolated brush borders obtained by detergent treatment with Triton X-100 and Nonidet P40. Binding was demonstrated by an enzyme-linked immunosorbent assay procedure. Using lipopolysaccharide (LPS)- and flagella-specific antisera the contribution of flagella and LPS, present in the protein preparations, to the total binding to MF was investigated. Only up to approximately 10% of the total binding of each strain was found to be mediated by LPS, 10%-33% of binding was flagella dependent. The preparations of four strains (serovar HS2) bound in a trypsin-sensitive manner (45%-85% reduction), while the others (serovaria HS1 and HS13) were hardly influenced by trypsin treatment. Binding to MF was not impaired by preincubation of the bacterial surface protein preparations with several sugars and lectins.

journal_name

Med Microbiol Immunol

journal_title

Medical microbiology and immunology

authors

Moser I,Hellmann E

doi

10.1007/BF00202555

subject

Has Abstract

pub_date

1989-01-01 00:00:00

pages

217-28

issue

4

eissn

0300-8584

issn

1432-1831

journal_volume

178

pub_type

杂志文章

相关文献

MEDICAL MICROBIOLOGY AND IMMUNOLOGY文献大全
  • Interaction between Clostridium difficile and polymorphonuclear leucocytes from the elderly and post-operative cancer patients: phagocytosis and bactericidal function.

    abstract：:The ability of polymorphonuclear leucocytes (PMNs) from elderly healthy subjects and post-operative cancer patients to ingest and kill Clostridium difficile was studied. The percentage of phagocytosis in clinically healthy subjects aged 69-82 years ranged from 20.2 +/- 3.2 (mean +/- SD) to 34.1 +/- 4.2, depending on t...

    journal_title：Medical microbiology and immunology

    pub_type： 杂志文章

    doi：10.1007/BF02123569

    authors： Bassaris HP,Lianou PE,Legakis NJ,Papavassiliou JT

    更新日期：1984-01-01 00:00:00

  • Positive camp-phenomenon elicited by coagulase-negative staphylococci.

    abstract：:Fifty out of sixty-five isolates of coagulase-negative staphylococci displayed a positive CAMP-reaction due to synergism of an extracellular toxin with S. aureus beta-toxin on sheep erythrocytes. Of the CAMP-positive isolates, 40 were identified as S. epidermidis, 8 as S. hemolyticus, and 2 each as S. hominis and S. s...

    journal_title：Medical microbiology and immunology

    pub_type： 杂志文章

    doi：10.1007/BF02124812

    authors： Bhakdi S

    更新日期：1985-01-01 00:00:00

  • Gentamicin as a bactericidal antibiotic in tissue culture.

    abstract：:The effects of gentamicin on cellular physiology were studied in a total of 9 mammalian cell lines, using the following parameters: cell morphology and viability (cytotoxicity), proliferation, culture medium acidification, lactic acid production, lactate dehydrogenase release, virus susceptibility, and effects on kary...

    journal_title：Medical microbiology and immunology

    pub_type： 杂志文章

    doi：10.1007/BF02120767

    authors： Fischer AB

    更新日期：1975-01-01 00:00:00

  • Enhancement of the pathogenicity of Staphylococcus aureus strain Newman by a small noncoding RNA SprX1.

    abstract：:The pathogenesis of Staphylococcus aureus from local infection to systemic dissemination involves a range of virulence factors including structural and secreted products. Among various control mechanisms, small noncoding RNAs are involved in the regulation of multiple pathogenicity factors in S. aureus. The sRNA SprX ...

    journal_title：Medical microbiology and immunology

    pub_type： 杂志文章

    doi：10.1007/s00430-016-0467-9

    authors： Kathirvel M,Buchad H,Nair M

    更新日期：2016-12-01 00:00:00

  • An immunodominant 30-kDa antigen of a candidate anti-leprosy vaccine, Mycobacterium w, shares T and B cell determinants with M. leprae and M. tuberculosis.

    abstract：:Earlier we reported that vaccination of leprosy patients with Mycobacterium w induces an immune response directed predominantly against low molecular weight antigens. One of these antigens, with a molecular mass of 30-kDa, was recognized by a majority of the vaccinated subjects as well as the tuberculoid leprosy patie...

    journal_title：Medical microbiology and immunology

    pub_type： 杂志文章

    doi：10.1007/BF00579623

    authors： Yadava A,Mukherjee R

    更新日期：1993-11-01 00:00:00

  • Bordetella Pertussis Toxin does not induce the release of pro-inflammatory cytokines in human whole blood.

    abstract：:Pertussis Toxin (PTx) is one of the most important virulence factors of Bordetella pertussis, the cause of whooping cough. Therefore, the inactivated toxin is an obligatory constituent of acellular pertussis vaccines. It is described in the literature that both native PTx and recombinant Pertussis Toxin (PTg) activate...

    journal_title：Medical microbiology and immunology

    pub_type： 杂志文章

    doi：10.1007/s00430-012-0238-1

    authors： Bache C,Spreitzer I,Becker B,Loeschner B,Rosskopf U,Hanschmann KM,Schwanig M,Schneider CK,Lieb B,Montag T

    更新日期：2012-08-01 00:00:00

  • The prevalence of TT virus (TTV) infection and its relationship to hepatitis in children.

    abstract：:TT virus (TTV) is a newly discovered virus from a patient with post-transfusion hepatitis. We investigated the frequency and pathogenesis of TTV infection in children. A semi-nested PCR assay was used to amplify TTV-DNA in serum samples from 254 ambulatory children without liver disease, 20 with hepatitis of unknown e...

    journal_title：Medical microbiology and immunology

    pub_type： 杂志文章

    doi：10.1007/s004300050109

    authors： Iriyama M,Kimura H,Nishikawa K,Yoshioka K,Wakita T,Nishimura N,Shibata M,Ozaki T,Morishima T

    更新日期：1999-11-01 00:00:00

  • Nonspecific inhibitors of coronavirus OC43 haemagglutination in human sera.

    abstract：:Antibodies against the human coronavirus OC43 in human sera were measured by haemagglutination inhibition (HI), complement fixation (CF), and radial diffusion-haemolysis in gel (HIG) techniques. The apparent HI titres in a fraction of sera with no antibodies detectable by the two other methods were found to be reduced...

    journal_title：Medical microbiology and immunology

    pub_type： 杂志文章

    doi：10.1007/BF02121147

    authors： Hovi T

    更新日期：1978-11-17 00:00:00

  • T-cell immunity against cytomegalovirus in HIV infection and aging: relationships with inflammation, immune activation, and frailty.

    abstract：:Both aging and treated human immunodeficiency virus (HIV) infection are characterized by low-level chronic inflammation and immune activation which contribute to the development of age-related diseases, frailty, and early mortality. Chronic cytomegalovirus (CMV) infection is highly prevalent in older adults and HIV-in...

    journal_title：Medical microbiology and immunology

    pub_type： 杂志文章,评审

    doi：10.1007/s00430-019-00591-z

    authors： Tavenier J,Margolick JB,Leng SX

    更新日期：2019-08-01 00:00:00

  • Highly individual patterns of virus-immune IgG effector responses in humans.

    abstract：:IgG responses are fundamental to adaptive immunity and document immunological memory of previous pathogen encounter. While specific antigen recognition is mediated by the variable F(ab')2 domain of IgG, various effector functions become activated via the constant Fcγ part bridging IgG-opsonized targets to FcγR-express...

    journal_title：Medical microbiology and immunology

    pub_type： 杂志文章

    doi：10.1007/s00430-016-0457-y

    authors： Corrales-Aguilar E,Trilling M,Reinhard H,Falcone V,Zimmermann A,Adams O,Santibanez S,Hengel H

    更新日期：2016-10-01 00:00:00

  • Identification of RNA-binding regions on the P and V proteins of human parainfluenza virus type 2.

    abstract：:We have shown that the P and V proteins of human parainfluenza virus type 2 (hPIV-2) bind to genomic RNA by using Northwestern blot analysis. To identify the RNA-binding regions on the P and V proteins, we used a set of deletion mutants produced in Escherichia coli. One region required for the RNA-binding was found in...

    journal_title：Medical microbiology and immunology

    pub_type： 杂志文章

    doi：10.1007/s00430-005-0244-7

    authors： Nishio M,Tsurudome M,Ito M,Ito Y

    更新日期：2006-03-01 00:00:00

  • Clearance of Chlamydia trachomatis-induced polyserositis in SCID mice requires both CD4+ and CD8+ cells.

    abstract：:To characterize the role of specific lymphocyte subsets in Chlamydia trachomatis infection, we established a murine model using the mouse pneumonitis agent (MoPn) of C. trachomatis and C.B-17 scid/scid (SCID) mice which lack functional B and T cells. After intraperitoneal inoculation with the bacteria, SCID mice devel...

    journal_title：Medical microbiology and immunology

    pub_type： 杂志文章

    doi：10.1007/s004300050076

    authors： Thoma-Uszynski S,Simnacher U,Marre R,Essig A

    更新日期：1998-10-01 00:00:00

  • Isolation of two antigens from the culture filtrates of Mycobacterium tuberculosis and their applications in the laboratory diagnosis of the tuberculous meningitis.

    abstract：:Two antigens were isolated from the culture filtrates of H37Ra Mycobacterium tuberculosis by immunoabsorbent affinity chromatography, M. tuberculosis antigen 5 and immunoabsorbent affinity column-purified antigen (IAP). The potential application of these two mycobacterial antigens in the laboratory diagnosis of tuberc...

    journal_title：Medical microbiology and immunology

    pub_type： 杂志文章

    doi：10.1007/BF00193851

    authors： Krishnan VV,Mathai A

    更新日期：1991-01-01 00:00:00

  • Herpes simplex and varicella-zoster virus infections during pregnancy: current concepts of prevention, diagnosis and therapy. Part 2: Varicella-zoster virus infections.

    abstract：:Varicella during pregnancy can be associated with severe illnesses for both the mother and her neonate. Varicella pneumonia must be regarded as a medical emergency, since pregnant women are at risk of life-threatening ventilatory compromise and death. After maternal chickenpox in the first and second trimesters, conge...

    journal_title：Medical microbiology and immunology

    pub_type： 杂志文章,评审

    doi：10.1007/s00430-006-0032-z

    authors： Sauerbrei A,Wutzler P

    更新日期：2007-06-01 00:00:00

  • [Structure and function of virus-induced antigens in cultured cells infected with Merek's disease and turkey herpes viruses. II. Isolation of intracellular antigens from infected cells (author's transl)].

    abstract：:Virus-induced antigens were isolated from MDV and HVT-infected cells by salt extraction with 3M KCl and purified by Con.A chromatography and isoelectric focusing. Electrophoretic analysis of 35S-methionine, 3H-fucose and 32P-orthophosphate labeled antigens revealed 7 different polypeptides, two of them containing labe...

    journal_title：Medical microbiology and immunology

    pub_type： 杂志文章

    doi：10.1007/BF02126676

    authors： Kaaden OR

    更新日期：1977-10-07 00:00:00

  • Characterization of Borrelia burgdorferi sensu lato strains isolated from Ixodes ricinus in Mecklenburg-Vorpommern, Germany.

    abstract：:Epidemiological studies in Mecklenburg-Vorpommern have shown a high prevalence of Borrelia burgdorferi-infected ticks. A total of 17 B. burgdorferi sensu lato strains were isolated from ticks and investigated by Western blots (immunoblot) with eight monoclonal anti-bodies against different epitopes of the outer surfac...

    journal_title：Medical microbiology and immunology

    pub_type： 杂志文章

    doi：

    authors： Lottmann H,Wilske B,Herrmann H

    更新日期：1996-02-01 00:00:00

  • Performance of two MALDI-TOF MS systems for the identification of yeasts isolated from bloodstream infections and cerebrospinal fluids using a time-saving direct transfer protocol.

    abstract：:The rapid and correct identification of pathogens is of paramount importance for the treatment of patients with invasive infections. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) can speed up the identification of bacteria and fungi and has quickly been embraced by medical...

    journal_title：Medical microbiology and immunology

    pub_type： 杂志文章

    doi：10.1007/s00430-013-0319-9

    authors： Hamprecht A,Christ S,Oestreicher T,Plum G,Kempf VA,Göttig S

    更新日期：2014-04-01 00:00:00

  • Effects of genetic resistance against Herpes simplex virus in vaginally infected mice.

    abstract：:In order to take the conditions of natural Herpes simplex virus (HSV) infection into consideration, the genetic resistance of C57-Bl mice, which was established by intraperitoneal HSV-1 infection [Lopez, 1975], was investigated in vaginally infected mice. The course of infection in the mucous membranes did not differ ...

    journal_title：Medical microbiology and immunology

    pub_type： 杂志文章

    doi：10.1007/BF02123624

    authors： Schneweis KE,Olbrich M,Saftig V,Scholz R

    更新日期：1982-01-01 00:00:00

  • Re-evaluation of the Auxotab (Inolex) Enteric 1 System for identification of enterobacteriaceae.

    abstract：:The improved Auxotab Enteric I System was re-evaluated and found to correlate 98% with conventional methods for the identification of Enterobacteriaceae. Possible Reasons for previous poor results with this system are discussed. ...

    journal_title：Medical microbiology and immunology

    pub_type： 杂志文章

    doi：10.1007/BF02121009

    authors： Braune LM,Kocka FE

    更新日期：1975-07-02 00:00:00

  • Kaposi's sarcoma-associated herpesvirus seroprevalence in selected german patients: evaluation by different test systems.

    abstract：:A total of 603 serum samples obtained from 12 different patient and control groups, including potentially cross-reactive sera, were tested for the presence of antibodies against Kaposi's sarcoma-associated herpesvirus (KSHV, HHV-8). The assays used were an inhouse immunofluorescence test (IFT) employing latent KSHV an...

    journal_title：Medical microbiology and immunology

    pub_type： 杂志文章

    doi：10.1007/s00430-001-0098-6

    authors： Preiser W,Szép NI,Lang D,Doerr HW,Rabenau HF

    更新日期：2001-12-01 00:00:00

  • Enterotoxigenic and enteropathogenic Escherichia coli in Galicia (north-west Spain).

    abstract：:We have studied the incidence of enterotoxigenic and enteropathogenic Escherichia coli strains associated with infant diarrhoeal disease in Galicia (North-west Spain). During a period of 9 months we isolated heat-labile enterotoxin-positive strains in 2.1% of children with diarrhoea examined, whereas the production of...

    journal_title：Medical microbiology and immunology

    pub_type： 杂志文章

    doi：10.1007/BF02123802

    authors： Blanco J,González EA,Bernárdez I,Varela BR

    更新日期：1983-01-01 00:00:00

  • Influence of lactoferrin on the entry process of Escherichia coli HB101 (pRI203) in HeLa cells.

    abstract：:Lactoferrin (Lf) is an iron-binding protein which plays an important role in the host defense systems of different mucosal surfaces including the intestinal mucosa. In the present research the role of apo-Lf and iron-saturated Lf in the invasion process of enteroinvasive bacteria, grown in iron stress or excess, was i...

    journal_title：Medical microbiology and immunology

    pub_type： 杂志文章

    doi：10.1007/BF00195948

    authors： Longhi C,Conte MP,Seganti L,Polidoro M,Alfsen A,Valenti P

    更新日期：1993-03-01 00:00:00

  • Characterization and immunogenicity of a candidate subunit vaccine against varicella-zoster virus.

    abstract：:This study describes the properties of an inactivated subunit antigen preparation from varicella-zoster virus (VZV)-infected MRC-5 cells by treatment with detergent and formaldehyde, ultracentrifugation over sucrose and acetone precipitation. The method preserved the antigenicity of VZV proteins and several VZV-specif...

    journal_title：Medical microbiology and immunology

    pub_type： 临床试验,杂志文章

    doi：10.1007/BF00277161

    authors： Davies J,Hallworth JA,McLeish P,Randall S,Martin BA,Buchan A,Skinner GR

    更新日期：1994-05-01 00:00:00

  • Isolation of a 33-kDa protein antigen from delipidified Mycobacterium tuberculosis H37Rv.

    abstract：:A 33-kDa protein (TB33) was isolated from a delipidated cell sonicate (CS) of Mycobacterium tuberculosis H37Rv (grown in Middlebrook 7H9 broth supplemented with glucose) using immobilized metal affinity chromatography (IMAC) on a nickel-nitrilotriacetic acid (Ni-NTA) column. TB33 could not be isolated from the culture...

    journal_title：Medical microbiology and immunology

    pub_type： 杂志文章

    doi：10.1007/s004300050025

    authors： Deshpande RG,Khan MB,Bhat DA,Navalkar RG

    更新日期：1996-11-01 00:00:00

  • The Leishmania genome project: new insights into gene organization and function.

    abstract：:The sequencing of Leishmania major Friedlin chromosome 1 (Chr1), Chr3, and Chr4 has been completed. and several other chromosomes are well underway. The complete genome sequence should be available by 2003. Over 1,000 full-length new genes have been identified, with the majority (approximately 75%) having unknown func...

    journal_title：Medical microbiology and immunology

    pub_type： 杂志文章

    doi：10.1007/s004300100070

    authors： Myler PJ,Beverley SM,Cruz AK,Dobson DE,Ivens AC,McDonagh PD,Madhubala R,Martinez-Calvillo S,Ruiz JC,Saxena A,Sisk E,Sunkin SM,Worthey E,Yan S,Stuart KD

    更新日期：2001-11-01 00:00:00

  • Recognition by peptide mapping of three different structural groups of outer membrane protein YOP-1 of Yersinia enterocolitica and Yersinia pseudotuberculosis.

    abstract：:The structural relation of YOP-1 of "european" and "american" Yersinia enterocolitica serotypes O:3, O:9, O:5,27, and O:8 and O:20, respectively, and Y. pseudotuberculosis serotypes I, II, and III was compared by sodium dodecyl sulfate polyacrylamide gel electrophoresis and peptide mapping using Staphylococcus aureus ...

    journal_title：Medical microbiology and immunology

    pub_type： 杂志文章

    doi：10.1007/BF00215249

    authors： Mack D,Pulz M,Heesemann J

    更新日期：1991-01-01 00:00:00

  • Binding of the V proteins to the nucleocapsid proteins of human parainfluenza type 2 virus.

    abstract：:Interaction of the nucleocapsid (NP) and V proteins of human parainfluenza type 2 virus (HPIV-2) was investigated using a transient expression system. When the NP proteins were co-expressed with the V proteins, some of the NP proteins were translocated into the nuclei. These findings suggest that the NP protein intera...

    journal_title：Medical microbiology and immunology

    pub_type： 杂志文章

    doi：10.1007/s004300050019

    authors： Watanabe N,Kawano M,Tsurudome M,Nishio M,Ito M,Ohgimoto S,Suga S,Komada H,Ito Y

    更新日期：1996-09-01 00:00:00

  • Wolbachia endobacteria depletion by doxycycline as antifilarial therapy has macrofilaricidal activity in onchocerciasis: a randomized placebo-controlled study.

    abstract：:In a randomized, placebo-controlled trial in Ghana, 67 onchocerciasis patients received 200-mg/day doxycycline for 4-6 weeks, followed by ivermectin (IVM) after 6 months. After 6-27 months, efficacy was evaluated by onchocercoma histology, PCR and microfilariae determination. Administration of doxycycline resulted in ...

    journal_title：Medical microbiology and immunology

    pub_type： 杂志文章,随机对照试验

    doi：10.1007/s00430-007-0062-1

    authors： Hoerauf A,Specht S,Büttner M,Pfarr K,Mand S,Fimmers R,Marfo-Debrekyei Y,Konadu P,Debrah AY,Bandi C,Brattig N,Albers A,Larbi J,Batsa L,Taylor MJ,Adjei O,Büttner DW

    更新日期：2008-09-01 00:00:00

  • The influence of the intestinal microflora on the relative thymus weight.

    abstract：:Oral treatment of mice with a combination of non-absorbable broad spectrum antibiotics at a dose level high enough to sterilize the digestive tract within five days of treatment, has been found to have a significant negative effect on the relative weight of the thymus. From the age of 12 weeks onwards, the mean relati...

    journal_title：Medical microbiology and immunology

    pub_type： 杂志文章

    doi：10.1007/BF02123870

    authors： van der Waaij D

    更新日期：1986-01-01 00:00:00

  • Differentiation of Borrelia burgdorferi sensu lato strains using class I lysyl-tRNA synthetase-encoding genes.

    abstract：:The essential protein lysyl-tRNA synthetase (LysRS) exists in two unrelated forms, a class I and a class II-type aminoacyl-tRNA synthetase. Comparative genome sequence analysis revealed that Borrelia burgdorferi sensu lato, the etiological agent of Lyme disease, contains a class I-type LysRS, whereas its tick and mamm...

    journal_title：Medical microbiology and immunology

    pub_type： 杂志文章

    doi：10.1007/s00430-002-0149-7

    authors： Mejlhede N,Monthán A,Theisen M,Ibba M

    更新日期：2003-05-01 00:00:00