Abstract:
:Two antigens were isolated from the culture filtrates of H37Ra Mycobacterium tuberculosis by immunoabsorbent affinity chromatography, M. tuberculosis antigen 5 and immunoabsorbent affinity column-purified antigen (IAP). The potential application of these two mycobacterial antigens in the laboratory diagnosis of tuberculous meningitis was evaluated by indirect enzyme-linked immunoabsorbent assay in cerebrospinal fluid specimens. IAP antigen was more sensitive than antigen 5, although antigen 5 was more specific than IAP antigen in detecting tuberculous aetiology. Technical aspects of immunoabsorbent affinity chromatography have been highlighted in this study.
journal_name
Med Microbiol Immunoljournal_title
Medical microbiology and immunologyauthors
Krishnan VV,Mathai Adoi
10.1007/BF00193851subject
Has Abstractpub_date
1991-01-01 00:00:00pages
101-7issue
2eissn
0300-8584issn
1432-1831journal_volume
180pub_type
杂志文章abstract::Interactions of virulent Leptospira interrogans with murine monocyte-macrophage-like J774A.1 cells and Vero (African green monkey kidney fibroblasts) cells from attachment to internalization were investigated by a series of morphological analysis. Fontana silver staining revealed that only the pathogenic leptospires w...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-007-0047-0
更新日期:2007-12-01 00:00:00
abstract::Escherichia coli isolates of serotype O6:K5 are the most common causative agents of cystitis and pyelonephritis in adults. To answer the question, as to whether strains of this particular serotype represent one special clonal group, out of a collection of 34 serotype O6:K5 isolates [Zingler et al. (1990) Zentralbl. Ba...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF00195947
更新日期:1993-03-01 00:00:00
abstract::The presence of Borna disease virus (BDV)-specific RNA was traced by the reverse transcriptase-polymerase chain reaction in conjunctival fluid, nasal secretions and saliva of horses which were seropositive but did not have any history of clinical Borna disease. Positive reactions encompassed sequences encoding the p24...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF00191945
更新日期:1993-12-01 00:00:00
abstract::Immunodominant proteins are variable in molecular and antigenic structure among different genospecies of Borrelia burgdorferi sensu lato. We have recently developed an immunoblot using five recombinant antigens: the chromosomal-encoded B. burgdorferi proteins p100, the flagellin and an internal flagellin fragment ther...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF00193630
更新日期:1994-02-01 00:00:00
abstract::Upon infection of human T-cell leukemia virus type I (HTLV-I)-carrying human T-cell lines such as MT-4, HTLV-III, a probable etiologic agent of acquired immune deficiency syndrome (AIDS) caused fast and strong cytopathic effects leading ultimately to the death of the cells. Such effects were preceded by the rapid indu...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02123869
更新日期:1986-01-01 00:00:00
abstract::To characterize the role of specific lymphocyte subsets in Chlamydia trachomatis infection, we established a murine model using the mouse pneumonitis agent (MoPn) of C. trachomatis and C.B-17 scid/scid (SCID) mice which lack functional B and T cells. After intraperitoneal inoculation with the bacteria, SCID mice devel...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s004300050076
更新日期:1998-10-01 00:00:00
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-002-0146-x
更新日期:2002-12-01 00:00:00
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章,评审
doi:10.1007/s00430-009-0121-x
更新日期:2009-11-01 00:00:00
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF00579623
更新日期:1993-11-01 00:00:00
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章,评审
doi:10.1007/s00430-014-0377-7
更新日期:2015-02-01 00:00:00
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-009-0136-3
更新日期:2010-02-01 00:00:00
abstract::The present study was designed to determine whether tumor induction by 3-methylcholanthrene (MC), a carcinogenic hydrocarbon, can be inhibited by oral administration of Lactobacillus casei strain Shirota (LC). C3H/HeN mice were divided into four groups and assigned to the following treatments: treated with MC and give...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s004300050112
更新日期:1999-12-01 00:00:00
abstract::The effects of sheep anti-murine recombinant tumor necrosis factor-alpha (TNF-alpha) on resistance to Listeria monocytogenes infection were studied in T cell-deficient nu/nu mice. The sheep anti-TNF-alpha antibody preparation was specific for TNF since it neutralized 300 U of recombinant murine TNF-alpha in vitro at a...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF00198530
更新日期:1990-01-01 00:00:00
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02121822
更新日期:1977-07-18 00:00:00
abstract::Virus adsorption and uptake of human rhinovirus 14 (HRV14) were studied with HeLa cells and baby hamster kidney (BHK) cells which were transfected with the HRV14 receptor intercellular adhesion molecule-1 (ICAM-1). Transmission electron microscopy of HeLa cells revealed that HRV14 was internalized via clathrin-coated ...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s004300050039
更新日期:1997-06-01 00:00:00
abstract::EBV-associated polypeptides induced in different Epstein-Barr Virus (EBV)-producing cell lines by the tumor promotor TPA, and from purified EBV particles derived from the same lines were radioactivity labeled and analyzed by immunoprecipitation with human VCA+MA+ sera. In virus-producing cells no significant differenc...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02122703
更新日期:1982-01-01 00:00:00
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02125494
更新日期:1978-01-01 00:00:00
abstract::In order to take the conditions of natural Herpes simplex virus (HSV) infection into consideration, the genetic resistance of C57-Bl mice, which was established by intraperitoneal HSV-1 infection [Lopez, 1975], was investigated in vaginally infected mice. The course of infection in the mucous membranes did not differ ...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02123624
更新日期:1982-01-01 00:00:00
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-012-0238-1
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02122676
更新日期:1981-01-01 00:00:00
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-012-0254-1
更新日期:2013-04-01 00:00:00
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-010-0174-x
更新日期:2011-05-01 00:00:00
abstract::IgG subclass antibodies to Pseudomonas aeruginosa outer membrane proteins (OMP) were investigated in serum from cystic fibrosis (CF) patients by immunoblotting and enzyme-linked immunosorbent assay (ELISA). Fifteen patients (eight in good and seven in poor clinical condition) have been followed for an average of 13 ye...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF00191546
更新日期:1992-01-01 00:00:00
abstract::In resource-limited or point-of-care settings, rapid diagnostic tests (RDTs), that aim to simultaneously detect HIV antibodies and p24 capsid (p24CA) antigen with high sensitivity, can pose important alternatives to screen for early infections. We evaluated the performance of the antibody and antigen components of the...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-019-00655-0
更新日期:2020-04-01 00:00:00
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-006-0028-8
更新日期:2007-06-01 00:00:00
abstract::The pathogenesis of Staphylococcus aureus from local infection to systemic dissemination involves a range of virulence factors including structural and secreted products. Among various control mechanisms, small noncoding RNAs are involved in the regulation of multiple pathogenicity factors in S. aureus. The sRNA SprX ...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-016-0467-9
更新日期:2016-12-01 00:00:00
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-011-0217-y
更新日期:2012-05-01 00:00:00
abstract::Thrombi formation was demonstrated in mesenteric microcirculation of guinea pigs inoculated with Fusobacterium necrophorum and the bacterial hemagglutinin (HA). The thrombi were initially observed in venules and later, in arterioles. Immunofluorescence study revealed that the HA bound to the thrombi in the microcircul...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF00203305
更新日期:1989-01-01 00:00:00
abstract::Industrial and academic needs for innovation and fundamental research are essential and not widely different. Depending on the industrial setting, research and development (R&D) activities may be more focused on the developmental aspects given the need to ultimately sell useful products. However, one of the biggest di...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章,评审
doi:10.1007/s00430-019-00643-4
更新日期:2020-06-01 00:00:00