Abstract:
:Phosphorylcholine (ChoP) is covalently incorporated into bacterial surface structures, contributing to host mimicry and promoting adhesion to surfaces. Our aims were to determine the frequency of ChoP display among Aggregatibacter actinomycetemcomitans strains, to clarify which surface structures bear ChoP, and whether ChoP-positivity relates to serum killing. The tested oral (N = 67) and blood isolates (N = 27) represented 6 serotypes. Mab TEPC-15 was used for immunoblotting of cell lysates and fractions and for immunofluorescence microscopy of cell surface-bound ChoP. The lysates were denatured with urea for hidden ChoP or treated with proteinase K to test whether it binds to a protein. Three ChoP-positive and two ChoP-negative strains were subjected to serum killing in the presence/absence of CRP and using Ig-depleted serum as complement source. Cell lysates and the first soluble cellular fraction revealed a < 10 kDa band in immunoblots. Among 94 strains, 27 were ChoP positive. No difference was found in the prevalence of ChoP-positive oral (21/67) and blood (6/27) strains. Immunofluorescence microscopy corresponded to the immunoblot results. Proteinase K abolished ChoP reactivity, whereas urea did not change the negative result. The TEPC-15-reactive protein was undetectable in Δflp1 mutant strain. The survival rate of serotype-b strains in serum was 100% irrespective of ChoP, but that of serotype-a was higher in ChoP-positive (85%) than ChoP-negative (71%) strains. The results suggest that a third of rough-colony strains harbor ChoP and that ChoP is attached to fimbrial subunit protein Flp1. It further seems that ChoP-positivity does not enhance but may reduce A. actinomycetemcomitans susceptibility to serum killing.
journal_name
Med Microbiol Immunoljournal_title
Medical microbiology and immunologyauthors
Ihalin R,Zhong D,Karched M,Chen C,Asikainen Sdoi
10.1007/s00430-018-0554-1subject
Has Abstractpub_date
2018-11-01 00:00:00pages
329-338issue
5-6eissn
0300-8584issn
1432-1831pii
10.1007/s00430-018-0554-1journal_volume
207pub_type
杂志文章abstract::EBV-associated polypeptides induced in different Epstein-Barr Virus (EBV)-producing cell lines by the tumor promotor TPA, and from purified EBV particles derived from the same lines were radioactivity labeled and analyzed by immunoprecipitation with human VCA+MA+ sera. In virus-producing cells no significant differenc...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02122703
更新日期:1982-01-01 00:00:00
abstract::We evaluated the long-term effects of the single oral administration of a new CXCR4 antagonist, KRH-3955, on elevation of white blood cell (WBC), neutrophil and lymphocyte counts in normal cynomolgus monkeys. In the monkeys treated with 0, 2, 20, 200 mg/kg of the compound, WBC, neutrophil and lymphocyte counts increas...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-012-0254-1
更新日期:2013-04-01 00:00:00
abstract::The fatal inhalation infection caused by Bacillus anthracis results from a complex pathogenic cycle involving release of toxins by bacteria that germinate from spores. Currently available vaccines against anthrax consist of protective antigen (PA), one of the anthrax toxin components. However, these PA-based vaccines ...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-012-0280-z
更新日期:2013-04-01 00:00:00
abstract::Recently we demonstrated that lack of mecA transcription was responsible for the decreased methicillin resistance phenotype of strongly slime-negative Staphylococcus epidermidis phase variants [Mempel M, Feucht H, Ziehbuhr W, Endres M, Laufs R, Grüter L (1994) Antimicrob Agents Chemother 38: 1251-1255]. In the present...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF00224346
更新日期:1995-10-01 00:00:00
abstract::Mastitis, which is generally described as an inflammation of breast tissue, is a common and debilitating disease which frequently results in the cessation of exclusive breastfeeding and affects up to 33% of lactating women. The condition is a primary cause of decreased milk production and results in organoleptic and n...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章,评审
doi:10.1007/s00430-017-0532-z
更新日期:2018-04-01 00:00:00
abstract::Antibody to recombinant hepatitis C virus (HCV) protein C100 (anti-C100) was measured for a period of 6 months by enzyme immunoassay in nine prospectively followed non A-nonB (NANBH) cases which occurred after cardiac surgery at a hospital in Rio de Janeiro (Brazil). At least seven cases were infected with HCV; four o...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF00191549
更新日期:1992-01-01 00:00:00
abstract::Moraxella catarrhalis is an important pathogen of respiratory and middle ear infections. We previously reported that the attachment of M. catarrhalis to pharyngeal epithelial cells is mediated by ganglioside M2 (GM2). Several sets of adhesins or receptors are involved in such attachment process. In this study, we used...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-002-0109-2
更新日期:2002-05-01 00:00:00
abstract::The antiviral activity of mouse fibroblast interferon against vesicular stomatitis virus was investigated in L-929 mouse fibroblasts and the ganglioside-deficient L-929 mutant cells (ATCC clone NCTC 2071). Although it has been widely reported that gangliosides serve as primary receptors for interferon at the cellular ...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02125527
更新日期:1981-01-01 00:00:00
abstract::Cytomegalovirus vaccine development started in the 1970s with attenuated strains. In the 1980s, one of the strains was shown to be safe and effective in renal transplant patients. Then, attention switched to glycoprotein gB, which was shown to give moderate but transient protection against acquisition of the virus by ...
journal_title:Medical microbiology and immunology
pub_type: 历史文章,杂志文章,评审
doi:10.1007/s00430-015-0388-z
更新日期:2015-06-01 00:00:00
abstract::Illness and death of mice infected by intracerebral inoculation with lymphocytic choriomeningitis virus are pathologic immune phenomena, and mice thus infected were protected when immunosuppressed with cyclosporin A (CSA), although the virus multiplied extensively in all major organs. Concentrations remained high as l...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02123695
更新日期:1985-01-01 00:00:00
abstract::Gangliosides are known to influence cell growth and differentiation. The neolacto series ganglioside IV3NeuAc-nLc4 (2-->3-sialosylparagloboside) is present in members of the monocyte/granulocyte lineage, but is not found in cells that belong to the lymphocyte lineage. In this study we demonstrated that IV3NeuAc-nLc4 i...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s004300050101
更新日期:1999-08-01 00:00:00
abstract::While the antigen structure of yeast cell walls is well known, the immunological cross reactivity of these is often difficult to interpret. The cross reactions of eight yeasts were tested in rabbit hyperimmune sera by the indirect immunofluorescence test. Among the eight species examined were the medically important y...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02120806
更新日期:1979-01-01 00:00:00
abstract::The streptococcal pyrogenic exotoxin B (SpeB) is known to be involved in group A streptococcus (GAS) survival in blood, but the detailed mechanism is not clear. For clarification of this issue, speB isogenic mutants of strains M6 and M49 were constructed by using an integrational plasmid and confirmed by Southern blot...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-005-0001-y
更新日期:2006-06-01 00:00:00
abstract::Different culture conditions, devitalizing treatments, and preservation procedures were tested for the production of protein A-bearing cells of Staphylococcus aureus ATCC 12598. Native cells with the highest IgG-binding activity were obtained after cultivation at 37 degrees C for 24-48 h in enriched media. Devitalizat...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02123683
更新日期:1986-01-01 00:00:00
abstract::TT virus (TTV) is a newly discovered virus from a patient with post-transfusion hepatitis. We investigated the frequency and pathogenesis of TTV infection in children. A semi-nested PCR assay was used to amplify TTV-DNA in serum samples from 254 ambulatory children without liver disease, 20 with hepatitis of unknown e...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s004300050109
更新日期:1999-11-01 00:00:00
abstract::Pteridine derivatives neopterin and 7,8-dihydroneopterin are produced by human macrophages and dendritic cells upon stimulation with interferon-gamma (IFN-gamma) and therefore become detectable in increased amounts in humans during cell-mediated (Th1-type) immune response. Compounds produced upon influence of cytokine...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-006-0025-y
更新日期:2007-03-01 00:00:00
abstract::The rapid and correct identification of pathogens is of paramount importance for the treatment of patients with invasive infections. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) can speed up the identification of bacteria and fungi and has quickly been embraced by medical...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-013-0319-9
更新日期:2014-04-01 00:00:00
abstract::A coupled luminescent method (CLM) based on glyceraldehyde-3-phosphate dehydrogenase released from injured target cells was used to evaluate the cytotoxicity of antigen-specific HLA class I-restricted CTLs. In contrast to established methods, CLM does not require the pretreatment of target cells with radioactive or to...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-009-0126-5
更新日期:2009-11-01 00:00:00
abstract::This is a summary of the recent work on some glycosidases of sand flies and their Leishmania parasites. Glycosidases catalyze the hydrolysis of complex sugar subunits of polysaccharides into simple sugars. Leishmania major parasites secrete chitinase and N-acetylglucosaminase, which enables them to survive in the gut ...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章,评审
doi:10.1007/s004300100079
更新日期:2001-11-01 00:00:00
abstract::Gliosis of glial fibrillary acidic protein (GFAP) associated astrocytes is considered to be one of the hallmarks of transmissible spongiform encephalopathies (TSEs). In the present study, remarkable GFAP-PrP(Sc) or GFAP-PrP(C) complexes were separately detected in the brain homogenates of 263 K (Scrapie)-infected or n...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-007-0071-0
更新日期:2008-12-01 00:00:00
abstract::Immunodominant proteins are variable in molecular and antigenic structure among different genospecies of Borrelia burgdorferi sensu lato. We have recently developed an immunoblot using five recombinant antigens: the chromosomal-encoded B. burgdorferi proteins p100, the flagellin and an internal flagellin fragment ther...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF00193630
更新日期:1994-02-01 00:00:00
abstract::Cytotoxic effects of a leukocidin from Fusobacterium necrophorum were demonstrated on bovine hepatic cells. The cytotoxic response was dose-dependent and could be inhibited by homologous antiserum. Scanning electron microscopy revealed damaged hepatic cell surface. These findings indicated a pathogenic role of the leu...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF00190308
更新日期:1988-01-01 00:00:00
abstract::In the present study, synthetic peptides (EENVEHDA)2 [(oc)2] and (DDEHVEEPTVA)2 [(un)2] of ring-infected erythrocyte surface antigen (RESA) of Plasmodlium filciparum were linked with palmitic acid and entrapped in immunostimulating complexes (ISCOMs). The immunogenicity of the peptide(s) and mixture of peptides were s...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s004300000042
更新日期:2000-11-01 00:00:00
abstract::The effect of immune bovine lactoserum (BLS) antipolyvalent enteropathogenic Escherichia coli on bacterial growth, viability and bacteria-induced fluid accumulation was examined in rabbit ileal loops. Human enteropathogenic E. coli strains 0125:K70 (B15), 0111:K58 (B4) and 055:K59 (B5) (1-3 X 10(9) per inoculum) induc...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02123571
更新日期:1975-12-30 00:00:00
abstract::Therapeutic vaccination for the treatment of chronic hepatitis B has thus far been unsatisfactory. In this review, we discuss potential new therapeutic vaccination strategies and other immunotherapeutic approaches that aim to achieve efficient restoration of HBV immunity in chronically infected patients. ...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章,评审
doi:10.1007/s00430-014-0377-7
更新日期:2015-02-01 00:00:00
abstract::Both aging and treated human immunodeficiency virus (HIV) infection are characterized by low-level chronic inflammation and immune activation which contribute to the development of age-related diseases, frailty, and early mortality. Chronic cytomegalovirus (CMV) infection is highly prevalent in older adults and HIV-in...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章,评审
doi:10.1007/s00430-019-00591-z
更新日期:2019-08-01 00:00:00
abstract::The stimulatory effect of lipopolysaccharide (LPS) on human macrophages was found to be neutralized by the detergent octylglucoside (OG). Both macrophage stimulation and reactivity in a limulus amebocyte lysate test were suppressed by suspension of LPS in OG at concentrations between 0.25 and 2.5 mM, whereas other sti...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s430-001-8019-2
更新日期:2001-04-01 00:00:00
abstract::Pertussis Toxin (PTx) is one of the most important virulence factors of Bordetella pertussis, the cause of whooping cough. Therefore, the inactivated toxin is an obligatory constituent of acellular pertussis vaccines. It is described in the literature that both native PTx and recombinant Pertussis Toxin (PTg) activate...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-012-0238-1
更新日期:2012-08-01 00:00:00
abstract::The improved Auxotab Enteric I System was re-evaluated and found to correlate 98% with conventional methods for the identification of Enterobacteriaceae. Possible Reasons for previous poor results with this system are discussed. ...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02121009
更新日期:1975-07-02 00:00:00
abstract::Human cytomegalovirus (HCMV) infection modulates the host cell cycle to create an environment that is optimal for viral gene expression, DNA replication, and production of infectious virus. The virus mostly infects quiescent cells and thus must push the cell into G1 phase of the cell cycle to co-opt the cellular mecha...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章,评审
doi:10.1007/s00430-015-0396-z
更新日期:2015-06-01 00:00:00