Abstract:
:Different culture conditions, devitalizing treatments, and preservation procedures were tested for the production of protein A-bearing cells of Staphylococcus aureus ATCC 12598. Native cells with the highest IgG-binding activity were obtained after cultivation at 37 degrees C for 24-48 h in enriched media. Devitalization by ethanol, 1-propanol, formaldehyde, glutardialdehyde, chloramine T, and by heat, reduced the protein A activity depending on the duration of treatment. The protein A content of devitalized cells were best preserved by storage at -20 degrees C or by lyophilization. Staphylococcal preparations with a stable IgG-binding activity of 20 mg/g bacteria, which are very suitable for coagglutination tests, were produced by culture in TSB medium, followed by devitalization with 1-propanol and lyophilization.
journal_name
Med Microbiol Immunoljournal_title
Medical microbiology and immunologyauthors
Ansorg RA,Zarifoglu FIdoi
10.1007/BF02123683subject
Has Abstractpub_date
1986-01-01 00:00:00pages
305-12issue
6eissn
0300-8584issn
1432-1831journal_volume
174pub_type
杂志文章abstract::A 33-kDa protein (TB33) was isolated from a delipidated cell sonicate (CS) of Mycobacterium tuberculosis H37Rv (grown in Middlebrook 7H9 broth supplemented with glucose) using immobilized metal affinity chromatography (IMAC) on a nickel-nitrilotriacetic acid (Ni-NTA) column. TB33 could not be isolated from the culture...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s004300050025
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abstract::Antibodies against the human coronavirus OC43 in human sera were measured by haemagglutination inhibition (HI), complement fixation (CF), and radial diffusion-haemolysis in gel (HIG) techniques. The apparent HI titres in a fraction of sera with no antibodies detectable by the two other methods were found to be reduced...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02121147
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abstract::Antibody to recombinant hepatitis C virus (HCV) protein C100 (anti-C100) was measured for a period of 6 months by enzyme immunoassay in nine prospectively followed non A-nonB (NANBH) cases which occurred after cardiac surgery at a hospital in Rio de Janeiro (Brazil). At least seven cases were infected with HCV; four o...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
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journal_title:Medical microbiology and immunology
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更新日期:2002-05-01 00:00:00
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章
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abstract::Cytotoxic effects of a leukocidin from Fusobacterium necrophorum were demonstrated on bovine hepatic cells. The cytotoxic response was dose-dependent and could be inhibited by homologous antiserum. Scanning electron microscopy revealed damaged hepatic cell surface. These findings indicated a pathogenic role of the leu...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF00190308
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abstract::In a randomized, placebo-controlled trial in Ghana, 67 onchocerciasis patients received 200-mg/day doxycycline for 4-6 weeks, followed by ivermectin (IVM) after 6 months. After 6-27 months, efficacy was evaluated by onchocercoma histology, PCR and microfilariae determination. Administration of doxycycline resulted in ...
journal_title:Medical microbiology and immunology
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更新日期:2008-09-01 00:00:00
abstract::Therapeutic vaccination for the treatment of chronic hepatitis B has thus far been unsatisfactory. In this review, we discuss potential new therapeutic vaccination strategies and other immunotherapeutic approaches that aim to achieve efficient restoration of HBV immunity in chronically infected patients. ...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章,评审
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abstract::Upon infection of human T-cell leukemia virus type I (HTLV-I)-carrying human T-cell lines such as MT-4, HTLV-III, a probable etiologic agent of acquired immune deficiency syndrome (AIDS) caused fast and strong cytopathic effects leading ultimately to the death of the cells. Such effects were preceded by the rapid indu...
journal_title:Medical microbiology and immunology
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journal_title:Medical microbiology and immunology
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abstract::IgG subclass antibodies to Pseudomonas aeruginosa outer membrane proteins (OMP) were investigated in serum from cystic fibrosis (CF) patients by immunoblotting and enzyme-linked immunosorbent assay (ELISA). Fifteen patients (eight in good and seven in poor clinical condition) have been followed for an average of 13 ye...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF00191546
更新日期:1992-01-01 00:00:00
abstract::Six colonies producing antibodies were obtained by fusing mouse myeloma SP2-O cells with spleen cells from mice immunized with cytomegalovirus Davis strain. Among 88 surviving clones, 29 produced antibodies detectable by immunofluorescence on infected MRC5 cells and 2 others produced neutralizing antibodies against a ...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02123803
更新日期:1983-01-01 00:00:00
abstract::Earlier we reported that vaccination of leprosy patients with Mycobacterium w induces an immune response directed predominantly against low molecular weight antigens. One of these antigens, with a molecular mass of 30-kDa, was recognized by a majority of the vaccinated subjects as well as the tuberculoid leprosy patie...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF00579623
更新日期:1993-11-01 00:00:00
abstract::We assessed whether the intracellular bacterium Chlamydia pneumoniae was present in post-mortem brain samples from patients with and without late-onset Alzheimer's disease (AD), since some indirect evidence seems to suggest that infection with the organism might be associated with the disease. Nucleic acids prepared f...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s004300050071
更新日期:1998-06-01 00:00:00
abstract::Convulsions and encephalopathy are common complications of Shiga toxin (Stx)-producing Shigella and enterohemorrhagic Escherichia coli infections. In previous studies, we demonstrated that Stx and lipopolysaccharide (LPS) act in concert to enhance mice sensitivity to pentylenetetrazole (PTZ)-induced seizures via mecha...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-001-0108-8
更新日期:2002-03-01 00:00:00
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journal_title:Medical microbiology and immunology
pub_type: 历史文章,杂志文章,评审
doi:10.1007/s00430-018-0566-x
更新日期:2019-02-01 00:00:00
abstract::The new QuantiFERON-TB Gold Plus employs modified peptides optimized to elicit an IFNγ response from CD8+ cytotoxic T lymphocytes in addition to CD4+ T cells. With a view to improve the difficult identification of TB cases, we assessed the combination of two specific immunological markers comprising IFNγ secretion and...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-018-00576-4
更新日期:2019-04-01 00:00:00
abstract::The aroB gene from Helicobacter pylori strain P1 was cloned and further characterized by sequence analysis and by functional complementation of the aroB mutation in Escherichia coli. The aroB gene encodes the enzyme 3-dehydroquinate synthase which catalyzes one of the early steps in the shikimate pathway. This pathway...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
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更新日期:1997-10-01 00:00:00
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02120767
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journal_title:Medical microbiology and immunology
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journal_title:Medical microbiology and immunology
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更新日期:2015-04-01 00:00:00
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journal_title:Medical microbiology and immunology
pub_type: 临床试验,杂志文章
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更新日期:1977-05-18 00:00:00
abstract::We have studied the incidence of enterotoxigenic and enteropathogenic Escherichia coli strains associated with infant diarrhoeal disease in Galicia (North-west Spain). During a period of 9 months we isolated heat-labile enterotoxin-positive strains in 2.1% of children with diarrhoea examined, whereas the production of...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02123802
更新日期:1983-01-01 00:00:00
abstract::A sentinel surveillance system of the public health service for acute respiratory diseases (ARD), especially influenza, has been realized in the Free State of Saxony of Germany for 25 years. The epidemiological sentinel is based on weekly registration of morbidity and mathematical-statistical calculations. For a micro...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-002-0136-z
更新日期:2002-12-01 00:00:00
abstract::Oral candidiasis (OC) is a frequent oral manifestation of HIV infection, is a marker disease and occurs as a pseudomembranous, erythematous or rarely hyperplastic variant; angular cheilitis is also seen. Candida albicans is frequently isolated but other species such as C. krusei and C. dublienensis are emerging. Resis...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章,评审
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更新日期:2003-08-01 00:00:00
abstract::Various strains of Helicobacter pylori were able to lyse erythrocytes from sheep, horse, and human when grown on blood agar. The hemolysis did not depend on the production of the vacuolating cytotoxin VacA as demonstrated by the hemolytic behavior of an isogenic vacA-negative mutant strain. The hemolytic activity coul...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s004300050062
更新日期:1998-03-01 00:00:00
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02121133
更新日期:1978-11-17 00:00:00
abstract::Enterocytozoon bieneusi is emerging as an important cause of chronic diarrhoea in AIDS patients. Its reservoirs and transmission patterns are unknown. In this study, we have examined E. bieneusi sequences from four Rhesus macaques of different origin, which were kept at one animal facility. The sequences were identica...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-005-0240-y
更新日期:2005-08-01 00:00:00
abstract::Gangliosides are known to influence cell growth and differentiation. The neolacto series ganglioside IV3NeuAc-nLc4 (2-->3-sialosylparagloboside) is present in members of the monocyte/granulocyte lineage, but is not found in cells that belong to the lymphocyte lineage. In this study we demonstrated that IV3NeuAc-nLc4 i...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s004300050101
更新日期:1999-08-01 00:00:00