Transcripts expressed in cytomegalovirus latency coding for an antigenic IE/E phase peptide that drives "memory inflation".

Abstract:

:Roizman's definition of herpesviral latency, which applies also to cytomegaloviruses (CMVs), demands maintenance of reactivation-competent viral genomes after clearance of productive infection. It is more recent understanding that failure to complete the productive viral cycle for virus assembly and release does not imply viral gene silencing at all genetic loci and all the time. It rather appears that CMV latency is transcriptionally "noisy" in that silenced viral genes get desilenced from time to time in a stochastic manner, leading to "transcripts expressed in latency" (TELs). If a TEL happens to code for a protein that contains a CD8 T cell epitope, protein processing can lead to the presentation of the antigenic peptide and restimulation of cognate CD8 T cells during latency. This mechanism is discussed as a potential driver of epitope-selective accumulation of CD8 T cells over time, a phenomenon linked to CMV latency and known as "memory inflation" (MI). So far, expression of an epitope-encoding TEL was shown only for the major immediate-early (MIE) gene m123/ie1 of murine cytomegalovirus (mCMV), which codes for the prototypic MI-driving antigenic peptide YPHFMPTNL that is presented by the MHC class-I molecule Ld. The only known second MI-driving antigenic peptide of mCMV in the murine MHC haplotype H-2d is AGPPRYSRI presented by the MHC-I molecule Dd. This peptide is very special in that it is encoded by the early (E) phase gene m164 and by an overlapping immediate-early (IE) transcript governed by a promoter upstream of m164. If MI is driven by presentation of TEL-derived antigenic peptides, as the hypothesis says, one should find corresponding TELs. We show here that E-phase and IE-phase transcripts that code for the MI-driving antigenic peptide AGPPRYSRI are independently and stochastically expressed in latently infected lungs.

journal_name

Med Microbiol Immunol

authors

Renzaho A,Schmiedeke JK,Griessl M,Kühnapfel B,Seckert CK,Lemmermann NAW

doi

10.1007/s00430-019-00615-8

subject

Has Abstract

pub_date

2019-08-01 00:00:00

pages

439-446

issue

3-4

eissn

0300-8584

issn

1432-1831

pii

10.1007/s00430-019-00615-8

journal_volume

208

pub_type

杂志文章
  • HIV-1 subtypes and drug resistance profiles in a cohort of heterosexual patients in Istanbul, Turkey.

    abstract::Turkey is seeing a steady rise in rates of HIV infection in the country. The number of individuals with HIV/AIDS was greater than 7000 in 2014 according to data released by the Ministry of Health, and heterosexual contacts were reported to be the main transmission routes. Istanbul has the highest number of reported ca...

    journal_title:Medical microbiology and immunology

    pub_type: 杂志文章

    doi:10.1007/s00430-015-0419-9

    authors: Köksal MO,Beka H,Lübke N,Verheyen J,Eraksoy H,Cagatay A,Kaiser R,Akgül B,Agacfidan A

    更新日期:2015-08-01 00:00:00

  • Clinical utility of measuring Epstein-Barr virus-specific cell-mediated immunity after HSCT in addition to virological monitoring: results from a prospective study.

    abstract::Lack of virus-specific cell-mediated immunity (CMI) is associated with worse viral infection outcome in hematopoietic stem cell transplantation (HSCT). We aimed to evaluate the role of immunological monitoring of Epstein-Barr virus (EBV) infection in addition to virological one in 33 adult and 18 pediatric allogeneic ...

    journal_title:Medical microbiology and immunology

    pub_type: 杂志文章

    doi:10.1007/s00430-019-00629-2

    authors: Chiereghin A,Piccirilli G,Belotti T,Prete A,Bertuzzi C,Gibertoni D,Gabrielli L,Turello G,Borgatti EC,Barbato F,Sessa M,Arpinati M,Bonifazi F,Lazzarotto T

    更新日期:2019-12-01 00:00:00

  • Modulation of the lung local immune response by systemic immunization.

    abstract::The present study describes the respiratory immune response of mice to locally administered antigen, and the modulation of this response by systemic immunization. Intranasal immunization of mice with the A/PR/8/34 strain of influenza virus evoked local antibody response of the IgA type. The titer of the IgA antibodies...

    journal_title:Medical microbiology and immunology

    pub_type: 杂志文章

    doi:10.1007/BF02121133

    authors: Zakay-Rones Z,Hadas O,Levy R

    更新日期:1978-11-17 00:00:00

  • Simultaneous analysis of multiple T helper subsets in leprosy reveals distinct patterns of Th1, Th2, Th17 and Tregs markers expression in clinical forms and reactional events.

    abstract::Leprosy is a chronic infectious disease caused by Mycobacterium leprae. Previous studies have demonstrated that the difference among clinical forms of leprosy can be associated with the immune response of patients, mainly by T helper (Th) and regulatory T cells (Tregs). Then, aiming at clarifying the immune response, ...

    journal_title:Medical microbiology and immunology

    pub_type: 杂志文章

    doi:10.1007/s00430-017-0519-9

    authors: Azevedo MCS,Marques H,Binelli LS,Malange MSV,Devides AC,Silva EA,Fachin LRV,Ghidella CC,Soares CT,Garlet GP,Rosa PS,Belone AFF,Trombone APF

    更新日期:2017-12-01 00:00:00

  • Polypeptide composition of mumps virus.

    abstract::The Enders strain of mumps virus grown in ovo was purified by differential and equilibrium sucrose gradient sedimentation. Purified virus contained seven polypeptides of mol. wts 68,000, 66,000, 61,000, 54,000, 52,000, 49,000, 47,000. Nucleocapsids isolated from DOC-treated virus contained two polypeptides of mol. wts...

    journal_title:Medical microbiology and immunology

    pub_type: 杂志文章

    doi:10.1007/BF02125509

    authors: Huppertz HI,Hall WW,ter Meulen V

    更新日期:1977-12-27 00:00:00

  • Isolation of a 33-kDa protein antigen from delipidified Mycobacterium tuberculosis H37Rv.

    abstract::A 33-kDa protein (TB33) was isolated from a delipidated cell sonicate (CS) of Mycobacterium tuberculosis H37Rv (grown in Middlebrook 7H9 broth supplemented with glucose) using immobilized metal affinity chromatography (IMAC) on a nickel-nitrilotriacetic acid (Ni-NTA) column. TB33 could not be isolated from the culture...

    journal_title:Medical microbiology and immunology

    pub_type: 杂志文章

    doi:10.1007/s004300050025

    authors: Deshpande RG,Khan MB,Bhat DA,Navalkar RG

    更新日期:1996-11-01 00:00:00

  • Immune response to a recombinant fragment of the CagA protein of Helicobacter pylori in blood donors and patients with gastric cancer: relation to ABO(H) blood group phenotype, stage of the disease and tumor morphology.

    abstract::IgG immune response to CagA was evaluated by enzyme-linked imunosorbent assay (ELISA) using a recombinant fragment of CagA as antigen in 171 patients with gastric cancer and 298 blood donors to determine whether it could be related to the ABO(H) blood group phenotype, stage of cancer or tumor morphology. The CagA-ELIS...

    journal_title:Medical microbiology and immunology

    pub_type: 杂志文章

    doi:10.1007/s004300050097

    authors: Klaamas K,Kurtenkov O,Covacci A,Lipping A,Wadström T

    更新日期:1999-05-01 00:00:00

  • Variable degree of slime production is linked to different levels of beta-lactam susceptibility in Staphylococcus epidermidis phase variants.

    abstract::Recently we demonstrated that lack of mecA transcription was responsible for the decreased methicillin resistance phenotype of strongly slime-negative Staphylococcus epidermidis phase variants [Mempel M, Feucht H, Ziehbuhr W, Endres M, Laufs R, Grüter L (1994) Antimicrob Agents Chemother 38: 1251-1255]. In the present...

    journal_title:Medical microbiology and immunology

    pub_type: 杂志文章

    doi:10.1007/BF00224346

    authors: Mempel M,Müller E,Hoffmann R,Feucht H,Laufs R,Grüter L

    更新日期:1995-10-01 00:00:00

  • Influence of Pseudomonas aeruginosa quorum sensing signal molecule N-(3-oxododecanoyl) homoserine lactone on mast cells.

    abstract::Quorum sensing system is a cell-to-cell communication system that plays a pivotal role in virulence expression in bacteria. Recent advances have demonstrated that the Pseudomonas aeruginosa quorum sensing molecule, N-3-oxododecanoyl homoserine lactone (3OC(12)-HSL), exerts effects on mammalian cells and modulates host...

    journal_title:Medical microbiology and immunology

    pub_type: 杂志文章

    doi:10.1007/s00430-009-0111-z

    authors: Li H,Wang L,Ye L,Mao Y,Xie X,Xia C,Chen J,Lu Z,Song J

    更新日期:2009-05-01 00:00:00

  • The biological function of sand fly and Leishmania glycosidases.

    abstract::This is a summary of the recent work on some glycosidases of sand flies and their Leishmania parasites. Glycosidases catalyze the hydrolysis of complex sugar subunits of polysaccharides into simple sugars. Leishmania major parasites secrete chitinase and N-acetylglucosaminase, which enables them to survive in the gut ...

    journal_title:Medical microbiology and immunology

    pub_type: 杂志文章,评审

    doi:10.1007/s004300100079

    authors: Jacobson RL,Schlein Y,Eisenberger CL

    更新日期:2001-11-01 00:00:00

  • Activation of cord blood myeloid dendritic cells by Trypanosoma cruzi and parasite-specific antibodies, proliferation of CD8+ T cells, and production of IFN-γ.

    abstract::We previously reported that Trypanosoma cruzi, the agent of Chagas disease, induces in congenitally infected fetuses a strong, adult-like parasite-specific CD8(+) T cell response producing IFN-γ (Hermann et al. in Blood 100:2153-2158, 2002). This suggests that the parasite is able to overcome the immaturity of neonata...

    journal_title:Medical microbiology and immunology

    pub_type: 杂志文章

    doi:10.1007/s00430-011-0217-y

    authors: Rodriguez P,Carlier Y,Truyens C

    更新日期:2012-05-01 00:00:00

  • Acute respiratory diseases/influenza sentinel 2000/2001.

    abstract::A sentinel surveillance system of the public health service for acute respiratory diseases (ARD), especially influenza, has been realized in the Free State of Saxony of Germany for 25 years. The epidemiological sentinel is based on weekly registration of morbidity and mathematical-statistical calculations. For a micro...

    journal_title:Medical microbiology and immunology

    pub_type: 杂志文章

    doi:10.1007/s00430-002-0136-z

    authors: Bigl S,Briem I,Drechsler R,Kluge D,Müller L,Nowotnik G

    更新日期:2002-12-01 00:00:00

  • Minimal attachment killing (MAK): a versatile method for susceptibility testing of attached biofilm-positive and -negative Staphylococcus epidermidis.

    abstract::Due to its ability to attach to polymeric surfaces Staphylococcus epidermidis is a common pathogen in chronic, medical device-associated infections. Attached S. epidermidis displays reduced susceptibility against a variety of antimicrobial substances, and little correlation between standard susceptibility test results...

    journal_title:Medical microbiology and immunology

    pub_type: 杂志文章

    doi:10.1007/s00430-002-0125-2

    authors: Knobloch JK,Von Osten H,Horstkotte MA,Rohde H,Mack D

    更新日期:2002-10-01 00:00:00

  • Hemolytic properties and riboflavin synthesis of Helicobacter pylori: cloning and functional characterization of the ribA gene encoding GTP-cyclohydrolase II that confers hemolytic activity to Escherichia coli.

    abstract::Various strains of Helicobacter pylori were able to lyse erythrocytes from sheep, horse, and human when grown on blood agar. The hemolysis did not depend on the production of the vacuolating cytotoxin VacA as demonstrated by the hemolytic behavior of an isogenic vacA-negative mutant strain. The hemolytic activity coul...

    journal_title:Medical microbiology and immunology

    pub_type: 杂志文章

    doi:10.1007/s004300050062

    authors: Bereswill S,Fassbinder F,Völzing C,Covacci A,Haas R,Kist M

    更新日期:1998-03-01 00:00:00

  • Viral tropisms in mouse brain cell cultures.

    abstract::Fourteen-day-old cultures of dissociated newborn mouse brain cells were infected separately with different strains of vaccinia virus and a strain of measles virus. Using the indirect immunofluorescence technique we found that under the experimental conditions in these cultures both measles and the neurotropic strain o...

    journal_title:Medical microbiology and immunology

    pub_type: 杂志文章

    doi:10.1007/BF02298200

    authors: Beranek CF,Schäfer R,Bologa L,Herschkowitz N

    更新日期:1982-01-01 00:00:00

  • The history of vaccination against cytomegalovirus.

    abstract::Cytomegalovirus vaccine development started in the 1970s with attenuated strains. In the 1980s, one of the strains was shown to be safe and effective in renal transplant patients. Then, attention switched to glycoprotein gB, which was shown to give moderate but transient protection against acquisition of the virus by ...

    journal_title:Medical microbiology and immunology

    pub_type: 历史文章,杂志文章,评审

    doi:10.1007/s00430-015-0388-z

    authors: Plotkin S

    更新日期:2015-06-01 00:00:00

  • Live or let die: manipulation of cellular suicide programs by murine cytomegalovirus.

    abstract::Cytomegaloviruses (CMVs) are large double-stranded DNA viruses that replicate slowly and cause life-long persisting infections in their hosts. To achieve this, the CMVs had to evolve numerous countermeasures against innate and adaptive immune responses. Induction of programmed cell death is one important host defense ...

    journal_title:Medical microbiology and immunology

    pub_type: 杂志文章,评审

    doi:10.1007/s00430-012-0264-z

    authors: Handke W,Krause E,Brune W

    更新日期:2012-11-01 00:00:00

  • Kaposi's sarcoma-associated herpesvirus seroprevalence in selected german patients: evaluation by different test systems.

    abstract::A total of 603 serum samples obtained from 12 different patient and control groups, including potentially cross-reactive sera, were tested for the presence of antibodies against Kaposi's sarcoma-associated herpesvirus (KSHV, HHV-8). The assays used were an inhouse immunofluorescence test (IFT) employing latent KSHV an...

    journal_title:Medical microbiology and immunology

    pub_type: 杂志文章

    doi:10.1007/s00430-001-0098-6

    authors: Preiser W,Szép NI,Lang D,Doerr HW,Rabenau HF

    更新日期:2001-12-01 00:00:00

  • Experience with the clinical development of influenza vaccines for potential pandemics.

    abstract::During normal interpandemic influenza seasons, immune responses to vaccines are quite predictable and meet the licensing criteria of the European Union (EU) Committee for Proprietary Medicinal Products (CPMP). In a pandemic situation, large sections, if not all of the community will be immunologically naïve and theref...

    journal_title:Medical microbiology and immunology

    pub_type: 杂志文章

    doi:10.1007/s00430-002-0146-x

    authors: Wood JM,Nicholson KG,Stephenson I,Zambon M,Newman RW,Major DL,Podda A

    更新日期:2002-12-01 00:00:00

  • The influence of the intestinal microflora on the relative thymus weight.

    abstract::Oral treatment of mice with a combination of non-absorbable broad spectrum antibiotics at a dose level high enough to sterilize the digestive tract within five days of treatment, has been found to have a significant negative effect on the relative weight of the thymus. From the age of 12 weeks onwards, the mean relati...

    journal_title:Medical microbiology and immunology

    pub_type: 杂志文章

    doi:10.1007/BF02123870

    authors: van der Waaij D

    更新日期:1986-01-01 00:00:00

  • Four hour-test for the identification of Enterobacteriaceae.

    abstract::A set of 12 rapid biochemical tests--lysinedecarboxylase, ornithinedecarboxylase, beta-galactosidase, urease, hydrogensulphide, indole, acetoin, deoxyribonuclease, esculin, mannitol, raffinose and sorbitol--were selected from an original set of 13 tests and were found to give 98% accurate reactions within 4 hrs of inc...

    journal_title:Medical microbiology and immunology

    pub_type: 杂志文章

    doi:10.1007/BF02122710

    authors: Nord C-E,Linberg AA,Dahlbäck A

    更新日期:1975-09-19 00:00:00

  • The impact of KIR-HLA genotype on hepatitis B virus clearance in Iranian infected individuals.

    abstract::Killer cell immunoglobulin like receptors (KIRs) have a principal role in regulating the effector functions of NK cells, particularly in viral infections. The major ligands for KIRs are human leukocyte antigen (HLA) class I molecules. The aim of this study is to investigate the possible association of KIR genes, their...

    journal_title:Medical microbiology and immunology

    pub_type: 杂志文章

    doi:10.1007/s00430-017-0522-1

    authors: Shah-Hosseini A,Jafari M,Mohammadi A,Sanaei R,Alavian SM,Doosti-Irani A,Nooradeh Keykavousi M,Tajik N

    更新日期:2017-12-01 00:00:00

  • Demonstration of Borna disease virus-specific RNA in secretions of naturally infected horses by the polymerase chain reaction.

    abstract::The presence of Borna disease virus (BDV)-specific RNA was traced by the reverse transcriptase-polymerase chain reaction in conjunctival fluid, nasal secretions and saliva of horses which were seropositive but did not have any history of clinical Borna disease. Positive reactions encompassed sequences encoding the p24...

    journal_title:Medical microbiology and immunology

    pub_type: 杂志文章

    doi:10.1007/BF00191945

    authors: Richt JA,Herzog S,Haberzettl K,Rott R

    更新日期:1993-12-01 00:00:00

  • Performance of two MALDI-TOF MS systems for the identification of yeasts isolated from bloodstream infections and cerebrospinal fluids using a time-saving direct transfer protocol.

    abstract::The rapid and correct identification of pathogens is of paramount importance for the treatment of patients with invasive infections. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) can speed up the identification of bacteria and fungi and has quickly been embraced by medical...

    journal_title:Medical microbiology and immunology

    pub_type: 杂志文章

    doi:10.1007/s00430-013-0319-9

    authors: Hamprecht A,Christ S,Oestreicher T,Plum G,Kempf VA,Göttig S

    更新日期:2014-04-01 00:00:00

  • Human T helper cells reactive with somatic bacterial antigens belong to the Th1 subset.

    abstract::The aim of this study was to characterize the cytokine secretion patterns of human T helper cells from healthy donors reactive with somatic antigens from various bacteria, the nematode Anisakis and tetanus toxoid. From the peripheral blood of four healthy donors we have established 70 T cell lines reactive with antige...

    journal_title:Medical microbiology and immunology

    pub_type: 杂志文章

    doi:10.1007/BF00196051

    authors: Schlaak JF,Nieder P,Meyer zum Büschenfelde KH,Fleischer B

    更新日期:1994-07-01 00:00:00

  • Internalization of human rhinovirus 14 into HeLa and ICAM-1-transfected BHK cells.

    abstract::Virus adsorption and uptake of human rhinovirus 14 (HRV14) were studied with HeLa cells and baby hamster kidney (BHK) cells which were transfected with the HRV14 receptor intercellular adhesion molecule-1 (ICAM-1). Transmission electron microscopy of HeLa cells revealed that HRV14 was internalized via clathrin-coated ...

    journal_title:Medical microbiology and immunology

    pub_type: 杂志文章

    doi:10.1007/s004300050039

    authors: Grunert HP,Wolf KU,Langner KD,Sawitzky D,Habermehl KO,Zeichhardt H

    更新日期:1997-06-01 00:00:00

  • Involvement of polyphosphate kinase in virulence and stress tolerance of uropathogenic Proteus mirabilis.

    abstract::Proteus mirabilis (P. mirabilis), a gram-negative enteric bacterium, frequently causes urinary tract infections. Many virulence factors of uropathogenic P. mirabilis have been identified, including urease, flagella, hemolysin and fimbriae. However, the functions of polyphosphate kinase (PPK), which are related to the ...

    journal_title:Medical microbiology and immunology

    pub_type: 杂志文章

    doi:10.1007/s00430-015-0430-1

    authors: Peng L,Jiang Q,Pan JY,Deng C,Yu JY,Wu XM,Huang SH,Deng XY

    更新日期:2016-04-01 00:00:00

  • Wolbachia endobacteria depletion by doxycycline as antifilarial therapy has macrofilaricidal activity in onchocerciasis: a randomized placebo-controlled study.

    abstract::In a randomized, placebo-controlled trial in Ghana, 67 onchocerciasis patients received 200-mg/day doxycycline for 4-6 weeks, followed by ivermectin (IVM) after 6 months. After 6-27 months, efficacy was evaluated by onchocercoma histology, PCR and microfilariae determination. Administration of doxycycline resulted in ...

    journal_title:Medical microbiology and immunology

    pub_type: 杂志文章,随机对照试验

    doi:10.1007/s00430-007-0062-1

    authors: Hoerauf A,Specht S,Büttner M,Pfarr K,Mand S,Fimmers R,Marfo-Debrekyei Y,Konadu P,Debrah AY,Bandi C,Brattig N,Albers A,Larbi J,Batsa L,Taylor MJ,Adjei O,Büttner DW

    更新日期:2008-09-01 00:00:00

  • Inducing protective antibodies against ring-infected erythrocyte surface peptide antigen of Plasmodium falciparum using immunostimulating complex (ISCOMs) delivery.

    abstract::In the present study, synthetic peptides (EENVEHDA)2 [(oc)2] and (DDEHVEEPTVA)2 [(un)2] of ring-infected erythrocyte surface antigen (RESA) of Plasmodlium filciparum were linked with palmitic acid and entrapped in immunostimulating complexes (ISCOMs). The immunogenicity of the peptide(s) and mixture of peptides were s...

    journal_title:Medical microbiology and immunology

    pub_type: 杂志文章

    doi:10.1007/s004300000042

    authors: Chopra N,Biswas S,Thomas B,Sabhnani L,Rao DN

    更新日期:2000-11-01 00:00:00

  • Streptococcus agalactiae CAMP factor/protein B does not bind to human IgG.

    abstract::CAMP factor is an extracellular cytolytic protein produced by Streptococcus agalactiae. CAMP factor has been reported to bind the Fc fragments of immunoglobulin G (IgG) and has therefore also been called protein B, in analogy to protein A of Staphylococcus aureus. We attempted to characterize the interaction of protei...

    journal_title:Medical microbiology and immunology

    pub_type: 杂志文章

    doi:10.1007/s00430-006-0028-8

    authors: El-Huneidi W,Mui R,Zhang TH,Palmer M

    更新日期:2007-06-01 00:00:00