An immunodominant 30-kDa antigen of a candidate anti-leprosy vaccine, Mycobacterium w, shares T and B cell determinants with M. leprae and M. tuberculosis.

Abstract:

:Earlier we reported that vaccination of leprosy patients with Mycobacterium w induces an immune response directed predominantly against low molecular weight antigens. One of these antigens, with a molecular mass of 30-kDa, was recognized by a majority of the vaccinated subjects as well as the tuberculoid leprosy patients and healthy contacts. In the present communication we report further characterization of this antigen. Immunofluorescence and Western blot studies with antibodies raised against this antigen demonstrate that it is associated with the cell surface and has homologues present in M. leprae and M. tuberculosis. Delayed-type hypersensitivity studies carried out in guinea pigs immunized with the 30-kDa antigen show that in addition to sharing B cell determinants, this immunodominant antigen of M. w also shares T cell determinants with M. leprae and M. tuberculosis.

journal_name

Med Microbiol Immunol

authors

Yadava A,Mukherjee R

doi

10.1007/BF00579623

subject

Has Abstract

pub_date

1993-11-01 00:00:00

pages

243-53

issue

5

eissn

0300-8584

issn

1432-1831

journal_volume

182

pub_type

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