Abstract：
:The present study analyses the susceptibility of human bladder-derived cells (HT-1376) to the infection by herpes simplex virus type 2 (HSV-2) and Chlamydia trachomatis, as well as to the adhesiveness of uropathogenic bacteria. HT-1376 cells were efficiently infected by HSV-2 strain 333, as demonstrated by immunofluorescence staining of viral antigens, titration of cytopathic effect, and visualisation by transmission electron microscopy. This cell model was also prone to C. trachomatis (serovar E, Bour strain) replication and to the adherence of clinical uropathogenic isolates of Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris and Enterococcus faecalis. The pre-infection of HT-1376 cells with HSV-2 caused a tenfold increased adherence of an E. coli strain (U1), isolated from a patient affected by severe haemorrhagic cystitis, whereas in HSV-2 pre-infected cells the number of C. trachomatis inclusion bodies was significantly reduced. Our findings indicate that these cells are a suitable in vitro model for studying infection and super-infection of the lower urinary tract by viruses and bacteria.
journal_name
Med Microbiol Immunoljournal_title
Medical microbiology and immunologyauthors
Superti F,Longhi C,Di Biase AM,Tinari A,Marchetti M,Pisani S,Gallinelli C,Chiarini F,Seganti Ldoi
10.1007/s004300100067keywords:
subject
Has Abstractpub_date
2001-09-01 00:00:00pages
201-8issue
4eissn
0300-8584issn
1432-1831journal_volume
189pub_type
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journal_title：Medical microbiology and immunology
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journal_title：Medical microbiology and immunology
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journal_title：Medical microbiology and immunology
pub_type： 杂志文章
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journal_title：Medical microbiology and immunology
pub_type： 杂志文章
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journal_title：Medical microbiology and immunology
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journal_title：Medical microbiology and immunology
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journal_title：Medical microbiology and immunology
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journal_title：Medical microbiology and immunology
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journal_title：Medical microbiology and immunology
pub_type： 杂志文章
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journal_title：Medical microbiology and immunology
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journal_title：Medical microbiology and immunology
pub_type： 杂志文章
doi：10.1007/BF02123296
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journal_title：Medical microbiology and immunology
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journal_title：Medical microbiology and immunology
pub_type： 杂志文章
doi：10.1007/BF02123497
更新日期：1983-01-01 00:00:00
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journal_title：Medical microbiology and immunology
pub_type： 杂志文章,评审
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journal_title：Medical microbiology and immunology
pub_type： 杂志文章
doi：10.1007/BF02123127
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journal_title：Medical microbiology and immunology
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journal_title：Medical microbiology and immunology
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doi：10.1007/BF02123695
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journal_title：Medical microbiology and immunology
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journal_title：Medical microbiology and immunology
pub_type： 杂志文章
doi：10.1007/s004300100070
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journal_title：Medical microbiology and immunology
pub_type： 杂志文章
doi：10.1007/BF02122855
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journal_title：Medical microbiology and immunology
pub_type： 杂志文章
doi：10.1007/s004300050112
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journal_title：Medical microbiology and immunology
pub_type： 杂志文章
doi：10.1007/s00430-019-00631-8
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journal_title：Medical microbiology and immunology
pub_type： 杂志文章
doi：10.1007/s00430-004-0218-1
更新日期：2005-01-01 00:00:00
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journal_title：Medical microbiology and immunology
pub_type： 杂志文章
doi：10.1007/BF02123569
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journal_title：Medical microbiology and immunology
pub_type： 杂志文章
doi：10.1007/s00430-002-0161-y
更新日期：2003-08-01 00:00:00
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journal_title：Medical microbiology and immunology
pub_type： 历史文章,杂志文章,评审
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