Abstract:
:In this study, we presented the construction of a library of expression clones for the herpes simplex virus type 1 (HSV-1) proteome and subcellular localization map of HSV-1 proteins in living cells using yellow fluorescent protein (YFP) fusion proteins. As a result, 21 proteins showed cytoplasmic or subcytoplasmic localization, 16 proteins showed nuclear or subnuclear localization, and others were present both in the nucleus and cytoplasm. Interestingly, most capsid proteins showed enriched or exclusive localization in the nucleus, and most of the envelope proteins showed cytoplasmic localization, suggesting that subcellular localization of the proteins correlated with their functions during virus replication. These results present a subcellular localization map of HSV-1 proteins in living cells, which provide useful information to further characterize the functions of these proteins.
journal_name
Med Microbiol Immunoljournal_title
Medical microbiology and immunologyauthors
Xing J,Wang S,Li Y,Guo H,Zhao L,Pan W,Lin F,Zhu H,Wang L,Li M,Wang L,Zheng Cdoi
10.1007/s00430-010-0175-9subject
Has Abstractpub_date
2011-02-01 00:00:00pages
61-8issue
1eissn
0300-8584issn
1432-1831journal_volume
200pub_type
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