Abstract:
:IgG subclass antibodies to Pseudomonas aeruginosa outer membrane proteins (OMP) were investigated in serum from cystic fibrosis (CF) patients by immunoblotting and enzyme-linked immunosorbent assay (ELISA). Fifteen patients (eight in good and seven in poor clinical condition) have been followed for an average of 13 years with multiple serum samples covering the preinfection, and early and late stages of chronic infection. Laser-scanning densitometry of photographs taken from immunoblots was used to quantify antibody level and compare with ELISA titres. The earliest anti-OMP antibodies to appear were of the IgG1 subclass. There was no significant difference in IgG subclass antibody levels to OMPs between patients in relatively good and poor clinical condition. Data presented indicate a high positive correlation among measurements of IgG subclass antibodies to P. aeruginosa OMPs using ELISA and immunoblotting.
journal_name
Med Microbiol Immunoljournal_title
Medical microbiology and immunologyauthors
Pressler T,Kronborg G,Shand GH,Mansa B,Høiby Ndoi
10.1007/BF00191546keywords:
subject
Has Abstractpub_date
1992-01-01 00:00:00pages
339-49issue
6eissn
0300-8584issn
1432-1831journal_volume
181pub_type
杂志文章abstract::Porphyromonas gingivalis, a periodontal pathogen, is epidemiologically associated with oral squamous cell carcinoma (OSCC). Matrix metalloproteinase 9 (MMP9) which degrades the extracellular matrix and basement membrane components has been implicated in invasion and metastasis of tumor cells. We previously reported th...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-015-0389-y
更新日期:2015-10-01 00:00:00
abstract::Congenital cytomegalovirus (CMV) infection is the leading cause for sensorineural hearing loss and mental retardation in children without genetic diseases worldwide. There is little evidence guiding therapeutic strategies during pregnancy when intrauterine fetal CMV infection is confirmed. We provide a systematic revi...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章,评审
doi:10.1007/s00430-017-0512-3
更新日期:2017-10-01 00:00:00
abstract::During normal interpandemic influenza seasons, immune responses to vaccines are quite predictable and meet the licensing criteria of the European Union (EU) Committee for Proprietary Medicinal Products (CPMP). In a pandemic situation, large sections, if not all of the community will be immunologically naïve and theref...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-002-0146-x
更新日期:2002-12-01 00:00:00
abstract::We have examined 118 oral squamous cell carcinomas, 72 oral leukoplakias, 12 cases of cheilitis and 65 of oral lichen planus for the presence of human papillomavirus (HPV) 6/11, 16 and 18 DNA by PCR/Southern blot hybridization. HPV DNA were found in 51/118 carcinomas (43.2%), in 16/72 (22.2%) leukoplakias, 3/12 (25.0%...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-002-0161-y
更新日期:2003-08-01 00:00:00
abstract::IgG responses are fundamental to adaptive immunity and document immunological memory of previous pathogen encounter. While specific antigen recognition is mediated by the variable F(ab')2 domain of IgG, various effector functions become activated via the constant Fcγ part bridging IgG-opsonized targets to FcγR-express...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-016-0457-y
更新日期:2016-10-01 00:00:00
abstract::We previously reported that Trypanosoma cruzi, the agent of Chagas disease, induces in congenitally infected fetuses a strong, adult-like parasite-specific CD8(+) T cell response producing IFN-γ (Hermann et al. in Blood 100:2153-2158, 2002). This suggests that the parasite is able to overcome the immaturity of neonata...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-011-0217-y
更新日期:2012-05-01 00:00:00
abstract::A sentinel surveillance system of the public health service for acute respiratory diseases (ARD), especially influenza, has been realized in the Free State of Saxony of Germany for 25 years. The epidemiological sentinel is based on weekly registration of morbidity and mathematical-statistical calculations. For a micro...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-002-0136-z
更新日期:2002-12-01 00:00:00
abstract::We assessed whether the intracellular bacterium Chlamydia pneumoniae was present in post-mortem brain samples from patients with and without late-onset Alzheimer's disease (AD), since some indirect evidence seems to suggest that infection with the organism might be associated with the disease. Nucleic acids prepared f...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s004300050071
更新日期:1998-06-01 00:00:00
abstract::Human cytomegalovirus (HCMV) is the most common virus infection in the developing fetus. A fraction of infants infected in utero develop significant life-threatening and organ-threatening disease with over 90% of infected infants exhibiting no clinical evidence of infection in the newborn period. However, about 10% of...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章,评审
doi:10.1007/s00430-015-0399-9
更新日期:2015-06-01 00:00:00
abstract::The DNAs of two kinds of microorganisms from human leprosy lesion, Mycobacterium leprae and Corynebacterium tuberculostearicum (also known as "leprosy-derived corynebacterium" or LDC), have been analysed and compared with the genomes of reference bacteria of the CMN group (genera Corynebacterium, Mycobacterium and Noc...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF00190309
更新日期:1988-01-01 00:00:00
abstract::The endogenous danger signal bradykinin was recently found implicated in the development of immunity against parasites via dendritic cells. We here report an essential role of the B(2) (B(2)R) bradykinin receptor in the early immune response against Listeria infection. Mice deficient in B(2)R (B(2)R(-/-) mice) were sh...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-008-0103-4
更新日期:2009-02-01 00:00:00
abstract::Due to its ability to attach to polymeric surfaces Staphylococcus epidermidis is a common pathogen in chronic, medical device-associated infections. Attached S. epidermidis displays reduced susceptibility against a variety of antimicrobial substances, and little correlation between standard susceptibility test results...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-002-0125-2
更新日期:2002-10-01 00:00:00
abstract::The present study was designed to determine whether tumor induction by 3-methylcholanthrene (MC), a carcinogenic hydrocarbon, can be inhibited by oral administration of Lactobacillus casei strain Shirota (LC). C3H/HeN mice were divided into four groups and assigned to the following treatments: treated with MC and give...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s004300050112
更新日期:1999-12-01 00:00:00
abstract::In this study, we presented the construction of a library of expression clones for the herpes simplex virus type 1 (HSV-1) proteome and subcellular localization map of HSV-1 proteins in living cells using yellow fluorescent protein (YFP) fusion proteins. As a result, 21 proteins showed cytoplasmic or subcytoplasmic lo...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-010-0175-9
更新日期:2011-02-01 00:00:00
abstract::The fatal inhalation infection caused by Bacillus anthracis results from a complex pathogenic cycle involving release of toxins by bacteria that germinate from spores. Currently available vaccines against anthrax consist of protective antigen (PA), one of the anthrax toxin components. However, these PA-based vaccines ...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-012-0280-z
更新日期:2013-04-01 00:00:00
abstract::The infant rat model is widely used to study the pathogenesis of meningitis caused by a variety of gram-positive and gram-negative bacteria. However, the interpretation of published results concerning meningitis is difficult in many records because the fact that blood contamination of the cerebrospinal fluid (CSF) can...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s004300050011
更新日期:1996-05-01 00:00:00
abstract::Different culture conditions, devitalizing treatments, and preservation procedures were tested for the production of protein A-bearing cells of Staphylococcus aureus ATCC 12598. Native cells with the highest IgG-binding activity were obtained after cultivation at 37 degrees C for 24-48 h in enriched media. Devitalizat...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02123683
更新日期:1986-01-01 00:00:00
abstract::Various strains of Helicobacter pylori were able to lyse erythrocytes from sheep, horse, and human when grown on blood agar. The hemolysis did not depend on the production of the vacuolating cytotoxin VacA as demonstrated by the hemolytic behavior of an isogenic vacA-negative mutant strain. The hemolytic activity coul...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s004300050062
更新日期:1998-03-01 00:00:00
abstract::Interaction of the nucleocapsid (NP) and V proteins of human parainfluenza type 2 virus (HPIV-2) was investigated using a transient expression system. When the NP proteins were co-expressed with the V proteins, some of the NP proteins were translocated into the nuclei. These findings suggest that the NP protein intera...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s004300050019
更新日期:1996-09-01 00:00:00
abstract::Turkey is seeing a steady rise in rates of HIV infection in the country. The number of individuals with HIV/AIDS was greater than 7000 in 2014 according to data released by the Ministry of Health, and heterosexual contacts were reported to be the main transmission routes. Istanbul has the highest number of reported ca...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-015-0419-9
更新日期:2015-08-01 00:00:00
abstract::EBV-associated polypeptides induced in different Epstein-Barr Virus (EBV)-producing cell lines by the tumor promotor TPA, and from purified EBV particles derived from the same lines were radioactivity labeled and analyzed by immunoprecipitation with human VCA+MA+ sera. In virus-producing cells no significant differenc...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02122703
更新日期:1982-01-01 00:00:00
abstract::The prevalence of enterotoxigenic E coli (ETEC) as a pathogenic agent of diarrhoea in the Tel-Aviv (Israel) area was determined, and the isolated E. coli strains characterized. During three periods (summer 1977, summer 1978, and summer 1979), a total of 335 specimens were tested for the presence of E. coli producing L...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02123712
更新日期:1980-01-01 00:00:00
abstract::Cytomegalovirus vaccine development started in the 1970s with attenuated strains. In the 1980s, one of the strains was shown to be safe and effective in renal transplant patients. Then, attention switched to glycoprotein gB, which was shown to give moderate but transient protection against acquisition of the virus by ...
journal_title:Medical microbiology and immunology
pub_type: 历史文章,杂志文章,评审
doi:10.1007/s00430-015-0388-z
更新日期:2015-06-01 00:00:00
abstract::A coupled luminescent method (CLM) based on glyceraldehyde-3-phosphate dehydrogenase released from injured target cells was used to evaluate the cytotoxicity of antigen-specific HLA class I-restricted CTLs. In contrast to established methods, CLM does not require the pretreatment of target cells with radioactive or to...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-009-0126-5
更新日期:2009-11-01 00:00:00
abstract::Since the dynamics of transmission of human cytomegalovirus (HCMV) have not been clarified yet, we assessed a possible change in HCMV seroprevalence in Frankfurt am Main, Germany during the past twenty years and tried to detect variables with an impact on epidemiology. Between 1/1/1988 and 10/15/2008, a total of 54443...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-009-0136-3
更新日期:2010-02-01 00:00:00
abstract::The yeast Candida albicans is part of the microflora in most healthy people, but can become a pathogen when host defenses are compromised. The phenotypic plasticity of C. albicans, which includes switching between different morphologies, contributes to its ability to colonize and infect virtually all body locations. A...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章,评审
doi:10.1007/s00430-010-0147-0
更新日期:2010-08-01 00:00:00
abstract::BALB/c male mice infected with the H3 variant of coxsackievirus B3 (CVB3) develop fulminant myocarditis. Age-matched female mice show little myocarditis due to decreased virus receptor expression on cardiac cells. TNFalpha and IL-1beta levels were increased in males by 3 days after infection. IFNgamma levels increased...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-004-0221-6
更新日期:2005-05-01 00:00:00
abstract::The pathogenesis of Staphylococcus aureus from local infection to systemic dissemination involves a range of virulence factors including structural and secreted products. Among various control mechanisms, small noncoding RNAs are involved in the regulation of multiple pathogenicity factors in S. aureus. The sRNA SprX ...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-016-0467-9
更新日期:2016-12-01 00:00:00
abstract::IFN-γ release assays (IGRAs) often present false-negative or indeterminate results in children with tuberculosis. HIV co-infection may contribute to decreased sensitivity of IGRAs by impairing T-cell IFN-γ expression. Measurement of alternative cytokines in QuantiFERON® (QFT) supernatants can circumvent the IFN-γ-depe...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-017-0501-6
更新日期:2017-06-01 00:00:00
abstract::Several lines of evidence suggest a viral infection as the initiating event for the development of myocarditis (MC). Especially enteroviruses like coxsackie B3 virus have been shown to induce MC in humans and strains of MC-prone mice after an infection. The further course of the disease is, however, determined not onl...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章,评审
doi:10.1007/s00430-003-0217-7
更新日期:2004-05-01 00:00:00