Abstract:
:EBV-associated polypeptides induced in different Epstein-Barr Virus (EBV)-producing cell lines by the tumor promotor TPA, and from purified EBV particles derived from the same lines were radioactivity labeled and analyzed by immunoprecipitation with human VCA+MA+ sera. In virus-producing cells no significant differences in the molecular weight of 35S-methionine-labeled EBV-associated polypeptide patterns could be observed. The analysis 125I-labeled polypeptides from purified virus particles of four different strains revealed that, in addition to common polypeptides, individual EBV strains contain strain-specific high molecular weight glycopolypeptides. These polypeptides, constituting part of the membrane antigen complex, are present in varying amounts. While P3HR-1 virus particles contain a major component of 250 000 and small amounts of 340 000 molecular weight polypeptides, Q IMR-WIL virus particles have more 340 00 than 240 000 molecular weight polypeptides. Furthermore, in B95-8 particles and in particles from an EBV strain isolated from an African green monkey (AGM-EBV) respectively, large amounts of 360 000 and 250 000 polypeptides could be observed. Since these glycopolypeptides carry strain-, subgroup- and group-specific antigenic determinants, also found in virus strains produced in human and marmoset cells, it should be further investigated whether these differences in molecular weight are virus-strain- or cell-specific.
journal_name
Med Microbiol Immunoljournal_title
Medical microbiology and immunologyauthors
Georg-Fries B,Mueller-Lantzsch Ndoi
10.1007/BF02122703subject
Has Abstractpub_date
1982-01-01 00:00:00pages
11-21issue
1eissn
0300-8584issn
1432-1831journal_volume
171pub_type
杂志文章abstract::The production of thermolabile (LT) enterotoxin was compared in a defined medium reported by Staples et al. (SAG medium) for the production of thermostable (ST) enterotoxin and the Casamino acids-Yeast extract (CAYE) medium. Aliquots were drawn frum cultures of an enterotoxigenic (LT+, ST+) E. coli in both media at di...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02122706
更新日期:1982-01-01 00:00:00
abstract::In the present study, synthetic peptides (EENVEHDA)2 [(oc)2] and (DDEHVEEPTVA)2 [(un)2] of ring-infected erythrocyte surface antigen (RESA) of Plasmodlium filciparum were linked with palmitic acid and entrapped in immunostimulating complexes (ISCOMs). The immunogenicity of the peptide(s) and mixture of peptides were s...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s004300000042
更新日期:2000-11-01 00:00:00
abstract::A sentinel surveillance system of the public health service for acute respiratory diseases (ARD), especially influenza, has been realized in the Free State of Saxony of Germany for 25 years. The epidemiological sentinel is based on weekly registration of morbidity and mathematical-statistical calculations. For a micro...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-002-0136-z
更新日期:2002-12-01 00:00:00
abstract::Moraxella catarrhalis is an important pathogen of respiratory and middle ear infections. We previously reported that the attachment of M. catarrhalis to pharyngeal epithelial cells is mediated by ganglioside M2 (GM2). Several sets of adhesins or receptors are involved in such attachment process. In this study, we used...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-002-0109-2
更新日期:2002-05-01 00:00:00
abstract::The ability of polymorphonuclear leucocytes (PMNs) from elderly healthy subjects and post-operative cancer patients to ingest and kill Clostridium difficile was studied. The percentage of phagocytosis in clinically healthy subjects aged 69-82 years ranged from 20.2 +/- 3.2 (mean +/- SD) to 34.1 +/- 4.2, depending on t...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02123569
更新日期:1984-01-01 00:00:00
abstract::A microcytoxicity assay with 3H-proline prelabeled target cells was used for the detection of sensitized lymphoid spleen cells from STU inbred mice inoculated with Moloney sarcoma virus (MSV-M) or ascitic MSV-M tumor cells. The target cell line was derived from ascitic MSV-M tumor cells. With regard to the specificity...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02121319
更新日期:1976-06-01 00:00:00
abstract::Low public awareness of cytomegalovirus (CMV) results from the only mild and transient symptoms that it causes in the healthy immunocompetent host, so that primary infection usually goes unnoticed. The virus is not cleared, however, but stays for the lifetime of the host in a non-infectious, replicatively dormant stat...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章,评审
doi:10.1007/s00430-012-0273-y
更新日期:2012-11-01 00:00:00
abstract::Neutrophil extracellular traps (NETs) have recently been discovered as a central part of antimicrobial innate immunity. In the meanwhile, evidence accumulated that NETs are also generated upon non-infectious stimuli in various clinical settings. In acute or chronic inflammatory disorders aberrantly enhanced NET format...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章,评审
doi:10.1007/s00430-009-0121-x
更新日期:2009-11-01 00:00:00
abstract::The sequencing of Leishmania major Friedlin chromosome 1 (Chr1), Chr3, and Chr4 has been completed. and several other chromosomes are well underway. The complete genome sequence should be available by 2003. Over 1,000 full-length new genes have been identified, with the majority (approximately 75%) having unknown func...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s004300100070
更新日期:2001-11-01 00:00:00
abstract::Phosphorylcholine (ChoP) is covalently incorporated into bacterial surface structures, contributing to host mimicry and promoting adhesion to surfaces. Our aims were to determine the frequency of ChoP display among Aggregatibacter actinomycetemcomitans strains, to clarify which surface structures bear ChoP, and whethe...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-018-0554-1
更新日期:2018-11-01 00:00:00
abstract::Tests were made on 169 sera from children up to 10 years of age for rotavirus antibodies by indirect immunofluorescence and complement fixation tests. The simian strain SA 11 served as a substitute antigen for the human rotavirus. Furthermore, the SA 11 complement-fixing antigen was compared with a commercial antigen ...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02123296
更新日期:1979-03-13 00:00:00
abstract::In a randomized, placebo-controlled trial in Ghana, 67 onchocerciasis patients received 200-mg/day doxycycline for 4-6 weeks, followed by ivermectin (IVM) after 6 months. After 6-27 months, efficacy was evaluated by onchocercoma histology, PCR and microfilariae determination. Administration of doxycycline resulted in ...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00430-007-0062-1
更新日期:2008-09-01 00:00:00
abstract::A method for the purification and concentration of Cytomegalovirus nucleocapsids and particles complete with envelope is described. Antibody to either nucleocapsid or envelope antigens can be detected in human sera. In patients with CMV disease the antibodies to the envelope antigen could not be detected before the 3r...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02121005
更新日期:1975-07-02 00:00:00
abstract::The present study describes the respiratory immune response of mice to locally administered antigen, and the modulation of this response by systemic immunization. Intranasal immunization of mice with the A/PR/8/34 strain of influenza virus evoked local antibody response of the IgA type. The titer of the IgA antibodies...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02121133
更新日期:1978-11-17 00:00:00
abstract::A total of 603 serum samples obtained from 12 different patient and control groups, including potentially cross-reactive sera, were tested for the presence of antibodies against Kaposi's sarcoma-associated herpesvirus (KSHV, HHV-8). The assays used were an inhouse immunofluorescence test (IFT) employing latent KSHV an...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-001-0098-6
更新日期:2001-12-01 00:00:00
abstract::We have shown that the P and V proteins of human parainfluenza virus type 2 (hPIV-2) bind to genomic RNA by using Northwestern blot analysis. To identify the RNA-binding regions on the P and V proteins, we used a set of deletion mutants produced in Escherichia coli. One region required for the RNA-binding was found in...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-005-0244-7
更新日期:2006-03-01 00:00:00
abstract::Oral candidiasis (OC) is a frequent oral manifestation of HIV infection, is a marker disease and occurs as a pseudomembranous, erythematous or rarely hyperplastic variant; angular cheilitis is also seen. Candida albicans is frequently isolated but other species such as C. krusei and C. dublienensis are emerging. Resis...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章,评审
doi:10.1007/s00430-002-0175-5
更新日期:2003-08-01 00:00:00
abstract::Both humoral and cell-mediated immune responses were studied in reactivated toxoplasma infection in mice. The infection was established by immunosuppressing chronically infected animals by thymectomy followed by irradiation. The reactivated infection was confirmed by reappearance of toxoplasma tachyzoites in peritonea...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF00191061
更新日期:1989-01-01 00:00:00
abstract::Immunodominant proteins are variable in molecular and antigenic structure among different genospecies of Borrelia burgdorferi sensu lato. We have recently developed an immunoblot using five recombinant antigens: the chromosomal-encoded B. burgdorferi proteins p100, the flagellin and an internal flagellin fragment ther...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF00193630
更新日期:1994-02-01 00:00:00
abstract::The Enders strain of mumps virus grown in ovo was purified by differential and equilibrium sucrose gradient sedimentation. Purified virus contained seven polypeptides of mol. wts 68,000, 66,000, 61,000, 54,000, 52,000, 49,000, 47,000. Nucleocapsids isolated from DOC-treated virus contained two polypeptides of mol. wts...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02125509
更新日期:1977-12-27 00:00:00
abstract::IFN-γ release assays (IGRAs) often present false-negative or indeterminate results in children with tuberculosis. HIV co-infection may contribute to decreased sensitivity of IGRAs by impairing T-cell IFN-γ expression. Measurement of alternative cytokines in QuantiFERON® (QFT) supernatants can circumvent the IFN-γ-depe...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-017-0501-6
更新日期:2017-06-01 00:00:00
abstract::Proteus mirabilis (P. mirabilis), a gram-negative enteric bacterium, frequently causes urinary tract infections. Many virulence factors of uropathogenic P. mirabilis have been identified, including urease, flagella, hemolysin and fimbriae. However, the functions of polyphosphate kinase (PPK), which are related to the ...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-015-0430-1
更新日期:2016-04-01 00:00:00
abstract::Chronic hepatitis C virus (HCV) infections affect 71 million people worldwide, often resulting in severe liver damage. Since 2014 highly efficient therapies based on directly acting antivirals (DAAs) are available, offering cure rates of almost 100%, if the infection is diagnosed in time. It took more than a decade to...
journal_title:Medical microbiology and immunology
pub_type: 历史文章,杂志文章,评审
doi:10.1007/s00430-018-0566-x
更新日期:2019-02-01 00:00:00
abstract::In resource-limited or point-of-care settings, rapid diagnostic tests (RDTs), that aim to simultaneously detect HIV antibodies and p24 capsid (p24CA) antigen with high sensitivity, can pose important alternatives to screen for early infections. We evaluated the performance of the antibody and antigen components of the...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-019-00655-0
更新日期:2020-04-01 00:00:00
abstract::Fourteen-day-old cultures of dissociated newborn mouse brain cells were infected separately with different strains of vaccinia virus and a strain of measles virus. Using the indirect immunofluorescence technique we found that under the experimental conditions in these cultures both measles and the neurotropic strain o...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02298200
更新日期:1982-01-01 00:00:00
abstract::Escherichia coli isolates of serotype O6:K5 are the most common causative agents of cystitis and pyelonephritis in adults. To answer the question, as to whether strains of this particular serotype represent one special clonal group, out of a collection of 34 serotype O6:K5 isolates [Zingler et al. (1990) Zentralbl. Ba...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF00195947
更新日期:1993-03-01 00:00:00
abstract::We compared the impact of IL-4 and IL-5 deficiency during the fully permissive infection of BALB/c mice with the rodent filaria Litomosoides sigmodontis. IL-5, in contrast to IL-4, is crucial for the containment of adult worms during short- and long-term infections. IL-5 KO mice allowed development of more larvae into...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-002-0155-9
更新日期:2003-02-01 00:00:00
abstract::The stimulatory effect of lipopolysaccharide (LPS) on human macrophages was found to be neutralized by the detergent octylglucoside (OG). Both macrophage stimulation and reactivity in a limulus amebocyte lysate test were suppressed by suspension of LPS in OG at concentrations between 0.25 and 2.5 mM, whereas other sti...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s430-001-8019-2
更新日期:2001-04-01 00:00:00
abstract::Convulsions and encephalopathy are common complications of Shiga toxin (Stx)-producing Shigella and enterohemorrhagic Escherichia coli infections. In previous studies, we demonstrated that Stx and lipopolysaccharide (LPS) act in concert to enhance mice sensitivity to pentylenetetrazole (PTZ)-induced seizures via mecha...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-001-0108-8
更新日期:2002-03-01 00:00:00
abstract::The prophylactic intranasal medication with a new antiviral compound, 1-phenyl-3-(4 phenyl-2-thiazolyl) guanidine (CL 88,277) was evaluated in humans against rhinovirus 44 challenge. One ml containing 250 mg of CL 88,277 was administered to 10 seronegative volunteers three times a day plus one dose prior to the rhinov...
journal_title:Medical microbiology and immunology
pub_type: 临床试验,杂志文章
doi:10.1007/BF02126707
更新日期:1977-05-18 00:00:00