Abstract:
:Antibodies against the human coronavirus OC43 in human sera were measured by haemagglutination inhibition (HI), complement fixation (CF), and radial diffusion-haemolysis in gel (HIG) techniques. The apparent HI titres in a fraction of sera with no antibodies detectable by the two other methods were found to be reduced considerably after treating the sera with phospholipase C (PLC). The PLC treatment also reduced the apparent HI titres in some sera containing variable amounts of CF and/or HIG antibodies, but did not affect antibody determinations by the two latter methods. These results suggest that false positive results can be obtained in the OC43 HI test unless the sera are treated with phospholipase C before the assay.
journal_name
Med Microbiol Immunoljournal_title
Medical microbiology and immunologyauthors
Hovi Tdoi
10.1007/BF02121147subject
Has Abstractpub_date
1978-11-17 00:00:00pages
173-6issue
1-4eissn
0300-8584issn
1432-1831journal_volume
166pub_type
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