Hepatitis C virus cell culture models: an encomium on basic research paving the road to therapy development.

abstract：:As enteroviruses are mainly transmitted by the fecal-oral route, this study was initiated to investigate the nature of the binding of enteroviruses to human skin. Using poliovirus 1, Mahoney, we investigated the overall effectiveness of soap and water hand-washing of 1 and 5 min duration. The virus-skin interaction wa...

journal_title：Medical microbiology and immunology

authors： Schürmann W,Eggers HJ

更新日期：1985-01-01 00:00:00

abstract：:The pathogenesis of Staphylococcus aureus from local infection to systemic dissemination involves a range of virulence factors including structural and secreted products. Among various control mechanisms, small noncoding RNAs are involved in the regulation of multiple pathogenicity factors in S. aureus. The sRNA SprX ...

journal_title：Medical microbiology and immunology

authors： Kathirvel M,Buchad H,Nair M

更新日期：2016-12-01 00:00:00

abstract：:Oral candidiasis (OC) is a frequent oral manifestation of HIV infection, is a marker disease and occurs as a pseudomembranous, erythematous or rarely hyperplastic variant; angular cheilitis is also seen. Candida albicans is frequently isolated but other species such as C. krusei and C. dublienensis are emerging. Resis...

journal_title：Medical microbiology and immunology

authors： Reichart PA

更新日期：2003-08-01 00:00:00

abstract：:A new way of measuring indirect immunofluorescence (IIF) of microscopic bacterial slide preparations by videocamera and an image processing system is presented. This method is compared with the conventional method of reading the slides by eye. The advantages of this new approach are objective reading, greater accuracy...

journal_title：Medical microbiology and immunology

authors： Apperloo-Renkema HZ,Wilkinson MH,Oenema DG,van der Waaij D

更新日期：1991-01-01 00:00:00

abstract：:In this study, we presented the construction of a library of expression clones for the herpes simplex virus type 1 (HSV-1) proteome and subcellular localization map of HSV-1 proteins in living cells using yellow fluorescent protein (YFP) fusion proteins. As a result, 21 proteins showed cytoplasmic or subcytoplasmic lo...

journal_title：Medical microbiology and immunology

authors： Xing J,Wang S,Li Y,Guo H,Zhao L,Pan W,Lin F,Zhu H,Wang L,Li M,Wang L,Zheng C

更新日期：2011-02-01 00:00:00

abstract：:The ability of polymorphonuclear leucocytes (PMNs) from elderly healthy subjects and post-operative cancer patients to ingest and kill Clostridium difficile was studied. The percentage of phagocytosis in clinically healthy subjects aged 69-82 years ranged from 20.2 +/- 3.2 (mean +/- SD) to 34.1 +/- 4.2, depending on t...

journal_title：Medical microbiology and immunology

authors： Bassaris HP,Lianou PE,Legakis NJ,Papavassiliou JT

更新日期：1984-01-01 00:00:00

abstract：:Presence of point mutations within the drug resistance determining regions of Mycobacterium leprae (M. leprae) genome confers molecular basis of drug resistance to dapsone, rifampin and ofloxacin in leprosy. This study is focused on the identification of mutations within the rpoB gene region of M. leprae that are spec...

journal_title：Medical microbiology and immunology

authors： Vedithi SC,Lavania M,Kumar M,Kaur P,Turankar RP,Singh I,Nigam A,Sengupta U

更新日期：2015-04-01 00:00:00

abstract：:The endogenous danger signal bradykinin was recently found implicated in the development of immunity against parasites via dendritic cells. We here report an essential role of the B(2) (B(2)R) bradykinin receptor in the early immune response against Listeria infection. Mice deficient in B(2)R (B(2)R(-/-) mice) were sh...

journal_title：Medical microbiology and immunology

authors： Kaman WE,Wolterink AF,Bader M,Boele LC,van der Kleij D

更新日期：2009-02-01 00:00:00

abstract：:Surface proteins of nine Campylobacter jejuni strains belonging to three different serovia were extracted with lysozyme/ethylenediamine-tetraacetic acid. The preparations bound to isolated murine small intestinal cells and to a membrane fraction (MF) of isolated brush borders obtained by detergent treatment with Trito...

journal_title：Medical microbiology and immunology

authors： Moser I,Hellmann E

更新日期：1989-01-01 00:00:00

abstract：:Influenza remains an important threat for human health, despite the extensive study of influenza viruses and the production of effective vaccines. In contrast to virus genetics determinants, host genetic factors with clinical impact remained unexplored until recently. The association between three single nucleotide po...

journal_title：Medical microbiology and immunology

authors： Chatzopoulou F,Gioula G,Kioumis I,Chatzidimitriou D,Exindari M

更新日期：2019-10-01 00:00:00

abstract：:A microcytoxicity assay with 3H-proline prelabeled target cells was used for the detection of sensitized lymphoid spleen cells from STU inbred mice inoculated with Moloney sarcoma virus (MSV-M) or ascitic MSV-M tumor cells. The target cell line was derived from ascitic MSV-M tumor cells. With regard to the specificity...

journal_title：Medical microbiology and immunology

authors： Weiland E,Mussgay M

更新日期：1976-06-01 00:00:00

abstract：:IFN-γ release assays (IGRAs) often present false-negative or indeterminate results in children with tuberculosis. HIV co-infection may contribute to decreased sensitivity of IGRAs by impairing T-cell IFN-γ expression. Measurement of alternative cytokines in QuantiFERON® (QFT) supernatants can circumvent the IFN-γ-depe...

journal_title：Medical microbiology and immunology

authors： Lundtoft C,Awuah AA,Nausch N,Enimil A,Mayatepek E,Owusu-Dabo E,Jacobsen M

更新日期：2017-06-01 00:00:00

abstract：:Immediate-early (IE) genes are the first genes to be transcribed during the lytic replication cycle of cytomegaloviruses (CMV), and encode nonstructural proteins, which are assumed to have mainly regulatory functions. The IE proteins may play important roles in the pathogenesis of CMV in vivo, for instance during the ...

journal_title：Medical microbiology and immunology

authors： Busche A,Angulo A,Kay-Jackson P,Ghazal P,Messerle M

更新日期：2008-06-01 00:00:00

abstract：:Varicella during pregnancy can be associated with severe illnesses for both the mother and her neonate. Varicella pneumonia must be regarded as a medical emergency, since pregnant women are at risk of life-threatening ventilatory compromise and death. After maternal chickenpox in the first and second trimesters, conge...

journal_title：Medical microbiology and immunology

authors： Sauerbrei A,Wutzler P

更新日期：2007-06-01 00:00:00

abstract：:Tetraspanin (TSPAN) protein family forms a family of transmembrane proteins that act as organizers/scaffold for other proteins. TSPANs are primarily present on plasma membranes although they are also found in other biological membranes. They are organized in tetraspanin-enriched microdomains (TEMs), which allow spatio...

journal_title：Medical microbiology and immunology

authors： Perot BP,Ménager MM

更新日期：2020-08-01 00:00:00

abstract：:A 33-kDa protein (TB33) was isolated from a delipidated cell sonicate (CS) of Mycobacterium tuberculosis H37Rv (grown in Middlebrook 7H9 broth supplemented with glucose) using immobilized metal affinity chromatography (IMAC) on a nickel-nitrilotriacetic acid (Ni-NTA) column. TB33 could not be isolated from the culture...

journal_title：Medical microbiology and immunology

authors： Deshpande RG,Khan MB,Bhat DA,Navalkar RG

更新日期：1996-11-01 00:00:00

abstract：:9 antigen distribution and sensitivity to the bactericidal effect of normal human serum were determined in 485 Proteus strains isolated from urine, blood, wounds, and feces. Of all strains, 62% could be grouped according to 25 0 antigens. The most frequent one, 0 3, appeared in 13% of all the strains. In blood culture...

journal_title：Medical microbiology and immunology

authors： Larsson P,Olling S

更新日期：1977-07-18 00:00:00

abstract：:A skim-milk fraction and a whey-protein concentrate (WPC) fraction were prepared from the cows that had been immunized with E. coli isolated from the mouse intestine. The antibacterial effect of these fractions against E. coli was examined. They contained antibody with a high affinity for E. coli strain 48, a represen...

journal_title：Medical microbiology and immunology

authors： Nomoto K,Matsuoka Y,Hayakawa K,Ohwaki M,Kan T,Yoshikai Y,Nomoto K

更新日期：1992-01-01 00:00:00

abstract：:We assessed whether the intracellular bacterium Chlamydia pneumoniae was present in post-mortem brain samples from patients with and without late-onset Alzheimer's disease (AD), since some indirect evidence seems to suggest that infection with the organism might be associated with the disease. Nucleic acids prepared f...

journal_title：Medical microbiology and immunology

authors： Balin BJ,Gérard HC,Arking EJ,Appelt DM,Branigan PJ,Abrams JT,Whittum-Hudson JA,Hudson AP

更新日期：1998-06-01 00:00:00

abstract：:The sequencing of Leishmania major Friedlin chromosome 1 (Chr1), Chr3, and Chr4 has been completed. and several other chromosomes are well underway. The complete genome sequence should be available by 2003. Over 1,000 full-length new genes have been identified, with the majority (approximately 75%) having unknown func...

journal_title：Medical microbiology and immunology

authors： Myler PJ,Beverley SM,Cruz AK,Dobson DE,Ivens AC,McDonagh PD,Madhubala R,Martinez-Calvillo S,Ruiz JC,Saxena A,Sisk E,Sunkin SM,Worthey E,Yan S,Stuart KD

更新日期：2001-11-01 00:00:00

abstract：:Various strains of Helicobacter pylori were able to lyse erythrocytes from sheep, horse, and human when grown on blood agar. The hemolysis did not depend on the production of the vacuolating cytotoxin VacA as demonstrated by the hemolytic behavior of an isogenic vacA-negative mutant strain. The hemolytic activity coul...

journal_title：Medical microbiology and immunology

authors： Bereswill S,Fassbinder F,Völzing C,Covacci A,Haas R,Kist M

更新日期：1998-03-01 00:00:00

abstract：:The effects of sheep anti-murine recombinant tumor necrosis factor-alpha (TNF-alpha) on resistance to Listeria monocytogenes infection were studied in T cell-deficient nu/nu mice. The sheep anti-TNF-alpha antibody preparation was specific for TNF since it neutralized 300 U of recombinant murine TNF-alpha in vitro at a...

journal_title：Medical microbiology and immunology

authors： Hauser T,Frei K,Zinkernagel RM,Leist TP

更新日期：1990-01-01 00:00:00

abstract：:This is a summary of the recent work on some glycosidases of sand flies and their Leishmania parasites. Glycosidases catalyze the hydrolysis of complex sugar subunits of polysaccharides into simple sugars. Leishmania major parasites secrete chitinase and N-acetylglucosaminase, which enables them to survive in the gut ...

journal_title：Medical microbiology and immunology

authors： Jacobson RL,Schlein Y,Eisenberger CL

更新日期：2001-11-01 00:00:00

abstract：:The antiviral activity of mouse fibroblast interferon against vesicular stomatitis virus was investigated in L-929 mouse fibroblasts and the ganglioside-deficient L-929 mutant cells (ATCC clone NCTC 2071). Although it has been widely reported that gangliosides serve as primary receptors for interferon at the cellular ...

journal_title：Medical microbiology and immunology

authors： Schiffmann D,Koschel K

更新日期：1981-01-01 00:00:00

abstract：:Both aging and treated human immunodeficiency virus (HIV) infection are characterized by low-level chronic inflammation and immune activation which contribute to the development of age-related diseases, frailty, and early mortality. Chronic cytomegalovirus (CMV) infection is highly prevalent in older adults and HIV-in...

journal_title：Medical microbiology and immunology

authors： Tavenier J,Margolick JB,Leng SX

更新日期：2019-08-01 00:00:00

abstract：:Industrial and academic needs for innovation and fundamental research are essential and not widely different. Depending on the industrial setting, research and development (R&D) activities may be more focused on the developmental aspects given the need to ultimately sell useful products. However, one of the biggest di...

journal_title：Medical microbiology and immunology

authors： van Belkum A,Pelegrin AC,Datar R,Goyal M,Palmieri M,Mirande C,Chalansonnet V,Orenga S

更新日期：2020-06-01 00:00:00

abstract：:The propagation of hepatitis A virus (HAV) is shown in a rapidly growing fetal rhesus monkey kidney cell line (Frhk-4/R). In the course of ten passages through Frhk-4/R cells the HAV, which was released in the supernatants of the cell cultures, was adapted to these cells. In the tenth passage 6 days after infection, i...

journal_title：Medical microbiology and immunology

authors： Flehmig B

更新日期：1981-01-01 00:00:00

abstract：:To characterize the role of specific lymphocyte subsets in Chlamydia trachomatis infection, we established a murine model using the mouse pneumonitis agent (MoPn) of C. trachomatis and C.B-17 scid/scid (SCID) mice which lack functional B and T cells. After intraperitoneal inoculation with the bacteria, SCID mice devel...

journal_title：Medical microbiology and immunology

authors： Thoma-Uszynski S,Simnacher U,Marre R,Essig A

更新日期：1998-10-01 00:00:00

abstract：:We compared the impact of IL-4 and IL-5 deficiency during the fully permissive infection of BALB/c mice with the rodent filaria Litomosoides sigmodontis. IL-5, in contrast to IL-4, is crucial for the containment of adult worms during short- and long-term infections. IL-5 KO mice allowed development of more larvae into...

journal_title：Medical microbiology and immunology

authors： Volkmann L,Bain O,Saeftel M,Specht S,Fischer K,Brombacher F,Matthaei KI,Hoerauf A

更新日期：2003-02-01 00:00:00

abstract：:Human cytomegalovirus (HCMV) is the most common virus infection in the developing fetus. A fraction of infants infected in utero develop significant life-threatening and organ-threatening disease with over 90% of infected infants exhibiting no clinical evidence of infection in the newborn period. However, about 10% of...

journal_title：Medical microbiology and immunology

authors： Britt W

更新日期：2015-06-01 00:00:00