Objective quantitation of serum antibody titres against Enterobacteriaceae using indirect immunofluorescence, read by videocamera and image processing system.

Abstract:

:A new way of measuring indirect immunofluorescence (IIF) of microscopic bacterial slide preparations by videocamera and an image processing system is presented. This method is compared with the conventional method of reading the slides by eye. The advantages of this new approach are objective reading, greater accuracy and easier performance. We have applied the method to measure serum antibody titres against endogenous Enterobacteriaceae. The method offers the opportunity to combine IIF with automatic morphological analysis, thereby maximally exploiting the possibilities of the immunofluorescence technique.

journal_name

Med Microbiol Immunol

authors

Apperloo-Renkema HZ,Wilkinson MH,Oenema DG,van der Waaij D

doi

10.1007/BF00193850

subject

Has Abstract

pub_date

1991-01-01 00:00:00

pages

93-100

issue

2

eissn

0300-8584

issn

1432-1831

journal_volume

180

pub_type

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