Abstract:
:IgG immune response to CagA was evaluated by enzyme-linked imunosorbent assay (ELISA) using a recombinant fragment of CagA as antigen in 171 patients with gastric cancer and 298 blood donors to determine whether it could be related to the ABO(H) blood group phenotype, stage of cancer or tumor morphology. The CagA-ELISA showed a good specificity (93.5%) and sensitivity (88.5%) as compared with immunoblotting for blot CagA-negative and -positive donors. The Helicobacter pylori seropositive blood group A donors revealed the lowest proportion (37.6%) of strong responders to CagA: A journal_name journal_title authors doi keywords: subject pub_date pages issue eissn issn journal_volume pub_type
abstract::A total of 603 serum samples obtained from 12 different patient and control groups, including potentially cross-reactive sera, were tested for the presence of antibodies against Kaposi's sarcoma-associated herpesvirus (KSHV, HHV-8). The assays used were an inhouse immunofluorescence test (IFT) employing latent KSHV an...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-001-0098-6
更新日期:2001-12-01 00:00:00
abstract::Antibodies against the human coronavirus OC43 in human sera were measured by haemagglutination inhibition (HI), complement fixation (CF), and radial diffusion-haemolysis in gel (HIG) techniques. The apparent HI titres in a fraction of sera with no antibodies detectable by the two other methods were found to be reduced...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02121147
更新日期:1978-11-17 00:00:00
abstract::Illness and death of mice infected by intracerebral inoculation with lymphocytic choriomeningitis virus are pathologic immune phenomena, and mice thus infected were protected when immunosuppressed with cyclosporin A (CSA), although the virus multiplied extensively in all major organs. Concentrations remained high as l...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02123695
更新日期:1985-01-01 00:00:00
abstract::Carbapenems are important last-line antibiotics for the treatment of hospital infections. Enterobacteriaceae (such as Klebsiella pneumoniae or Escherichia coli) expressing the "New Delhi Metallo-β-lactamase" gene bla(NDM-1) are resistant to carbapenems and were predicted to become a major global health problem. To cop...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-011-0189-y
更新日期:2011-05-01 00:00:00
abstract::Homeostasis underpins at a systems level the regulatory control of immunity and metabolism. While physiologically these systems are often viewed as independent, there is increasing evidence showing a tight coupling between immune and metabolic functions. Critically upon infection, the homeostatic regulation for both i...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章,评审
doi:10.1007/s00430-015-0402-5
更新日期:2015-06-01 00:00:00
abstract::Industrial and academic needs for innovation and fundamental research are essential and not widely different. Depending on the industrial setting, research and development (R&D) activities may be more focused on the developmental aspects given the need to ultimately sell useful products. However, one of the biggest di...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章,评审
doi:10.1007/s00430-019-00643-4
更新日期:2020-06-01 00:00:00
abstract::The yeast Candida albicans is part of the microflora in most healthy people, but can become a pathogen when host defenses are compromised. The phenotypic plasticity of C. albicans, which includes switching between different morphologies, contributes to its ability to colonize and infect virtually all body locations. A...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章,评审
doi:10.1007/s00430-010-0147-0
更新日期:2010-08-01 00:00:00
abstract::A sentinel surveillance system of the public health service for acute respiratory diseases (ARD), especially influenza, has been realized in the Free State of Saxony of Germany for 25 years. The epidemiological sentinel is based on weekly registration of morbidity and mathematical-statistical calculations. For a micro...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-002-0136-z
更新日期:2002-12-01 00:00:00
abstract::Presence of point mutations within the drug resistance determining regions of Mycobacterium leprae (M. leprae) genome confers molecular basis of drug resistance to dapsone, rifampin and ofloxacin in leprosy. This study is focused on the identification of mutations within the rpoB gene region of M. leprae that are spec...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-014-0354-1
更新日期:2015-04-01 00:00:00
abstract::Immunodominant proteins are variable in molecular and antigenic structure among different genospecies of Borrelia burgdorferi sensu lato. We have recently developed an immunoblot using five recombinant antigens: the chromosomal-encoded B. burgdorferi proteins p100, the flagellin and an internal flagellin fragment ther...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF00193630
更新日期:1994-02-01 00:00:00
abstract::Interaction of the nucleocapsid (NP) and V proteins of human parainfluenza type 2 virus (HPIV-2) was investigated using a transient expression system. When the NP proteins were co-expressed with the V proteins, some of the NP proteins were translocated into the nuclei. These findings suggest that the NP protein intera...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s004300050019
更新日期:1996-09-01 00:00:00
abstract::Different culture conditions, devitalizing treatments, and preservation procedures were tested for the production of protein A-bearing cells of Staphylococcus aureus ATCC 12598. Native cells with the highest IgG-binding activity were obtained after cultivation at 37 degrees C for 24-48 h in enriched media. Devitalizat...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02123683
更新日期:1986-01-01 00:00:00
abstract::It is known that tetraspanin proteins are involved in many physiological somatic cell mechanisms. Additionally, research has indicated they also have a role in various infectious diseases and cancers. This review focuses on the molecular interactions underlying the tetraspanin web formation in gametes. Primarily, tetr...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章,评审
doi:10.1007/s00430-020-00676-0
更新日期:2020-08-01 00:00:00
abstract::Outer membrane fractions (OMs) of nine Campylobacter (C.) jejuni and two C. coli strains belonging to different serovars, from human and various animal origins, were extracted by treatment with sodium N-lauryl sarcosinate. Using n-octyl-beta-D-glucopyranoside a 42-kDa protein and a flagella-enriched fraction were obta...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF00191550
更新日期:1992-01-01 00:00:00
abstract::Monoclonal antibody (MAb) 2c, specific for glycoprotein B of herpes simplex virus (HSV), had been shown to mediate clearance of infection from the mucous membranes of mice, thereby completely inhibiting mucocutaneous inflammation and lethality, even in mice depleted of both CD4(+) and CD8(+) cells. Additionally, gangl...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-010-0174-x
更新日期:2011-05-01 00:00:00
abstract::A coupled luminescent method (CLM) based on glyceraldehyde-3-phosphate dehydrogenase released from injured target cells was used to evaluate the cytotoxicity of antigen-specific HLA class I-restricted CTLs. In contrast to established methods, CLM does not require the pretreatment of target cells with radioactive or to...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-009-0126-5
更新日期:2009-11-01 00:00:00
abstract::The ability of polymorphonuclear leucocytes (PMNs) from elderly healthy subjects and post-operative cancer patients to ingest and kill Clostridium difficile was studied. The percentage of phagocytosis in clinically healthy subjects aged 69-82 years ranged from 20.2 +/- 3.2 (mean +/- SD) to 34.1 +/- 4.2, depending on t...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02123569
更新日期:1984-01-01 00:00:00
abstract::Pseudomonas aeruginosa is an opportunistic pathogen that utilizes the quorum-sensing (QS) process to regulate the production of different virulence factors and biofilm. N-3-oxo-dodecanoyl-L-homoserine lactone (C12) is a key QS molecule of P. aeruginosa which interacts with the mammalian immune cells and modulates thei...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-019-00631-8
更新日期:2019-12-01 00:00:00
abstract::The rapid and correct identification of pathogens is of paramount importance for the treatment of patients with invasive infections. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) can speed up the identification of bacteria and fungi and has quickly been embraced by medical...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-013-0319-9
更新日期:2014-04-01 00:00:00
abstract::We have shown that the P and V proteins of human parainfluenza virus type 2 (hPIV-2) bind to genomic RNA by using Northwestern blot analysis. To identify the RNA-binding regions on the P and V proteins, we used a set of deletion mutants produced in Escherichia coli. One region required for the RNA-binding was found in...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-005-0244-7
更新日期:2006-03-01 00:00:00
abstract::BALB/c male mice infected with the H3 variant of coxsackievirus B3 (CVB3) develop fulminant myocarditis. Age-matched female mice show little myocarditis due to decreased virus receptor expression on cardiac cells. TNFalpha and IL-1beta levels were increased in males by 3 days after infection. IFNgamma levels increased...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-004-0221-6
更新日期:2005-05-01 00:00:00
abstract::The production of thermolabile (LT) enterotoxin was compared in a defined medium reported by Staples et al. (SAG medium) for the production of thermostable (ST) enterotoxin and the Casamino acids-Yeast extract (CAYE) medium. Aliquots were drawn frum cultures of an enterotoxigenic (LT+, ST+) E. coli in both media at di...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02122706
更新日期:1982-01-01 00:00:00
abstract::Chronic hepatitis C virus (HCV) infections affect 71 million people worldwide, often resulting in severe liver damage. Since 2014 highly efficient therapies based on directly acting antivirals (DAAs) are available, offering cure rates of almost 100%, if the infection is diagnosed in time. It took more than a decade to...
journal_title:Medical microbiology and immunology
pub_type: 历史文章,杂志文章,评审
doi:10.1007/s00430-018-0566-x
更新日期:2019-02-01 00:00:00
abstract::The present study analyses the susceptibility of human bladder-derived cells (HT-1376) to the infection by herpes simplex virus type 2 (HSV-2) and Chlamydia trachomatis, as well as to the adhesiveness of uropathogenic bacteria. HT-1376 cells were efficiently infected by HSV-2 strain 333, as demonstrated by immunofluor...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s004300100067
更新日期:2001-09-01 00:00:00
abstract::Almost 4000 sera from seven African states were examined for antibodies to ATLV/HTLV-1. Between 1% and 8% of healthy people from sub-Saharan Africa have such antibodies. The highest frequency was observed in Gabon. There were considerable variations between villages. The percentage of seropositives and the mean titre ...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02123765
更新日期:1984-01-01 00:00:00
abstract::Gliosis of glial fibrillary acidic protein (GFAP) associated astrocytes is considered to be one of the hallmarks of transmissible spongiform encephalopathies (TSEs). In the present study, remarkable GFAP-PrP(Sc) or GFAP-PrP(C) complexes were separately detected in the brain homogenates of 263 K (Scrapie)-infected or n...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-007-0071-0
更新日期:2008-12-01 00:00:00
abstract::Tests were made on 169 sera from children up to 10 years of age for rotavirus antibodies by indirect immunofluorescence and complement fixation tests. The simian strain SA 11 served as a substitute antigen for the human rotavirus. Furthermore, the SA 11 complement-fixing antigen was compared with a commercial antigen ...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02123296
更新日期:1979-03-13 00:00:00
abstract::Lack of virus-specific cell-mediated immunity (CMI) is associated with worse viral infection outcome in hematopoietic stem cell transplantation (HSCT). We aimed to evaluate the role of immunological monitoring of Epstein-Barr virus (EBV) infection in addition to virological one in 33 adult and 18 pediatric allogeneic ...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-019-00629-2
更新日期:2019-12-01 00:00:00
abstract::The antiviral activity of mouse fibroblast interferon against vesicular stomatitis virus was investigated in L-929 mouse fibroblasts and the ganglioside-deficient L-929 mutant cells (ATCC clone NCTC 2071). Although it has been widely reported that gangliosides serve as primary receptors for interferon at the cellular ...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02125527
更新日期:1981-01-01 00:00:00
abstract::The administration of drugs to inhibit metabolic pathways not only reduces the risk of obesity-induced diseases in humans but may also hamper the replication of different viral pathogens. In order to investigate the value of the US Food and Drug Administration-approved anti-obesity drug orlistat in view of its anti-vi...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-015-0391-4
更新日期:2015-12-01 00:00:00