Abstract:
:The administration of drugs to inhibit metabolic pathways not only reduces the risk of obesity-induced diseases in humans but may also hamper the replication of different viral pathogens. In order to investigate the value of the US Food and Drug Administration-approved anti-obesity drug orlistat in view of its anti-viral activity against different human-pathogenic viruses, several anti-viral studies, electron microscopy analyses as well as fatty acid uptake experiments were performed. The results indicate that administrations of non-cytotoxic concentrations of orlistat reduced the replication of coxsackievirus B3 (CVB3) in different cell types significantly. Moreover, orlistat revealed cell protective effects and modified the formation of multi-layered structures in CVB3-infected cells, which are necessary for viral replication. Lowering fatty acid uptake from the extracellular environment by phloretin administrations had only marginal impact on CVB3 replication. Finally, orlistat reduced also the replication of varicella-zoster virus moderately but had no significant influence on the replication of influenza A viruses. The data support further experiments into the value of orlistat as an inhibitor of the fatty acid synthase to develop new anti-viral compounds, which are based on the modulation of cellular metabolic pathways.
journal_name
Med Microbiol Immunoljournal_title
Medical microbiology and immunologyauthors
Ammer E,Nietzsche S,Rien C,Kühnl A,Mader T,Heller R,Sauerbrei A,Henke Adoi
10.1007/s00430-015-0391-4subject
Has Abstractpub_date
2015-12-01 00:00:00pages
635-45issue
6eissn
0300-8584issn
1432-1831pii
10.1007/s00430-015-0391-4journal_volume
204pub_type
杂志文章abstract::While the antigen structure of yeast cell walls is well known, the immunological cross reactivity of these is often difficult to interpret. The cross reactions of eight yeasts were tested in rabbit hyperimmune sera by the indirect immunofluorescence test. Among the eight species examined were the medically important y...
journal_title:Medical microbiology and immunology
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journal_title:Medical microbiology and immunology
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