Evaluation of a defined medium for the production of both thermolabile (LT) and thermostable (ST) enterotoxins of Escherichia coli.

abstract：:Escherichia coli isolates of serotype O6:K5 are the most common causative agents of cystitis and pyelonephritis in adults. To answer the question, as to whether strains of this particular serotype represent one special clonal group, out of a collection of 34 serotype O6:K5 isolates [Zingler et al. (1990) Zentralbl. Ba...

journal_title：Medical microbiology and immunology

authors： Zingler G,Blum G,Falkenhagen U,Orskov I,Orskov F,Hacker J,Ott M

更新日期：1993-03-01 00:00:00

abstract：:Varicella during pregnancy can be associated with severe illnesses for both the mother and her neonate. Varicella pneumonia must be regarded as a medical emergency, since pregnant women are at risk of life-threatening ventilatory compromise and death. After maternal chickenpox in the first and second trimesters, conge...

journal_title：Medical microbiology and immunology

authors： Sauerbrei A,Wutzler P

更新日期：2007-06-01 00:00:00

abstract：:The present study analyses the susceptibility of human bladder-derived cells (HT-1376) to the infection by herpes simplex virus type 2 (HSV-2) and Chlamydia trachomatis, as well as to the adhesiveness of uropathogenic bacteria. HT-1376 cells were efficiently infected by HSV-2 strain 333, as demonstrated by immunofluor...

journal_title：Medical microbiology and immunology

authors： Superti F,Longhi C,Di Biase AM,Tinari A,Marchetti M,Pisani S,Gallinelli C,Chiarini F,Seganti L

更新日期：2001-09-01 00:00:00

abstract：:The essential protein lysyl-tRNA synthetase (LysRS) exists in two unrelated forms, a class I and a class II-type aminoacyl-tRNA synthetase. Comparative genome sequence analysis revealed that Borrelia burgdorferi sensu lato, the etiological agent of Lyme disease, contains a class I-type LysRS, whereas its tick and mamm...

journal_title：Medical microbiology and immunology

authors： Mejlhede N,Monthán A,Theisen M,Ibba M

更新日期：2003-05-01 00:00:00

abstract：:We have studied the incidence of enterotoxigenic and enteropathogenic Escherichia coli strains associated with infant diarrhoeal disease in Galicia (North-west Spain). During a period of 9 months we isolated heat-labile enterotoxin-positive strains in 2.1% of children with diarrhoea examined, whereas the production of...

journal_title：Medical microbiology and immunology

authors： Blanco J,González EA,Bernárdez I,Varela BR

更新日期：1983-01-01 00:00:00

abstract：:In order to take the conditions of natural Herpes simplex virus (HSV) infection into consideration, the genetic resistance of C57-Bl mice, which was established by intraperitoneal HSV-1 infection [Lopez, 1975], was investigated in vaginally infected mice. The course of infection in the mucous membranes did not differ ...

journal_title：Medical microbiology and immunology

authors： Schneweis KE,Olbrich M,Saftig V,Scholz R

更新日期：1982-01-01 00:00:00

abstract：:Influenza remains an important threat for human health, despite the extensive study of influenza viruses and the production of effective vaccines. In contrast to virus genetics determinants, host genetic factors with clinical impact remained unexplored until recently. The association between three single nucleotide po...

journal_title：Medical microbiology and immunology

authors： Chatzopoulou F,Gioula G,Kioumis I,Chatzidimitriou D,Exindari M

更新日期：2019-10-01 00:00:00

abstract：:During normal interpandemic influenza seasons, immune responses to vaccines are quite predictable and meet the licensing criteria of the European Union (EU) Committee for Proprietary Medicinal Products (CPMP). In a pandemic situation, large sections, if not all of the community will be immunologically naïve and theref...

journal_title：Medical microbiology and immunology

authors： Wood JM,Nicholson KG,Stephenson I,Zambon M,Newman RW,Major DL,Podda A

更新日期：2002-12-01 00:00:00

abstract：:When P3HR-1 lymphoblastoid cells expressing Epstein-Barr virus (EBV)-related antigens at very low frequency were cocultivated with human umbilical cord blood lymphocytes and the cell-free mixed culture fluid was applied to fresh cord lymphocytes, cells morphologically distinct from normal lymphocytes became evident af...

journal_title：Medical microbiology and immunology

authors： Aya T,Osato T

更新日期：1978-01-01 00:00:00

abstract：:The generation of rodent Plasmodium strains expressing fluorescent proteins in all life cycle stages has had a big impact on malaria research. With this tool in hand, for the first time it was possible to follow in real time by in vivo microscopy the infection route of Plasmodium sporozoites transmitted to the mammali...

journal_title：Medical microbiology and immunology

authors： Sturm A,Heussler V

更新日期：2007-09-01 00:00:00

abstract：:Virus adsorption and uptake of human rhinovirus 14 (HRV14) were studied with HeLa cells and baby hamster kidney (BHK) cells which were transfected with the HRV14 receptor intercellular adhesion molecule-1 (ICAM-1). Transmission electron microscopy of HeLa cells revealed that HRV14 was internalized via clathrin-coated ...

journal_title：Medical microbiology and immunology

authors： Grunert HP,Wolf KU,Langner KD,Sawitzky D,Habermehl KO,Zeichhardt H

更新日期：1997-06-01 00:00:00

abstract：:The antiviral activity of mouse fibroblast interferon against vesicular stomatitis virus was investigated in L-929 mouse fibroblasts and the ganglioside-deficient L-929 mutant cells (ATCC clone NCTC 2071). Although it has been widely reported that gangliosides serve as primary receptors for interferon at the cellular ...

journal_title：Medical microbiology and immunology

authors： Schiffmann D,Koschel K

更新日期：1981-01-01 00:00:00

abstract：:Interaction of the nucleocapsid (NP) and V proteins of human parainfluenza type 2 virus (HPIV-2) was investigated using a transient expression system. When the NP proteins were co-expressed with the V proteins, some of the NP proteins were translocated into the nuclei. These findings suggest that the NP protein intera...

journal_title：Medical microbiology and immunology

authors： Watanabe N,Kawano M,Tsurudome M,Nishio M,Ito M,Ohgimoto S,Suga S,Komada H,Ito Y

更新日期：1996-09-01 00:00:00

abstract：:CAMP factor is an extracellular cytolytic protein produced by Streptococcus agalactiae. CAMP factor has been reported to bind the Fc fragments of immunoglobulin G (IgG) and has therefore also been called protein B, in analogy to protein A of Staphylococcus aureus. We attempted to characterize the interaction of protei...

journal_title：Medical microbiology and immunology

authors： El-Huneidi W,Mui R,Zhang TH,Palmer M

更新日期：2007-06-01 00:00:00

abstract：:The aim of this study was to characterize the cytokine secretion patterns of human T helper cells from healthy donors reactive with somatic antigens from various bacteria, the nematode Anisakis and tetanus toxoid. From the peripheral blood of four healthy donors we have established 70 T cell lines reactive with antige...

journal_title：Medical microbiology and immunology

authors： Schlaak JF,Nieder P,Meyer zum Büschenfelde KH,Fleischer B

更新日期：1994-07-01 00:00:00

abstract：:Epidemiological studies in Mecklenburg-Vorpommern have shown a high prevalence of Borrelia burgdorferi-infected ticks. A total of 17 B. burgdorferi sensu lato strains were isolated from ticks and investigated by Western blots (immunoblot) with eight monoclonal anti-bodies against different epitopes of the outer surfac...

journal_title：Medical microbiology and immunology

authors： Lottmann H,Wilske B,Herrmann H

更新日期：1996-02-01 00:00:00

abstract：:The prevalence of enterotoxigenic E coli (ETEC) as a pathogenic agent of diarrhoea in the Tel-Aviv (Israel) area was determined, and the isolated E. coli strains characterized. During three periods (summer 1977, summer 1978, and summer 1979), a total of 335 specimens were tested for the presence of E. coli producing L...

journal_title：Medical microbiology and immunology

authors： Goldhar J,Peri R,Zilberberg R,Lahav M

更新日期：1980-01-01 00:00:00

abstract：:The pathogenesis of Staphylococcus aureus from local infection to systemic dissemination involves a range of virulence factors including structural and secreted products. Among various control mechanisms, small noncoding RNAs are involved in the regulation of multiple pathogenicity factors in S. aureus. The sRNA SprX ...

journal_title：Medical microbiology and immunology

authors： Kathirvel M,Buchad H,Nair M

更新日期：2016-12-01 00:00:00

abstract：:The streptococcal pyrogenic exotoxin B (SpeB) is known to be involved in group A streptococcus (GAS) survival in blood, but the detailed mechanism is not clear. For clarification of this issue, speB isogenic mutants of strains M6 and M49 were constructed by using an integrational plasmid and confirmed by Southern blot...

journal_title：Medical microbiology and immunology

authors： Chiang-Ni C,Wang CH,Tsai PJ,Chuang WJ,Lin YS,Lin MT,Liu CC,Wu JJ

更新日期：2006-06-01 00:00:00

abstract：:The administration of drugs to inhibit metabolic pathways not only reduces the risk of obesity-induced diseases in humans but may also hamper the replication of different viral pathogens. In order to investigate the value of the US Food and Drug Administration-approved anti-obesity drug orlistat in view of its anti-vi...

journal_title：Medical microbiology and immunology

authors： Ammer E,Nietzsche S,Rien C,Kühnl A,Mader T,Heller R,Sauerbrei A,Henke A

更新日期：2015-12-01 00:00:00

abstract：:The infant rat model is widely used to study the pathogenesis of meningitis caused by a variety of gram-positive and gram-negative bacteria. However, the interpretation of published results concerning meningitis is difficult in many records because the fact that blood contamination of the cerebrospinal fluid (CSF) can...

journal_title：Medical microbiology and immunology

authors： Vogel U,Steinmetz I,Frosch M

更新日期：1996-05-01 00:00:00

abstract：:Pseudomonas aeruginosa is an opportunistic pathogen that utilizes the quorum-sensing (QS) process to regulate the production of different virulence factors and biofilm. N-3-oxo-dodecanoyl-L-homoserine lactone (C12) is a key QS molecule of P. aeruginosa which interacts with the mammalian immune cells and modulates thei...

journal_title：Medical microbiology and immunology

authors： Singh PK,Yadav VK,Kalia M,Sharma D,Pandey D,Agarwal V

更新日期：2019-12-01 00:00:00

abstract：:Thrombi formation was demonstrated in mesenteric microcirculation of guinea pigs inoculated with Fusobacterium necrophorum and the bacterial hemagglutinin (HA). The thrombi were initially observed in venules and later, in arterioles. Immunofluorescence study revealed that the HA bound to the thrombi in the microcircul...

journal_title：Medical microbiology and immunology

authors： Kanoe M,Yamanaka M,Inoue M

更新日期：1989-01-01 00:00:00

abstract：:Neutrophil extracellular traps (NETs) have recently been discovered as a central part of antimicrobial innate immunity. In the meanwhile, evidence accumulated that NETs are also generated upon non-infectious stimuli in various clinical settings. In acute or chronic inflammatory disorders aberrantly enhanced NET format...

journal_title：Medical microbiology and immunology

authors： Lögters T,Margraf S,Altrichter J,Cinatl J,Mitzner S,Windolf J,Scholz M

更新日期：2009-11-01 00:00:00

abstract：:This study describes the properties of an inactivated subunit antigen preparation from varicella-zoster virus (VZV)-infected MRC-5 cells by treatment with detergent and formaldehyde, ultracentrifugation over sucrose and acetone precipitation. The method preserved the antigenicity of VZV proteins and several VZV-specif...

journal_title：Medical microbiology and immunology

authors： Davies J,Hallworth JA,McLeish P,Randall S,Martin BA,Buchan A,Skinner GR

更新日期：1994-05-01 00:00:00

abstract：:9 antigen distribution and sensitivity to the bactericidal effect of normal human serum were determined in 485 Proteus strains isolated from urine, blood, wounds, and feces. Of all strains, 62% could be grouped according to 25 0 antigens. The most frequent one, 0 3, appeared in 13% of all the strains. In blood culture...

journal_title：Medical microbiology and immunology

authors： Larsson P,Olling S

更新日期：1977-07-18 00:00:00

abstract：:Microdilution trays (Sensititre) for antimicrobial susceptibility testing of Bacteroides fragilis has been compared for accuracy with the standard agar dilution method. The microtrays were found to be reliable for all antimicrobials tested (benzylpenicillin, clindamycin, doxycycline, chloramphenicol, fusidic acid, cef...

journal_title：Medical microbiology and immunology

authors： Dornbusch K,Lindmark A,Nord CE

更新日期：1978-10-20 00:00:00

abstract：:Mycobacterium avium subsp. paratuberculosis (MAP) has long been implicated as a triggering agent in Crohn's disease (CD). In this study, we investigated the growth/persistence of both M. avium subsp. hominissuis (MAH) and MAP, in macrophages from healthy controls (HC), CD and ulcerative colitis patients. For viability...

journal_title：Medical microbiology and immunology

authors： Nazareth N,Magro F,Appelberg R,Silva J,Gracio D,Coelho R,Cabral JM,Abreu C,Macedo G,Bull TJ,Sarmento A

更新日期：2015-12-01 00:00:00

abstract：:A coupled luminescent method (CLM) based on glyceraldehyde-3-phosphate dehydrogenase released from injured target cells was used to evaluate the cytotoxicity of antigen-specific HLA class I-restricted CTLs. In contrast to established methods, CLM does not require the pretreatment of target cells with radioactive or to...

journal_title：Medical microbiology and immunology

authors： Ogbomo H,Geiler J,Leutz A,von Kietzell K,Michaelis M,Doerr HW,Cinatl J Jr

更新日期：2009-11-01 00:00:00

abstract：:We have examined 118 oral squamous cell carcinomas, 72 oral leukoplakias, 12 cases of cheilitis and 65 of oral lichen planus for the presence of human papillomavirus (HPV) 6/11, 16 and 18 DNA by PCR/Southern blot hybridization. HPV DNA were found in 51/118 carcinomas (43.2%), in 16/72 (22.2%) leukoplakias, 3/12 (25.0%...

journal_title：Medical microbiology and immunology

authors： Ostwald C,Rutsatz K,Schweder J,Schmidt W,Gundlach K,Barten M

更新日期：2003-08-01 00:00:00