Abstract:
:We have shown that the P and V proteins of human parainfluenza virus type 2 (hPIV-2) bind to genomic RNA by using Northwestern blot analysis. To identify the RNA-binding regions on the P and V proteins, we used a set of deletion mutants produced in Escherichia coli. One region required for the RNA-binding was found in the P-V common domain (aa 1-82). Others were found in the P protein-specific region (aa 249-354) and the V protein-specific region (aa 176-225). In addition, we have shown that substitutions of some basic residues with alanines in these regions abrogate RNA-binding by the P or V proteins. Intriguingly, the P and V proteins of hPIV2 can selectively bind to the viral RNA under our experimental conditions.
journal_name
Med Microbiol Immunoljournal_title
Medical microbiology and immunologyauthors
Nishio M,Tsurudome M,Ito M,Ito Ydoi
10.1007/s00430-005-0244-7keywords:
subject
Has Abstractpub_date
2006-03-01 00:00:00pages
29-36issue
1eissn
0300-8584issn
1432-1831journal_volume
195pub_type
杂志文章abstract::A microcytoxicity assay with 3H-proline prelabeled target cells was used for the detection of sensitized lymphoid spleen cells from STU inbred mice inoculated with Moloney sarcoma virus (MSV-M) or ascitic MSV-M tumor cells. The target cell line was derived from ascitic MSV-M tumor cells. With regard to the specificity...
journal_title:Medical microbiology and immunology
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章
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journal_title:Medical microbiology and immunology
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journal_title:Medical microbiology and immunology
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章
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journal_title:Medical microbiology and immunology
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章,评审
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02126676
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pub_type: 杂志文章
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