Abstract:
:The essential protein lysyl-tRNA synthetase (LysRS) exists in two unrelated forms, a class I and a class II-type aminoacyl-tRNA synthetase. Comparative genome sequence analysis revealed that Borrelia burgdorferi sensu lato, the etiological agent of Lyme disease, contains a class I-type LysRS, whereas its tick and mammalian hosts would be expected to contain a class II-type protein. To investigate the utility of the class I LysRS as a diagnostic target for Lyme disease, the corresponding gene ( lysK) was cloned and sequenced from B. afzelii, B. garinii, and B. hermsii. These lysK sequences were then used to design a primer set that could detect and genotype B. burgdorferisensu strictu, B. afzelii, and B. garinii in one single polymerase chain reaction, while showing no cross reactivity with examples of other Borrelia or spirochetes.
journal_name
Med Microbiol Immunoljournal_title
Medical microbiology and immunologyauthors
Mejlhede N,Monthán A,Theisen M,Ibba Mdoi
10.1007/s00430-002-0149-7keywords:
subject
Has Abstractpub_date
2003-05-01 00:00:00pages
79-83issue
2eissn
0300-8584issn
1432-1831journal_volume
192pub_type
杂志文章abstract::The ability of polymorphonuclear leucocytes (PMNs) from elderly healthy subjects and post-operative cancer patients to ingest and kill Clostridium difficile was studied. The percentage of phagocytosis in clinically healthy subjects aged 69-82 years ranged from 20.2 +/- 3.2 (mean +/- SD) to 34.1 +/- 4.2, depending on t...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02123569
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章
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journal_title:Medical microbiology and immunology
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章
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更新日期:2016-06-01 00:00:00
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章,评审
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更新日期:2015-06-01 00:00:00
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章
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journal_title:Medical microbiology and immunology
pub_type: 临床试验,杂志文章
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章
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更新日期:2000-11-01 00:00:00
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s004300050011
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02123683
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-017-0501-6
更新日期:2017-06-01 00:00:00
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-002-0109-2
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02121822
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF00191550
更新日期:1992-01-01 00:00:00
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s004300050101
更新日期:1999-08-01 00:00:00
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02125494
更新日期:1978-01-01 00:00:00
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-016-0467-9
更新日期:2016-12-01 00:00:00
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-015-0419-9
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章
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更新日期:2012-08-01 00:00:00
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章
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更新日期:2006-03-01 00:00:00
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF00190308
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章,评审
doi:10.1007/s00430-015-0399-9
更新日期:2015-06-01 00:00:00
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章,评审
doi:10.1007/s00430-012-0264-z
更新日期:2012-11-01 00:00:00
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journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02123563
更新日期:1984-01-01 00:00:00