Abstract:
:The presence of Borna disease virus (BDV)-specific RNA was traced by the reverse transcriptase-polymerase chain reaction in conjunctival fluid, nasal secretions and saliva of horses which were seropositive but did not have any history of clinical Borna disease. Positive reactions encompassed sequences encoding the p24 BDV-specific protein. Virus specificity of the amplified product was confirmed by hybridization with the respective oligomer probe. Viral infectivity or virus-specific antigen was not found in any of these secretions by conventional assays in cell culture and immunoblotting.
journal_name
Med Microbiol Immunoljournal_title
Medical microbiology and immunologyauthors
Richt JA,Herzog S,Haberzettl K,Rott Rdoi
10.1007/BF00191945subject
Has Abstractpub_date
1993-12-01 00:00:00pages
293-304issue
6eissn
0300-8584issn
1432-1831journal_volume
182pub_type
杂志文章abstract::Illness and death of mice infected by intracerebral inoculation with lymphocytic choriomeningitis virus are pathologic immune phenomena, and mice thus infected were protected when immunosuppressed with cyclosporin A (CSA), although the virus multiplied extensively in all major organs. Concentrations remained high as l...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02123695
更新日期:1985-01-01 00:00:00
abstract::A skim-milk fraction and a whey-protein concentrate (WPC) fraction were prepared from the cows that had been immunized with E. coli isolated from the mouse intestine. The antibacterial effect of these fractions against E. coli was examined. They contained antibody with a high affinity for E. coli strain 48, a represen...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF00189427
更新日期:1992-01-01 00:00:00
abstract::The production of thermolabile (LT) enterotoxin was compared in a defined medium reported by Staples et al. (SAG medium) for the production of thermostable (ST) enterotoxin and the Casamino acids-Yeast extract (CAYE) medium. Aliquots were drawn frum cultures of an enterotoxigenic (LT+, ST+) E. coli in both media at di...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02122706
更新日期:1982-01-01 00:00:00
abstract::We have studied the incidence of enterotoxigenic and enteropathogenic Escherichia coli strains associated with infant diarrhoeal disease in Galicia (North-west Spain). During a period of 9 months we isolated heat-labile enterotoxin-positive strains in 2.1% of children with diarrhoea examined, whereas the production of...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02123802
更新日期:1983-01-01 00:00:00
abstract::Carbapenems are important last-line antibiotics for the treatment of hospital infections. Enterobacteriaceae (such as Klebsiella pneumoniae or Escherichia coli) expressing the "New Delhi Metallo-β-lactamase" gene bla(NDM-1) are resistant to carbapenems and were predicted to become a major global health problem. To cop...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-011-0189-y
更新日期:2011-05-01 00:00:00
abstract::Killer cell immunoglobulin like receptors (KIRs) have a principal role in regulating the effector functions of NK cells, particularly in viral infections. The major ligands for KIRs are human leukocyte antigen (HLA) class I molecules. The aim of this study is to investigate the possible association of KIR genes, their...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-017-0522-1
更新日期:2017-12-01 00:00:00
abstract::Escherichia coli isolates of serotype O6:K5 are the most common causative agents of cystitis and pyelonephritis in adults. To answer the question, as to whether strains of this particular serotype represent one special clonal group, out of a collection of 34 serotype O6:K5 isolates [Zingler et al. (1990) Zentralbl. Ba...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF00195947
更新日期:1993-03-01 00:00:00
abstract::Proteus mirabilis (P. mirabilis), a gram-negative enteric bacterium, frequently causes urinary tract infections. Many virulence factors of uropathogenic P. mirabilis have been identified, including urease, flagella, hemolysin and fimbriae. However, the functions of polyphosphate kinase (PPK), which are related to the ...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-015-0430-1
更新日期:2016-04-01 00:00:00
abstract::Gangliosides are known to influence cell growth and differentiation. The neolacto series ganglioside IV3NeuAc-nLc4 (2-->3-sialosylparagloboside) is present in members of the monocyte/granulocyte lineage, but is not found in cells that belong to the lymphocyte lineage. In this study we demonstrated that IV3NeuAc-nLc4 i...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s004300050101
更新日期:1999-08-01 00:00:00
abstract::Vaccination has proven to be one of the best weapons protecting the mankind against infectious diseases. Along with the huge progress in microbiology, numerous highly efficacious and safe vaccines have been produced by conventional technology (cultivation), by the use of molecular biology (genetic modification), or by...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章,评审
doi:10.1007/s00430-014-0346-1
更新日期:2014-12-01 00:00:00
abstract::Recently we demonstrated that lack of mecA transcription was responsible for the decreased methicillin resistance phenotype of strongly slime-negative Staphylococcus epidermidis phase variants [Mempel M, Feucht H, Ziehbuhr W, Endres M, Laufs R, Grüter L (1994) Antimicrob Agents Chemother 38: 1251-1255]. In the present...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF00224346
更新日期:1995-10-01 00:00:00
abstract::Since the dynamics of transmission of human cytomegalovirus (HCMV) have not been clarified yet, we assessed a possible change in HCMV seroprevalence in Frankfurt am Main, Germany during the past twenty years and tried to detect variables with an impact on epidemiology. Between 1/1/1988 and 10/15/2008, a total of 54443...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-009-0136-3
更新日期:2010-02-01 00:00:00
abstract::A coupled luminescent method (CLM) based on glyceraldehyde-3-phosphate dehydrogenase released from injured target cells was used to evaluate the cytotoxicity of antigen-specific HLA class I-restricted CTLs. In contrast to established methods, CLM does not require the pretreatment of target cells with radioactive or to...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-009-0126-5
更新日期:2009-11-01 00:00:00
abstract::This study describes the properties of an inactivated subunit antigen preparation from varicella-zoster virus (VZV)-infected MRC-5 cells by treatment with detergent and formaldehyde, ultracentrifugation over sucrose and acetone precipitation. The method preserved the antigenicity of VZV proteins and several VZV-specif...
journal_title:Medical microbiology and immunology
pub_type: 临床试验,杂志文章
doi:10.1007/BF00277161
更新日期:1994-05-01 00:00:00
abstract::Turkey is seeing a steady rise in rates of HIV infection in the country. The number of individuals with HIV/AIDS was greater than 7000 in 2014 according to data released by the Ministry of Health, and heterosexual contacts were reported to be the main transmission routes. Istanbul has the highest number of reported ca...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-015-0419-9
更新日期:2015-08-01 00:00:00
abstract::Epidemiological studies in Mecklenburg-Vorpommern have shown a high prevalence of Borrelia burgdorferi-infected ticks. A total of 17 B. burgdorferi sensu lato strains were isolated from ticks and investigated by Western blots (immunoblot) with eight monoclonal anti-bodies against different epitopes of the outer surfac...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:
更新日期:1996-02-01 00:00:00
abstract::Neutrophil extracellular traps (NETs) have recently been discovered as a central part of antimicrobial innate immunity. In the meanwhile, evidence accumulated that NETs are also generated upon non-infectious stimuli in various clinical settings. In acute or chronic inflammatory disorders aberrantly enhanced NET format...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章,评审
doi:10.1007/s00430-009-0121-x
更新日期:2009-11-01 00:00:00
abstract::Virus adsorption and uptake of human rhinovirus 14 (HRV14) were studied with HeLa cells and baby hamster kidney (BHK) cells which were transfected with the HRV14 receptor intercellular adhesion molecule-1 (ICAM-1). Transmission electron microscopy of HeLa cells revealed that HRV14 was internalized via clathrin-coated ...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s004300050039
更新日期:1997-06-01 00:00:00
abstract::Earlier we reported that vaccination of leprosy patients with Mycobacterium w induces an immune response directed predominantly against low molecular weight antigens. One of these antigens, with a molecular mass of 30-kDa, was recognized by a majority of the vaccinated subjects as well as the tuberculoid leprosy patie...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF00579623
更新日期:1993-11-01 00:00:00
abstract::Mastitis, which is generally described as an inflammation of breast tissue, is a common and debilitating disease which frequently results in the cessation of exclusive breastfeeding and affects up to 33% of lactating women. The condition is a primary cause of decreased milk production and results in organoleptic and n...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章,评审
doi:10.1007/s00430-017-0532-z
更新日期:2018-04-01 00:00:00
abstract::Enterocytozoon bieneusi is emerging as an important cause of chronic diarrhoea in AIDS patients. Its reservoirs and transmission patterns are unknown. In this study, we have examined E. bieneusi sequences from four Rhesus macaques of different origin, which were kept at one animal facility. The sequences were identica...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-005-0240-y
更新日期:2005-08-01 00:00:00
abstract::Streptococcus pyogenes is a human pathogen which causes a spectrum of diseases ranging from pharyngitis to rheumatic fever, necrotising fasciitis and toxic shock syndrome. Development of a vaccine for S. pyogenes has been confounded both by the diversity of the disease-causing serotypes and the spectre of inadvertentl...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-014-0353-2
更新日期:2015-04-01 00:00:00
abstract::Quorum sensing system is a cell-to-cell communication system that plays a pivotal role in virulence expression in bacteria. Recent advances have demonstrated that the Pseudomonas aeruginosa quorum sensing molecule, N-3-oxododecanoyl homoserine lactone (3OC(12)-HSL), exerts effects on mammalian cells and modulates host...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-009-0111-z
更新日期:2009-05-01 00:00:00
abstract::Virus-induced antigens were isolated from MDV and HVT-infected cells by salt extraction with 3M KCl and purified by Con.A chromatography and isoelectric focusing. Electrophoretic analysis of 35S-methionine, 3H-fucose and 32P-orthophosphate labeled antigens revealed 7 different polypeptides, two of them containing labe...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02126676
更新日期:1977-10-07 00:00:00
abstract::Six colonies producing antibodies were obtained by fusing mouse myeloma SP2-O cells with spleen cells from mice immunized with cytomegalovirus Davis strain. Among 88 surviving clones, 29 produced antibodies detectable by immunofluorescence on infected MRC5 cells and 2 others produced neutralizing antibodies against a ...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02123803
更新日期:1983-01-01 00:00:00
abstract::A method for the purification and concentration of Cytomegalovirus nucleocapsids and particles complete with envelope is described. Antibody to either nucleocapsid or envelope antigens can be detected in human sera. In patients with CMV disease the antibodies to the envelope antigen could not be detected before the 3r...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02121005
更新日期:1975-07-02 00:00:00
abstract::Mycobacterium avium subsp. paratuberculosis (MAP) has long been implicated as a triggering agent in Crohn's disease (CD). In this study, we investigated the growth/persistence of both M. avium subsp. hominissuis (MAH) and MAP, in macrophages from healthy controls (HC), CD and ulcerative colitis patients. For viability...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-015-0393-2
更新日期:2015-12-01 00:00:00
abstract::Serum-starved human foreskin fibroblasts were infected by human cytomegalovirus (Towne strain) that is thought to induce DNA replication in host cells during lytic infection. At various times postinfection, the cultures were pulse labeled with either 3H-thymidine or 14C-thymidine and 3H-lysine to examine DNA synthesis...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02121653
更新日期:1980-02-01 00:00:00
abstract::The new QuantiFERON-TB Gold Plus employs modified peptides optimized to elicit an IFNγ response from CD8+ cytotoxic T lymphocytes in addition to CD4+ T cells. With a view to improve the difficult identification of TB cases, we assessed the combination of two specific immunological markers comprising IFNγ secretion and...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/s00430-018-00576-4
更新日期:2019-04-01 00:00:00
abstract::The prevalence of enterotoxigenic E coli (ETEC) as a pathogenic agent of diarrhoea in the Tel-Aviv (Israel) area was determined, and the isolated E. coli strains characterized. During three periods (summer 1977, summer 1978, and summer 1979), a total of 335 specimens were tested for the presence of E. coli producing L...
journal_title:Medical microbiology and immunology
pub_type: 杂志文章
doi:10.1007/BF02123712
更新日期:1980-01-01 00:00:00