Abstract：
:Carbapenems are important last-line antibiotics for the treatment of hospital infections. Enterobacteriaceae (such as Klebsiella pneumoniae or Escherichia coli) expressing the "New Delhi Metallo-β-lactamase" gene bla(NDM-1) are resistant to carbapenems and were predicted to become a major global health problem. To cope with this emerging threat, there is a need for rapid and sensitive molecular assays to detect bla(NDM-1) in carbapenem-resistant Enterobacteriaceae from clinical isolates. In diagnostic laboratories, real-time PCR is the current gold standard for the sensitive and rapid detection of pathogens. We describe a real-time PCR assay as well as two conventional PCR assays to detect bla(NDM-1). Only minute amounts of total DNA extracted from one bacterial colony are sufficient to allow detection of bla(NDM-1) by real-time PCR within less than 1 h. We also introduce a chemically synthesized bla(NDM-1) gene as a convenient positive control for those laboratories wishing to setup in-house assays for bla(NDM-1) detection. Importantly, our study represents a proof of principle for the usefulness of rapidly synthesized genes serving as positive controls for novel diagnostic PCR assays of emerging pathogens during the initial phase after their discovery when biological isolates are still rare and not commonly available.
journal_name
Med Microbiol Immunoljournal_title
Medical microbiology and immunologyauthors
Krüttgen A,Razavi S,Imöhl M,Ritter Kdoi
10.1007/s00430-011-0189-ysubject
Has Abstractpub_date
2011-05-01 00:00:00pages
137-41issue
2eissn
0300-8584issn
1432-1831journal_volume
200pub_type
杂志文章abstract：:NFR nude (nu/nu) and euthymic (+/nu) littermates were infected with coxsackievirus B3 (CBV-3) and assayed for virus persistence in the heart, pancreas and spleen, for development of myocarditis and for antibody production. The virus grew to higher titer and persisted longer in hearts of nu/nu mice. In both types of mi...
journal_title：Medical microbiology and immunology
pub_type： 杂志文章
doi：10.1007/BF02123563
更新日期：1984-01-01 00:00:00
abstract：:A sentinel surveillance system of the public health service for acute respiratory diseases (ARD), especially influenza, has been realized in the Free State of Saxony of Germany for 25 years. The epidemiological sentinel is based on weekly registration of morbidity and mathematical-statistical calculations. For a micro...
journal_title：Medical microbiology and immunology
pub_type： 杂志文章
doi：10.1007/s00430-002-0136-z
更新日期：2002-12-01 00:00:00
abstract：:A method for the purification and concentration of Cytomegalovirus nucleocapsids and particles complete with envelope is described. Antibody to either nucleocapsid or envelope antigens can be detected in human sera. In patients with CMV disease the antibodies to the envelope antigen could not be detected before the 3r...
journal_title：Medical microbiology and immunology
pub_type： 杂志文章
doi：10.1007/BF02121005
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journal_title：Medical microbiology and immunology
pub_type： 杂志文章
doi：10.1007/BF02122676
更新日期：1981-01-01 00:00:00
abstract：:Lack of virus-specific cell-mediated immunity (CMI) is associated with worse viral infection outcome in hematopoietic stem cell transplantation (HSCT). We aimed to evaluate the role of immunological monitoring of Epstein-Barr virus (EBV) infection in addition to virological one in 33 adult and 18 pediatric allogeneic ...
journal_title：Medical microbiology and immunology
pub_type： 杂志文章
doi：10.1007/s00430-019-00629-2
更新日期：2019-12-01 00:00:00
abstract：:Mycobacterium avium subsp. paratuberculosis (MAP) has long been implicated as a triggering agent in Crohn's disease (CD). In this study, we investigated the growth/persistence of both M. avium subsp. hominissuis (MAH) and MAP, in macrophages from healthy controls (HC), CD and ulcerative colitis patients. For viability...
journal_title：Medical microbiology and immunology
pub_type： 杂志文章
doi：10.1007/s00430-015-0393-2
更新日期：2015-12-01 00:00:00
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journal_title：Medical microbiology and immunology
pub_type： 杂志文章
doi：10.1007/BF00198530
更新日期：1990-01-01 00:00:00
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journal_title：Medical microbiology and immunology
pub_type： 杂志文章
doi：10.1007/s00430-015-0430-1
更新日期：2016-04-01 00:00:00
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journal_title：Medical microbiology and immunology
pub_type： 杂志文章
doi：10.1007/s00430-006-0025-y
更新日期：2007-03-01 00:00:00
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journal_title：Medical microbiology and immunology
pub_type： 杂志文章,评审
doi：10.1007/s00430-015-0396-z
更新日期：2015-06-01 00:00:00
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journal_title：Medical microbiology and immunology
pub_type： 杂志文章
doi：10.1007/s00430-002-0161-y
更新日期：2003-08-01 00:00:00
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journal_title：Medical microbiology and immunology
pub_type： 杂志文章,评审
doi：10.1007/s00430-020-00676-0
更新日期：2020-08-01 00:00:00
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journal_title：Medical microbiology and immunology
pub_type： 杂志文章
doi：10.1007/s00430-016-0457-y
更新日期：2016-10-01 00:00:00
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journal_title：Medical microbiology and immunology
pub_type： 杂志文章
doi：10.1007/BF00200678
更新日期：1987-01-01 00:00:00
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journal_title：Medical microbiology and immunology
pub_type： 杂志文章
doi：10.1007/BF02122710
更新日期：1975-09-19 00:00:00
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journal_title：Medical microbiology and immunology
pub_type： 杂志文章
doi：10.1007/s00430-002-0149-7
更新日期：2003-05-01 00:00:00
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journal_title：Medical microbiology and immunology
pub_type： 杂志文章
doi：10.1007/BF02121319
更新日期：1976-06-01 00:00:00
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journal_title：Medical microbiology and immunology
pub_type： 杂志文章
doi：10.1007/BF00191945
更新日期：1993-12-01 00:00:00
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journal_title：Medical microbiology and immunology
pub_type： 杂志文章
doi：10.1007/BF00203305
更新日期：1989-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00430-018-0554-1
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journal_title：Medical microbiology and immunology
pub_type： 历史文章,杂志文章,评审
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更新日期：2015-06-01 00:00:00
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journal_title：Medical microbiology and immunology
pub_type： 杂志文章
doi：10.1007/BF02121653
更新日期：1980-02-01 00:00:00
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journal_title：Medical microbiology and immunology
pub_type： 杂志文章
doi：10.1007/s004300050025
更新日期：1996-11-01 00:00:00
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journal_title：Medical microbiology and immunology
pub_type： 杂志文章
doi：10.1007/s00430-009-0136-3
更新日期：2010-02-01 00:00:00
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journal_title：Medical microbiology and immunology
pub_type： 杂志文章
doi：10.1007/BF02123802
更新日期：1983-01-01 00:00:00
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journal_title：Medical microbiology and immunology
pub_type： 杂志文章
doi：10.1007/BF02123624
更新日期：1982-01-01 00:00:00
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journal_title：Medical microbiology and immunology
pub_type： 杂志文章
doi：10.1007/BF02125527
更新日期：1981-01-01 00:00:00
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journal_title：Medical microbiology and immunology
pub_type： 杂志文章,评审
doi：10.1007/s00430-009-0121-x
更新日期：2009-11-01 00:00:00
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journal_title：Medical microbiology and immunology
pub_type： 杂志文章
doi：10.1007/s004300050011
更新日期：1996-05-01 00:00:00
abstract：:The aroB gene from Helicobacter pylori strain P1 was cloned and further characterized by sequence analysis and by functional complementation of the aroB mutation in Escherichia coli. The aroB gene encodes the enzyme 3-dehydroquinate synthase which catalyzes one of the early steps in the shikimate pathway. This pathway...
journal_title：Medical microbiology and immunology
pub_type： 杂志文章
doi：10.1007/s004300050054
更新日期：1997-10-01 00:00:00
