Abortive growth of human lymphocytes carrying a dormant Epstein-Barr viral genome.

abstract：:We assessed whether the intracellular bacterium Chlamydia pneumoniae was present in post-mortem brain samples from patients with and without late-onset Alzheimer's disease (AD), since some indirect evidence seems to suggest that infection with the organism might be associated with the disease. Nucleic acids prepared f...

journal_title：Medical microbiology and immunology

authors： Balin BJ,Gérard HC,Arking EJ,Appelt DM,Branigan PJ,Abrams JT,Whittum-Hudson JA,Hudson AP

更新日期：1998-06-01 00:00:00

abstract：:The present study describes the respiratory immune response of mice to locally administered antigen, and the modulation of this response by systemic immunization. Intranasal immunization of mice with the A/PR/8/34 strain of influenza virus evoked local antibody response of the IgA type. The titer of the IgA antibodies...

journal_title：Medical microbiology and immunology

authors： Zakay-Rones Z,Hadas O,Levy R

更新日期：1978-11-17 00:00:00

abstract：:Upon infection of human T-cell leukemia virus type I (HTLV-I)-carrying human T-cell lines such as MT-4, HTLV-III, a probable etiologic agent of acquired immune deficiency syndrome (AIDS) caused fast and strong cytopathic effects leading ultimately to the death of the cells. Such effects were preceded by the rapid indu...

journal_title：Medical microbiology and immunology

authors： Nakashima H,Koyanagi Y,Harada S,Yamamoto N

更新日期：1986-01-01 00:00:00

abstract：:Lack of virus-specific cell-mediated immunity (CMI) is associated with worse viral infection outcome in hematopoietic stem cell transplantation (HSCT). We aimed to evaluate the role of immunological monitoring of Epstein-Barr virus (EBV) infection in addition to virological one in 33 adult and 18 pediatric allogeneic ...

journal_title：Medical microbiology and immunology

authors： Chiereghin A,Piccirilli G,Belotti T,Prete A,Bertuzzi C,Gibertoni D,Gabrielli L,Turello G,Borgatti EC,Barbato F,Sessa M,Arpinati M,Bonifazi F,Lazzarotto T

更新日期：2019-12-01 00:00:00

abstract：:A coupled luminescent method (CLM) based on glyceraldehyde-3-phosphate dehydrogenase released from injured target cells was used to evaluate the cytotoxicity of antigen-specific HLA class I-restricted CTLs. In contrast to established methods, CLM does not require the pretreatment of target cells with radioactive or to...

journal_title：Medical microbiology and immunology

authors： Ogbomo H,Geiler J,Leutz A,von Kietzell K,Michaelis M,Doerr HW,Cinatl J Jr

更新日期：2009-11-01 00:00:00

abstract：:Since the dynamics of transmission of human cytomegalovirus (HCMV) have not been clarified yet, we assessed a possible change in HCMV seroprevalence in Frankfurt am Main, Germany during the past twenty years and tried to detect variables with an impact on epidemiology. Between 1/1/1988 and 10/15/2008, a total of 54443...

journal_title：Medical microbiology and immunology

authors： Lübeck PR,Doerr HW,Rabenau HF

更新日期：2010-02-01 00:00:00

abstract：:This study describes the properties of an inactivated subunit antigen preparation from varicella-zoster virus (VZV)-infected MRC-5 cells by treatment with detergent and formaldehyde, ultracentrifugation over sucrose and acetone precipitation. The method preserved the antigenicity of VZV proteins and several VZV-specif...

journal_title：Medical microbiology and immunology

authors： Davies J,Hallworth JA,McLeish P,Randall S,Martin BA,Buchan A,Skinner GR

更新日期：1994-05-01 00:00:00

abstract：:The pathogenesis of Staphylococcus aureus from local infection to systemic dissemination involves a range of virulence factors including structural and secreted products. Among various control mechanisms, small noncoding RNAs are involved in the regulation of multiple pathogenicity factors in S. aureus. The sRNA SprX ...

journal_title：Medical microbiology and immunology

authors： Kathirvel M,Buchad H,Nair M

更新日期：2016-12-01 00:00:00

abstract：:Recently we demonstrated that lack of mecA transcription was responsible for the decreased methicillin resistance phenotype of strongly slime-negative Staphylococcus epidermidis phase variants [Mempel M, Feucht H, Ziehbuhr W, Endres M, Laufs R, Grüter L (1994) Antimicrob Agents Chemother 38: 1251-1255]. In the present...

journal_title：Medical microbiology and immunology

authors： Mempel M,Müller E,Hoffmann R,Feucht H,Laufs R,Grüter L

更新日期：1995-10-01 00:00:00

abstract：:IgG immune response to CagA was evaluated by enzyme-linked imunosorbent assay (ELISA) using a recombinant fragment of CagA as antigen in 171 patients with gastric cancer and 298 blood donors to determine whether it could be related to the ABO(H) blood group phenotype, stage of cancer or tumor morphology. The CagA-ELIS...

journal_title：Medical microbiology and immunology

authors： Klaamas K,Kurtenkov O,Covacci A,Lipping A,Wadström T

更新日期：1999-05-01 00:00:00

abstract：:A skim-milk fraction and a whey-protein concentrate (WPC) fraction were prepared from the cows that had been immunized with E. coli isolated from the mouse intestine. The antibacterial effect of these fractions against E. coli was examined. They contained antibody with a high affinity for E. coli strain 48, a represen...

journal_title：Medical microbiology and immunology

authors： Nomoto K,Matsuoka Y,Hayakawa K,Ohwaki M,Kan T,Yoshikai Y,Nomoto K

更新日期：1992-01-01 00:00:00

abstract：:The purpose of this study was to characterize antigenic determinants on structural polypeptides of human immunodeficiency virus type 2 (HIV-2ben). Therefore, three HIV-2-specific monoclonal antibodies (mAbs) against the p24 core protein (gag) and one mAb against the gp130 envelope glycoprotein (env) were produced. In ...

journal_title：Medical microbiology and immunology

authors： Faisst AC,Otteken A,Nick S,Hunsmann G

更新日期：1993-07-01 00:00:00

abstract：:Outer membrane fractions (OMs) of nine Campylobacter (C.) jejuni and two C. coli strains belonging to different serovars, from human and various animal origins, were extracted by treatment with sodium N-lauryl sarcosinate. Using n-octyl-beta-D-glucopyranoside a 42-kDa protein and a flagella-enriched fraction were obta...

journal_title：Medical microbiology and immunology

authors： Moser I,Schröder WF,Hellmann E

更新日期：1992-01-01 00:00:00

abstract：:EBV-associated polypeptides induced in different Epstein-Barr Virus (EBV)-producing cell lines by the tumor promotor TPA, and from purified EBV particles derived from the same lines were radioactivity labeled and analyzed by immunoprecipitation with human VCA+MA+ sera. In virus-producing cells no significant differenc...

journal_title：Medical microbiology and immunology

authors： Georg-Fries B,Mueller-Lantzsch N

更新日期：1982-01-01 00:00:00

abstract：:Virus-induced antigens were isolated from MDV and HVT-infected cells by salt extraction with 3M KCl and purified by Con.A chromatography and isoelectric focusing. Electrophoretic analysis of 35S-methionine, 3H-fucose and 32P-orthophosphate labeled antigens revealed 7 different polypeptides, two of them containing labe...

journal_title：Medical microbiology and immunology

authors： Kaaden OR

更新日期：1977-10-07 00:00:00

abstract：:In the present study, synthetic peptides (EENVEHDA)2 [(oc)2] and (DDEHVEEPTVA)2 [(un)2] of ring-infected erythrocyte surface antigen (RESA) of Plasmodlium filciparum were linked with palmitic acid and entrapped in immunostimulating complexes (ISCOMs). The immunogenicity of the peptide(s) and mixture of peptides were s...

journal_title：Medical microbiology and immunology

authors： Chopra N,Biswas S,Thomas B,Sabhnani L,Rao DN

更新日期：2000-11-01 00:00:00

abstract：:Surface proteins of nine Campylobacter jejuni strains belonging to three different serovia were extracted with lysozyme/ethylenediamine-tetraacetic acid. The preparations bound to isolated murine small intestinal cells and to a membrane fraction (MF) of isolated brush borders obtained by detergent treatment with Trito...

journal_title：Medical microbiology and immunology

authors： Moser I,Hellmann E

更新日期：1989-01-01 00:00:00

abstract：:A 33-kDa protein (TB33) was isolated from a delipidated cell sonicate (CS) of Mycobacterium tuberculosis H37Rv (grown in Middlebrook 7H9 broth supplemented with glucose) using immobilized metal affinity chromatography (IMAC) on a nickel-nitrilotriacetic acid (Ni-NTA) column. TB33 could not be isolated from the culture...

journal_title：Medical microbiology and immunology

authors： Deshpande RG,Khan MB,Bhat DA,Navalkar RG

更新日期：1996-11-01 00:00:00

abstract：:The present study analyses the susceptibility of human bladder-derived cells (HT-1376) to the infection by herpes simplex virus type 2 (HSV-2) and Chlamydia trachomatis, as well as to the adhesiveness of uropathogenic bacteria. HT-1376 cells were efficiently infected by HSV-2 strain 333, as demonstrated by immunofluor...

journal_title：Medical microbiology and immunology

authors： Superti F,Longhi C,Di Biase AM,Tinari A,Marchetti M,Pisani S,Gallinelli C,Chiarini F,Seganti L

更新日期：2001-09-01 00:00:00

abstract：:A clinical trial was conducted with Alice/R-75 strain live attenuated intranasal influenza A/B vaccine. With double blind control 88 adult volunteers were administered 2 doses of Alice/R-75 vaccine, 93 volunteers received one dose of Alice/R-75 vaccine and one dose placebo solution and 94 subjects were administered 2 ...

journal_title：Medical microbiology and immunology

authors： Spencer MJ,Cherry JD,Powell KR,Sumaya CV

更新日期：1979-03-13 00:00:00

abstract：:Cytotoxic effects of a leukocidin from Fusobacterium necrophorum were demonstrated on bovine hepatic cells. The cytotoxic response was dose-dependent and could be inhibited by homologous antiserum. Scanning electron microscopy revealed damaged hepatic cell surface. These findings indicated a pathogenic role of the leu...

journal_title：Medical microbiology and immunology

authors： Ishii T,Kanoe M,Inoue T,Kai K,Blobel H

更新日期：1988-01-01 00:00:00

abstract：:Virus adsorption and uptake of human rhinovirus 14 (HRV14) were studied with HeLa cells and baby hamster kidney (BHK) cells which were transfected with the HRV14 receptor intercellular adhesion molecule-1 (ICAM-1). Transmission electron microscopy of HeLa cells revealed that HRV14 was internalized via clathrin-coated ...

journal_title：Medical microbiology and immunology

authors： Grunert HP,Wolf KU,Langner KD,Sawitzky D,Habermehl KO,Zeichhardt H

更新日期：1997-06-01 00:00:00

abstract：:Low public awareness of cytomegalovirus (CMV) results from the only mild and transient symptoms that it causes in the healthy immunocompetent host, so that primary infection usually goes unnoticed. The virus is not cleared, however, but stays for the lifetime of the host in a non-infectious, replicatively dormant stat...

journal_title：Medical microbiology and immunology

authors： Seckert CK,Griessl M,Büttner JK,Scheller S,Simon CO,Kropp KA,Renzaho A,Kühnapfel B,Grzimek NK,Reddehase MJ

更新日期：2012-11-01 00:00:00

abstract：:Phosphorylcholine (ChoP) is covalently incorporated into bacterial surface structures, contributing to host mimicry and promoting adhesion to surfaces. Our aims were to determine the frequency of ChoP display among Aggregatibacter actinomycetemcomitans strains, to clarify which surface structures bear ChoP, and whethe...

journal_title：Medical microbiology and immunology

authors： Ihalin R,Zhong D,Karched M,Chen C,Asikainen S

更新日期：2018-11-01 00:00:00

abstract：:The infant rat model is widely used to study the pathogenesis of meningitis caused by a variety of gram-positive and gram-negative bacteria. However, the interpretation of published results concerning meningitis is difficult in many records because the fact that blood contamination of the cerebrospinal fluid (CSF) can...

journal_title：Medical microbiology and immunology

authors： Vogel U,Steinmetz I,Frosch M

更新日期：1996-05-01 00:00:00

abstract：:The generation of rodent Plasmodium strains expressing fluorescent proteins in all life cycle stages has had a big impact on malaria research. With this tool in hand, for the first time it was possible to follow in real time by in vivo microscopy the infection route of Plasmodium sporozoites transmitted to the mammali...

journal_title：Medical microbiology and immunology

authors： Sturm A,Heussler V

更新日期：2007-09-01 00:00:00

abstract：:The efficiency was examined of immunization with feline leukemia virus glycoprotein complexes (gp85 rosettes) to protect cats against tumors induced by feline sarcoma virus (FeSV). The glycoprotein was isolated from feline leukemia virus (FeLV). Young cats were vaccinated with the purified viral glycoprotein and chall...

journal_title：Medical microbiology and immunology

authors： Hunsmann G,Pedersen NC,Theilen GH,Bayer H

更新日期：1983-01-01 00:00:00

abstract：:Due to its ability to attach to polymeric surfaces Staphylococcus epidermidis is a common pathogen in chronic, medical device-associated infections. Attached S. epidermidis displays reduced susceptibility against a variety of antimicrobial substances, and little correlation between standard susceptibility test results...

journal_title：Medical microbiology and immunology

authors： Knobloch JK,Von Osten H,Horstkotte MA,Rohde H,Mack D

更新日期：2002-10-01 00:00:00

abstract：:Interactions of virulent Leptospira interrogans with murine monocyte-macrophage-like J774A.1 cells and Vero (African green monkey kidney fibroblasts) cells from attachment to internalization were investigated by a series of morphological analysis. Fontana silver staining revealed that only the pathogenic leptospires w...

journal_title：Medical microbiology and immunology

authors： Liu Y,Zheng W,Li L,Mao Y,Yan J

更新日期：2007-12-01 00:00:00

abstract：:Antibody to recombinant hepatitis C virus (HCV) protein C100 (anti-C100) was measured for a period of 6 months by enzyme immunoassay in nine prospectively followed non A-nonB (NANBH) cases which occurred after cardiac surgery at a hospital in Rio de Janeiro (Brazil). At least seven cases were infected with HCV; four o...

journal_title：Medical microbiology and immunology

authors： Yoshida CF,Soares OA,Schatzmayr HG,Uy A,Gerlich WH

更新日期：1992-01-01 00:00:00