Abstract:
PURPOSE:We have found that two aspartyl (Asp-151 and Asp-58) residues of alphaA-crystallin are inverted and isomerized to the biologically uncommon D-beta-Asp residues during aging. In order to elucidate the correlation between the formation of the D-beta-Asp isomer and the environment surrounding the Asp in the protein, we performed a Raman spectroscopic study using two synthetic peptides: T6 peptide containing Asp-58, and T18 peptide, containing Asp-151, which correspond to the tryptic peptides of human alphaA-crystallin. METHODS:Both T6 (Thr-Val-Leu-Asp(58)-Ser-Gly-Ile-Ser-Glu-Val-Arg) and T18 (Ile-Gln-Thr-Gly-Leu-Asp(151)-ala-thr-his-ala-Glu-Arg) peptides were synthesized with four optical isomers which have L-alpha-, D-alpha, L-beta and D-beta-aspartyl residues. These peptides were subjected to Raman measurement. RESULTS:The Raman spectrum of the L-alpha-Asp T18 peptide measured as dry powder revealed that the secondary structure of this peptide is mainly anti-parallel beta-sheet. The main structure of the D-beta-Asp T18 peptide when in dry powder form was altered to an alpha-helix and/or random structure. The main structure of L-alpha-Asp T18 peptide when measured in aqueous solution also converted to an alpha-helix and/or random structure. The conversion of L-alpha-to D-beta-Asp in T6 peptides when in dry powder form revealed no alteration of secondary beta-sheeted structure. CONCLUSION:Raman spectroscopy clearly revealed a large conformational change in the secondary structure of T18 peptide caused by substitution of normal L-alpha-Asp to biologically uncommon Asp-isomers. This result indicates that the inversion of an amino acid in a protein greatly affects the secondary structure of the protein.
journal_name
Jpn J Ophthalmoljournal_title
Japanese journal of ophthalmologyauthors
Mizuno A,Fujii N,Akaboshi M,Momose Y,Shimo-oka Tdoi
10.1016/s0021-5155(00)00202-1keywords:
subject
Has Abstractpub_date
2000-07-01 00:00:00pages
354-9issue
4eissn
0021-5155issn
1613-2246pii
S0021-5155(00)00202-1journal_volume
44pub_type
杂志文章abstract:PURPOSE:To determine whether P7C3-A20, a proneurogenic neuroprotective agent, can protect the retinal ganglion cells (RGCs) of rats from optic nerve crushing. METHODS:The left optic nerve of 67 rats was crushed, and 5.0 mg/kg/day of P7C3-A20 (crush-P7C3) or its vehicle (crush-placebo) was injected intraperitoneally fo...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-016-0493-6
更新日期:2017-03-01 00:00:00
abstract:BACKGROUND:Interferon (IFN)-associated retinopathy is typically characterized by retinal hemorrhages and cotton wool spots at the posterior fundus, but visual function is usually maintained. With the use of optical coherence tomography (OCT), two patients with IFN-associated retinopathy who had developed macular edema ...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-004-0176-6
更新日期:2005-05-01 00:00:00
abstract:PURPOSE:To test the hypothesis that ischemia of the Schlemm's canal endothelium is involved in the control of intraocular pressure (IOP). METHODS:Experiments were conducted on pigeons. The course of the aqueous sinus and the aqueous sinus artery was determined by examining serial sections and the distribution of polym...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0021-5155(99)00017-9
更新日期:1999-07-01 00:00:00
abstract:PURPOSE:We investigated the keratocyte response and stromal remodeling after corneal incision and photorefractive keratectomy to understand the histophysiological and immunohistochemical differences between these two types of surgery. METHODS:Corneal incision or photorefractive keratectomy was performed in rabbits or ...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0021-5155(00)00269-0
更新日期:2000-11-01 00:00:00
abstract::Surgically resected subretinal proliferative tissue (SRP) in proliferative vitreoretinopathy was examined by morphological and immunohistochemical methods. Membranous and strand-like SRPs were observed. Membranous SRP (4 cases) consisted of retinal pigment epithelial cells (RPEs) and a thin layer of extracellular mate...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1992-01-01 00:00:00
abstract::Regulation of fluid flow through corneal stroma was investigated in the bullfrog. Corneal specimens were mounted by clamping their limbal sclera between the two chambers of a Ussing-type chamber. The epithelial surface was covered with Ringer's solution, while the endothelial surface was superfused with Ringer's solut...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0021-5155(97)00111-1
更新日期:1998-01-01 00:00:00
abstract::The exfoliation syndrome is thought to be a very rare disease in China. The clinical features of 11 patients with pseudoexfoliation (PE) and the histopathologic changes in specimens affected by PE were studied. The loss of pigment at the pupillary ruff and the dense pigmentation on the trabecular meshwork were the ear...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract:PURPOSE:To describe the clinical features and treatment outcomes of severe retinopathy in eyes with incontinentia pigmenti (IP) of infants within a few months of birth. STUDY DESIGN:Retrospective clinical study. METHODS:Six eyes of three patients (6-day-old girl, 5-month-old girl, and 14-day-old boy) with IP were exa...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-020-00768-7
更新日期:2020-11-01 00:00:00
abstract:PURPOSE:To investigate whether retinal glial cells (RGCs), which are believed to play an important role in the development and maintenance of microvessels, stimulate the proliferation of retinal bovine microvascular pericytes, an essential component of the vessels. METHODS:Conditioned medium (CM) was collected from a ...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0021-5155(02)00527-0
更新日期:2002-07-01 00:00:00
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journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-019-00658-7
更新日期:2019-05-01 00:00:00
abstract:PURPOSE:To examine the correlation between the van Herick system and the Shaffer system in a large-scale epidemiological survey conducted on healthy Japanese subjects. METHODS:The van Herick technique was performed on 14,779 subjects aged 40 years and over who participated in an ophthalmic epidemiological survey condu...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-004-0169-5
更新日期:2005-03-01 00:00:00
abstract:PURPOSE:Sweet's syndrome (SS) is a skin disorder clinically characterized by fever, neutrophilia, and painful edematous plaques that occasionally causes posterior uveitis. We present two cases of SS with panuveitis resembling Behçet's disease (BD). SUBJECTS:Two patients with panuveitis associated with SS. OBSERVATION...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-012-0129-4
更新日期:2012-05-01 00:00:00
abstract:BACKGROUND:Vogt-Koyanagi-Harada (VKH) disease patients with the complication of subretinal pigmented proliferative tissue tend to have a poor visual prognosis. CASE:We herein report a case of VKH with good visual acuity despite a prominent subretinal fold. OBSERVATIONS:A 24-year-old woman, who experienced several rec...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0021-5155(03)00146-1
更新日期:2003-11-01 00:00:00
abstract:PURPOSE:To assess the proportion of glaucomatous eyes based on the association between optic disc (OD) size and its clinical determinants in the resident population of Namil-myon, Central South Korea. METHODS:Of the 1,532 participants in the Namil study, we included 1,410 Koreans (2,734 eyes) with legible fundus photo...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-014-0303-y
更新日期:2014-03-01 00:00:00
abstract:PURPOSE:We report here a patient with bilateral papillomacular retinoschisis with an enlarged glaucomatous optic nerve head cup and a focal lamina cribrosa defect, the findings of our clinical investigations of this case, and the chosen treatment and outcome. DESIGN:This is an observational case report. METHODS:Clini...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-014-0330-8
更新日期:2014-09-01 00:00:00
abstract::A 76-year-old Japanese woman had suffered from fatigue, weight loss, and cutaneous hyperpigmentation at the age of 38 years and was diagnosed as having tuberculous Addison's disease. Since then, corticosteroids had been administered effectively as hormonal replacement. At the age of 75 years, the patient presented wit...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0021-5155(98)00024-0
更新日期:1998-09-01 00:00:00
abstract::An 18-year-old Japanese boy with optic disk drusen associated with left central retinal artery occlusion was reported. Transient central blurred vision, possibly caused by central retinal artery spasm, was noticed as an initial symptom of optic disk drusen in the left eye. Prolonged and repeated spasm might have given...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1982-01-01 00:00:00
abstract:PURPOSE:To evaluate the effect of preservative-free (PF) tafluprost on diurnal variation of intraocular pressure (IOP) and ocular perfusion pressure (OPP), measured by use of home IOP and blood-pressure (BP) monitoring devices, for primary open angle glaucoma (POAG) patients. METHODS:Twenty-two eyes from 22 patients w...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-015-0413-1
更新日期:2016-01-01 00:00:00
abstract:PURPOSE:To investigate the process of repair in the epithelial cells and the reconstruction in lens fibers of a mouse lens that has developed opacity because of a large-scale perforating injury. METHODS:Lenses of 4-week-old mice were perforated with a needle through the cornea to induce the development of traumatic ca...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0021-5155(03)00075-3
更新日期:2003-07-01 00:00:00
abstract::The usefulness of the Fluorotron Master fluorophotometer fitted with a small animal adapter was evaluated with tree shrews. We also determined that the optimal measurement parameters for these animals, which are regarded as promising for experimental use in ophthalmic research, are: fluorescein-Na concentration, 0.5 x...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
abstract::Purpose: A young patient of ophthalmoplegic migraine with recurrent oculomotor nerve palsy is reported.Case: A 4-year-old girl came to our hospital complaining of recurrent left ptosis and double vision which completely resolved within a few days. She had a history of drowsiness after vomiting every two months. Left o...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0021-5155(00)00233-1
更新日期:2000-09-01 00:00:00
abstract:PURPOSE:To determine changes of visual-field (VF) global indices after cataract surgery and the factors associated with the effect of cataracts on those indices in primary open-angle glaucoma (POAG) patients. METHODS:A retrospective chart review of 60 POAG patients who had undergone phacoemulsification and intraocular...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-016-0467-8
更新日期:2016-11-01 00:00:00
abstract:PURPOSE:To determine the relationship between apoptotic photoreceptor cell death and the duration of light exposure. METHODS:Ten-week-old male albino rats (Wistar strain) were dark-adapted for 2 days and then exposed to intense light for 12 hours, and 1, 2, 3, 7, 14, 21, and 28 days. The presence of apoptosis was conf...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0021-5155(99)00011-8
更新日期:1999-05-01 00:00:00
abstract::We explored the involvement of CD44 isoforms in lymphoproliferative disorder (LPD) of the lacrimal gland of a Sjögren's syndrome patient with unilateral LPD. The CD44 variant with the v6 exon was selectively detected from infiltrating lymphocytes in the gland with LPD, but not from infiltrating lymphocytes in the norm...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0021-5155(97)00072-5
更新日期:1997-09-01 00:00:00
abstract:PURPOSE:To determine whether exposure to ozonated solution alters the morphology of corneal endothelial cells in rats and to examine the protective effect of ascorbic acid. METHODS:The anterior chambers of rat eyes were filled with 4 ppm of ozonated solution. Some were left in that state, while others were flushed out...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-008-0629-4
更新日期:2009-03-01 00:00:00
abstract::A surgically obtained lens from a 66-year-old man with the Weill-Marchesani syndrome was examined histopathologically by light microscopy, transmission electron microscopy and scanning electron microscopy. The lens was diffusely opaque and brown. It was microspherophakic in appearance, 6.0 mm in equatorial diameter an...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00
abstract::It is unknown whether aqueous flare is increased in eyes with prominent fundus lesions other than choroidal malignant melanoma. Therefore, we studied the aqueous flare in eyes with age-related macular degeneration (ARMD) using the laser flare-cell meter. Forty-three eyes of 27 patients with ARMD (age 67.0 +/- 14.0 yea...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1994-01-01 00:00:00
abstract:PURPOSE:To investigate the prevalence of superior segmental optic nerve hypoplasia in Korea. METHODS:We reviewed the fundus photographs of 3905 individuals who were 40 years old or older and who participated in the comprehensive medical examination program at the Health Promotion Center of Seoul National University Bu...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-009-0663-x
更新日期:2009-05-01 00:00:00
abstract::Hematoxylin-eosin staining, the TUNEL method for in situ detection of the intranuclear DNA fragmentation, which indicates apoptosis, and electron microscopy were used to study the morphologic changes in specimens from the eyes of 8 patients with secondary glaucoma and 2 normal control eyes to evaluate our hypothesis t...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0021-5155(97)00013-0
更新日期:1997-03-01 00:00:00
abstract:PURPOSE:To investigate the expression of beta-defensin in the ocular surface of a mouse experimental allergic conjunctivitis (EAC) model. METHODS:Mouse beta-defensin (mBD)-1 and -2 mRNA expressed in conjunctival tissue of both the EAC and the control group were analyzed by real-time reverse transcriptase-polymerase ch...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-005-0262-4
更新日期:2006-01-01 00:00:00