Abstract：
PURPOSE:To investigate the process of repair in the epithelial cells and the reconstruction in lens fibers of a mouse lens that has developed opacity because of a large-scale perforating injury. METHODS:Lenses of 4-week-old mice were perforated with a needle through the cornea to induce the development of traumatic cataracts. Over the period from the first day to the fifth month after injury, eyeballs were extracted progressively from the mice and changes in the epithelial cells of these lenses were observed morphologically as well as histochemically. RESULTS:Following the injury, the epithelial cells of the lenses extended toward the center of the injury, while undergoing repeated proliferation and stratification. After a month, the epithelial cells completely covered the entire injury. Later, a basal lamina and collagen fibers developed among the epithelial cells that had proliferated, the intracellular space enlarged, but the number of cells decreased. Histochemically, a strong actin-positive finding was observed in the epithelial cells in the growth phase. On the other hand, an investigation by means of the TUNEL method revealed epithelial cell death and a decrease in cell number. Maximal cell death was observed in the second month. During this period, lens fibers regenerated, and the clear areas of the cortex increased. CONCLUSION:Although the lenses exhibited opacity over a large area, the epithelial cells eventually fully covered the injured area. Once the repair was completed, the number of epithelial cells decreased. At the same time, the lenses were found to have developed increased clarity, leading to reconstruction. The epithelial recovery and the residual posterior suture may be the key to the reconstruction of the lenses.
journal_name
Jpn J Ophthalmoljournal_title
Japanese journal of ophthalmologyauthors
Hirayama S,Wakasugi A,Morita T,Sakai Y,Mukuno K,Uga S,Shimizu Kdoi
10.1016/s0021-5155(03)00075-3keywords:
subject
Has Abstractpub_date
2003-07-01 00:00:00pages
338-46issue
4eissn
0021-5155issn
1613-2246pii
S0021515503000753journal_volume
47pub_type
杂志文章abstract：:Characteristics of the distribution of cytoskeletal and contractile elements in cultured human trabecular cells were studied using the immunofluorescence method. Actin microfilaments exhibited stress fiber images which were observed throughout the cell body in a parallel pattern and were concentrated in the cell perip...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：PURPOSE:To evaluate dry eye symptoms, tear film function and ocular surface changes in patients with psoriasis. METHODS:The Dry Eye Questionnaire and ophthalmic examination including the Schirmer test, tear break-up time, corneal fluorescein test, meibomian gland obstruction and conjunctival impression cytology were a...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1007/s10384-012-0226-4
更新日期：2013-07-01 00:00:00
abstract：PURPOSE:From an early stage, retinitis pigmentosa (RP) patients suffer from night blindness which causes nocturnal mobility difficulties. We created a wearable visual aid that uses a high-performance see-through display, and added a high-sensitivity camera with a complementary metal-oxide-semiconductor sensor. Here, we...
journal_title：Japanese journal of ophthalmology
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journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0021-5155(01)00322-7
更新日期：2001-05-01 00:00:00
abstract：PURPOSE:We have found that two aspartyl (Asp-151 and Asp-58) residues of alphaA-crystallin are inverted and isomerized to the biologically uncommon D-beta-Asp residues during aging. In order to elucidate the correlation between the formation of the D-beta-Asp isomer and the environment surrounding the Asp in the protei...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0021-5155(00)00202-1
更新日期：2000-07-01 00:00:00
abstract：PURPOSE:To investigate the recovery of short-wavelength sensitivity in patients who had undergone successful reattachment of a rhegmatogenous retinal detachment with macular involvement. METHODS:Postoperative assessment of the visual acuity and the visual fields by standard achromatic automated perimetry (SAP), and sh...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1007/s10384-005-0201-4
更新日期：2005-07-01 00:00:00
abstract：:We assessed the function of Endothelin-1 (ET-1) in the development of anterior chamber inflammation in pigmented rabbit eyes. After the injection of ET-1 solution (10(-13), 10(-11), 10(-9), or 10(-7) M, diluted with 300 microL of artificial aqueous humor) into the anterior chamber, the aqueous protein concentration (A...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0021-5155(97)00032-4
更新日期：1997-05-01 00:00:00
abstract：PURPOSE:To evaluate the changes in aqueous flare in psoriasis patients and to identify the factors that influence the level of aqueous flare. METHODS:We examined the 68 eyes of 34 psoriasis patients and the 68 eyes of 34 healthy subjects with a laser flare-cell meter. Complete dermatologic and ophthalmic examinations ...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0021-5155(00)00359-2
更新日期：2001-03-01 00:00:00
abstract：PURPOSE:To identify any possible determinants in the development of uveitis in leprosy patients. METHODS:Human leukocyte antigen (HLA) class I and II antigen, and HLA class II genotypings were analyzed among Japanese leprosy patients. Ninety-three unrelated Japanese leprosy patients (46 patients with a history of uvei...
journal_title：Japanese journal of ophthalmology
pub_type： 临床试验,杂志文章,随机对照试验
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更新日期：1999-03-01 00:00:00
abstract：:We studied family members of 9 patients with falciform retinal fold, and found a number of cases showing features of familial exudative vitreoretinopathy (FEVR) in the fundi. Retinal fold was also seen in the eye of a cousin of the propositus. Three cases with falciform retinal fold were bilateral and 7 cases were uni...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：
更新日期：1983-01-01 00:00:00
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journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1007/s10384-017-0511-3
更新日期：2017-07-01 00:00:00
abstract：:The effect of pertussis toxin (PT) on experimental autoimmune uveoretinitis (EAU) induced by immunization with S-antigen was examined in rats. Intravenous administration of PT (2 micrograms/rat) initiated the development of EAU in rats that had been made resistant to the induction of EAU by immunization with S-antigen...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:Full-field electroretinograms (ERGs) provide a basis for establishing the diagnosis of widespread progressive forms of retinitis pigmentosa in early life even at a time when fundus abnormalities visible with the ophthalmoscope are minimal or absent. Patients characteristically show delays in rod or cone b-wave implici...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章,评审
doi：
更新日期：1987-01-01 00:00:00
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journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：
更新日期：1988-01-01 00:00:00
abstract：PURPOSE:To perform a retrospective study to evaluate the long-term outcome of systemic cyclosporine treatment as an adjunct to topical corticosteroid treatment after penetrating keratoplasty (PKP). METHODS:Twenty-six high-risk patients (27 eyes) who received systemic cyclosporine following PKP for an average of 5.4 mo...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0021-5155(01)00339-2
更新日期：2001-07-01 00:00:00
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journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0021-5155(00)00270-7
更新日期：2000-11-01 00:00:00
abstract：PURPOSE:To assess the combined estimate of retinal ganglion cell (RGC) count developed by Medeiros et al. as a tool for diagnosis of glaucoma in Japanese patients. STUDY DESIGN:Cross-sectional study. METHODS:Thirty-one eyes of 19 healthy controls and 106 eyes of 70 glaucoma patients underwent standard automated perim...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1007/s10384-017-0540-y
更新日期：2018-01-01 00:00:00
abstract：:Clinical, pathological and follow-up data on the 1,147 cases of retinoblastoma registered in Japan from 1975 to 1982 were statistically analyzed. The cumulative survival rate of the total 1,147 cases was 93.0% at 5 years and 90.3% at 10 years, calculated by the Kaplan-Meier method. The survival rate for the 757 unilat...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：
更新日期：1992-01-01 00:00:00
abstract：PURPOSE:We investigated the efficacy of the tear sampling method using a filter paper to evaluate eosinophil cationic protein (ECP) levels in patients with allergic disorders. METHODS:Subjects were an allergic group comprising patients with allergic conjunctivitis, vernal keratoconjunctivitis, or atopic keratoconjunct...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0021-5155(02)00630-5
更新日期：2003-01-01 00:00:00
abstract：:A computerized digital image analysis system was developed to evaluate the retinal nerve fiber layer (RNFL) by red-free fundus photography. The image intensity of the RNFL was computed for 30 degrees between the disc and macula. Thirty early open-angle glaucoma patients and 30 normal age-matched subjects were examined...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
abstract：PURPOSE:To report on isolated central retinal artery occlusion (CRAO) as an initial presentation in two patients with undiagnosed paroxysmal nocturnal hemoglobinuria (PNH). METHODS:CRAO related to the aggravation of PNH was observed in 2 of 98 consecutive PNH patients for 10 years. Ocular and systemic manifestations w...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章,收录出版
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更新日期：2013-09-01 00:00:00
abstract：BACKGROUND:Ophthalmologists should be aware of the signs and symptoms of anthrax, although it is a rare disease in humans. We report our successful treatment of three patients with cutaneous lesions in the periorbital area. CASES:In this study, we report on the treatment of three female patients who were initially dia...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1007/s10384-003-0047-6
更新日期：2004-05-01 00:00:00
abstract：PURPOSE:To clarify the neuronal mechanism for the square-wave jerks (SWJs). METHODS:A 66-year-old man presented with oscillatory eye movements. He showed horizontal nystagmus in rightward gaze and SWJs during fixation at straight ahead. We recorded his eye movements with search coil methods and quantitatively analyzed...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0021-5155(03)00144-8
更新日期：2003-11-01 00:00:00
abstract：PURPOSE:Diabetes mellitus aggravates carotid occlusive disease, that can manifest as ocular ischemic syndrome (OIS). Ocular manifestations and visual prognosis of OIS in diabetic patients were retrospectively analyzed. METHODS:Twenty-three consecutive diabetic patients with OIS were divided into two groups according t...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0021-5155(98)00057-4
更新日期：1999-01-01 00:00:00
abstract：:In an attempt to study the effect of change in the intraocular pressure (IOP) on the status of the vitreous-aqueous interface, the effect of topical bunazosin was studied in the rabbit eye using the new Johnson-Maurice method. This entails an intravitreal injection of FITC-dextran and measurements of its concentration...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:In this report, membranes removed during vitrectomy were observed by various methods of electron microscopy to determine their three-dimensional structure and the mechanism of the contraction of preretinal membranes. The vitreous side surface of the preretinal membranes was covered by two types of flat cells, cells wi...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：PURPOSE:To develop a novel method for measuring tear break-up time (BUT) by continuously measuring higher order wavefront aberrations using a Hartmann-Shack (H-S) aberrometer. METHODS:Wavefront aberrations up to the sixth order for a 4-mm pupil were measured in six eyes of six normal subjects using an H-S aberrometer....
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1007/s10384-005-0289-6
更新日期：2006-03-01 00:00:00
abstract：PURPOSE:To determine the spatial distribution types of macular pigment (MP) in elderly Japanese individuals and to consider their origin. STUDY DESIGN:Observational case series. METHODS:Local MP optical density (MPOD) at some eccentricities and MP volume were measured using the MPOD module of a MultiColor Spectralis ...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1007/s10384-020-00716-5
更新日期：2020-03-01 00:00:00
abstract：:Purpose: Acute retinal necrosis (ARN) is caused by varicella zoster virus (VZV) infection. In this study, we investigated the activity of this virus and expressions of some cytokines.Patients and Methods: The expressionof VZV thymidine kinase and some cytokines were investigated by reverse transcriptase-polymerase cha...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0021-5155(00)00254-9
更新日期：2000-11-01 00:00:00
abstract：PURPOSE:To evaluate the effect of preservative-free (PF) tafluprost on diurnal variation of intraocular pressure (IOP) and ocular perfusion pressure (OPP), measured by use of home IOP and blood-pressure (BP) monitoring devices, for primary open angle glaucoma (POAG) patients. METHODS:Twenty-two eyes from 22 patients w...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1007/s10384-015-0413-1
更新日期：2016-01-01 00:00:00
