Abstract:
:Regulation of fluid flow through corneal stroma was investigated in the bullfrog. Corneal specimens were mounted by clamping their limbal sclera between the two chambers of a Ussing-type chamber. The epithelial surface was covered with Ringer's solution, while the endothelial surface was superfused with Ringer's solution at various pressures ranging from 0-60 mm Hg. At 0 mm Hg, the cornea swelled, while at 10 mm Hg the corneal thickness remained unchanged. Further elevation of the hydrostatic pressure of the endothelial superfusion solution caused a decrease in corneal thickness, suggesting that the hydrostatic pressure in the in vivo frog corneal stroma is about 10 mm Hg. At 10 mm Hg of endothelial superfusion pressure, piercing the epithelial cell layer with a 30-gauge needle caused only slight corneal swelling. Removing glucose from the epithelial perfusion solution induced a slowly progressing increase in corneal thickness. Iodoacetate did not interfere with the swelling of the pierced cornea after the removal of glucose from the epithelial perfusion solution. To examine the possibility that the interstitial fluid flows across the stroma-scleral boundary, corneal specimens having unclamped sclera were incubated in Ringer's solution containing 3 mmol/L dextran of various molecular weights ranging from 8800-162,000, and the volume of the preparation was monitored by sequential measurement of the weight. In the presence of dextran with a molecular weight higher than 70,000, the corneal volume decreased at the beginning of incubation, and after reaching the minimal volume it slowly increased, indicating that the stroma-scleral boundary is permeable to dextran of even a molecular weight of 162,000, although dextran molecules diffuse much more slowly than water, and the concentration of unfilterable solutes in the stroma is lower than 3 mmol/L. In experiments using the Ussing-type chamber at 10 mm Hg of endothelial superfusion pressure, a decrease of NaCl in the superfusing solution to 1/2 caused rapid corneal swelling followed by slow recovery. Adding NaCl to the 1/2 NaCl Ringer's solution caused a further corneal thinning in a concentration-dependent manner. The same extent of decrease in corneal thickness as induced by adding NaCl was achieved by the same concentration of glucose as of NaCl, implying that the value of the reflection coefficient of the endothelial cell layer to either Na+ or Cl- is about half that of glucose. Our results show that even a small difference in the concentration of low molecular weight solutes (e.g., Na+ and Cl-) exerts a force that draws water from the cornea.
journal_name
Jpn J Ophthalmoljournal_title
Japanese journal of ophthalmologyauthors
Kubota S,Kani K,Kitasato Hdoi
10.1016/s0021-5155(97)00111-1subject
Has Abstractpub_date
1998-01-01 00:00:00pages
12-21issue
1eissn
0021-5155issn
1613-2246pii
S0021515597001111journal_volume
42pub_type
杂志文章abstract::This is a clinical and laboratory report on 98 patients with acute viral conjunctivitis who were referred to the Eye Clinic of the Escola Paulista de Medicina of Sao Paulo, Brazil, from February 1983 to February 1984. It was planned with the objective of confirming the importance of adenovirus and enterovirus 70 as et...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1987-01-01 00:00:00
abstract:PURPOSE:To evaluate the degree of ischemia in eyes with retinal vein occlusion (RVO) using swept-source optical coherence tomography angiography (SS-OCTA) with the extended field imaging (EFI) technique, which extends the area encompassed by SS-OCTA by scanning through trial frames fitted with a +20-diopter lens. STUD...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-018-0590-9
更新日期:2018-05-01 00:00:00
abstract::Purpose: We previously reported that a unique free fluorophore (Fl-Glc), presumably a beta-glucoside, is particularly abundant in human brunescent cataractous lens nuclei. Our preliminary experiments indicated that incubation of low-molecular weight (MW) fraction of non-brunescent lens nuclei causes an increase in a p...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0021-5155(00)00236-7
更新日期:2000-09-01 00:00:00
abstract::An animal model was used to evaluate the influence of residual lens cortex on postoperative inflammation and cellular deposits on intraocular lenses (IOLs). Eighteen New Zealand white rabbits underwent bilateral endocapsular phacoemulsification with placement of a one-piece polymethylmethacrylate (PMMA) IOL in the cap...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract:PURPOSE:To elucidate the current clinical practice patterns of branch retinal vein occlusion (BRVO) management by retina specialists in Japan in the era of anti-vascular endothelial growth factor (VEGF) therapy. STUDY DESIGN:A voting survey using an answer pad system. METHODS:On May 28, 2017, forty-one retina special...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章,多中心研究
doi:10.1007/s10384-019-00685-4
更新日期:2019-09-01 00:00:00
abstract:PURPOSE:To evaluate the functional status in daily life and the quality of life (QOL) of pathologic myopia patients. METHODS:A cross-sectional study was conducted using data of consecutive pathologic myopia patients (n = 200) and control subjects (n = 144). The influence of the disease on the daily life and the QOL of...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0021-5155(00)00305-1
更新日期:2001-01-01 00:00:00
abstract:PURPOSE:To present and compare the long-term results of Dr. Tamcelik's previously described technique of Tenon advancement and duplication with the conventional Ahmed glaucoma valve (AGV) implantation technique in patients with refractory glaucoma. METHODS:This study was a multicenter, retrospective case series that i...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1007/s10384-013-0249-5
更新日期:2013-07-01 00:00:00
abstract:PURPOSE:We report here a patient with bilateral papillomacular retinoschisis with an enlarged glaucomatous optic nerve head cup and a focal lamina cribrosa defect, the findings of our clinical investigations of this case, and the chosen treatment and outcome. DESIGN:This is an observational case report. METHODS:Clini...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-014-0330-8
更新日期:2014-09-01 00:00:00
abstract::The spectral sensitivity of the ERG c-wave was studied in the chicken under yellow or red light adaptation of various intensities, ie, 1, 3, 4 and 5 W/m2 at the corneal surface in the former, and 3, 4 and 5 W/m2 in the latter. The peak wavelength of the spectral sensitivity curve of the c-wave amplitude under both yel...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract:BACKGROUND:We report a patient with a limited form of Wegener's granulomatosis (WG) who presented with ocular changes and an eyelid ulcer in her left eye. CASE:A 56-year-old woman complained of ocular pain and discharge. Upon examination, we found evidence of ulcerative keratitis, a mass in the orbit, and an eyelid sk...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0021-5155(03)00078-9
更新日期:2003-07-01 00:00:00
abstract:PURPOSE:To investigate whether retinal glial cells (RGCs), which are believed to play an important role in the development and maintenance of microvessels, stimulate the proliferation of retinal bovine microvascular pericytes, an essential component of the vessels. METHODS:Conditioned medium (CM) was collected from a ...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0021-5155(02)00527-0
更新日期:2002-07-01 00:00:00
abstract:PURPOSE:Defensins are antimicrobial peptides and are classified into three groups, alpha-, beta- and theta-defensins. We investigated the localization of human neutrophil peptides (HNP) 1-3, which are members of the alpha-defensin subfamily, and the expression of human beta-defensin 1 (hBD-1) and hBD-2 in the ocular su...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-004-0163-y
更新日期:2005-03-01 00:00:00
abstract:PURPOSE:A national survey was conducted in Japan to analyze the clinical characteristics of pellucid marginal corneal degeneration (PMD). METHODS:A questionnaire was sent to all members of the Japan Cornea Society requesting demographic and clinical findings on PMD patients who had been examined between 2008 and 2010....
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章,多中心研究
doi:10.1007/s10384-016-0462-0
更新日期:2016-09-01 00:00:00
abstract:PURPOSE:To measure the volume of the anophthalmic orbit in adults who had undergone enucleation during infancy and to determine its growth. METHODS:The orbital volume in 5 adults who had undergone unilateral enucleation during infancy without an orbital implant was measured on x-ray computed tomography images. Compari...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0021-5155(01)00427-0
更新日期:2001-11-01 00:00:00
abstract:PURPOSE:We investigated by in vivo confocal microscopy alterations in the subepithelial nerve plexus in different types of herpes simplex keratitis (HSK). METHODS:Seventeen patients (seven women and ten men, mean age 63.9 years) with a history of HSK were classified into three groups according to the classification of...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-011-0068-5
更新日期:2011-09-01 00:00:00
abstract:PURPOSE:To investigate mutations of causal genes in two affected male siblings of a Japanese family with suspected Leber congenital amaurosis (LCA) and to characterize the related clinical features. METHODS:After obtaining informed consent, genomic DNA was extracted from peripheral blood of the proband and his family ...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-014-0339-z
更新日期:2014-11-01 00:00:00
abstract::The morphological appearance and the quantitative analysis of the structure of the macular microvasculature were investigated in 33 normal subjects by fluorescein angiography and stereoscopic mapping techniques. The foveal capillary network was formed by the anastomosis of 4 to 6 groups of terminal arteriolar and veno...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1985-01-01 00:00:00
abstract::In this report, membranes removed during vitrectomy were observed by various methods of electron microscopy to determine their three-dimensional structure and the mechanism of the contraction of preretinal membranes. The vitreous side surface of the preretinal membranes was covered by two types of flat cells, cells wi...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract:BACKGROUND:We present the pathological findings at orbital exenteration in a patient with Wyburn-Mason syndrome who underwent transarterial embolization. CASE:A 31-year-old man with a 10-year history of gradual exacerbation of left exophthalmos and left cheek swelling was found to have facial and orbital arteriovenous...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-008-0563-5
更新日期:2008-07-01 00:00:00
abstract:PURPOSE:The aim of this study was to experimentally investigate changes in visible light-induced photo-oxidation and to evaluate the inhibitory effect of various acrylic tinted intraocular lenses (IOLs) on photooxidation. METHODS:Three types of nontinted (VA-60BB, HOYA; SA60AT, Alcon; AU-6, Menicon) and tinted (YA-60B...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-008-0620-0
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:Full-thickness macular holes usually develop in the elderly population. To the best of our knowledge, there has been no written report of a nontraumatic macular hole in a pediatric patient. CASE:A 10-year-old girl noticed decreased central vision in her left eye without any history of trauma. OBSERVATIONS:...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-005-0231-y
更新日期:2005-11-01 00:00:00
abstract:PURPOSE:To verify the possibility that preoperative topical antibiotics are not essential as long as iodine disinfection is performed during surgery. STUDY DESIGN:Crossover equivalence trial. PATIENTS AND METHODS:In 204 eyes of 102 patients who underwent routine bilateral cataract surgery, 1 eye was treated with intr...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-019-00703-5
更新日期:2020-01-01 00:00:00
abstract::A 10-year-old girl complained or poor vision in both eyes. The patient showed progeria, physical and mental retardation, sensorineural hearing loss, cutaneous photosensitivity, hyperopia, poor pupillary dilation, exotropia, salt-and-pepper fundi, nondetectable cone and rod electroretinographic (ERG) responses, cerebra...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00
abstract::The effect of pertussis toxin (PT) on experimental autoimmune uveoretinitis (EAU) induced by immunization with S-antigen was examined in rats. Intravenous administration of PT (2 micrograms/rat) initiated the development of EAU in rats that had been made resistant to the induction of EAU by immunization with S-antigen...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract:PURPOSE:The aim of this study was to clarify the demographics, clinical features, and outcomes of Japanese patients with sebaceous carcinoma of the eyelid or conjunctiva. STUDY DESIGN:Retrospective study. METHODS:One hundred twenty-five patients with sebaceous carcinoma of the eyelid or conjunctiva diagnosed at Tokyo...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-020-00756-x
更新日期:2020-09-01 00:00:00
abstract:PURPOSE:To investigate the utility values associated with visual loss due to age-related macular degeneration (AMD) in Japanese patients. METHODS:Utility values in 48 Japanese patients with bilateral exudative AMD were measured using the time trade-off and standard gamble methods. RESULTS:The time trade-off method ut...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-010-0893-y
更新日期:2011-01-01 00:00:00
abstract::The authors experienced one case of malignant melanoma of the superior palpebral conjunctiva and the inferior palpebral skin, originating from benign acquired melanosis of the bulbar conjunctiva which developed in a 48-year-old man. Orbital exenteration including superior and inferior palpebrae was performed, and the ...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1983-01-01 00:00:00
abstract::We investigated the ocular fundus of (NZWXBXSB) F1 mice, which are considered to be a good model for systemic lupus erythematosus (SLE) with antiphospholipid syndrome. The abnormal fundus findings were documented by fundus photography, and the chorioretinal lesions were studied histopathologically. The incidence of oc...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0021-5155(98)00023-9
更新日期:1998-09-01 00:00:00
abstract::The ocular penetration of two beta-adrenergic blockers, befunolol and timolol, was studied using albino and pigmented rabbits. A 1% ophthalmic solution of 14C-befunolol hydrochloride or 14C-timolol maleate was instilled in one eye of nonsedated rabbits, and the concentrations of radioactive material in the ocular tiss...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1982-01-01 00:00:00
abstract:PURPOSE:Lysophosphatidic acid (LPA), which activates the small guanosine triphosphate (GTP)-binding protein Rho, was previously shown to promote migration of the rabbit corneal epithelium in culture. The signaling pathway responsible for this effect of LPA was examined in this study with a human corneal epithelial (HCE...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-003-0059-2
更新日期:2004-05-01 00:00:00