Abstract：
:African swine fever (ASF) is a devastating disease in pigs, with no vaccines for control. The genetic manipulation of African swine fever virus (ASFV) is often tedious and time consuming. Here, we describe a method to manipulate the virus genome to produce gene deletion viruses in a much-reduced time. This method combines the conventional homologous recombination with fluorescent-activated cells sorting (FACS), to isolate and purify viruses expressing fluorescent reporter genes. With three rounds of single cell isolation via FACS and two rounds of limiting dilution, we deleted two additional genes, EP153R and EP402R, from Benin 97/1 ASFV lacking the DP148R gene. By combining different fluorescent markers, this method has the potential to greatly facilitate studies on understanding ASFV gene functions and develop candidate live-attenuated vaccines.
journal_name
Virusesjournal_title
Virusesauthors
Rathakrishnan A,Moffat K,Reis AL,Dixon LKdoi
10.3390/v12060615subject
Has Abstractpub_date
2020-06-05 00:00:00issue
6issn
1999-4915pii
v12060615journal_volume
12pub_type
杂志文章相关文献
Viruses-Basel文献大全abstract：:The hepatitis B virus (HBV) represents a worldwide public health problem; the virus is present in one third of the global population. However, this rate may in fact be higher due to occult hepatitis B virus infection (OBI). This condition is characterized by the presence of the viral genome in the liver of individuals...
journal_title：Viruses
pub_type： 杂志文章,评审
doi：10.3390/v6041590
更新日期：2014-04-08 00:00:00
abstract：:Retroviruses are well known pathogens of mammals, birds and fish. Their potential to induce cancer in chickens was already described almost 100 years ago and murine retroviruses have been a subject of study for 50 years. The first human retroviruses, HTLV and HIV, were discovered more than 30 years ago, surprising res...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v3081312
更新日期：2011-08-01 00:00:00
abstract：:At the end of my doctoral studies, in 1988, I published a review article on the major steps of transcription and translation during the mammalian reovirus multiplication cycle, a topic that still fascinates me 30 years later. It is in the nature of scientific research to generate further questioning as new knowledge e...
journal_title：Viruses
pub_type： 杂志文章,评审
doi：10.3390/v10120671
更新日期：2018-11-27 00:00:00
abstract：:The orthopoxviruses (OPV) comprise several emerging viruses with great importance to human and veterinary medicine, including vaccinia virus (VACV), which causes outbreaks of bovine vaccinia (BV) in South America. Historically, VACV is the most comprehensively studied virus, however, its origin and natural hosts remai...
journal_title：Viruses
pub_type： 杂志文章,评审
doi：10.3390/v9110340
更新日期：2017-11-15 00:00:00
abstract：:Bovine rotavirus A (RVA), a major causative pathogen of diarrhea in dairy and Japanese beef calves, has led to severe economic losses in numerous countries. A dual genotyping system based on genomic segments encoding VP7 (G genotype) and VP4 (P genotype), comprising the outer layer of the virion, has been used to unde...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v12121386
更新日期：2020-12-03 00:00:00
abstract：:Internal ribosome entry is a key mechanism for viral protein synthesis in a subset of RNA viruses. Cricket paralysis virus (CrPV), a member of Dicistroviridae, has a positive-sense single strand RNA genome that contains two internal ribosome entry sites (IRES), a 5'untranslated region (5'UTR) and intergenic region (IG...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v8010025
更新日期：2016-01-19 00:00:00
abstract：:Present in many cell types, non-degradative secretory autophagy is a newly discovered pathway in which autophagosomes fuse with the plasma membrane instead of lysosomes. Surprisingly, some viruses exploit secretory autophagy to exit cells non-lytically, shedding into the extracellular environment as particle populatio...
journal_title：Viruses
pub_type： 杂志文章,评审
doi：10.3390/v10030139
更新日期：2018-03-20 00:00:00
abstract：:Arctic marine ecosystems are currently undergoing rapid changes in temperature and light availability. Picophytoplankton, such as Micromonas polaris, are predicted to benefit from such changes. However, little is known about how these environmental changes affect the viruses that exert a strong mortality pressure on t...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v10120676
更新日期：2018-11-29 00:00:00
abstract：:SARS-CoV-2 enters cells using its Spike protein, which is also the main target of neutralizing antibodies. Therefore, assays to measure how antibodies and sera affect Spike-mediated viral infection are important for studying immunity. Because SARS-CoV-2 is a biosafety-level-3 virus, one way to simplify such assays is ...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v12050513
更新日期：2020-05-06 00:00:00
abstract：:The Spounavirinae viruses are ubiquitous in nature and have an obligatory virulent lifestyle. They infect Firmicutes, a bacterial phylum containing an array of environmental non-pathogenic and pathogenic organisms. To expand the knowledge of this viral subfamily, new strains were isolated and investigated in this stud...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v9060146
更新日期：2017-06-12 00:00:00
abstract：:The human cytomegalovirus (HCMV)-encoded cyclin-dependent kinase (CDK) ortholog pUL97 associates with human cyclin B1 and other types of cyclins. Here, the question was addressed whether cyclin interaction of pUL97 and additional viral proteins is detectable by mass spectrometry-based approaches. Proteomic data were v...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v8080219
更新日期：2016-08-18 00:00:00
abstract：:Laboratories throughout the world that perform work with Risk Group 4 Pathogens generally adopt one of two approaches within BSL-4 environments: either the use of positive pressure air-fed suits or using Class III microbiological safety cabinets and isolators for animal work. Within the UK at present, all laboratories...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v4081202
更新日期：2012-08-01 00:00:00
abstract：:Salmonid alphavirus (SAV) is the cause of pancreas disease and sleeping disease in farmed salmonid fish in Europe. The spread of these diseases has been difficult to control with biosecurity and current vaccination strategies, and increased understanding of the viral pathogenesis could be beneficial for the developmen...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v12101071
更新日期：2020-09-24 00:00:00
abstract：:Several notable human diseases are caused by enveloped RNA viruses: influenza, AIDS, hepatitis C, dengue hemorrhagic fever, microcephaly, and Guillain-Barré Syndrome. Being enveloped, the life cycle of this group of viruses is critically dependent on host lipid biosynthesis. Viral binding and entry involve interaction...
journal_title：Viruses
pub_type： 杂志文章,评审
doi：10.3390/v11100912
更新日期：2019-10-01 00:00:00
abstract：:Nervous necrosis virus (NNV) can infect many species of fish and has an 80-100% mortality rate. NNV capsid protein (NNVCP) is the only structural protein of NNV, but there are few studies on the protein-protein interaction between NNVCP and the host cell. To investigate NNV morphogenesis, native NNV capsid protein (NN...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v12090985
更新日期：2020-09-04 00:00:00
abstract：:Human T-cell leukemia virus type 1 (HTLV-1) is a retrovirus that causes cancer (Adult T cell Leukemia, ATL) and a spectrum of inflammatory diseases (mainly HTLV-associated myelopathy-tropical spastic paraparesis, HAM/TSP). Since virions are particularly unstable, HTLV-1 transmission primarily occurs by transfer of a c...
journal_title：Viruses
pub_type： 杂志文章,评审
doi：10.3390/v7072793
更新日期：2015-07-07 00:00:00
abstract：:Although there have been many epidemiological reports of the inter-country transmission of measles, systematic analysis of the global transmission dynamics of the measles virus (MV) is limited. In this study, we applied phylogeographic analysis to characterize the global transmission dynamics of the MV using large-sca...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v9040082
更新日期：2017-04-16 00:00:00
abstract：:Significantly higher numbers of human infections with H5N1 virus have occurred in Indonesia and Egypt, compared with other affected areas, and it is speculated that there are specific viral factors for human infection with avian H5N1 viruses in these locations. We previously showed PB2-K526R is present in 80% of Indon...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v11030292
更新日期：2019-03-22 00:00:00
abstract：:Herpesviruses use a vesicle-mediated transfer of intranuclearly assembled nucleocapsids through the nuclear envelope (NE) for final maturation in the cytoplasm. The molecular basis for this novel vesicular nucleo-cytoplasmic transport is beginning to be elucidated in detail. The heterodimeric viral nuclear egress comp...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v8100266
更新日期：2016-09-27 00:00:00
abstract：:Type-I interferons (IFN-I) play an important role in the innate immune response to several retroviruses. They seem to be effective in controlling the in vivo infection, though many of the clinical signs of retroviral infection may be due to their continual presence which over-stimulates the immune system and activates...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v1030545
更新日期：2009-12-01 00:00:00
abstract：:Hepatitis B surface antigen (HBsAg) promotes persistent hepatitis B virus (HBV) infection. It primarily corresponds to small (S) envelope protein secreted as subviral particles. We previously found that genotype D clones expressed less S protein than genotype A clones but showed higher extracellular/intracellular rati...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v12090967
更新日期：2020-09-01 00:00:00
abstract：:Janus kinase (JAK) inhibitors have been developed as novel immunomodulatory drugs and primarily used for treating rheumatoid arthritis and other inflammatory diseases. Recent studies have suggested that this category of anti-inflammatory drugs could be potentially useful for the control of inflammation "storms" in res...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v12101141
更新日期：2020-10-08 00:00:00
abstract：:Avian infectious bronchitis has caused huge economic losses in the poultry industry. Previous studies have reported that infectious bronchitis virus (IBV) infection can produce cytopathic effects (CPE) and apoptosis in some mammalian cells and primary cells. However, there is little research on IBV-induced immune cell...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v9080198
更新日期：2017-07-26 00:00:00
abstract：:Victoria and Yamagata lineages of influenza B viruses are globally circulating in seasonal epidemics. Madin-Darby canine kidney (MDCK) cells are permissive for viral isolation and vaccine manufacture. Nevertheless, the interplay between influenza B viruses and host microRNAs has not been investigated in this cell line...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v11110986
更新日期：2019-10-25 00:00:00
abstract：:Alfuy (ALFV) is an attenuated flavivirus related to the Murray Valley encephalitis virus (MVEV). We previously identified markers of attenuation in the envelope (E) protein of the prototype strain (ALFV3929), including the hinge region (E273-277) and lack of glycosylation at E154-156. To further determine the mechanis...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v13020147
更新日期：2021-01-20 00:00:00
abstract：:Finding a cure for HIV is challenging because the virus is able to integrate itself into the host cell genome and establish a silent state, called latency, allowing it to evade antiviral drugs and the immune system. Various "shock and kill" strategies are being explored in attempts to eliminate latent HIV reservoirs. ...
journal_title：Viruses
pub_type： 杂志文章,评审
doi：10.3390/v10120677
更新日期：2018-11-30 00:00:00
abstract：:Influenza is a serious public health concern worldwide, as it causes significant morbidity and mortality. The emergence of drug-resistant viral strains requires new approaches for the treatment of influenza. In this study, Rubus coreanus seed (RCS) that is left over from the production of wine or juice was found to sh...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v8060157
更新日期：2016-06-06 00:00:00
abstract：:Emerging evidence highlights a critical role for protein acetylation during herpesvirus infection. As prominent modulators of protein acetylation, histone deacetylases (HDACs) are essential transcriptional and epigenetic regulators. Not surprisingly, viruses have evolved a wide array of mechanisms to subvert HDAC func...
journal_title：Viruses
pub_type： 杂志文章,评审
doi：10.3390/v5071607
更新日期：2013-06-27 00:00:00
abstract：:How microbial populations interact influences the availability and flux of organic carbon in the ocean. Understanding how these interactions vary over broad spatial scales is therefore a fundamental aim of microbial oceanography. In this study, we assessed variations in the abundances, production, virus and grazing in...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v12111293
更新日期：2020-11-12 00:00:00
abstract：:Human T-cell leukemia virus type 1 (HTLV-1) is an important cancer-causing human retrovirus that has infected approximately 15 million individuals worldwide. Many aspects of HTLV-1 replication, including virus particle structure and assembly, are poorly understood. Group-specific antigen (Gag) proteins labeled at the ...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v9070191
更新日期：2017-07-19 00:00:00
