Abstract：
:Hepatitis B surface antigen (HBsAg) promotes persistent hepatitis B virus (HBV) infection. It primarily corresponds to small (S) envelope protein secreted as subviral particles. We previously found that genotype D clones expressed less S protein than genotype A clones but showed higher extracellular/intracellular ratio of HBsAg suggesting more efficient secretion. The current study aimed to characterize the underlying mechanism(s) by comparing a subgenotype A2 clone (geno5.4) with a subgenotype D2 clone (geno1.2). Five types of full-length or subgenomic constructs were transfected to Huh7 cells at different dosage. HBsAg was quantified by enzyme linked immunosorbent assay while envelope proteins were detected by Western blot. We found that ratio of extracellular/intracellular HBsAg decreased at increasing amounts of DNA transfected. Conflicting findings from two types of subgenomic construct confirmed stronger secretion inhibitory effect of the genotype D-derived large envelope protein. Chimeric constructs followed by site-directed mutagenesis revealed geno1.2 specific V118/T127 and F161/A168 in the S protein as promoting and inhibitory of HBsAg secretion, respectively. In conclusion, more efficient HBsAg secretion by subgenotype D2 than subgenotype A2 is attributed to lower level of S protein expression in addition to V118 and T127 in S protein, although its F161 and A168 sequences rather reduce HBsAg secretion.
journal_name
Virusesjournal_title
Virusesauthors
Wang Q,Fu S,Zhang J,Yuan Q,Li J,Xia N,Wen YM,Wang Y,Tong Sdoi
10.3390/v12090967subject
Has Abstractpub_date
2020-09-01 00:00:00issue
9issn
1999-4915pii
v12090967journal_volume
12pub_type
杂志文章相关文献
Viruses-Basel文献大全abstract：:Hantaviruses, members of the order Bunyavirales, family Hantaviridae, have a world-wide distribution and are responsible for greater than 150,000 cases of disease per year. The spectrum of disease associated with hantavirus infection include hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrom...
journal_title：Viruses
pub_type： 杂志文章,评审
doi：10.3390/v11070610
更新日期：2019-07-04 00:00:00
abstract：:The clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 system is widely explored for sequence-specific attack on HIV-1 proviral DNA. We recently identified dual-guide RNA (dual-gRNA) combinations that can block HIV-1 replication permanently in infected cell cultures and prevent viral escape. Altho...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v11030255
更新日期：2019-03-13 00:00:00
abstract：:Since the human immunodeficiency virus (HIV) was discovered as the etiological agent of acquired immunodeficiency syndrome (AIDS), it has encouraged much research into antiviral compounds. The reverse transcriptase (RT) of HIV has been a main target for antiviral drugs. However, all drugs developed so far inhibit the ...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v2040900
更新日期：2010-04-01 00:00:00
abstract：:Micro RNAs (miRNAs) are a class of small non-coding single-stranded RNA, which play an important role in modulating host-Influenza A virus (IAV) crosstalk. The interplay between influenza and miRNA interaction is defined by a plethora of complex mechanisms, which are not fully understood yet. Here, we demonstrate that...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v12040444
更新日期：2020-04-15 00:00:00
abstract：:Fruit trees, such as apricot trees, are constantly exposed to the attack of viruses. As they are propagated in a vegetative way, this risk is present not only in the field, where they remain for decades, but also during their propagation. Metagenomic diagnostic methods, based on next generation sequencing (NGS), offer...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v10060318
更新日期：2018-06-11 00:00:00
abstract：:Next-generation sequencing (NGS) has opened up the possibility of detecting new viruses in unresolved diseases. Recently, astrovirus brain infections have been identified in neurologically diseased humans and animals by NGS, among them bovine astrovirus (BoAstV) CH13/NeuroS1, which has been found in brain tissues of c...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v9010012
更新日期：2017-01-18 00:00:00
abstract：:It is difficult to observe the molecular choreography between viruses and host cell components, as they exist on a spatial scale beyond the reach of conventional microscopy. However, novel super-resolution microscopy techniques have cast aside technical limitations to reveal a nanoscale view of virus replication and c...
journal_title：Viruses
pub_type： 杂志文章,评审
doi：10.3390/v6031365
更新日期：2014-03-19 00:00:00
abstract：:Merkel cell polyomavirus (MCV) is the only known human oncogenic virus in the polyomaviridae family and the etiological agent of most Merkel cell carcinomas (MCC). MCC is an aggressive and highly metastatic skin cancer with a propensity for recurrence and poor prognosis. Large tumor antigen (LT), is an essential oncop...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v12091043
更新日期：2020-09-18 00:00:00
abstract：:Human T-cell leukemia virus type 1 (HTLV-1) is an important cancer-causing human retrovirus that has infected approximately 15 million individuals worldwide. Many aspects of HTLV-1 replication, including virus particle structure and assembly, are poorly understood. Group-specific antigen (Gag) proteins labeled at the ...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v9070191
更新日期：2017-07-19 00:00:00
abstract：:Extensive surveillance in bat populations in response to recent emerging diseases has revealed that this group of mammals acts as a reservoir for a large range of viruses. However, the oldest known association between a zoonotic virus and a bat is that between rabies virus and the vampire bat. Vampire bats are only fo...
journal_title：Viruses
pub_type： 杂志文章,评审
doi：10.3390/v6051911
更新日期：2014-04-29 00:00:00
abstract：:Dengue virus (DENV) infection has become a critically important globally prevalent infectious disease, especially in tropical and subtropical countries. Since neither currently exists, there is an urgent need for an effective vaccine to prevent, and a specific drug to treat DENV infection. Therapeutic peptides represe...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v12111267
更新日期：2020-11-06 00:00:00
abstract：:Bluetongue (BT) is an arthropod-borne viral disease of ruminants with serious trade and socio-economic implications. Although the disease has been reported in a number of countries in sub-Saharan Africa, there is currently no information on circulating serotypes and disease distribution in Zambia. Following surveillan...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v12090963
更新日期：2020-08-31 00:00:00
abstract：:Timely implementation of antiviral treatment and other public health based responses are dependent on accurate and rapid diagnosis of the novel pandemic influenza A(H1N1) strain. In this study we developed a duplex real-time PCR (RT-PCR) (dFLU-TM) assay for the simultaneous detection of a broad range of influenza A su...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v1031204
更新日期：2009-12-01 00:00:00
abstract：:Deoxynucleoside triphosphate (dNTP) molecules are essential for the replication and maintenance of genomic information in both cells and a variety of viral pathogens. While the process of dNTP biosynthesis by cellular enzymes, such as ribonucleotide reductase (RNR) and thymidine kinase (TK), has been extensively inves...
journal_title：Viruses
pub_type： 杂志文章,评审
doi：10.3390/v12040382
更新日期：2020-03-31 00:00:00
abstract：:The porcine epidemic diarrhea virus (PEDV) poses a great threat to the global swine industries and the unreliable protection induced by the currently available vaccines remains a major challenge. We previously generated a genogroup 2b (G2b) PEDV Taiwan Pintung 52 (PEDVPT) strain, PEDVPT-P96, and determined its promisi...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v10100543
更新日期：2018-10-04 00:00:00
abstract：:Influenza A virus is a successful parasite and requires host factors to complete its life cycle. Prop5 is an antisense oligonucleotide, targeting programmed cell death protein 5 (PDCD5). In this study, we tested the antiviral activity of prop5 against mouse-adapted A/FM/1/47 strain of influenza A virus in a mouse mode...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v6020573
更新日期：2014-02-06 00:00:00
abstract：:Bacteriophage (phage) therapy is a promising alternative to antibiotics for the treatment of bacterial pathogens, including Clostridiumdifficile. However, as for many species, in C. difficile the physical interactions between phages and bacterial cells have not been studied in detail. The initial interaction, known as...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v10080411
更新日期：2018-08-06 00:00:00
abstract：:JC Virus (JCPyV), a member of the Polyomaviridiæ family, is a human neurotropic virus with world-wide distribution. JCPyV is the established opportunistic infectious agent of progressive multifocal leukoencephalopathy, a fatal demyelinating disease, which results from the cytolytic infection of oligodendrocytes. Mutat...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v13020162
更新日期：2021-01-22 00:00:00
abstract：:Campylobacter jejuni is a frequent foodborne pathogen of humans. As C. jejuni infections commonly arise from contaminated poultry, phage treatments have been proposed to reduce the C. jejuni load on farms to prevent human infections. While a prior report documented the transcriptome of C. jejuni phages during the carr...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v10060332
更新日期：2018-06-16 00:00:00
abstract：:Bacterial infections pose a challenge to human health and burden the health care system, especially with the spread of antibiotic-resistant populations. To provide effective treatment and improved prognosis, effective diagnostic methods are of great importance. Here we present phage-mediated molecular detection (PMMD)...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v12040435
更新日期：2020-04-11 00:00:00
abstract：:Bacteriophages represent a valuable source for studying the mechanisms underlying virus-host interactions. A better understanding of the host-specificity of viruses at the molecular level can promote various phage applications, including bacterial diagnostics, antimicrobial therapeutics, and improve methods in molecul...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v7122932
更新日期：2015-11-27 00:00:00
abstract：:Behçet's disease (BD) affects multiple organs. It is mainly characterized by recurrent oral, skin, and genital aphthous ulcers, and eye involvement. Successful management of BD is increasing, although its etiology remains unclear. A number of etiologies have been proposed, including environmental, genetic, viral, and ...
journal_title：Viruses
pub_type： 杂志文章,评审
doi：10.3390/v10090511
更新日期：2018-09-19 00:00:00
abstract：:A metagenomic study was performed on 498 female and 40 male Aedes albopictus mosquitos collected in August and September 2019 in Ticino, a region in southern Switzerland, to address the question regarding the risk of the local transmission of zoonotic viruses. A total of 13 viruses from seven different virus families ...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v12090929
更新日期：2020-08-24 00:00:00
abstract：:The HIV-1 Gag p6 protein regulates the final abscission step of nascent virions from the cell membrane by the action of its two late (L-) domains, which recruit Tsg101 and ALIX, components of the ESCRT system. Even though p6 consists of only 52 amino acids, it is encoded by one of the most polymorphic regions of the H...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v8040117
更新日期：2016-04-25 00:00:00
abstract：:Bovine viral diarrhea virus (BVDV) is a globally-distributed agent responsible for numerous clinical syndromes that lead to major economic losses. Two species, BVDV-1 and BVDV-2, discriminated on the basis of genetic and antigenic differences, are classified in the genus Pestivirus within the Flaviviridae family and d...
journal_title：Viruses
pub_type： 杂志文章,评审
doi：10.3390/v9060128
更新日期：2017-05-26 00:00:00
abstract：:Tetraspanins are integral transmembrane proteins organized in microdomains displaying specific and direct interactions with other tetraspanins and molecular partners. Among them, CD81 has been implicated in a variety of physiological and pathological processes. CD81 also plays a crucial role in pathogen entry into hos...
journal_title：Viruses
pub_type： 杂志文章,评审
doi：10.3390/v6020875
更新日期：2014-02-18 00:00:00
abstract：:Porcine Reproductive and Respiratory Syndrome (PRRS) is a contagious viral (PRRSV) disease in pigs characterized by poor reproductive health, increased mortality, and reductions in growth rates. PRRSV is known to implement immuno-antagonistic mechanisms to evade detection and mute host responses to infection. To bette...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v12111299
更新日期：2020-11-12 00:00:00
abstract：:Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) systems are key immune mechanisms helping prokaryotic species fend off RNA and DNA viruses. CRISPR/Cas9 has broad applications in basic research and biotechnology and has been widely used across eukaryotic species for genome...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v10120732
更新日期：2018-12-19 00:00:00
abstract：:Ebola virus (EBOV) is a highly lethal pathogen that has caused several outbreaks of severe hemorrhagic fever in humans since its emergence in 1976. The EBOV glycoprotein (GP1,2) is the sole viral envelope protein and a major component of immunogenicity; it is encoded by the GP gene along with two truncated versions: s...
journal_title：Viruses
pub_type： 杂志文章,评审
doi：10.3390/v11110999
更新日期：2019-10-31 00:00:00
abstract：:Food security is currently a global socio-political pressure point that is exacerbated by the everincreasing world population[...]. ...
journal_title：Viruses
pub_type： 社论
doi：10.3390/v12020210
更新日期：2020-02-13 00:00:00
