Abstract:
:Porcine Reproductive and Respiratory Syndrome (PRRS) is a contagious viral (PRRSV) disease in pigs characterized by poor reproductive health, increased mortality, and reductions in growth rates. PRRSV is known to implement immuno-antagonistic mechanisms to evade detection and mute host responses to infection. To better understand the cellular immunosignature of PRRSV we have undertaken transcriptome and immunomodulatory studies in PRRSV-infected porcine alveolar macrophages (PAMs). We first used genome-wide transcriptome profiling (RNA-seq) to elucidate PRRSV-induced changes in the PAM transcriptome in response to infection. We found a number of cellular networks were altered by PRRSV infection, including many associated with innate immunity, such as, the NLRP3 inflammasome. To further explore the role(s) of innate immune networks in PRRSV-infected PAMs, we used an NLRP3-specific inhibitor, MCC950, to identify the potential functionality of the inflammasome during PRRSV replication. We found that PRRSV does quickly induce expression of inflammasome-associated genes in PAMs. Treatment of PAMs with MCC950 suggests NLRP3 inflammasome activation negatively impacts viral replication. Treatment of PAMs with cell culture supernatants from macrophages subjected to NLRP3 inflammasome activation (via polyinosinic-polycytidylic acid (poly I:C) transfection), prior to PRRSV infection resulted in significantly reduced viral RNA levels compared to PAMs treated with cell culture supernatants from macrophages subjected to NLRP3 inflammasome inhibition (MCC950 treatment/poly I:C transfection). This further supports a role for NLRP3 inflammasome activation in the innate macrophagic anti-PRRSV immune response and suggests that PRRSV is sensitive to the effects of NLRP3 inflammasome activity. Taken together, these transcriptome and immunoregulatory data highlight the complex changes PRRSV infection induces in the molecular immune networks of its cellular host.
journal_name
Virusesjournal_title
Virusesauthors
Hicks JA,Yoo D,Liu HCdoi
10.3390/v12111299subject
Has Abstractpub_date
2020-11-12 00:00:00issue
11issn
1999-4915pii
v12111299journal_volume
12pub_type
杂志文章相关文献
Viruses-Basel文献大全abstract::Lactococcus lactis is one of the most important bacteria in dairy fermentations, being used in the production of cheese and buttermilk. The processes are vulnerable to phage attacks, and undefined mixtures of lactococcal strains are often used to reduce the risk of bacteriophage caused fermentation failure. Other prev...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v11050443
更新日期:2019-05-16 00:00:00
abstract::Virus-induced inflammation plays a critical role in determining the clinical outcome of an acute respiratory virus infection. We have shown previously that the administration of immunobiotic Lactobacillus plantarum (Lp) directly to the respiratory tract prevents lethal inflammatory responses to subsequent infection wi...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v13010002
更新日期:2020-12-22 00:00:00
abstract::A myriad of factors favor the emergence and re-emergence of arthropod-borne viruses (arboviruses), including migration, climate change, intensified livestock production, an increasing volume of international trade and transportation, and changes to ecosystems (e.g., deforestation and loss of biodiversity). Consequentl...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v3060629
更新日期:2011-06-01 00:00:00
abstract::Double-stranded DNA viruses package their genomes into pre-assembled protein procapsids. This process is driven by macromolecular motors that transiently assemble at a unique vertex of the procapsid and utilize homomeric ring ATPases to couple genome encapsidation to ATP hydrolysis. Here, we describe the biochemical a...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v13010015
更新日期:2020-12-23 00:00:00
abstract::Severe fever with thrombocytopenia syndrome (SFTS), a tick-borne infectious disease, is difficult to differentiate from other common febrile diseases. Clinically distinctive features and climate variates associated with tick growth can be useful predictors for SFTS. This retrospective study (2013-2019) demonstrated th...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v13010010
更新日期:2020-12-23 00:00:00
abstract::At the end of my doctoral studies, in 1988, I published a review article on the major steps of transcription and translation during the mammalian reovirus multiplication cycle, a topic that still fascinates me 30 years later. It is in the nature of scientific research to generate further questioning as new knowledge e...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v10120671
更新日期:2018-11-27 00:00:00
abstract::Human respiratory syncytial virus (HRSV) is a major cause of pediatric infection and also causes disease in the elderly and those with underlying respiratory problems. There is no vaccine for HRSV and anti-viral therapeutics are not broadly applicable. To investigate the effect of HRSV biology in children, nasopharyng...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v11100926
更新日期:2019-10-10 00:00:00
abstract::Mechanistic models are critical for our understanding of both within-host dynamics (i.e., pathogen replication and immune system processes) and among-host dynamics (i.e., transmission). Within-host models, however, are not often fit to experimental data, which can serve as a robust method of hypothesis testing and hyp...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v11050396
更新日期:2019-04-27 00:00:00
abstract::Rabies is a viral infection that targets the nervous system, specifically neurons. The clinical manifestations of the disease are dramatic and their outcome fatal; paradoxically, conventional histopathological descriptions reveal only subtle changes in the affected nervous tissue. Some researchers have considered that...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v10030112
更新日期:2018-03-06 00:00:00
abstract::Emergence of human immunodeficiency virus type 1 (HIV-1) drug resistance arises from mutation fixation in the viral genome during antiretroviral therapy. Primary mutations directly confer antiviral drug resistance, while secondary mutations arise that do not confer drug resistance. The A62V amino acid substitution in ...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v10070376
更新日期:2018-07-19 00:00:00
abstract::Although there have been many epidemiological reports of the inter-country transmission of measles, systematic analysis of the global transmission dynamics of the measles virus (MV) is limited. In this study, we applied phylogeographic analysis to characterize the global transmission dynamics of the MV using large-sca...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v9040082
更新日期:2017-04-16 00:00:00
abstract::Peptides derived from the C-terminal heptad repeat (CHR) region of HIV-1 gp41 is potent viral membrane fusion inhibitors, such as the first clinically approved peptide drug T20 and a group of newly-designed peptides. The resistance profiles of various HIV-1 fusion inhibitors were previously characterized, and the seco...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12030326
更新日期:2020-03-18 00:00:00
abstract::Monkeypox is a smallpox-like illness that can be accompanied by a range of significant medical complications. To date there are no standard or optimized guidelines for the clinical management of monkeypox (MPX) patients, particularly in low-resource settings. Consequently, patients can experience protracted illness an...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v9120380
更新日期:2017-12-12 00:00:00
abstract::West Nile (WNV) and Dengue (DENV) viruses are major arboviral human pathogens belonging to the genus Flavivirus. At the current time, there are no approved prophylactics (e.g., vaccines) or specific therapeutics available to prevent or treat human infections by these pathogens. Due to their minimal genome, these virus...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v6020683
更新日期:2014-02-10 00:00:00
abstract::A lytic bacteriophage PHB01 specific for Pasteurella multocida type D was isolated from the sewage water collected from a pig farm. This phage had the typical morphology of the family Podoviridae, order Caudovirales, presenting an isometric polyhedral head and a short noncontractile tail. PHB01 was able to infect most...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v11010086
更新日期:2019-01-21 00:00:00
abstract::Recently, Alves-Freitas and colleagues [...]. ...
journal_title:Viruses
pub_type: 评论,信件
doi:10.3390/v11030230
更新日期:2019-03-07 00:00:00
abstract::Monoderm bacteria possess a cell envelope made of a cytoplasmic membrane and a cell wall, whereas diderm bacteria have and extra lipid layer, the outer membrane, covering the cell wall. Both cell types can also produce extracellular protective coats composed of polymeric substances like, for example, polysaccharidic c...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v10080396
更新日期:2018-07-27 00:00:00
abstract::Herpesviruses use a vesicle-mediated transfer of intranuclearly assembled nucleocapsids through the nuclear envelope (NE) for final maturation in the cytoplasm. The molecular basis for this novel vesicular nucleo-cytoplasmic transport is beginning to be elucidated in detail. The heterodimeric viral nuclear egress comp...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v8100266
更新日期:2016-09-27 00:00:00
abstract::Prymnesium parvum is a toxin-producing haptophyte that causes harmful algal blooms globally, leading to large-scale fish kills that have severe ecological and economic implications. For the model haptophyte, Emiliania huxleyi, it has been shown that large dsDNA viruses play an important role in regulating blooms and t...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v9030040
更新日期:2017-03-09 00:00:00
abstract::Persistent infection by human papillomaviruses (HPVs), small, double-stranded DNA viruses that infect keratinocytes of the squamous epithelia, can lead to the development of cervical and other cancers. The viral oncoprotein E7 contributes to viral persistence in part by regulating host gene expression through binding ...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12030311
更新日期:2020-03-13 00:00:00
abstract::We recently developed a test based on the Agilent SureSelect target enrichment system capturing genomic fragments from 191 human papillomaviruses (HPV) types for Illumina sequencing. This enriched whole genome sequencing (eWGS) assay provides an approach to identify all HPV types in a sample. Here we present a machine...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12070710
更新日期:2020-06-30 00:00:00
abstract::Merkel cell polyomavirus (MCV) is the only known human oncogenic virus in the polyomaviridae family and the etiological agent of most Merkel cell carcinomas (MCC). MCC is an aggressive and highly metastatic skin cancer with a propensity for recurrence and poor prognosis. Large tumor antigen (LT), is an essential oncop...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12091043
更新日期:2020-09-18 00:00:00
abstract::Retroviruses integrate a reverse transcribed double stranded DNA copy of their viral genome into the chromosomal DNA of cells they infect. Occasionally, exogenous retroviruses infect germ cells and when this happens a profound shift in the virus host dynamic occurs. Retroviruses maintained as hereditable viral genetic...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v3060901
更新日期:2011-06-01 00:00:00
abstract::Around 257 million people are living with hepatitis B virus (HBV) chronic infection and 71 million with hepatitis C virus (HCV) chronic infection. Both HBV and HCV infections can lead to liver complications such as cirrhosis and hepatocellular carcinoma (HCC). To take care of these chronically infected patients, one s...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v12121440
更新日期:2020-12-14 00:00:00
abstract::Andes orthohantavirus (ANDV) is an important human pathogen causing hantavirus cardiopulmonary syndrome (HCPS) with a fatality rate of 30% in Chile. Around 60% of all cases have a severe clinical course, while the others have a mild clinical course. The main goal of this study was to understand if the genetic variatio...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v11080680
更新日期:2019-07-25 00:00:00
abstract::Efforts towards developing a vaccine for Middle East respiratory syndrome coronavirus (MERS-CoV) have yielded promising results. Utilizing a variety of platforms, several vaccine approaches have shown efficacy in animal models and begun to enter clinical trials. In this review, we summarize the current progress toward...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v11010074
更新日期:2019-01-17 00:00:00
abstract::Dengue virus (DENV) infection has become a critically important globally prevalent infectious disease, especially in tropical and subtropical countries. Since neither currently exists, there is an urgent need for an effective vaccine to prevent, and a specific drug to treat DENV infection. Therapeutic peptides represe...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12111267
更新日期:2020-11-06 00:00:00
abstract::Arthropod-borne flaviviruses are human pathogens of global medical importance, against which no effective small molecule-based antiviral therapy has currently been reported. Arbidol (umifenovir) is a broad-spectrum antiviral compound approved in Russia and China for prophylaxis and treatment of influenza. This compoun...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v10040184
更新日期:2018-04-10 00:00:00
abstract::The honey bee queen is the central hub of a colony to produce eggs and release pheromones to maintain social cohesion. Among many environmental stresses, viruses are a major concern to compromise the queen's health and reproductive vigor. Viruses have evolved numerous strategies to infect queens either via vertical tr...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v12030322
更新日期:2020-03-17 00:00:00
abstract::RNA secondary structures play diverse roles in positive-sense (+) RNA virus infections, but those located with the replication protein coding sequence can be difficult to investigate. Structures that regulate the translation of replication proteins pose particular challenges, as their potential involvement in post-tra...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12030350
更新日期:2020-03-22 00:00:00