Abstract:
:Peptides derived from the C-terminal heptad repeat (CHR) region of HIV-1 gp41 is potent viral membrane fusion inhibitors, such as the first clinically approved peptide drug T20 and a group of newly-designed peptides. The resistance profiles of various HIV-1 fusion inhibitors were previously characterized, and the secondary mutation N126K in the gp41 CHR was routinely identified during the in vitro and in vivo selections. In this study, the functional and structural relevance of the N126K mutation has been characterized from multiple angles. First, we show that a single N126K mutation across several HIV-1 isolates conferred mild to moderate cross-resistances. Second, the N126K mutation exerted different effects on Env-mediated HIV-1 entry and cell-cell fusion. Third, the N126K mutation did not interfere with the expression and processing of viral Env glycoproteins, but it disrupted the Asn126-based glycosylation site in gp41. Fourth, the N126K mutation was verified to enhance the thermal stability of 6-HB conformation. Fifth, we determined the crystal structure of a 6-HB bearing the N126K mutation, which revealed the interhelical and intrahelical interactions underlying the increased thermostability. Therefore, our data provide new information to understand the mechanism of HIV-1 gp41-mediated cell fusion and its resistance mode to viral fusion inhibitors.
journal_name
Virusesjournal_title
Virusesauthors
Yu D,Su Y,Ding X,Zhu Y,Qin B,Chong H,Cui S,He Ydoi
10.3390/v12030326subject
Has Abstractpub_date
2020-03-18 00:00:00issue
3issn
1999-4915pii
v12030326journal_volume
12pub_type
杂志文章相关文献
Viruses-Basel文献大全abstract::Low pathogenic avian influenza (LPAI) H7N9 viruses have recently evolved to gain a polybasic cleavage site in the hemagglutinin (HA) protein, resulting in variants with increased lethality in poultry that meet the criteria for highly pathogenic avian influenza (HPAI) viruses. Both LPAI and HPAI variants can cause seve...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12010065
更新日期:2020-01-05 00:00:00
abstract::Flaviviruses, most of which are emerging and re-emerging human pathogens and significant public health concerns worldwide, are positive-sense RNA viruses. Flavivirus replication occurs on the ER and is regulated by many mechanisms and factors. NS5, which consists of a C-terminal RdRp domain and an N-terminal methyltra...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v11100929
更新日期:2019-10-10 00:00:00
abstract::Different lines of evidence support an association between Epstein-Barr virus (EBV) and gastric cancer (GC). The main understood risk factor to develop GC is infection by Helicobacter pylori (H. pylori), which triggers a local inflammatory response critical for progression from gastritis to GC. The role of EBV in earl...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v6010301
更新日期:2014-01-20 00:00:00
abstract::Human respiratory syncytial virus (HRSV) is a major cause of pediatric infection and also causes disease in the elderly and those with underlying respiratory problems. There is no vaccine for HRSV and anti-viral therapeutics are not broadly applicable. To investigate the effect of HRSV biology in children, nasopharyng...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v11100926
更新日期:2019-10-10 00:00:00
abstract::Bovine rotavirus A (RVA), a major causative pathogen of diarrhea in dairy and Japanese beef calves, has led to severe economic losses in numerous countries. A dual genotyping system based on genomic segments encoding VP7 (G genotype) and VP4 (P genotype), comprising the outer layer of the virion, has been used to unde...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12121386
更新日期:2020-12-03 00:00:00
abstract::Human enteroviruses (HEV), especially coxsackievirus serotype B (CVB) and echovirus (E), have been associated with diseases of both the exocrine and endocrine pancreas, but so far evidence on HEV infection in human pancreas has been reported only in islets and ductal cells. This study aimed to investigate the capabili...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v9020025
更新日期:2017-01-30 00:00:00
abstract::An efficacious HIV vaccine is urgently needed to curb the AIDS pandemic. The modest protection elicited in the phase III clinical vaccine trial in Thailand provided hope that this goal might be achieved. However, new approaches are necessary for further advances. As HIV is transmitted primarily across mucosal surfaces...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v6083129
更新日期:2014-08-15 00:00:00
abstract::Type-I interferons (IFN-I) play an important role in the innate immune response to several retroviruses. They seem to be effective in controlling the in vivo infection, though many of the clinical signs of retroviral infection may be due to their continual presence which over-stimulates the immune system and activates...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v1030545
更新日期:2009-12-01 00:00:00
abstract::While dromedaries are the immediate animal source of Middle East Respiratory Syndrome (MERS) epidemic, viruses related to MERS coronavirus (MERS-CoV) have also been found in bats as well as hedgehogs. To elucidate the evolution of MERS-CoV-related viruses and their interspecies transmission pathway, samples were colle...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v11110980
更新日期:2019-10-24 00:00:00
abstract::Hepatitis C is a major global health burden with an estimated 160 million infected individuals worldwide. This long-term disease evolves slowly, often leading to chronicity and potentially to liver failure. There is no anti-HCV vaccine, and, until recently, the only treatment available, based on pegylated interferon a...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v4102197
更新日期:2012-10-17 00:00:00
abstract::Marek's disease virus (MDV) is a member of alphaherpesviruses associated with Marek's disease, a highly contagious neoplastic disease in chickens. Complete sequencing of the viral genome and recombineering techniques using infectious bacterial artificial chromosome (BAC) clones of Marek's disease virus genome have ide...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v10060279
更新日期:2018-05-24 00:00:00
abstract::Viral outbreaks of varying frequencies and severities have caused panic and havoc across the globe throughout history. Influenza, small pox, measles, and yellow fever reverberated for centuries, causing huge burden for economies. The twenty-first century witnessed the most pathogenic and contagious virus outbreaks of ...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v12040465
更新日期:2020-04-20 00:00:00
abstract::For the past two decades, norovirus pandemic variants have emerged every 3⁻5 years, and dominate until they are replaced by alternate strains. However, this scenario changed in 2016 with the co-circulation of six prevalent viruses, three of which possessed the pandemic GII.4 Sydney 2012 capsid. An increased number of ...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v10100548
更新日期:2018-10-09 00:00:00
abstract::Cellular receptor-mediated signaling pathways play critical roles during the initial immune response to Human Cytomegalovirus (HCMV) infection. However, the involvement of type-I transmembrane glycoprotein CD147/EMMPRIN (extracellular matrix metalloproteinase inducer) in the antiviral response to HCMV infection is sti...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v9120365
更新日期:2017-12-01 00:00:00
abstract::The family Totiviridae currently contains five genera Totivirus, Victorivirus, Leishmavirus, Trichomonasvirus, and Giardiavirus. Members in this family generally have a set of two-open reading frame (ORF) elements in their genome with the 5'-proximal ORF (ORF1) encoding a capsid protein (CP) and the 3'-proximal one (O...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v11060577
更新日期:2019-06-25 00:00:00
abstract::A Chikungunya virus (CHIKV) outbreak in Italy in 2007 spread to include the islands of the Caribbean and most of the Americas and still circulates in Europe and Africa. Florida being close in distance to the Caribbean islands experienced a CHIKV outbreak in 2014 and continues to have a few travel-related cases each ye...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12080823
更新日期:2020-07-29 00:00:00
abstract::Angola variant (MARV/Ang) has replaced Mt. Elgon variant Musoke isolate (MARV/MtE-Mus) as the consensus standard variant for Marburg virus research and is regarded as causing a more aggressive phenotype of disease in animal models; however, there is a dearth of published evidence supporting the higher virulence of MAR...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v10110658
更新日期:2018-11-21 00:00:00
abstract::Middle East respiratory syndrome (MERS) coronavirus (MERS-CoV), an infectious coronavirus first reported in 2012, has a mortality rate greater than 35%. Therapeutic antibodies are key tools for preventing and treating MERS-CoV infection, but to date no such agents have been approved for treatment of this virus. Nanobo...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v11020166
更新日期:2019-02-19 00:00:00
abstract::Samples of two Ageratum conyzoides, one Sonchus oleraceus and one turnip (Brassica rapa var. rapa) exhibiting virus-like symptoms were collected from Pakistan and Nepal. Full-length begomovirus clones were obtained from the four plant samples and betasatellite clones from three of these. The begomovirus sequences were...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v7020647
更新日期:2015-02-10 00:00:00
abstract::Most cases of hemorrhagic fever with renal syndrome (HFRS) in Europe are caused by the Puumala hantavirus (PUUV). Typical features of the disease are increased vascular permeability, acute kidney injury (AKI), and thrombocytopenia. YKL-40 is an inflammatory glycoprotein involved in various forms of acute and chronic i...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v11090767
更新日期:2019-08-21 00:00:00
abstract::Persistent circulative transmission of plant viruses involves complex interactions between the transmitted virus and its insect vector. Several studies have shown that insect vector proteins are involved in the passage and the transmission of the virus. Interestingly, proteins expressed by bacterial endosymbionts that...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v5061516
更新日期:2013-06-19 00:00:00
abstract::In Europe, cowpox virus (CPXV) infection in South American camelids occurs as a so-called spill-over infection. Although infected animals generally have a mild form of the disease and survive, cases of fatal generalised CPXV infection have also been described. Prevention by prophylactic vaccination is the only way to ...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12020234
更新日期:2020-02-20 00:00:00
abstract::Lentiviruses are infectious agents of a number of animal species, including sheep, goats, horses, monkeys, cows, and cats, in addition to humans. As in the human case, the host immune response fails to control the establishment of chronic persistent infection that finally leads to a specific disease development. Despi...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v10080435
更新日期:2018-08-17 00:00:00
abstract::We review the molecular and epidemiological characteristics of cetacean morbillivirus (CeMV) and the diagnosis and pathogenesis of associated disease, with six different strains detected in cetaceans worldwide. CeMV has caused epidemics with high mortality in odontocetes in Europe, the USA and Australia. It represents...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v6125145
更新日期:2014-12-22 00:00:00
abstract::West Nile virus (WNV) is a member of the genus Flavivirus in the family Flaviviridae. Flaviviruses replicate in the cytoplasm of infected cells and modify the host cell environment. Although much has been learned about virion structure and virion-endosomal membrane fusion, the cell receptor(s) used have not been defin...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v6010013
更新日期:2013-12-27 00:00:00
abstract::The coronavirus disease 2019 (COVID-19) outbreak caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) affects almost everyone in the world in many ways. We previously predicted antivirals (atazanavir, remdesivir and lopinavir/ritonavir) and non-antiviral drugs (tiotropium and rapamycin) that may inhi...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12070776
更新日期:2020-07-20 00:00:00
abstract::Porcine reproductive and respiratory syndrome virus (PRRSV) infection strongly modulates the host's immune response. The RNA silencing pathway is an intracellular innate response to viral infections. However, it is unknown whether PRRSV interacts with cellular RNA silencing to facilitate the viral infection. Here, we ...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v7102893
更新日期:2015-10-23 00:00:00
abstract::Modified vaccinia virus Ankara (MVA) is the vector of choice for human and veterinary applications due to its strong safety profile and immunogenicity in vivo. The use of MVA and MVA-vectored vaccines against human and animal diseases must comply with regulatory requirements as they pertain to environmental risk asses...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v9110318
更新日期:2017-10-29 00:00:00
abstract::Finding a cure for HIV is challenging because the virus is able to integrate itself into the host cell genome and establish a silent state, called latency, allowing it to evade antiviral drugs and the immune system. Various "shock and kill" strategies are being explored in attempts to eliminate latent HIV reservoirs. ...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v10120677
更新日期:2018-11-30 00:00:00
abstract::Chronic hepatitis C virus (HCV) infection is a major cause of liver disease and hepatocellular carcinoma (HCC)-the second leading, and rising, cause of death due to cancer world-wide [...]. ...
journal_title:Viruses
pub_type: 社论
doi:10.3390/v11050441
更新日期:2019-05-15 00:00:00