Abstract:
:Marek's disease virus (MDV) is a member of alphaherpesviruses associated with Marek's disease, a highly contagious neoplastic disease in chickens. Complete sequencing of the viral genome and recombineering techniques using infectious bacterial artificial chromosome (BAC) clones of Marek's disease virus genome have identified major genes that are associated with pathogenicity. Recent advances in CRISPR/Cas9-based gene editing have given opportunities for precise editing of the viral genome for identifying pathogenic determinants. Here we describe the application of CRISPR/Cas9 gene editing approaches to delete the Meq and pp38 genes from the CVI988 vaccine strain of MDV. This powerful technology will speed up the MDV gene function studies significantly, leading to a better understanding of the molecular mechanisms of MDV pathogenesis.
journal_name
Virusesjournal_title
Virusesauthors
Zhang Y,Tang N,Sadigh Y,Baigent S,Shen Z,Nair V,Yao Ydoi
10.3390/v10060279subject
Has Abstractpub_date
2018-05-24 00:00:00issue
6issn
1999-4915pii
v10060279journal_volume
10pub_type
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