Biochemical and Biophysical Characterization of the dsDNA Packaging Motor from the Lactococcus lactis Bacteriophage Asccphi28.

Abstract:

:Double-stranded DNA viruses package their genomes into pre-assembled protein procapsids. This process is driven by macromolecular motors that transiently assemble at a unique vertex of the procapsid and utilize homomeric ring ATPases to couple genome encapsidation to ATP hydrolysis. Here, we describe the biochemical and biophysical characterization of the packaging ATPase from Lactococcus lactis phage asccφ28. Size-exclusion chromatography (SEC), analytical ultracentrifugation (AUC), small angle X-ray scattering (SAXS), and negative stain transmission electron microscopy (TEM) indicate that the ~45 kDa protein formed a 443 kDa cylindrical assembly with a maximum dimension of ~155 Å and radius of gyration of ~54 Å. Together with the dimensions of the crystallographic asymmetric unit from preliminary X-ray diffraction experiments, these results indicate that gp11 forms a decameric D5-symmetric complex consisting of two pentameric rings related by 2-fold symmetry. Additional kinetic analysis shows that recombinantly expressed gp11 has ATPase activity comparable to that of functional ATPase rings assembled on procapsids in other genome packaging systems. Hence, gp11 forms rings in solution that likely reflect the fully assembled ATPases in active virus-bound motor complexes. Whereas ATPase functionality in other double-stranded DNA (dsDNA) phage packaging systems requires assembly on viral capsids, the ability to form functional rings in solution imparts gp11 with significant advantages for high-resolution structural studies and rigorous biophysical/biochemical analysis.

journal_name

Viruses

journal_title

Viruses

authors

Reyes-Aldrete E,Dill EA,Bussetta C,Szymanski MR,Diemer G,Maindola P,White MA,Bujalowski WM,Choi KH,Morais MC

doi

10.3390/v13010015

subject

Has Abstract

pub_date

2020-12-23 00:00:00

issue

1

issn

1999-4915

pii

v13010015

journal_volume

13

pub_type

杂志文章
  • Bioinformatics Pipeline for Human Papillomavirus Short Read Genomic Sequences Classification Using Support Vector Machine.

    abstract::We recently developed a test based on the Agilent SureSelect target enrichment system capturing genomic fragments from 191 human papillomaviruses (HPV) types for Illumina sequencing. This enriched whole genome sequencing (eWGS) assay provides an approach to identify all HPV types in a sample. Here we present a machine...

    journal_title:Viruses

    pub_type: 杂志文章

    doi:10.3390/v12070710

    authors: Lomsadze A,Li T,Rajeevan MS,Unger ER,Borodovsky M

    更新日期:2020-06-30 00:00:00

  • Characterization of the RNA Transcription Profile of Bombyx mori Bidensovirus.

    abstract::Bombyx mori bidensovirus (BmBDV) is a single-stranded DNA (ssDNA) virus from the genus Bidensovirus of the Bidnaviridae family, which, thus far, solely infects insects. It has a unique genome that contains bipartite DNA molecules (VD1 and VD2). In this study, we explored the detailed transcription mapping of the compl...

    journal_title:Viruses

    pub_type: 杂志文章

    doi:10.3390/v11040325

    authors: Li R,Chang P,Lü P,Hu Z,Chen K,Yao Q,Yu Q

    更新日期:2019-04-03 00:00:00

  • Pteropine Orthoreovirus in an Angolan Soft-Furred Fruit Bat (Lissonycteris angolensis) in Uganda Dramatically Expands the Global Distribution of an Emerging Bat-Borne Respiratory Virus.

    abstract::Pteropine orthoreovirus (PRV; Reoviridae: Spinareovirinae) is an emerging bat-borne zoonotic virus that causes influenza-like illness (ILI). PRV has thus far been found only in Australia and Asia, where diverse old-world fruit bats (Pteropodidae) serve as hosts. In this study, we report the discovery of PRV in Africa,...

    journal_title:Viruses

    pub_type: 杂志文章

    doi:10.3390/v12070740

    authors: Bennett AJ,Goldberg TL

    更新日期:2020-07-09 00:00:00

  • A3R Phage and Staphylococcus aureus Lysate Do Not Induce Neutrophil Degranulation.

    abstract::The objective of this study was to evaluate the effects of A3R phage and Staphylococcus aureus lysate obtained after phage infection on neutrophil degranulation. The exocytosis of primary and secondary granules from neutrophils was investigated in vitro in whole blood specimens by flow cytometry based on the expressio...

    journal_title:Viruses

    pub_type: 杂志文章

    doi:10.3390/v9020036

    authors: Borysowski J,Międzybrodzki R,Wierzbicki P,Kłosowska D,Korczak-Kowalska G,Weber-Dąbrowska B,Górski A

    更新日期:2017-02-21 00:00:00

  • The Impact of HIV-1 Genetic Diversity on CRISPR-Cas9 Antiviral Activity and Viral Escape.

    abstract::The clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 system is widely explored for sequence-specific attack on HIV-1 proviral DNA. We recently identified dual-guide RNA (dual-gRNA) combinations that can block HIV-1 replication permanently in infected cell cultures and prevent viral escape. Altho...

    journal_title:Viruses

    pub_type: 杂志文章

    doi:10.3390/v11030255

    authors: Darcis G,Binda CS,Klaver B,Herrera-Carrillo E,Berkhout B,Das AT

    更新日期:2019-03-13 00:00:00

  • Unprecedented Diversity of Lactococcal Group 936 Bacteriophages Revealed by Amplicon Sequencing of the Portal Protein Gene.

    abstract::Lactococcus lactis is one of the most important bacteria in dairy fermentations, being used in the production of cheese and buttermilk. The processes are vulnerable to phage attacks, and undefined mixtures of lactococcal strains are often used to reduce the risk of bacteriophage caused fermentation failure. Other prev...

    journal_title:Viruses

    pub_type: 杂志文章

    doi:10.3390/v11050443

    authors: Frantzen CA,Holo H

    更新日期:2019-05-16 00:00:00

  • Experimental Usutu Virus Infection in Domestic Canaries Serinus canaria.

    abstract::Usutu virus (USUV) is a neurotropic flavivirus closely related to West Nile virus (WNV). Its enzootic cycle mainly involves mosquitoes and birds. Human infection can occur with occasional, but sometimes severe, neurological complications. Since its emergence and spread in Europe over the last two decades, USUV has bee...

    journal_title:Viruses

    pub_type: 杂志文章

    doi:10.3390/v12020164

    authors: Benzarti E,Rivas J,Sarlet M,Franssen M,Desmecht D,Schmidt-Chanasit J,Savini G,Lorusso A,Van Laere AS,Garigliany MM

    更新日期:2020-01-31 00:00:00

  • A Taxonomic Review of Clostridium difficile Phages and Proposal of a Novel Genus, "Phimmp04likevirus".

    abstract::Currently, only three phages that infect the medically important bacterium Clostridium difficile have been discussed by the International Committee of Viral Taxonomy (ICTV). They are all myoviruses, and have been assigned to the genus "phicd119likevirus". An additional nine phages have since been described in the lite...

    journal_title:Viruses

    pub_type: 杂志文章

    doi:10.3390/v7052534

    authors: Hargreaves KR,Clokie MR

    更新日期:2015-05-21 00:00:00

  • Phylogenomic Analysis of Global Isolates of Canid Alphaherpesvirus 1.

    abstract::Canid alphaherpesvirus 1 (CHV-1) is a widespread pathogen of dogs with multiple associated clinical signs. There has been limited prior investigation into the genomics and phylogeny of this virus using whole viral genome analysis. Fifteen CHV-1 isolates were collected from animals with ocular disease based in the USA....

    journal_title:Viruses

    pub_type: 杂志文章

    doi:10.3390/v12121421

    authors: Lewin AC,Coghill LM,Mironovich M,Liu CC,Carter RT,Ledbetter EC

    更新日期:2020-12-10 00:00:00

  • Shedding Light on the Role of Extracellular Vesicles in HIV Infection and Wound Healing.

    abstract::Extracellular vesicles (EVs) play an important role in intercellular communication. They are naturally released from cells into the extracellular environment. Based on their biogenesis, release pathways, size, content, and function, EVs are classified into exosomes, microvesicles (MVs), and apoptotic bodies (ApoBDs). ...

    journal_title:Viruses

    pub_type: 杂志文章,评审

    doi:10.3390/v12060584

    authors: Alqatawni A,Sharma AL,Attilus B,Tyagi M,Daniel R

    更新日期:2020-05-27 00:00:00

  • The role of RNA interference (RNAi) in arbovirus-vector interactions.

    abstract::RNA interference (RNAi) was shown over 18 years ago to be a mechanism by which arbovirus replication and transmission could be controlled in arthropod vectors. During the intervening period, research on RNAi has defined many of the components and mechanisms of this antiviral pathway in arthropods, yet a number of unex...

    journal_title:Viruses

    pub_type: 杂志文章,评审

    doi:10.3390/v7020820

    authors: Blair CD,Olson KE

    更新日期:2015-02-17 00:00:00

  • Estimating hantavirus risk in southern Argentina: a GIS-based approach combining human cases and host distribution.

    abstract::We use a Species Distribution Modeling (SDM) approach along with Geographic Information Systems (GIS) techniques to examine the potential distribution of hantavirus pulmonary syndrome (HPS) caused by Andes virus (ANDV) in southern Argentina and, more precisely, define and estimate the area with the highest infection p...

    journal_title:Viruses

    pub_type: 杂志文章

    doi:10.3390/v6010201

    authors: Andreo V,Neteler M,Rocchini D,Provensal C,Levis S,Porcasi X,Rizzoli A,Lanfri M,Scavuzzo M,Pini N,Enria D,Polop J

    更新日期:2014-01-14 00:00:00

  • Dysregulated Interferon Response Underlying Severe COVID-19.

    abstract::Innate immune interferons (IFNs), including type I and III IFNs, constitute critical antiviral mechanisms. Recent studies reveal that IFN dysregulation is key to determine COVID-19 pathogenesis. Effective IFN stimulation or prophylactic administration of IFNs at the early stage prior to severe COVID-19 may elicit an a...

    journal_title:Viruses

    pub_type: 杂志文章,评审

    doi:10.3390/v12121433

    authors: Lopez L,Sang PC,Tian Y,Sang Y

    更新日期:2020-12-13 00:00:00

  • In Vitro Synthesized RNA Generated from cDNA Clones of Both Genomic Components of Cucurbit yellow stunting disorder virus Replicates in Cucumber Protoplasts.

    abstract::Cucurbit yellow stunting disorder virus (CYSDV), a bipartite whitefly-transmitted virus, constitutes a major threat to commercial cucurbit production worldwide. Here, construction of full-length CYSDV RNA1 and RNA2 cDNA clones allowed the in vitro synthesis of RNA transcripts able to replicate in cucumber protoplasts....

    journal_title:Viruses

    pub_type: 杂志文章

    doi:10.3390/v8060170

    authors: Owen CA,Moukarzel R,Huang X,Kassem MA,Eliasco E,Aranda MA,Coutts RH,Livieratos IC

    更新日期:2016-06-14 00:00:00

  • The Roles of Ebola Virus Soluble Glycoprotein in Replication, Pathogenesis, and Countermeasure Development.

    abstract::Ebola virus (EBOV) is a highly lethal pathogen that has caused several outbreaks of severe hemorrhagic fever in humans since its emergence in 1976. The EBOV glycoprotein (GP1,2) is the sole viral envelope protein and a major component of immunogenicity; it is encoded by the GP gene along with two truncated versions: s...

    journal_title:Viruses

    pub_type: 杂志文章,评审

    doi:10.3390/v11110999

    authors: Zhu W,Banadyga L,Emeterio K,Wong G,Qiu X

    更新日期:2019-10-31 00:00:00

  • Dendritic Cells in HIV/SIV Prophylactic and Therapeutic Vaccination.

    abstract::Dendritic cells (DCs) are involved in human and simian immunodeficiency virus (HIV and SIV) pathogenesis but also play a critical role in orchestrating innate and adaptive vaccine-specific immune responses. Effective HIV/SIV vaccines require strong antigen-specific CD4 T cell responses, cytotoxic activity of CD8 T cel...

    journal_title:Viruses

    pub_type: 杂志文章,评审

    doi:10.3390/v12010024

    authors: Ko EJ,Robert-Guroff M

    更新日期:2019-12-24 00:00:00

  • Field Trial Vaccination against Cowpox in Two Alpaca Herds.

    abstract::In Europe, cowpox virus (CPXV) infection in South American camelids occurs as a so-called spill-over infection. Although infected animals generally have a mild form of the disease and survive, cases of fatal generalised CPXV infection have also been described. Prevention by prophylactic vaccination is the only way to ...

    journal_title:Viruses

    pub_type: 杂志文章

    doi:10.3390/v12020234

    authors: Prkno A,Hoffmann D,Kaiser M,Goerigk D,Pfeffer M,Winter K,Vahlenkamp TW,Beer M,Starke A

    更新日期:2020-02-20 00:00:00

  • Hepatitis C virus and natural compounds: a new antiviral approach?

    abstract::Hepatitis C is a major global health burden with an estimated 160 million infected individuals worldwide. This long-term disease evolves slowly, often leading to chronicity and potentially to liver failure. There is no anti-HCV vaccine, and, until recently, the only treatment available, based on pegylated interferon a...

    journal_title:Viruses

    pub_type: 杂志文章,评审

    doi:10.3390/v4102197

    authors: Calland N,Dubuisson J,Rouillé Y,Séron K

    更新日期:2012-10-17 00:00:00

  • Overview of the Nucleic-Acid Binding Properties of the HIV-1 Nucleocapsid Protein in Its Different Maturation States.

    abstract::HIV-1 Gag polyprotein orchestrates the assembly of viral particles. Its C-terminus consists of the nucleocapsid (NC) domain that interacts with nucleic acids, and p1 and p6, two unstructured regions, p6 containing the motifs to bind ALIX, the cellular ESCRT factor TSG101 and the viral protein Vpr. The processing of Ga...

    journal_title:Viruses

    pub_type: 杂志文章,评审

    doi:10.3390/v12101109

    authors: Mouhand A,Pasi M,Catala M,Zargarian L,Belfetmi A,Barraud P,Mauffret O,Tisné C

    更新日期:2020-09-29 00:00:00

  • Overexpression of MAP2 and NF-H Associated with Dendritic Pathology in the Spinal Cord of Mice Infected with Rabies Virus.

    abstract::Rabies is a viral infection that targets the nervous system, specifically neurons. The clinical manifestations of the disease are dramatic and their outcome fatal; paradoxically, conventional histopathological descriptions reveal only subtle changes in the affected nervous tissue. Some researchers have considered that...

    journal_title:Viruses

    pub_type: 杂志文章

    doi:10.3390/v10030112

    authors: Monroy-Gómez J,Santamaría G,Torres-Fernández O

    更新日期:2018-03-06 00:00:00

  • Addressing the Challenges of Hepatitis C Cure and Persistent Risk of Hepatocellular Carcinoma.

    abstract::Chronic hepatitis C virus (HCV) infection is a major cause of liver disease and hepatocellular carcinoma (HCC)-the second leading, and rising, cause of death due to cancer world-wide [...]. ...

    journal_title:Viruses

    pub_type: 社论

    doi:10.3390/v11050441

    authors: Baumert TF,Hoshida Y

    更新日期:2019-05-15 00:00:00

  • Exploratory Spatial Analysis of in vitro Respiratory Syncytial Virus Co-infections.

    abstract::The cell response to virus infection and virus perturbation of that response is dynamic and is reflected by changes in cell susceptibility to infection. In this study, we evaluated the response of human epithelial cells to sequential infections with human respiratory syncytial virus strains A2 and B to determine if a ...

    journal_title:Viruses

    pub_type: 杂志文章

    doi:10.3390/v2122782

    authors: Simeonov I,Gong X,Kim O,Poss M,Chiaromonte F,Fricks J

    更新日期:2010-12-01 00:00:00

  • Nucleocapsid Structure of Negative Strand RNA Virus.

    abstract::Negative strand RNA viruses (NSVs) include many important human pathogens, such as influenza virus, Ebola virus, and rabies virus. One of the unique characteristics that NSVs share is the assembly of the nucleocapsid and its role in viral RNA synthesis. In NSVs, the single strand RNA genome is encapsidated in the line...

    journal_title:Viruses

    pub_type: 杂志文章,评审

    doi:10.3390/v12080835

    authors: Luo M,Terrell JR,Mcmanus SA

    更新日期:2020-07-30 00:00:00

  • The Role of Dendritic Cells in Immune Control and Vaccination against -Herpesviruses.

    abstract::The two human oncogenic -herpesviruses, Epstein Barr virus (EBV) and Kaposi sarcoma-associated herpesvirus (KSHV), are prototypic pathogens that are controlled by T cell responses. Despite their ubiquitous distribution, persistent infections and transforming potential, most carriers' immune systems control them for li...

    journal_title:Viruses

    pub_type: 杂志文章,评审

    doi:10.3390/v11121125

    authors: Münz C

    更新日期:2019-12-05 00:00:00

  • Epstein-Barr Virus-Induced Post-Transplant Lymphoproliferative Disorder of the Central Nervous System Successfully Treated with Chemo-immunotherapy.

    abstract::Aplastic anemia is a rare blood disease characterized by the destruction of the hematopoietic stem cells (HSC) in the bone marrow that, in the majority of cases, is caused by an autoimmune reaction. Patients with aplastic anemia are treated with immunosuppressive drugs and some of them, especially younger individuals ...

    journal_title:Viruses

    pub_type: 杂志文章

    doi:10.3390/v12040416

    authors: Inoue H,Rai S,Tanaka H,Espinoza JL,Komori-Inoue M,Kakutani H,Minamoto S,Kumode T,Nakayama S,Taniguchi Y,Morita Y,Okuda T,Tatsumi Y,Ashida T,Matsumura I

    更新日期:2020-04-08 00:00:00

  • Influenza Virus Infections and Cellular Kinases.

    abstract::Influenza A viruses (IAVs) are a major cause of respiratory illness and are responsible for yearly epidemics associated with more than 500,000 annual deaths globally. Novel IAVs may cause pandemic outbreaks and zoonotic infections with, for example, highly pathogenic avian influenza virus (HPAIV) of the H5N1 and H7N9 ...

    journal_title:Viruses

    pub_type: 杂志文章,评审

    doi:10.3390/v11020171

    authors: Meineke R,Rimmelzwaan GF,Elbahesh H

    更新日期:2019-02-20 00:00:00

  • "FastCheckFLI PPR-like"-A Molecular Tool for the Fast Genome Detection of PPRV and Differential Diagnostic Pathogens.

    abstract::To assist the global eradication of peste des petits ruminants virus (PPRV), a molecular test for the rapid and reliable detection of PPRV was developed which additionally enables the detection of pathogens relevant for differential diagnostics. For this purpose, the necessary time frame of a magnetic bead-based nucle...

    journal_title:Viruses

    pub_type: 杂志文章

    doi:10.3390/v12111227

    authors: Halecker S,Mettenleiter TC,Beer M,Hoffmann B

    更新日期:2020-10-29 00:00:00

  • Increased HIV Subtype Diversity Reflecting Demographic Changes in the HIV Epidemic in New South Wales, Australia.

    abstract::Changes over time in HIV-1 subtype diversity within a population reflect changes in factors influencing the development of local epidemics. Here we report on the genetic diversity of 2364 reverse transcriptase sequences from people living with HIV-1 in New South Wales (NSW) notified between 2004 and 2018. These data r...

    journal_title:Viruses

    pub_type: 杂志文章

    doi:10.3390/v12121402

    authors: Di Giallonardo F,Pinto AN,Keen P,Shaik A,Carrera A,Salem H,Selvey C,Nigro SJ,Fraser N,Price K,Holden J,Lee FJ,Dwyer DE,Bavinton BR,Grulich AE,Kelleher AD,On Behalf Of The Nsw Hiv Prevention Partnership Project

    更新日期:2020-12-06 00:00:00

  • Characterization of a Novel Megalocytivirus Isolated from European Chub (Squalius cephalus).

    abstract::A novel virus from moribund European chub (Squalius cephalus) was isolated on epithelioma papulosum cyprini (EPC) cells. Transmission electron microscopic examination revealed abundant non-enveloped, hexagonal virus particles in the cytoplasm of infected EPC cells consistent with an iridovirus. Illumina MiSeq sequence...

    journal_title:Viruses

    pub_type: 杂志文章

    doi:10.3390/v11050440

    authors: Halaly MA,Subramaniam K,Koda SA,Popov VL,Stone D,Way K,Waltzek TB

    更新日期:2019-05-15 00:00:00

  • An Emerging Human Parechovirus Type 5 Causing Sepsis-Like Illness in Infants in Australia.

    abstract::Human parechovirus (HPeV), particularly type 3 (HPeV3), is an important cause of sepsis-/meningitis-like illness in young infants. Laboratory records identified a total of ten HPeV-positive cases in Southeastern Australia between January and July 2019. The HPeV present in these cases were typed by Sanger sequencing of...

    journal_title:Viruses

    pub_type: 杂志文章

    doi:10.3390/v11100913

    authors: Chamings A,Liew KC,Reid E,Athan E,Raditsis A,Vuillermin P,Yoga Y,Caly L,Druce J,Alexandersen S

    更新日期:2019-10-03 00:00:00