Abstract：
:Internal ribosome entry is a key mechanism for viral protein synthesis in a subset of RNA viruses. Cricket paralysis virus (CrPV), a member of Dicistroviridae, has a positive-sense single strand RNA genome that contains two internal ribosome entry sites (IRES), a 5'untranslated region (5'UTR) and intergenic region (IGR) IRES, that direct translation of open reading frames (ORF) encoding the viral non-structural and structural proteins, respectively. The regulation of and the significance of the CrPV IRESs during infection are not fully understood. In this study, using a series of biochemical assays including radioactive-pulse labelling, reporter RNA assays and ribosome profiling, we demonstrate that while 5'UTR IRES translational activity is constant throughout infection, IGR IRES translation is delayed and then stimulated two to three hours post infection. The delay in IGR IRES translation is not affected by inhibiting global translation prematurely via treatment with Pateamine A. Using a CrPV replicon that uncouples viral translation and replication, we show that the increase in IGR IRES translation is dependent on expression of non-structural proteins and is greatly stimulated when replication is active. Temporal regulation by distinct IRESs within the CrPV genome is an effective viral strategy to ensure optimal timing and expression of viral proteins to facilitate infection.
journal_name
Virusesjournal_title
Virusesauthors
Khong A,Bonderoff JM,Spriggs RV,Tammpere E,Kerr CH,Jackson TJ,Willis AE,Jan Edoi
10.3390/v8010025subject
Has Abstractpub_date
2016-01-19 00:00:00issue
1issn
1999-4915pii
v8010025journal_volume
8pub_type
杂志文章相关文献
Viruses-Basel文献大全abstract：:To further understanding of the structure and morphology of the Orthohantavirus, family Hantaviridae, we have employed cryo-electron microscopy (cryo-EM) for three New World hantaviruses: Andes (ANDV), Sin Nombre (SNV), and Black Creek Canal (BCCV). Building upon our prior cryo-EM and cryo-tomography study of the Old ...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v11090862
更新日期：2019-09-16 00:00:00
abstract：:Respiratory viral infections are strongly associated with asthma exacerbations. Rhinovirus is most frequently-detected pathogen; followed by respiratory syncytial virus; metapneumovirus; parainfluenza virus; enterovirus and coronavirus. In addition; viral infection; in combination with genetics; allergen exposure; mic...
journal_title：Viruses
pub_type： 杂志文章,评审
doi：10.3390/v10120682
更新日期：2018-12-01 00:00:00
abstract：:Global climate change-induced warming of the Artic seas is predicted to shift the phytoplankton community towards dominance of smaller-sized species due to global warming. Yet, little is known about their viral mortality agents despite the ecological importance of viruses regulating phytoplankton host dynamics and div...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v9060134
更新日期：2017-06-02 00:00:00
abstract：:As countries with endemic canine rabies progress towards elimination by 2030, it will become necessary to employ techniques to help plan, monitor, and confirm canine rabies elimination. Sequencing can provide critical information to inform control and vaccination strategies by identifying genetically distinct virus va...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v12111255
更新日期：2020-11-04 00:00:00
abstract：:Lettuce is often implicated in human norovirus (HuNoV) foodborne outbreaks. We identified H-like histo-blood group antigens (HBGAs) on lettuce leaves as specific binding moieties for virus-like particles (VLPs) of HuNoV GII.4/HS194/2009 strain. The objective of this study was to determine whether HuNoV-lettuce binding...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v11090833
更新日期：2019-09-08 00:00:00
abstract：:A novel virus from moribund European chub (Squalius cephalus) was isolated on epithelioma papulosum cyprini (EPC) cells. Transmission electron microscopic examination revealed abundant non-enveloped, hexagonal virus particles in the cytoplasm of infected EPC cells consistent with an iridovirus. Illumina MiSeq sequence...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v11050440
更新日期：2019-05-15 00:00:00
abstract：:Human immunodeficiency virus (HIV) is a causative agent of acquired immune deficiency syndrome (AIDS). Highly active antiretroviral therapy (HAART) can slow down the replication of HIV-1, leading to an improvement in the survival of HIV-1-infected patients. However, drug toxicities and poor drug administration has led...
journal_title：Viruses
pub_type： 杂志文章,评审
doi：10.3390/v9100281
更新日期：2017-09-29 00:00:00
abstract：:Begomoviruses are one of the major groups of plant viruses with an important economic impact on crop production in tropical and subtropical regions. The global spread of its polyphagous vector, the whitefly Bemisia tabaci, has contributed to the emergence and diversification of species within this genus. In this study...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v11040327
更新日期：2019-04-04 00:00:00
abstract：:Dengue virus (DENV) is the most widespread arthropod-borne virus, with the number and severity of outbreaks increasing worldwide in recent decades. Dengue is caused by genetically distinct serotypes, DENV-1-4. Here, we present data on DENV-1, isolated from patients with dengue fever during an outbreak in Senegal and M...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v13010057
更新日期：2021-01-04 00:00:00
abstract：:Middle East respiratory syndrome (MERS) coronavirus (MERS-CoV), an infectious coronavirus first reported in 2012, has a mortality rate greater than 35%. Therapeutic antibodies are key tools for preventing and treating MERS-CoV infection, but to date no such agents have been approved for treatment of this virus. Nanobo...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v11020166
更新日期：2019-02-19 00:00:00
abstract：:During flavivirus infection, some viral proteins move to the nucleus and cellular components are relocated from the nucleus to the cytoplasm. Thus, the integrity of the main regulator of the nuclear-cytoplasmic transport, the nuclear pore complex (NPC), was evaluated during infection with dengue virus (DENV) and Zika ...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v12060583
更新日期：2020-05-26 00:00:00
abstract：:One hundred Prunus trees, including almond (P. dulcis), apricot (P. armeniaca), nectarine (P. persica var. nucipersica), peach (P. persica), plum (P. domestica), purple leaf plum (P. cerasifera) and sweet cherry (P. avium), were selected from growing regions Australia-wide and tested for the presence of 34 viruses and...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v12020246
更新日期：2020-02-23 00:00:00
abstract：:An outbreak of winter dysentery, complicated by severe respiratory syndrome, occurred in January 2020 in a high production dairy cow herd located in a hilly area of the Calabria region. Of the 52 animals belonging to the farm, 5 (9.6%) died with severe respiratory distress, death occurring 3-4 days after the appearanc...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v12111331
更新日期：2020-11-19 00:00:00
abstract：:Since deer were introduced into Australia in the mid-1800s, their wild populations have increased in size and distribution, posing a potential risk to the livestock industry, through their role in pathogen transmission cycles. In comparison to livestock, there are limited data on viral infections in all wildlife, incl...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v12070752
更新日期：2020-07-13 00:00:00
abstract：:It is difficult to observe the molecular choreography between viruses and host cell components, as they exist on a spatial scale beyond the reach of conventional microscopy. However, novel super-resolution microscopy techniques have cast aside technical limitations to reveal a nanoscale view of virus replication and c...
journal_title：Viruses
pub_type： 杂志文章,评审
doi：10.3390/v6031365
更新日期：2014-03-19 00:00:00
abstract：:Hantaviruses are an established cause of haemorrhagic fever with renal syndrome (HFRS) in Europe. Following a confirmed case of HFRS in the UK, in an individual residing on a farm in North Yorkshire and the Humber, a tidal estuary on the east coast of Northern England, and the subsequent isolation of a Seoul hantaviru...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v6020524
更新日期：2014-02-05 00:00:00
abstract：:Noroviruses are the most common etiological agent of acute gastroenteritis worldwide. Despite their high infectivity, a subpopulation of individuals is resistant to infection and disease. This susceptibility is norovirus genotype-dependent and is largely mediated by the presence or absence of human histo-blood group a...
journal_title：Viruses
pub_type： 杂志文章,评审
doi：10.3390/v11030226
更新日期：2019-03-06 00:00:00
abstract：:Iminosugars that are competitive inhibitors of endoplasmic reticulum (ER) α-glucosidases have been demonstrated to have antiviral activity against a diverse set of viruses. A novel iminosugar, UV-4B, has recently been shown to provide protection against lethal infections with dengue and influenza A (H1N1) viruses in m...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v8030071
更新日期：2016-03-07 00:00:00
abstract：:Zika virus (ZIKV) infection during pregnancy leads to severe congenital Zika syndrome, which includes microcephaly and other neurological malformations. No therapeutic agents have, so far, been approved for the treatment of ZIKV infection in humans; as such, there is a need for a continuous effort to develop effective...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v11111019
更新日期：2019-11-02 00:00:00
abstract：:RNA secondary structures play diverse roles in positive-sense (+) RNA virus infections, but those located with the replication protein coding sequence can be difficult to investigate. Structures that regulate the translation of replication proteins pose particular challenges, as their potential involvement in post-tra...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v12030350
更新日期：2020-03-22 00:00:00
abstract：:We show that focused ion beam/scanning electron microscopy (FIB/SEM) tomography is an excellent method to analyze the three-dimensional structure of a fibroblast nucleus infected with human cytomegalovirus (HCMV). We found that the previously described infoldings of the inner nuclear membrane, which are unique among i...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v7112900
更新日期：2015-11-04 00:00:00
abstract：:Puumala virus (PUUV) is the agent of nephropathia epidemica (NE), a mild form of hemorrhagic fever with renal syndrome (HFRS) in Europe. NE incidence presents a high spatial variation throughout France, while the geographical distribution of the wild reservoir of PUUV, the bank vole, is rather continuous. A missing pi...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v7102884
更新日期：2015-10-22 00:00:00
abstract：:Most cases of hemorrhagic fever with renal syndrome (HFRS) in Europe are caused by the Puumala hantavirus (PUUV). Typical features of the disease are increased vascular permeability, acute kidney injury (AKI), and thrombocytopenia. YKL-40 is an inflammatory glycoprotein involved in various forms of acute and chronic i...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v11090767
更新日期：2019-08-21 00:00:00
abstract：:Virus transmission is essential for spreading viral infections and is a highly coordinated process which occurs by cell-free transmission or cell-cell contact. The transmission of Bovine Foamy Virus (BFV) is highly cell-associated, with undetectable cell-free transmission. However, BFV particle budding can be induced ...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v7112907
更新日期：2015-11-11 00:00:00
abstract：:Sequence-independent amplification techniques have become important tools for virus discovery, metagenomics, and exploration of viral diversity at the global scale, especially in remote areas. Here, we describe the detection and genetic characterization of a novel gyrovirus, named GyV11, present in cloacal, oral, and ...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v11121148
更新日期：2019-12-11 00:00:00
abstract：:Background: Bats are known to host a number of nonpathogenic viruses, as well as highly pathogenic viruses causing fatal diseases like rabies. Serological surveys as part of active and passive bat rabies surveillance mainly use seroneutralization assays, demonstrating the presence of lyssavirus-specific antibodies in ...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v12030271
更新日期：2020-02-28 00:00:00
abstract：:Multi-drug resistance is increasing at alarming rates. The efficacy of phage therapy, treating bacterial infections with bacteriophages alone or in combination with traditional antibiotics, has been demonstrated in emergency cases in the United States and in other countries, however remains to be approved for wide-spr...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v10040188
更新日期：2018-04-10 00:00:00
abstract：:Hepatitis B virus (HBV) persistence is at the basis of HBV reactivation as a consequence of chemotherapy and immunosuppressive treatments. The identification of early viral replication indicators and markers of effective HBV immunological control would be useful in monitoring patients who are at risk of potential vira...
journal_title：Viruses
pub_type： 杂志文章,评审
doi：10.3390/v11090783
更新日期：2019-08-25 00:00:00
abstract：:Phylodynamic inference is a pivotal tool in understanding transmission dynamics of viral outbreaks. These analyses are strongly guided by the input of an epidemiological model as well as sequence data that must contain sufficient intersequence variability in order to be informative. These criteria, however, may not be...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v13010079
更新日期：2021-01-08 00:00:00
abstract：:Progression of HIV infection is variable among individuals, and definition disease progression biomarkers is still needed. Here, we aimed to categorize the predictive potential of several variables using feature selection methods and decision trees. A total of seventy-five treatment-naïve subjects were enrolled during...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v10010034
更新日期：2018-01-13 00:00:00
