Abstract:
:Salmonid alphavirus (SAV) is the cause of pancreas disease and sleeping disease in farmed salmonid fish in Europe. The spread of these diseases has been difficult to control with biosecurity and current vaccination strategies, and increased understanding of the viral pathogenesis could be beneficial for the development of novel vaccine strategies. N-glycosylation of viral envelope proteins may be crucial for viral virulence and a possible target for its purposed attenuation. In this study, we mutated the N-glycosylation consensus motifs of the E1 and E2 glycoproteins of a SAV3 infectious clone using site-directed mutagenesis. Mutation of the glycosylation motif in E1 gave a complete inactivation of the virus as no viral replication could be detected in cell culture and infectious particles could not be rescued. In contrast, infectious virus particles could be recovered from the SAV3 E2 mutants (E2319Q, E2319A), but not if they were accompanied by lack of N-glycosylation in E1. Compared to the non-mutated infectious clone, the SAV3-E2319Q and SAV3-E2319A recombinant viruses produced less cytopathic effects in cell culture and lower amounts of infectious viral particles. In conclusion, the substitution in the N-linked glycosylation site in E2 attenuated SAV3 in cell culture. The findings could be useful for immunization strategies using live attenuated vaccines and testing in fish will be desirable to study the clone's properties in vivo.
journal_name
Virusesjournal_title
Virusesauthors
Aksnes I,Markussen T,Braaen S,Rimstad Edoi
10.3390/v12101071subject
Has Abstractpub_date
2020-09-24 00:00:00issue
10issn
1999-4915pii
v12101071journal_volume
12pub_type
杂志文章相关文献
Viruses-Basel文献大全abstract::Human coronaviruses cause both upper and lower respiratory tract infections in humans. In 2012, a sixth human coronavirus (hCoV) was isolated from a patient presenting with severe respiratory illness. The 60-year-old man died as a result of renal and respiratory failure after admission to a hospital in Jeddah, Saudi A...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v10020093
更新日期:2018-02-24 00:00:00
abstract::Equine influenza, caused by the H3N8 subtype, is a highly contagious respiratory disease affecting equid populations worldwide and has led to serious epidemics and transboundary pandemics. This study describes the phylogenetic characterization and replication kinetics of recently-isolated H3N8 virus from a nasal swab ...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v10010031
更新日期:2018-01-11 00:00:00
abstract::The orthopoxviruses (OPV) comprise several emerging viruses with great importance to human and veterinary medicine, including vaccinia virus (VACV), which causes outbreaks of bovine vaccinia (BV) in South America. Historically, VACV is the most comprehensively studied virus, however, its origin and natural hosts remai...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v9110340
更新日期:2017-11-15 00:00:00
abstract::Zika virus (ZIKV) is a vertically and sexually transmissible virus resulting in severe congenital malformation. The goal of this study was to develop an ovine model of ZIKV infection. Between 28-35 days gestation (DG), four pregnant animals were infected with two doses of 6 × 106 PFU of ZIKV; four control animals rece...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v11090795
更新日期:2019-08-29 00:00:00
abstract::Retroviruses integrate a reverse transcribed double stranded DNA copy of their viral genome into the chromosomal DNA of cells they infect. Occasionally, exogenous retroviruses infect germ cells and when this happens a profound shift in the virus host dynamic occurs. Retroviruses maintained as hereditable viral genetic...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v3060901
更新日期:2011-06-01 00:00:00
abstract::Infectious bursal disease (IBD) is an immunosuppressive, highly contagious, and lethal disease of young chickens caused by IBD virus (IBDV). It results in huge economic loss to the poultry industry worldwide. Infection caused by very virulent IBDV (vvIBDV) strains results in high mortality in young chicken flocks. How...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12020249
更新日期:2020-02-23 00:00:00
abstract::Virus-induced inflammation plays a critical role in determining the clinical outcome of an acute respiratory virus infection. We have shown previously that the administration of immunobiotic Lactobacillus plantarum (Lp) directly to the respiratory tract prevents lethal inflammatory responses to subsequent infection wi...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v13010002
更新日期:2020-12-22 00:00:00
abstract::Dengue virus (DENV) infection has become a critically important globally prevalent infectious disease, especially in tropical and subtropical countries. Since neither currently exists, there is an urgent need for an effective vaccine to prevent, and a specific drug to treat DENV infection. Therapeutic peptides represe...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12111267
更新日期:2020-11-06 00:00:00
abstract::The extension of virology beyond its traditional medical, veterinary, or agricultural applications, now called environmental virology, has shown that viruses are both the most numerous and diverse biological entities on Earth. In particular, virus isolations from unicellular eukaryotic hosts (heterotrophic and photosy...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12101130
更新日期:2020-10-06 00:00:00
abstract::Tetherin is an interferon-induced, intrinsic cellular response factor that blocks release of numerous viruses, including Ebola virus, from infected cells. As with many viruses targeted by host factors, Ebola virus employs a tetherin antagonist, the viral glycoprotein (EboGP), to counteract restriction and promote viru...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v7102888
更新日期:2015-10-26 00:00:00
abstract::We mapped the hemagglutinin-esterase (HE) antigenic epitopes of the influenza C virus on the three-dimensional (3D) structure of the HE glycoprotein using 246 escape mutants that were selected by a panel of nine anti-HE monoclonal antibodies (MAbs), including seven of the C/Ann Arbor/1/50 virus and two of the C/Yamaga...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v10080417
更新日期:2018-08-09 00:00:00
abstract::Flaviviruses are enveloped arthropod-borne viruses with a single-stranded, positive-sense RNA genome that can cause serious illness in humans and animals. The 11 kb 5' capped RNA genome consists of a single open reading frame (ORF), and is flanked by 5' and 3' untranslated regions (UTR). The ORF is a polyprotein that ...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v9060137
更新日期:2017-06-06 00:00:00
abstract::Chikungunya virus (CHIKV) has caused extensive outbreaks in several countries within the Americas, Asia, Oceanic/Pacific Islands, and Europe. In humans, CHIKV infections cause a debilitating disease with acute febrile illness and long-term polyarthralgia. Acute and chronic symptoms impose a major economic burden to he...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v11040322
更新日期:2019-04-03 00:00:00
abstract::The Pneumoviridae family includes human metapneumovirus (HMPV) and human orthopneumovirus, which is also known as a respiratory syncytial virus (HRSV). These are large enveloped, negative single-strand RNA viruses. HMPV and HRSV are the human members, which commonly infect children. HMPV, which was discovered in 2001,...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12070781
更新日期:2020-07-20 00:00:00
abstract::Bovine viral diarrhea virus (BVDV) is an important pathogen belonging to the Pestivirus genus, Flaviviridae family, which comprises viral species that causes an economic impact in animal production. Cattle are the natural host of BVDV and the main source of infection for pigs and other animal species. Due to its antig...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v12060600
更新日期:2020-05-31 00:00:00
abstract::Pteropine orthoreovirus (PRV; Reoviridae: Spinareovirinae) is an emerging bat-borne zoonotic virus that causes influenza-like illness (ILI). PRV has thus far been found only in Australia and Asia, where diverse old-world fruit bats (Pteropodidae) serve as hosts. In this study, we report the discovery of PRV in Africa,...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12070740
更新日期:2020-07-09 00:00:00
abstract::: African swine fever is a devastating hemorrhagic infectious disease, which affects domestic and wild swines (Susscrofa) of all breeds and ages, with a high lethality of up to 90-100% in naïve animals. The causative agent, African swine fever virus (ASFV), is a large and complex double-stranded DNA arbovirus which is...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v11100882
更新日期:2019-09-20 00:00:00
abstract::In rabies diagnosis, it is essential to count on a rapid test to give a quick response. The combined sensitivity and robustness of the TaqMan RT-PCR assays (qRT-PCR) have made these methods a valuable alternative for rabies virus (RABV) detection. We conducted a study to compare the applicability of two widely used qR...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v13010023
更新日期:2020-12-25 00:00:00
abstract::Several human monoclonal antibodies (hmAbs) and antibody fragments, including the best characterized in terms of structure-function b12 and Fab X5, exhibit relatively potent and broad HIV-1 neutralizing activity. However, the elicitation of b12 or b12-like antibodies in vivo by vaccine immunogens based on the HIV-1 en...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v1030802
更新日期:2009-12-01 00:00:00
abstract::Geminiviruses are important plant pathogens that affect crops around the world. In some geminivirus-host interactions, infected plants show recovery, a phenomenon characterized by symptom disappearance in newly emerging leaves. In pepper-Pepper golden mosaic virus (PepGMV) interaction, the host recovery process involv...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12030286
更新日期:2020-03-05 00:00:00
abstract::Hematopoietic stem cell transplantation from anti-cytomegalovirus immunoglobulin G (anti-CMV-IgG) positive donors facilitated immunological recovery post-transplant, which may indicate that chronic CMV infection has an effect on the immune system. This can be seen in the recipients after reconstitution with donor lymp...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v7031391
更新日期:2015-03-23 00:00:00
abstract::MicroRNAs (miRNAs) are non-coding but functional RNA molecules of 21-25 nucleotides in length. MiRNAs play significant regulatory roles in diverse plant biological processes. In order to decipher the relationship between nbe-miR1919c-5p and the accumulations of tobacco curly shoot virus (TbCSV) and its betasatellite (...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12040392
更新日期:2020-04-01 00:00:00
abstract::Micro RNAs (miRNAs) are a class of small non-coding single-stranded RNA, which play an important role in modulating host-Influenza A virus (IAV) crosstalk. The interplay between influenza and miRNA interaction is defined by a plethora of complex mechanisms, which are not fully understood yet. Here, we demonstrate that...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12040444
更新日期:2020-04-15 00:00:00
abstract::Begomoviruses are one of the major groups of plant viruses with an important economic impact on crop production in tropical and subtropical regions. The global spread of its polyphagous vector, the whitefly Bemisia tabaci, has contributed to the emergence and diversification of species within this genus. In this study...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v11040327
更新日期:2019-04-04 00:00:00
abstract::The progressive loss of immunological memory during aging correlates with a reduced proliferative capacity and shortened telomeres of T cells. Growing evidence suggests that this phenotype is recapitulated during chronic viral infection. The antigenic volume imposed by persistent and latent viruses exposes the immune ...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v9100289
更新日期:2017-10-05 00:00:00
abstract::Research on human enteroviruses has resulted in the identification of more than 100 enterovirus types, which use more than 10 protein receptors and/or attachment factors required in cell binding and initiation of the replication cycle. Many of these "viral" receptors are overexpressed in cancer cells. Receptor binding...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v8030057
更新日期:2016-02-23 00:00:00
abstract::Hepatitis C is a major global health burden with an estimated 160 million infected individuals worldwide. This long-term disease evolves slowly, often leading to chronicity and potentially to liver failure. There is no anti-HCV vaccine, and, until recently, the only treatment available, based on pegylated interferon a...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v4102197
更新日期:2012-10-17 00:00:00
abstract::Persistent circulative transmission of plant viruses involves complex interactions between the transmitted virus and its insect vector. Several studies have shown that insect vector proteins are involved in the passage and the transmission of the virus. Interestingly, proteins expressed by bacterial endosymbionts that...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v5061516
更新日期:2013-06-19 00:00:00
abstract::The chikungunya virus (CHIKV) is an emerging pathogen with widespread distribution in regions of Africa, India, and Asia that threatens to spread into temperate climates with the introduction of its major vector, Aedes albopictus. CHIKV causes a disease frequently misdiagnosed as dengue fever, with potentially life-th...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v8060163
更新日期:2016-06-10 00:00:00
abstract::The Bunyaviridae is a family of arboviruses including both plant- and vertebrate-infecting representatives. The Tospovirus genus accommodates plant-infecting bunyaviruses, which not only replicate in their plant host, but also in their insect thrips vector during persistent propagative transmission. For this reason, t...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v8070208
更新日期:2016-07-23 00:00:00