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The two phases of regulated exocytosis in permeabilized pancreatic acini are modulated differently by heterotrimeric G-proteins.

Abstract：

:In this study we examined the influence on AlF4- and GTP gamma S on amylase secretion from alpha toxin permeabilized pancreatic acini. AlF4- only activates heterotrimeric G-proteins, whereas GTP gamma S activates both small ras-like GTP-binding proteins and heterotrimeric G-proteins (Kahn, R. A., J. Biol. Chem., 266, 15595-15597, 1991). GTP gamma S, but not AlF4-, significantly stimulated Ca2(+)-independent amylase secretion, suggesting that a small GTP-binding protein controls regulated exocytosis distal to the site of action of Ca2+. In contrast, both AlF4- and GTP gamma S modulated Ca(2+)-dependent amylase secretion. AlF4- and GTP gamma S stimulated the initial rapid, ATP-independent, phase of Ca(2+)-dependent secretion but inhibited the second slower sustained, ATP-dependent, phase of release. There were significant differences in the GTP gamma S requirements for the stimulation and inhibition of Ca(2+)-dependent amylase secretion, consistent with GTP gamma S activating separate heterotrimeric G-proteins to modulate each phase of the Ca(2+)-dependent secretory response. Our studies also indicated that neither G-protein is a member of the Gi/o class of heterotrimeric G-proteins.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Padfield PJ,Panesar N

doi

10.1006/bbrc.1998.8350

subject

Has Abstract

pub_date

1998-04-17 00:00:00

pages

332-6

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(98)98350-4

journal_volume

245

pub_type

杂志文章

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