当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Phosphorylation at the N-terminal finger subdomain of a viral RNA-dependent RNA polymerase.

Phosphorylation at the N-terminal finger subdomain of a viral RNA-dependent RNA polymerase.

Abstract：

:The RNA-dependent RNA polymerase (RdRP) of the Hepatitis C virus (HCV), named NS5B, is phosphorylated by the cellular protein kinase C-related kinase 2 (PRK2) at two serine residues (Ser29 and Ser42) of the finger subdomain (genotype 1b). Herein, using bioinformatics, we selected four potential phosphorylation residues (Ser46, Ser76, Ser96 and Ser112) of NS5B (genotype 2a) for study. Whereas the NS5B Ser46D and Ser76D substitutions seemed to improve polymerase activity, the Ser96D mutation decreased colony formation efficiency. Active WT NS5B was utilized in in vitro kinase assays, and phosphopeptides were analyzed by mass spectrometry. Interestingly, the data indicated that both the NS5B Ser29 and Ser76 residues resulted phosphorylated. Thus, as Ser76 is absolutely conserved across HCV genotypes, our results confirmed the relevance of these sites for both genotypes and suggested that Ser76 becomes phosphorylated by a cellular kinase different from PRK2. By molecular dynamic simulations, we show that new interactions between space-adjacent amino acid chains could be established by the presence of a di-anionic phosphate group on the analyzed serines to possibly modify RNA polymerase activity. Together, our data present novel evidence on the complex regulation at the finger subdomain of HCV NS5B via phosphorylation.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Hernández S,Figueroa D,Correa S,Díaz A,Aguayo D,Villanueva RA

doi

10.1016/j.bbrc.2015.08.082

subject

Has Abstract

pub_date

2015-10-09 00:00:00

pages

21-7

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(15)30472-1

journal_volume

466

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • NIH 3T3 cells transfected with human tumor DNA lose the transformed phenotype when treated with swainsonine.

    abstract：:Phenotypic expression of transformation was inhibited by swainsonine at concentrations which affect the late stages of glycoprotein processing but not growth of cells. In the presence of swainsonine, NIH 3T3 fibroblasts transfected with human tumor DNA (al-l) no longer grew in soft agar or expressed complex type oligo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(87)90280-4

    authors： DeSantis R,Santer UV,Glick MC

    更新日期：1987-01-30 00:00:00

  • AAV-mediated delivery of the transcription factor XBP1s into the striatum reduces mutant Huntingtin aggregation in a mouse model of Huntington's disease.

    abstract：:Huntington's disease (HD) is caused by mutations that expand a polyglutamine region in the amino-terminal domain of Huntingtin (Htt), leading to the accumulation of intracellular inclusions and progressive neurodegeneration. Recent reports indicate the engagement of endoplasmic reticulum (ER) stress responses in human...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.03.033

    authors： Zuleta A,Vidal RL,Armentano D,Parsons G,Hetz C

    更新日期：2012-04-13 00:00:00

  • Suppression of HPV E6 and E7 expression by BAF53 depletion in cervical cancer cells.

    abstract：:Deregulation of the expression of human papillomavirus (HPV) oncogenes E6 and E7 plays a pivotal role in cervical carcinogenesis because the E6 and E7 proteins neutralize p53 and Rb tumor suppressor pathways, respectively. In approximately 90% of all cervical carcinomas, HPVs are found to be integrated into the host g...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.07.098

    authors： Lee K,Lee AY,Kwon YK,Kwon H

    更新日期：2011-08-26 00:00:00

  • Monte Carlo studies of lipid chains and gramicidin A in a model membrane.

    abstract：:The Monte Carlo method has been used to simulate the equilibrium properties of a planar array of 94 saturated lipid chains and one monomer of Gramicidin A. Chains are free to move laterally in the layer plane and to change conformation via gauche rotations and long axis rotations in a continuum. All non-hydrogen atoms...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)91025-5

    authors： Xing J,Scott HL

    更新日期：1989-11-30 00:00:00

  • Implication of pH in the catalytic properties of anthrax lethal factor.

    abstract：:The anthrax lethal factor (LF) is a Zn(2+)-endopeptidase specific for mitogen-activated protein kinase kinases (MAPKKs), which are cleaved within their N-terminal region. Much line of effort was carried out to elucidate the catalytic activity of LF for designing the inhibitor and to understand the cellular mechanism o...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2003.11.110

    authors： Kim J,Chai YG,Yoon MY

    更新日期：2004-01-02 00:00:00

  • A histone 1-like antigen is a component of the nuclear envelope.

    abstract：:Rabbit antibodies have been raised against rat liver nuclear envelopes. An enzyme-linked immunosorbent assay (ELISA) demonstrated high titer antiserum specific for the nuclear envelope preparation. Immunocytochemical studies showed that the antiserum stained the nuclear envelopes, but not intra-nuclear components of H...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(88)80895-7

    authors： Venkatraman JT,Gohill J,Fritzler MJ,Lefebvre YA

    更新日期：1988-10-31 00:00:00

  • Retardation of cell growth by avian reovirus p17 through the activation of p53 pathway.

    abstract：:The second open reading frame of avian reovirus S1 gene segment encodes a 17 kDa non-structural protein, named p17. The biological role of p17 is fully unknown so far. Using trypan blue dye exclusion and MTT assay, we demonstrated that the ectopic expression of p17 results in the reduction of viable cell number and ce...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.08.149

    authors： Liu HJ,Lin PY,Lee JW,Hsu HY,Shih WL

    更新日期：2005-10-21 00:00:00

  • Yeast ornithine decarboxylase and antizyme form a 1:1 complex in vitro: purification and characterization of the inhibitory complex.

    abstract：:Saccharomyces cerevisiae antizyme (AZ) resembles mammalian AZ in its mode of synthesis by translational frameshifting and its ability to inhibit and facilitate the degradation of ornithine decarboxylase (ODC). Despite many studies on the interaction of AZ and ODC, the ODC:AZ complex has not been purified from any sour...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.01.113

    authors： Chattopadhyay MK,Fernandez C,Sharma D,McPhie P,Masison DC

    更新日期：2011-03-11 00:00:00

  • Ancient DNA in human bone remains from Pompeii archaeological site.

    abstract：:aDNA extraction and amplification procedures have been optimized for Pompeian human bone remains whose diagenesis has been determined by histological analysis. Single copy genes amplification (X and Y amelogenin loci and Y specific alphoid repeat sequences) have been performed and compared with anthropometric data on ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.8881

    authors： Cipollaro M,Di Bernardo G,Galano G,Galderisi U,Guarino F,Angelini F,Cascino A

    更新日期：1998-06-29 00:00:00

  • Cyclosporin A blocks induction of tumor necrosis factor-alpha in human B lymphocytes.

    abstract：:The effects of cyclosporin A (CSP) on tumor necrosis factor-alpha (TNF-alpha) RNA levels and protein production in human B cells and a B cell line were studied. The ability of CSP to block induction of RNA was compared to its inhibition of protein production in response to anti-IgM, phorbol ester (PMA) and platelet-ac...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.2470

    authors： Smith CS,Ortega G,Parker L,Shearer WT

    更新日期：1994-10-14 00:00:00

  • GOLGA2 loss causes fibrosis with autophagy in the mouse lung and liver.

    abstract：:Autophagy is a biological recycling process via the self-digestion of organelles, proteins, and lipids for energy-consuming differentiation and homeostasis. The Golgi serves as a donor of the double-membraned phagophore for autophagosome assembly. In addition, recent studies have demonstrated that pulmonary and hepati...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.11.049

    authors： Park S,Kim S,Kim MJ,Hong Y,Lee AY,Lee H,Tran Q,Kim M,Cho H,Park J,Kim KP,Park J,Cho MH

    更新日期：2018-01-01 00:00:00

  • The chemokine CXCL13 (BCA-1) inhibits FGF-2 effects on endothelial cells.

    abstract：:Several chemokines, belonging to both the CXC and CC classes, act as positive or negative regulators of angiogenesis. We sought to investigate the role of CXCL13, B cell-attracting chemokine 1 (BCA-1), also known as B-lymphocyte chemoattractant (BLC), on endothelial cell functions. We tested the effect of CXCL13 on HU...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.5924

    authors： Spinetti G,Camarda G,Bernardini G,Romano Di Peppe S,Capogrossi MC,Napolitano M

    更新日期：2001-11-23 00:00:00

  • Angiotensin II stimulates the proliferation of osteoblast-rich populations of cells from rat calvariae.

    abstract：:We examined the effects of angiotensin II (Ang II) on the proliferation of osteoblast-rich populations of cells obtained from calvariae of newborn rat. Addition of Ang II to the culture medium caused dose-dependent increases in the rate of DNA synthesis. Such increases were completely inhibited by the addition of DuP7...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.5914

    authors： Hiruma Y,Inoue A,Hirose S,Hagiwara H

    更新日期：1997-01-03 00:00:00

  • microRNA-495 promotes bladder cancer cell growth and invasion by targeting phosphatase and tensin homolog.

    abstract：:Accumulating evidence has linked deregulation of microRNA-495 (miR-495) to tumorigenesis; however, its function in tumor progression is controversial. This work was undertaken to explore the expression and biological roles of miR-495 in bladder cancer. The expression of miR-495 was examined in 67 pairs of bladder canc...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.01.019

    authors： Tan M,Mu X,Liu Z,Tao L,Wang J,Ge J,Qiu J

    更新日期：2017-02-05 00:00:00

  • Overexpression of human WNK1 increases paracellular chloride permeability and phosphorylation of claudin-4 in MDCKII cells.

    abstract：:Pseudohypoaldosteronism type II (PHAII), an autosomal dominant disorder characterized by hypertension, hyperkalemia, and hyperchloremic acidosis, is reportedly due to mutations in WNK1 and WNK4 kinase genes. However, the pathogenesis of the disease remains unknown. Mutations in the WNK1 gene are the deletions in the f...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.08.101

    authors： Ohta A,Yang SS,Rai T,Chiga M,Sasaki S,Uchida S

    更新日期：2006-10-20 00:00:00

  • Molecular modeling of the human multidrug resistance protein 1 (MRP1/ABCC1).

    abstract：:Multidrug resistance protein 1 (MRP1/ABCC1) is a 190kDa member of the ATP-binding cassette (ABC) superfamily of transmembrane transporters that is clinically relevant for its ability to confer multidrug resistance by actively effluxing anticancer drugs. Knowledge of the atomic structure of MRP1 is needed to elucidate ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.10.141

    authors： DeGorter MK,Conseil G,Deeley RG,Campbell RL,Cole SP

    更新日期：2008-01-04 00:00:00

  • Arabidopsis AAL-toxin-resistant mutant atr1 shows enhanced tolerance to programmed cell death induced by reactive oxygen species.

    abstract：:The fungal AAL-toxin triggers programmed cell death (PCD) through perturbations of sphingolipid metabolism in AAL-toxin-sensitive plants. While Arabidopsis is relatively insensitive to the toxin, the loh2 mutant exhibits increased susceptibility to AAL-toxin due to the knockout of a gene involved in sphingolipid metab...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.08.056

    authors： Gechev TS,Ferwerda MA,Mehterov N,Laloi C,Qureshi MK,Hille J

    更新日期：2008-10-31 00:00:00

  • Differential sublocalization of the dynamin-related protein OPA1 isoforms in mitochondria.

    abstract：:OPA1 is a cause gene for autosomal dominant optic atrophy and possesses eight alternative splicing variants. Here, we identified two isoforms of OPA1 proteins in HeLa cells and examined their submitochondrial localization and complex formations. RT-PCR shows that HeLa cells mainly express isoforms 7 and 1 of OPA1. Sin...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(02)02874-7

    authors： Satoh M,Hamamoto T,Seo N,Kagawa Y,Endo H

    更新日期：2003-01-10 00:00:00

  • Expression of PrP mRNA is regulated by a fragment of MRP8 in human fibroblasts.

    abstract：:Prion protein (PrPc) is expressed in many tissues, both in human and animals. The scrapie isoform of PrPc has been shown to cause neurodegeneration. In other studies it has been demonstrated that overexpression of the PrP gene can result in nonneuronal tissue degradation. Little is known, however, about the normal fun...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.6575

    authors： Kniazeva M,Orman R,Terranova VP

    更新日期：1997-05-08 00:00:00

  • The ABCA1 transporter functions on the basolateral surface of hepatocytes.

    abstract：:ABCA1 on the cell surface and in endosomes plays an essential role in the cell-mediated lipidation of apoA-I to form nascent HDL. Our previous studies of transgenic mice overexpressing ABCA1 suggested that ABCA1 in the liver plays a major role in regulating plasma HDL levels. The site of function of ABCA1 in the polar...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(02)02274-x

    authors： Neufeld EB,Demosky SJ Jr,Stonik JA,Combs C,Remaley AT,Duverger N,Santamarina-Fojo S,Brewer HB Jr

    更新日期：2002-10-04 00:00:00

  • Two genetically different MU-NANA neuraminidases in human leucocytes.

    abstract：:Human leucocytes contain two different MU-NANA neuraminidases, which can be distinguished by Concanavalin A binding. The Con A binding form is predominant in lymphocytes (more than 80%) and the non-binding form predominates in granulocytes. The pH optima of both these neuraminidases as well as their subcellular locali...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(83)91224-x

    authors： Verheijen FW,Janse HC,van Diggelen OP,Bakker HD,Loonen MC,Durand P,Galjaard H

    更新日期：1983-12-16 00:00:00

  • Insights into function of PSI domains from structure of the Met receptor PSI domain.

    abstract：:PSI domains are cysteine-rich modules found in extracellular fragments of hundreds of signaling proteins, including plexins, semaphorins, integrins, and attractins. Here, we report the solution structure of the PSI domain from the human Met receptor, a receptor tyrosine kinase critical for proliferation, motility, and...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.06.132

    authors： Kozlov G,Perreault A,Schrag JD,Park M,Cygler M,Gehring K,Ekiel I

    更新日期：2004-08-13 00:00:00

  • Ectopic expression of the maize chromosomal HMGB1 protein causes defects in root development of tobacco seedlings.

    abstract：:Chromatin-associated high mobility group (HMG) proteins of the HMGB family are versatile architectural factors assisting various DNA-dependent processes such as transcription and recombination. Here, transgenic tobacco lines were generated that ectopically express the maize HMGB1 protein, as detected by immunoblot ana...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.03.193

    authors： Lichota J,Ritt C,Grasser KD

    更新日期：2004-05-21 00:00:00

  • O-alkyl lipid synthesis: the mechanism of the acyl dihydroxyacetone phosphate fatty acid exchange reaction.

    abstract：:We have previously provided evidence for a mechanism by which acyl DHAP is converted enzymatically to O-alkyl DHAP. This mechanism involves, in part, the formation of an endiol of acyl DHAP, loss of the fatty acid by splitting of the DHAP carbon-1 to oxygen bond and the gain of a long chain fatty alcohol. It has been ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(87)91561-0

    authors： Friedberg SJ,Weintraub ST,Peterson D,Satsangi N

    更新日期：1987-06-30 00:00:00

  • The number of accessible SH-groups in Escherichia coli membrane vesicles is increased by ATP or by formate.

    abstract：:The number of accessible SH-groups was determined in membrane vesicles prepared from Escherichia coli growing in fermentation conditions at slightly alkaline pH on glucose with or without added formate. Addition of ATP or formate to the vesicles caused a approximately 1.4-fold increase in the number of accessible SH-g...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(03)01460-8

    authors： Mnatsakanyan N,Poladian A,Bagramyan K,Trchounian A

    更新日期：2003-08-29 00:00:00

  • Dystroglycan depletion inhibits the functions of differentiated HL-60 cells.

    abstract：:Dystroglycan has recently been characterized in blood tissue cells, as part of the dystrophin glycoprotein complex but to date nothing is known of its role in the differentiation process of neutrophils. We have investigated the role of dystroglycan in the human promyelocytic leukemic cell line HL-60 differentiated to ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.04.110

    authors： Martínez-Zárate AD,Martínez-Vieyra I,Alonso-Rangel L,Cisneros B,Winder SJ,Cerecedo D

    更新日期：2014-06-06 00:00:00

  • A coarse-grained Langevin molecular dynamics approach to de novo protein structure prediction.

    abstract：:De novo prediction of protein structures, the prediction of structures from amino acid sequences which are not similar to those of hitherto resolved structures, has been one of the major challenges in molecular biophysics. In this paper, we develop a new method of de novo prediction, which combines the fragment assemb...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.02.048

    authors： Sasaki TN,Cetin H,Sasai M

    更新日期：2008-05-02 00:00:00

  • Electrospinning of silica nanoparticles-entrapped nanofibers for sustained gentamicin release.

    abstract：:Sustained antibiotic release is important to prevent the infections after surgical treatments such as guided tissue regeneration (GTR). Electrospinning provides a simple way to fabricate nanofibers for drug delivery. In this study, a simple method to achieve sustained antibiotic release by introducing mesoporous silic...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.06.163

    authors： Chen X,Xu C,He H

    更新日期：2019-09-03 00:00:00

  • Folding-dependent in vitro protein splicing of the Saccharomyces cerevisiae VMA1 protozyme.

    abstract：:VMA1 translational product undergoes excision of a 50-kDa intervening segment (VDE: VMA1-derived endonuclease) and religation of the flanking regions to create a 69-kDa catalytic subunit of vacuolar membrane H+-ATPase. VDEs conjugated with polypeptides at both N- and C-terminal ends were expressed in Escherichia coli ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.0826

    authors： Kawasaki M,Makino S,Matsuzawa H,Satow Y,Ohya Y,Anraku Y

    更新日期：1996-05-24 00:00:00

  • Chronic hepatitis C viral infection reduces NK cell frequency and suppresses cytokine secretion: Reversion by anti-viral treatment.

    abstract：:Impaired activity of NK (natural killer) cells has been proposed as a mechanism contributing to viral persistence and chronic infection in hepatitis C (HCV) infection. We aimed to assess the impact of HCV infection on NK cells regarding frequency, subset distribution, and cytotoxic and cytokine secretion functions, as...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.02.008

    authors： Dessouki O,Kamiya Y,Nagahama H,Tanaka M,Suzu S,Sasaki Y,Okada S

    更新日期：2010-03-05 00:00:00